Mutagenetix > Incidental Mutation

Incidental Mutation 'R3720:Tns3'

258861

Institutional Source

Beutler Lab

Gene Symbol

Tns3

Ensembl Gene

ENSMUSG00000020422

Gene Name

tensin 3

Synonyms

TEM6, F830010I22Rik, Tens1

MMRRC Submission

040711-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.371) question?

Stock #

R3720 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

8431652-8664535 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 8492999 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Tryptophan at position 455 (R455W)

Ref Sequence

ENSEMBL: ENSMUSP00000020695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020695]

AlphaFold

Q5SSZ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000020695
AA Change: R455W

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000020695
Gene: ENSMUSG00000020422
AA Change: R455W

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	1	171	5e-28	SMART
PTEN_C2	173	300	1.15e-48	SMART
low complexity region	854	864	N/A	INTRINSIC
low complexity region	1102	1126	N/A	INTRINSIC
SH2	1165	1268	1.32e-18	SMART
PTB	1301	1438	3.14e-24	SMART

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 97.0%
20x: 94.0%

Validation Efficiency

93% (40/43)

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit one third postnatal lethality, reduced body weight, growth retardation, smaller digestive tracts with defects in villi and enterocyte differentiation, abnormal lung morphology, and thinner bones with decreased chondrocyte proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Appl1	G	A	14: 26,927,844 (GRCm38)	T575M	probably damaging	Het
Atp2c1	A	T	9: 105,422,976 (GRCm38)	M708K	probably damaging	Het
C9	A	G	15: 6,483,119 (GRCm38)	T241A	possibly damaging	Het
Ccrl2	T	C	9: 111,056,364 (GRCm38)	D22G	probably benign	Het
Cd47	A	G	16: 49,867,842 (GRCm38)	I42V	probably benign	Het
Cntnap5c	T	C	17: 58,330,202 (GRCm38)	S1025P	probably benign	Het
Col8a1	T	C	16: 57,626,916 (GRCm38)	M744V	unknown	Het
Cstf3	A	G	2: 104,653,086 (GRCm38)		probably benign	Het
Dnah8	G	A	17: 30,854,898 (GRCm38)	R4514H	probably damaging	Het
Dnaic1	G	A	4: 41,602,615 (GRCm38)	R113H	probably damaging	Het
Fry	T	C	5: 150,454,572 (GRCm38)	S410P	probably damaging	Het
Glt6d1	ACCC	ACCCC	2: 25,795,167 (GRCm38)		probably null	Het
Gm10717	A	G	9: 3,025,532 (GRCm38)	Y39C	probably benign	Het
Hivep1	C	T	13: 42,158,601 (GRCm38)	T1439I	probably benign	Het
Iqgap2	T	C	13: 95,668,528 (GRCm38)		probably null	Het
Kbtbd11	T	A	8: 15,029,118 (GRCm38)	C572*	probably null	Het
Kif1c	T	C	11: 70,703,771 (GRCm38)	F86L	possibly damaging	Het
Kirrel	C	T	3: 87,089,151 (GRCm38)	M380I	probably null	Het
Ldb1	C	T	19: 46,044,892 (GRCm38)		probably benign	Het
Lrmda	T	A	14: 22,027,331 (GRCm38)		probably benign	Het
Med23	T	C	10: 24,891,120 (GRCm38)	L369S	probably damaging	Het
Mei1	A	G	15: 82,103,204 (GRCm38)	H399R	possibly damaging	Het
Myo1b	G	T	1: 51,776,346 (GRCm38)	H614N	possibly damaging	Het
Neurl1b	C	T	17: 26,414,975 (GRCm38)	T4M	probably damaging	Het
Olfr1095	T	C	2: 86,851,591 (GRCm38)	T36A	probably benign	Het
Olfr129	T	C	17: 38,055,368 (GRCm38)	Y66C	probably damaging	Het
Polg	G	A	7: 79,456,791 (GRCm38)	Q163*	probably null	Het
Pramef8	A	G	4: 143,419,379 (GRCm38)	T473A	probably benign	Het
Sdk2	G	A	11: 113,800,244 (GRCm38)	P1835L	probably damaging	Het
Slc35a5	A	T	16: 45,147,322 (GRCm38)	I138N	probably damaging	Het
Snx31	T	C	15: 36,523,558 (GRCm38)		probably null	Het
Speg	A	T	1: 75,426,782 (GRCm38)	H2590L	probably damaging	Het
Spink4	T	A	4: 40,929,136 (GRCm38)	C54S	probably damaging	Het
Swap70	A	G	7: 110,270,047 (GRCm38)	E349G	probably damaging	Het
Sybu	A	G	15: 44,672,632 (GRCm38)	V766A	possibly damaging	Het
Tnxb	T	A	17: 34,712,964 (GRCm38)	V2157E	possibly damaging	Het
Trak2	A	T	1: 58,946,245 (GRCm38)		probably null	Het
Trav18	C	T	14: 53,831,617 (GRCm38)	R39C	possibly damaging	Het
Uroc1	G	A	6: 90,346,355 (GRCm38)	V352M	probably damaging	Het
Zfp106	A	C	2: 120,534,599 (GRCm38)	I442M	probably benign	Het
Zfp935	G	A	13: 62,455,032 (GRCm38)	Q98*	probably null	Het

Other mutations in Tns3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Tns3	APN	11	8,451,066 (GRCm38)	missense	probably benign	0.42
IGL00822:Tns3	APN	11	8,443,976 (GRCm38)	missense	probably damaging	0.99
IGL01075:Tns3	APN	11	8,478,399 (GRCm38)	missense	probably benign	0.45
IGL01286:Tns3	APN	11	8,492,617 (GRCm38)	missense	probably benign	0.01
IGL01680:Tns3	APN	11	8,548,937 (GRCm38)	missense	probably damaging	1.00
IGL01687:Tns3	APN	11	8,492,798 (GRCm38)	missense	probably damaging	1.00
IGL01734:Tns3	APN	11	8,519,192 (GRCm38)	splice site	probably benign
IGL01844:Tns3	APN	11	8,437,177 (GRCm38)	missense	possibly damaging	0.58
IGL01984:Tns3	APN	11	8,548,992 (GRCm38)	nonsense	probably null
IGL02137:Tns3	APN	11	8,492,578 (GRCm38)	missense	possibly damaging	0.93
IGL02273:Tns3	APN	11	8,434,531 (GRCm38)	missense	probably damaging	1.00
IGL02623:Tns3	APN	11	8,437,141 (GRCm38)	missense	probably damaging	1.00
IGL02697:Tns3	APN	11	8,492,346 (GRCm38)	missense	probably benign	0.00
IGL02829:Tns3	APN	11	8,519,564 (GRCm38)	missense	probably damaging	1.00
ANU74:Tns3	UTSW	11	8,492,149 (GRCm38)	missense	probably benign	0.38
R0020:Tns3	UTSW	11	8,545,227 (GRCm38)	critical splice donor site	probably null
R0064:Tns3	UTSW	11	8,435,856 (GRCm38)	nonsense	probably null
R0064:Tns3	UTSW	11	8,435,856 (GRCm38)	nonsense	probably null
R0370:Tns3	UTSW	11	8,445,730 (GRCm38)	missense	possibly damaging	0.80
R0388:Tns3	UTSW	11	8,445,703 (GRCm38)	missense	probably benign	0.07
R0410:Tns3	UTSW	11	8,435,852 (GRCm38)	missense	probably benign	0.02
R0496:Tns3	UTSW	11	8,547,262 (GRCm38)	splice site	probably benign
R0562:Tns3	UTSW	11	8,493,262 (GRCm38)	missense	possibly damaging	0.93
R0626:Tns3	UTSW	11	8,493,121 (GRCm38)	missense	probably benign	0.04
R0736:Tns3	UTSW	11	8,519,474 (GRCm38)	missense	possibly damaging	0.94
R0893:Tns3	UTSW	11	8,493,302 (GRCm38)	missense	probably damaging	1.00
R1367:Tns3	UTSW	11	8,448,704 (GRCm38)	missense	probably benign	0.01
R1386:Tns3	UTSW	11	8,518,261 (GRCm38)	missense	probably benign	0.02
R1975:Tns3	UTSW	11	8,435,738 (GRCm38)	missense	probably benign	0.04
R2205:Tns3	UTSW	11	8,531,719 (GRCm38)	missense	probably damaging	1.00
R2319:Tns3	UTSW	11	8,541,200 (GRCm38)	missense	probably damaging	1.00
R2830:Tns3	UTSW	11	8,435,870 (GRCm38)	missense	probably damaging	1.00
R3765:Tns3	UTSW	11	8,451,133 (GRCm38)	missense	probably benign	0.00
R3817:Tns3	UTSW	11	8,434,619 (GRCm38)	missense	probably damaging	1.00
R4058:Tns3	UTSW	11	8,492,275 (GRCm38)	missense	probably damaging	1.00
R4599:Tns3	UTSW	11	8,531,747 (GRCm38)	missense	probably damaging	1.00
R4631:Tns3	UTSW	11	8,451,119 (GRCm38)	missense	probably benign	0.30
R4731:Tns3	UTSW	11	8,450,986 (GRCm38)	missense	probably benign	0.28
R4732:Tns3	UTSW	11	8,450,986 (GRCm38)	missense	probably benign	0.28
R4733:Tns3	UTSW	11	8,450,986 (GRCm38)	missense	probably benign	0.28
R5472:Tns3	UTSW	11	8,451,092 (GRCm38)	missense	probably benign
R5749:Tns3	UTSW	11	8,451,177 (GRCm38)	missense	probably benign	0.01
R5807:Tns3	UTSW	11	8,493,211 (GRCm38)	missense	probably damaging	1.00
R5844:Tns3	UTSW	11	8,434,580 (GRCm38)	missense	probably damaging	1.00
R5942:Tns3	UTSW	11	8,435,860 (GRCm38)	missense	probably damaging	1.00
R5982:Tns3	UTSW	11	8,492,245 (GRCm38)	missense	probably damaging	0.99
R6025:Tns3	UTSW	11	8,492,578 (GRCm38)	missense	possibly damaging	0.93
R6266:Tns3	UTSW	11	8,492,987 (GRCm38)	missense	probably damaging	1.00
R6322:Tns3	UTSW	11	8,492,147 (GRCm38)	missense	probably benign	0.01
R6536:Tns3	UTSW	11	8,434,531 (GRCm38)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,549,058 (GRCm38)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,549,057 (GRCm38)	missense	probably damaging	1.00
R6864:Tns3	UTSW	11	8,493,196 (GRCm38)	missense	probably damaging	1.00
R6897:Tns3	UTSW	11	8,531,743 (GRCm38)	missense	probably damaging	1.00
R7108:Tns3	UTSW	11	8,437,251 (GRCm38)	missense	probably benign	0.00
R7443:Tns3	UTSW	11	8,451,442 (GRCm38)	missense	probably benign	0.01
R7459:Tns3	UTSW	11	8,492,793 (GRCm38)	missense	probably benign	0.16
R7474:Tns3	UTSW	11	8,530,894 (GRCm38)	missense	probably damaging	1.00
R7576:Tns3	UTSW	11	8,541,192 (GRCm38)	missense	possibly damaging	0.78
R7979:Tns3	UTSW	11	8,492,701 (GRCm38)	missense	probably benign	0.01
R8055:Tns3	UTSW	11	8,545,343 (GRCm38)	missense	probably damaging	1.00
R8057:Tns3	UTSW	11	8,492,773 (GRCm38)	missense	probably benign
R8077:Tns3	UTSW	11	8,445,667 (GRCm38)	missense	probably damaging	1.00
R8518:Tns3	UTSW	11	8,492,971 (GRCm38)	missense	probably damaging	0.96
R8523:Tns3	UTSW	11	8,448,779 (GRCm38)	missense	probably damaging	1.00
R8790:Tns3	UTSW	11	8,518,273 (GRCm38)	missense	probably damaging	0.99
R9228:Tns3	UTSW	11	8,450,094 (GRCm38)	missense	probably damaging	1.00
R9374:Tns3	UTSW	11	8,492,606 (GRCm38)	missense	probably damaging	1.00
R9476:Tns3	UTSW	11	8,445,702 (GRCm38)	missense	probably damaging	0.99
R9510:Tns3	UTSW	11	8,445,702 (GRCm38)	missense	probably damaging	0.99
R9594:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
R9595:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
R9596:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
R9624:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
R9629:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
T0975:Tns3	UTSW	11	8,451,146 (GRCm38)	missense	probably benign	0.00
T0975:Tns3	UTSW	11	8,549,100 (GRCm38)	start gained	probably benign
T0975:Tns3	UTSW	11	8,479,518 (GRCm38)	missense	probably benign
X0005:Tns3	UTSW	11	8,479,518 (GRCm38)	missense	probably benign
X0005:Tns3	UTSW	11	8,451,224 (GRCm38)	missense	probably benign	0.00
Z1177:Tns3	UTSW	11	8,451,014 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GCCATCAGACAGGAGAGAGTTC -3'
(R):5'- CGACTCAGGTCATTCTACTGCC -3'

Sequencing Primer
(F):5'- CTGGCTGCTCTTGAGACAG -3'
(R):5'- CTCCGCCAGGACTGATAAG -3'

Posted On

2015-01-23