Mutagenetix > Incidental Mutation

Incidental Mutation 'R3277:Or9s27'

258245

Institutional Source

Beutler Lab

Gene Symbol

Or9s27

Ensembl Gene

ENSMUSG00000046300

Gene Name

olfactory receptor family 9 subfamily S member 27

Synonyms

MOR208-4, Olfr1412, GA_x6K02T2R7CC-81165686-81164721

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R3277 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

92516054-92517019 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 92516535 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 161 (N161S)

Ref Sequence

ENSEMBL: ENSMUSP00000150943 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062964] [ENSMUST00000190505]

AlphaFold

Q8VET3

Predicted Effect

probably benign
Transcript: ENSMUST00000062964
AA Change: N161S

PolyPhen 2 Score 0.325 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000060291
Gene: ENSMUSG00000046300
AA Change: N161S

Domain	Start	End	E-Value	Type
transmembrane domain	10	27	N/A	INTRINSIC
Pfam:7tm_4	38	314	7.8e-47	PFAM
Pfam:7tm_1	48	321	1.4e-26	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000190505
AA Change: N161S

PolyPhen 2 Score 0.325 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204141

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921509C19Rik	T	C	2: 151,314,020 (GRCm39)	R553G	possibly damaging	Het
Adarb2	A	G	13: 8,802,663 (GRCm39)	N646S	probably damaging	Het
Adcy8	C	T	15: 64,571,008 (GRCm39)	G1242S	probably benign	Het
Ano9	T	A	7: 140,684,037 (GRCm39)	T543S	probably damaging	Het
Btnl10	A	G	11: 58,813,216 (GRCm39)	K282E	probably benign	Het
Btnl9	T	C	11: 49,060,503 (GRCm39)	D330G	probably damaging	Het
Ccdc178	G	T	18: 22,200,709 (GRCm39)	A416E	possibly damaging	Het
Cdx2	A	T	5: 147,240,002 (GRCm39)	S225T	probably benign	Het
Clca4b	T	C	3: 144,617,120 (GRCm39)	I843M	probably benign	Het
Cntn4	G	A	6: 106,414,925 (GRCm39)		probably null	Het
Cyp4f18	T	C	8: 72,747,044 (GRCm39)	D317G	possibly damaging	Het
Dennd4a	G	T	9: 64,796,275 (GRCm39)	R767L	probably damaging	Het
Dgkb	G	A	12: 38,134,216 (GRCm39)	V41M	probably damaging	Het
Duox1	T	C	2: 122,170,597 (GRCm39)	Y1206H	probably damaging	Het
Dync1i1	T	C	6: 5,972,211 (GRCm39)		probably null	Het
Fbxw2	T	C	2: 34,712,762 (GRCm39)	T100A	probably benign	Het
Fcgbp	C	A	7: 27,791,086 (GRCm39)	H782Q	probably damaging	Het
Flg2	A	T	3: 93,122,195 (GRCm39)	Q1455L	unknown	Het
Frrs1	T	C	3: 116,692,873 (GRCm39)	F49S	probably damaging	Het
Gli3	A	T	13: 15,900,567 (GRCm39)	Q1318L	probably benign	Het
Gm5592	A	G	7: 40,937,804 (GRCm39)	E362G	probably benign	Het
Gm7104	A	T	12: 88,252,498 (GRCm39)		noncoding transcript	Het
Gpatch2l	A	G	12: 86,291,089 (GRCm39)	T91A	possibly damaging	Het
Hacd4	T	C	4: 88,355,747 (GRCm39)	H46R	probably damaging	Het
Herc2	T	C	7: 55,803,176 (GRCm39)	V2175A	probably benign	Het
Hey1	T	C	3: 8,729,951 (GRCm39)	S169G	probably benign	Het
Hivep2	C	A	10: 14,004,713 (GRCm39)	T437K	probably benign	Het
Hlf	T	C	11: 90,236,661 (GRCm39)	K199E	probably damaging	Het
Hpgd	C	A	8: 56,751,448 (GRCm39)	A92E	probably damaging	Het
Hsp90aa1	C	A	12: 110,662,115 (GRCm39)		probably null	Het
Hsp90aa1	T	A	12: 110,662,114 (GRCm39)	M1L	possibly damaging	Het
Itgad	C	A	7: 127,790,153 (GRCm39)	H651N	possibly damaging	Het
Itgav	A	G	2: 83,606,886 (GRCm39)	D409G	probably damaging	Het
Kif2a	A	G	13: 107,113,264 (GRCm39)	I455T	probably damaging	Het
Klk14	G	A	7: 43,341,501 (GRCm39)	C51Y	probably damaging	Het
Lamc3	G	T	2: 31,798,637 (GRCm39)	G448C	probably damaging	Het
Ltbp1	G	A	17: 75,583,475 (GRCm39)	G425D	possibly damaging	Het
Ltbp1	T	A	17: 75,666,273 (GRCm39)		probably null	Het
Mag	C	T	7: 30,601,073 (GRCm39)		probably null	Het
Mdh1b	G	A	1: 63,750,690 (GRCm39)	T426M	possibly damaging	Het
Nr1h4	G	A	10: 89,314,650 (GRCm39)	T282I	possibly damaging	Het
Nsf	C	T	11: 103,821,578 (GRCm39)	E26K	possibly damaging	Het
Or4c1	C	T	2: 89,133,562 (GRCm39)	V125M	possibly damaging	Het
Or52k2	T	C	7: 102,253,783 (GRCm39)	V74A	possibly damaging	Het
Padi6	A	G	4: 140,462,700 (GRCm39)	L307P	probably damaging	Het
Parp9	T	C	16: 35,768,578 (GRCm39)	S20P	probably damaging	Het
Pdcd11	T	C	19: 47,101,703 (GRCm39)	F963L	probably damaging	Het
Pwp1	C	T	10: 85,717,943 (GRCm39)	L294F	probably benign	Het
Radil	A	G	5: 142,492,611 (GRCm39)	L339P	probably damaging	Het
Raver1	G	A	9: 20,990,573 (GRCm39)	P316S	possibly damaging	Het
Rell1	A	G	5: 64,084,330 (GRCm39)		probably null	Het
Rxrg	A	G	1: 167,463,269 (GRCm39)	D257G	possibly damaging	Het
Sema4c	C	T	1: 36,588,960 (GRCm39)	R722H	possibly damaging	Het
Sgk1	C	T	10: 21,872,500 (GRCm39)	R171W	probably damaging	Het
Spata7	A	G	12: 98,603,857 (GRCm39)	N75D	possibly damaging	Het
Ttc23l	A	G	15: 10,547,318 (GRCm39)	F99L	possibly damaging	Het
Unc13a	A	C	8: 72,082,339 (GRCm39)	C1642G	probably benign	Het
Usp36	C	T	11: 118,167,585 (GRCm39)		probably null	Het
Wrn	A	G	8: 33,807,582 (GRCm39)	M292T	probably damaging	Het
Zfp423	A	G	8: 88,508,959 (GRCm39)	Y462H	probably damaging	Het
Zscan5b	T	A	7: 6,234,345 (GRCm39)	Y124N	possibly damaging	Het
Zswim9	T	C	7: 13,011,196 (GRCm39)	T51A	possibly damaging	Het

Other mutations in Or9s27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Or9s27	APN	1	92,516,694 (GRCm39)	missense	possibly damaging	0.52
IGL02177:Or9s27	APN	1	92,516,479 (GRCm39)	missense	possibly damaging	0.96
IGL02507:Or9s27	APN	1	92,516,648 (GRCm39)	missense	possibly damaging	0.68
IGL02888:Or9s27	APN	1	92,516,925 (GRCm39)	missense	probably damaging	1.00
IGL03001:Or9s27	APN	1	92,516,273 (GRCm39)	missense	probably damaging	1.00
R1771:Or9s27	UTSW	1	92,516,837 (GRCm39)	missense	probably benign	0.03
R1780:Or9s27	UTSW	1	92,516,111 (GRCm39)	missense	probably benign	0.01
R2215:Or9s27	UTSW	1	92,516,708 (GRCm39)	missense	probably benign	0.00
R2437:Or9s27	UTSW	1	92,516,688 (GRCm39)	missense	possibly damaging	0.46
R3176:Or9s27	UTSW	1	92,516,535 (GRCm39)	missense	probably benign	0.32
R3177:Or9s27	UTSW	1	92,516,535 (GRCm39)	missense	probably benign	0.32
R3276:Or9s27	UTSW	1	92,516,535 (GRCm39)	missense	probably benign	0.32
R4475:Or9s27	UTSW	1	92,516,301 (GRCm39)	missense	probably benign	0.00
R4892:Or9s27	UTSW	1	92,516,643 (GRCm39)	missense	probably benign	0.05
R5910:Or9s27	UTSW	1	92,516,429 (GRCm39)	missense	probably damaging	1.00
R6808:Or9s27	UTSW	1	92,516,768 (GRCm39)	missense	probably damaging	0.99
R7130:Or9s27	UTSW	1	92,516,634 (GRCm39)	missense	probably benign	0.10
R7476:Or9s27	UTSW	1	92,516,986 (GRCm39)	missense	probably benign
R9695:Or9s27	UTSW	1	92,516,595 (GRCm39)	missense	probably benign	0.01
Z1088:Or9s27	UTSW	1	92,516,273 (GRCm39)	missense	probably damaging	1.00
Z1177:Or9s27	UTSW	1	92,516,100 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGCAATGGCCAACTTCTATTCC -3'
(R):5'- CAGGATGACTAATGTTGCCCC -3'

Sequencing Primer
(F):5'- ATGGCCAACTTCTATTCCTCCAAAG -3'
(R):5'- TGTTGCCCCCATTAGAATGAAAC -3'

Posted On

2015-01-23