Mutagenetix > Incidental Mutation

Incidental Mutation 'R3161:Wls'

258156

Institutional Source

Beutler Lab

Gene Symbol

Wls

Ensembl Gene

ENSMUSG00000028173

Gene Name

wntless WNT ligand secretion mediator

Synonyms

5031439A09Rik, Gpr177

MMRRC Submission

040612-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R3161 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

159839672-159938664 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 159897436 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Phenylalanine at position 162 (C162F)

Ref Sequence

ENSEMBL: ENSMUSP00000143475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068952] [ENSMUST00000198878] [ENSMUST00000200191]

AlphaFold

Q6DID7

Predicted Effect

probably damaging
Transcript: ENSMUST00000068952
AA Change: C162F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000067898
Gene: ENSMUSG00000028173
AA Change: C162F

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	36	47	N/A	INTRINSIC
Pfam:MIG-14_Wnt-bd	178	496	3.7e-97	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197328

Predicted Effect

probably damaging
Transcript: ENSMUST00000198878
AA Change: C162F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000143475
Gene: ENSMUSG00000028173
AA Change: C162F

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	36	47	N/A	INTRINSIC
Pfam:MIG-14_Wnt-bd	177	497	2.8e-94	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000200191

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200571

Meta Mutation Damage Score

0.9412

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.3%

Validation Efficiency

98% (54/55)

MGI Phenotype

PHENOTYPE: Mice homozygous for null alleles exhibit impaired body axis and triploblastic development dying prior to E10.5. Mice homozygous for a floxed allele activated in keratinocytes exhibit a psoriasiform dermatitis-like phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	T	C	5: 63,896,490		probably benign	Het
1700066M21Rik	T	A	1: 57,383,075	N203K	probably benign	Het
1700125H20Rik	A	G	11: 85,173,284	S84G	probably damaging	Het
Adcy9	A	G	16: 4,311,588	L715P	probably damaging	Het
Adgrl2	G	A	3: 148,817,551	L1354F	probably damaging	Het
Amer2	A	G	14: 60,378,551	D65G	probably damaging	Het
Aox2	G	T	1: 58,304,438	V427L	possibly damaging	Het
Atad2b	C	A	12: 4,939,689	N133K	possibly damaging	Het
Bptf	A	T	11: 107,074,476	D1182E	probably damaging	Het
Camk1g	T	C	1: 193,359,807	T45A	possibly damaging	Het
Caps2	C	A	10: 112,182,486	Y180*	probably null	Het
Cfap54	T	A	10: 93,045,278	K349N	probably damaging	Het
Ciz1	A	G	2: 32,370,063	D207G	probably benign	Het
Copa	T	A	1: 172,091,233	C127S	probably damaging	Het
Crabp2	A	T	3: 87,952,177	K45*	probably null	Het
Daam1	C	A	12: 71,947,098	T425K	unknown	Het
Dapk2	T	G	9: 66,254,611	V267G	probably damaging	Het
Disp1	T	C	1: 183,087,242	K1205E	probably benign	Het
Dlg4	G	C	11: 70,017,225	R4T	probably damaging	Het
Fbf1	A	G	11: 116,148,220	I743T	probably damaging	Het
Fen1	A	G	19: 10,200,291	L263P	probably damaging	Het
G6pc2	A	G	2: 69,220,112	N27S	probably damaging	Het
Garnl3	A	T	2: 33,034,711	N246K	probably damaging	Het
Gm7337	A	C	5: 87,851,557		noncoding transcript	Het
Gpr152	A	G	19: 4,142,714	T85A	probably benign	Het
Hnrnpu	T	C	1: 178,331,125		probably benign	Het
Ighv1-81	C	G	12: 115,920,329	E101Q	probably benign	Het
Ipo9	T	C	1: 135,409,476	T174A	probably benign	Het
Myo9a	C	T	9: 59,832,315		probably benign	Het
Nup155	G	T	15: 8,148,383	R1083S	possibly damaging	Het
Olfr1351	C	A	10: 79,017,604	T94N	probably benign	Het
Olfr213	C	T	6: 116,540,846	A131V	probably damaging	Het
Olfr262	T	C	19: 12,241,496	H55R	probably benign	Het
Olfr739	T	A	14: 50,425,031	C171S	probably damaging	Het
Phyh	T	A	2: 4,937,671		probably benign	Het
Pkp4	G	T	2: 59,308,105	R233M	probably damaging	Het
Plcb1	A	T	2: 135,335,482	Q578L	probably benign	Het
Ppil3	A	T	1: 58,434,414	N92K	probably benign	Het
Prokr1	C	T	6: 87,588,431	R144H	probably damaging	Het
Psap	T	C	10: 60,277,753	L4P	possibly damaging	Het
Rai14	T	C	15: 10,633,164	T47A	possibly damaging	Het
Rps2	G	T	17: 24,720,978	A129S	probably benign	Het
Sult2a4	T	C	7: 13,989,471	T40A	probably benign	Het
Tacr2	A	G	10: 62,265,245	D378G	probably benign	Het
Topaz1	T	C	9: 122,749,381	I452T	probably benign	Het
Ttn	A	G	2: 76,833,237		probably benign	Het
Vmn2r115	T	A	17: 23,357,024	M532K	possibly damaging	Het
Vmn2r117	A	G	17: 23,460,378	L624P	probably damaging	Het
Vstm5	T	G	9: 15,257,298	S53A	probably benign	Het
Wipf1	C	T	2: 73,434,949	E437K	probably damaging	Het
Yeats2	T	C	16: 20,193,645	V531A	probably damaging	Het
Zfp868	A	G	8: 69,612,085	S200P	probably benign	Het

Other mutations in Wls

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01924:Wls	APN	3	159901443	nonsense	probably null
IGL02065:Wls	APN	3	159911356	missense	probably damaging	0.99
IGL02073:Wls	APN	3	159907253	critical splice donor site	probably null
IGL02133:Wls	APN	3	159897370	missense	probably damaging	1.00
R0374:Wls	UTSW	3	159897437	nonsense	probably null
R0561:Wls	UTSW	3	159873068	missense	probably benign	0.32
R1697:Wls	UTSW	3	159897358	missense	probably benign	0.12
R1791:Wls	UTSW	3	159911813	missense	probably benign	0.17
R2444:Wls	UTSW	3	159907230	missense	probably damaging	1.00
R4285:Wls	UTSW	3	159934266	missense	probably benign
R4468:Wls	UTSW	3	159872927	missense	probably damaging	0.96
R4472:Wls	UTSW	3	159897383	missense	probably benign	0.01
R4782:Wls	UTSW	3	159897445	missense	probably benign	0.03
R4799:Wls	UTSW	3	159897445	missense	probably benign	0.03
R4809:Wls	UTSW	3	159897445	missense	probably benign	0.03
R5006:Wls	UTSW	3	159911791	missense	possibly damaging	0.68
R5212:Wls	UTSW	3	159873008	missense	probably benign	0.15
R5434:Wls	UTSW	3	159934340	missense	probably damaging	0.97
R5694:Wls	UTSW	3	159839987	missense	probably benign	0.01
R6315:Wls	UTSW	3	159934371	critical splice donor site	probably null
R7069:Wls	UTSW	3	159934329	missense	probably damaging	1.00
R7243:Wls	UTSW	3	159909765	missense	possibly damaging	0.49
R7529:Wls	UTSW	3	159873007	missense	probably benign	0.43
R7697:Wls	UTSW	3	159911318	missense	probably benign	0.21
R7842:Wls	UTSW	3	159873179	missense	probably benign	0.09
R8136:Wls	UTSW	3	159873124	missense	probably damaging	1.00
R8536:Wls	UTSW	3	159873111	missense	probably damaging	0.96
R8816:Wls	UTSW	3	159934292	missense	possibly damaging	0.89
R9074:Wls	UTSW	3	159909766	missense	possibly damaging	0.86

Predicted Primers

PCR Primer
(F):5'- GGCCTGTGAACATTTTGGAATG -3'
(R):5'- AGTCCTTCAAGAGCAGCTAAG -3'

Sequencing Primer
(F):5'- CATGTTTTCTCTTGCAGGAG -3'
(R):5'- TCAGTGGAAAGTCTAGCCTTTC -3'

Posted On

2015-01-23