Incidental Mutation 'R3017:Odf1'
ID 257674
Institutional Source Beutler Lab
Gene Symbol Odf1
Ensembl Gene ENSMUSG00000061923
Gene Name outer dense fiber of sperm tails 1
Synonyms
Accession Numbers
Essential gene? Probably non essential (E-score: 0.062) question?
Stock # R3017 (G1)
Quality Score 225
Status Not validated
Chromosome 15
Chromosomal Location 38219447-38226979 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 38226675 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 192 (T192A)
Ref Sequence ENSEMBL: ENSMUSP00000080632 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000081966]
AlphaFold no structure available at present
Predicted Effect unknown
Transcript: ENSMUST00000081966
AA Change: T192A
SMART Domains Protein: ENSMUSP00000080632
Gene: ENSMUSG00000061923
AA Change: T192A

DomainStartEndE-ValueType
coiled coil region 1 28 N/A INTRINSIC
internal_repeat_1 35 47 2.51e-7 PROSPERO
internal_repeat_1 45 57 2.51e-7 PROSPERO
low complexity region 67 96 N/A INTRINSIC
Pfam:HSP20 110 187 4.9e-8 PFAM
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.4%
  • 10x: 96.8%
  • 20x: 93.4%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. The human outer dense fibers contains at least 10 major proteins and this gene encodes the main protein. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit male infertility associated with asthenozoospermia, oligozoospermia, detached sperm head and absent acrosome reaction. Mice heterozygous for this allele exhibit asthenozoospermia without altered fertility. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 16 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aox4 T C 1: 58,274,363 (GRCm39) V383A probably benign Het
Aplp1 A T 7: 30,135,396 (GRCm39) probably null Het
Bahd1 C T 2: 118,746,887 (GRCm39) P169S probably damaging Het
Gmnc G A 16: 26,779,165 (GRCm39) H281Y probably benign Het
Gusb T C 5: 130,029,325 (GRCm39) D152G probably damaging Het
Kcnk4 A T 19: 6,905,162 (GRCm39) V163E probably damaging Het
Lcmt1 T G 7: 123,029,359 (GRCm39) L327W probably damaging Het
Med18 G A 4: 132,187,128 (GRCm39) R124C probably damaging Het
Pitpna C T 11: 75,483,016 (GRCm39) R8W probably damaging Het
Pkn1 A G 8: 84,396,799 (GRCm39) Y951H probably benign Het
Rad18 T C 6: 112,658,327 (GRCm39) T193A possibly damaging Het
Ruvbl2 G T 7: 45,071,588 (GRCm39) L432I probably damaging Het
Slco1a4 T C 6: 141,758,396 (GRCm39) probably null Het
Tlr11 T A 14: 50,600,178 (GRCm39) C721* probably null Het
Tubgcp6 A T 15: 88,987,285 (GRCm39) C1229* probably null Het
Vmn2r114 ATTT ATT 17: 23,509,906 (GRCm39) probably null Het
Other mutations in Odf1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02108:Odf1 APN 15 38,226,623 (GRCm39) nonsense probably null
R4967:Odf1 UTSW 15 38,226,652 (GRCm39) missense probably damaging 0.98
R5134:Odf1 UTSW 15 38,226,393 (GRCm39) missense possibly damaging 0.68
R5319:Odf1 UTSW 15 38,219,863 (GRCm39) missense probably benign 0.00
R5951:Odf1 UTSW 15 38,226,531 (GRCm39) missense probably damaging 0.99
R7095:Odf1 UTSW 15 38,219,803 (GRCm39) missense possibly damaging 0.86
R7197:Odf1 UTSW 15 38,219,794 (GRCm39) missense probably benign 0.00
R7961:Odf1 UTSW 15 38,226,840 (GRCm39) missense unknown
R8009:Odf1 UTSW 15 38,226,840 (GRCm39) missense unknown
R9789:Odf1 UTSW 15 38,219,716 (GRCm39) missense probably benign 0.03
Z1088:Odf1 UTSW 15 38,219,918 (GRCm39) missense probably benign 0.41
Predicted Primers PCR Primer
(F):5'- TCAAGTCAAAGTGCGCGTG -3'
(R):5'- CTGGCTGGAGTACTGACTTC -3'

Sequencing Primer
(F):5'- AAGATGGGAAGGTCTGCGTGTC -3'
(R):5'- CTGGAGTACTGACTTCTGCTAAGAC -3'
Posted On 2015-01-11