|Institutional Source||Beutler Lab|
|Gene Name||kinesin family member 14|
|Synonyms||N-3 kinesin, D1Ertd367e|
|Is this an essential gene?||Probably essential (E-score: 0.923)|
|Stock #||R0329 (G1)|
|Chromosomal Location||136466343-136531511 bp(+) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||G to C at 136496026 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000144265 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000047817] [ENSMUST00000189413] [ENSMUST00000201676]|
|Coding Region Coverage||
|Validation Efficiency||99% (107/108)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
PHENOTYPE: Mice homozygous for a spontaneous mutation or targeted allele exhibit severe brain malformations, neurological defects and hypomyelination. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Kif14||
(F):5'- GTGTCATCGTAAACTGTGGAGTTAGAGG -3'
(R):5'- GAGGTCTGCCGAAGCATCTGTC -3'
(F):5'- gcatgaaaacagagcactcatac -3'
(R):5'- CCGAAGCATCTGTCCTCTTG -3'