Mutagenetix > Incidental Mutation

Incidental Mutation 'R3406:Mslnl'

259404

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

040624-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3406 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 25746181 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 507 (Y507C)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098] [ENSMUST00000075884]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: Y507C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: Y507C

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000075884

SMART Domains

Protein: ENSMUSP00000075279
Gene: ENSMUSG00000063011

Domain	Start	End	E-Value	Type
Pfam:Mesothelin	1	624	N/A	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.7405

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 93.4%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd18	T	A	3: 40,904,903	M1K	probably null	Het
Bdkrb2	T	C	12: 105,592,496	V332A	possibly damaging	Het
Cdc27	T	C	11: 104,507,200	E778G	probably damaging	Het
Chd4	C	A	6: 125,122,007	T1586K	probably benign	Het
Cnga3	A	G	1: 37,262,065	E622G	probably benign	Het
Dbh	A	G	2: 27,174,965	D396G	possibly damaging	Het
Dhodh	G	A	8: 109,603,475	R86*	probably null	Het
Dpt	A	C	1: 164,796,931	E67A	probably damaging	Het
Eif2ak2	A	G	17: 78,858,639		probably benign	Het
Esp4	T	A	17: 40,602,445	L68M	possibly damaging	Het
Exo1	A	G	1: 175,905,970	K787E	possibly damaging	Het
Fbxo38	T	C	18: 62,514,843	T875A	probably damaging	Het
Gm5346	A	T	8: 43,626,052	C378*	probably null	Het
Gsdma	T	C	11: 98,673,138		probably benign	Het
Hemk1	G	A	9: 107,337,216	Q6*	probably null	Het
Hmcn2	C	T	2: 31,433,272		probably benign	Het
Hook2	A	G	8: 84,993,984		probably benign	Het
Irx3	A	G	8: 91,798,927	S507P	unknown	Het
Kazn	C	A	4: 142,239,195		probably benign	Het
Kcne4	C	T	1: 78,817,971	A112V	possibly damaging	Het
Lamb1	C	T	12: 31,287,529	R372C	probably damaging	Het
Lrrc30	A	G	17: 67,632,180	L135P	probably damaging	Het
Lyst	T	A	13: 13,635,230	M495K	possibly damaging	Het
Mab21l3	C	A	3: 101,823,531	V131F	probably damaging	Het
Mki67	T	A	7: 135,707,475	T416S	probably benign	Het
Mlst8	A	T	17: 24,478,125	M56K	probably benign	Het
Mmp9	A	G	2: 164,949,390	Y160C	probably damaging	Het
Muc6	G	A	7: 141,638,400	S2120F	possibly damaging	Het
Myl12a	A	T	17: 70,994,742	M130K	probably benign	Het
Ncdn	C	T	4: 126,748,595	R423Q	probably benign	Het
Ncf2	A	G	1: 152,825,947		probably benign	Het
Nek8	G	T	11: 78,170,746	S319*	probably null	Het
Olfr553	T	C	7: 102,614,786	M68V	possibly damaging	Het
Olfr740	G	A	14: 50,453,196	C48Y	probably benign	Het
Pcdh17	T	A	14: 84,446,622	D176E	probably damaging	Het
Pcdhb15	C	A	18: 37,475,389	A558E	probably benign	Het
Plrg1	T	A	3: 83,071,219	W431R	probably damaging	Het
Rbfox3	T	A	11: 118,496,457	Q277L	possibly damaging	Het
Rpgrip1	G	A	14: 52,145,209	D600N	possibly damaging	Het
Siah3	A	G	14: 75,525,981	D224G	probably damaging	Het
Slc22a6	T	C	19: 8,621,311	L244P	probably damaging	Het
Stap2	A	T	17: 55,997,511	W374R	probably benign	Het
Tbck	T	A	3: 132,727,084	N418K	probably benign	Het
Tcp11x2	T	C	X: 135,654,984	N474S	probably damaging	Het
Tenm3	A	C	8: 48,228,555	V2680G	probably damaging	Het
Thada	A	T	17: 84,230,785		probably benign	Het
Tlr6	C	T	5: 64,953,429	V712M	probably damaging	Het
Tmem28	A	G	X: 99,845,503	I325V	probably benign	Het
Uvssa	G	T	5: 33,389,818	G243C	probably damaging	Het
Vwa8	T	A	14: 79,164,220		probably benign	Het
Znrf2	A	T	6: 54,884,791	N229I	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- TATACAGAGGGCAAGCACTTTAAAG -3'
(R):5'- TGTCCATGGAGATGTTGGCC -3'

Sequencing Primer
(F):5'- AGGATACACCCAGTGGTCC -3'
(R):5'- CCTGTGCCAGGTATTGAAGTTCC -3'

Posted On

2015-01-23