Mutagenetix > Incidental Mutation

Incidental Mutation 'R3715:Zfp236'

259881

Institutional Source

Beutler Lab

Gene Symbol

Zfp236

Ensembl Gene

ENSMUSG00000041258

Gene Name

zinc finger protein 236

Synonyms

LOC240456

MMRRC Submission

040708-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R3715 (G1)

Quality Score

214

Status

Validated

Chromosome

Chromosomal Location

82611718-82711008 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 82651095 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000138179 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171071] [ENSMUST00000182122] [ENSMUST00000183048]

AlphaFold

S4R299

Predicted Effect

probably benign
Transcript: ENSMUST00000171071

SMART Domains

Protein: ENSMUSP00000130004
Gene: ENSMUSG00000041258

Domain	Start	End	E-Value	Type
ZnF_C2H2	39	61	2.24e-3	SMART
ZnF_C2H2	68	90	2.29e0	SMART
ZnF_C2H2	95	117	1.26e-2	SMART
ZnF_C2H2	123	145	6.67e-2	SMART
ZnF_C2H2	155	177	6.42e-4	SMART
ZnF_C2H2	199	221	1.75e-5	SMART
ZnF_C2H2	227	249	1.52e-5	SMART
ZnF_C2H2	255	278	8.94e-3	SMART
low complexity region	290	309	N/A	INTRINSIC
low complexity region	403	426	N/A	INTRINSIC
ZnF_C2H2	436	458	1.98e-4	SMART
ZnF_C2H2	464	486	9.58e-3	SMART
ZnF_C2H2	492	514	6.42e-4	SMART
ZnF_C2H2	520	542	1.18e-2	SMART
low complexity region	592	605	N/A	INTRINSIC
ZnF_C2H2	611	633	1.62e0	SMART
ZnF_C2H2	639	661	5.21e-4	SMART
ZnF_C2H2	667	689	6.78e-3	SMART
ZnF_C2H2	695	717	7.37e-4	SMART
low complexity region	720	733	N/A	INTRINSIC
ZnF_C2H2	922	944	5.21e-4	SMART
ZnF_C2H2	950	972	1.04e-3	SMART
ZnF_C2H2	978	1000	8.6e-5	SMART
ZnF_C2H2	1006	1028	2.75e-3	SMART
low complexity region	1030	1039	N/A	INTRINSIC
ZnF_C2H2	1122	1144	7.78e-3	SMART
ZnF_C2H2	1150	1172	3.63e-3	SMART
ZnF_C2H2	1178	1200	6.88e-4	SMART
ZnF_C2H2	1206	1228	5.42e-2	SMART
low complexity region	1243	1258	N/A	INTRINSIC
low complexity region	1462	1477	N/A	INTRINSIC
ZnF_C2H2	1612	1635	7.15e-2	SMART
ZnF_C2H2	1641	1663	2.91e-2	SMART
ZnF_C2H2	1677	1699	7.26e-3	SMART
ZnF_C2H2	1705	1727	1.84e-4	SMART
ZnF_C2H2	1733	1756	2.95e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000182122

SMART Domains

Protein: ENSMUSP00000138557
Gene: ENSMUSG00000041258

Domain	Start	End	E-Value	Type
ZnF_C2H2	39	61	2.24e-3	SMART
ZnF_C2H2	68	90	2.29e0	SMART
ZnF_C2H2	95	117	1.26e-2	SMART
ZnF_C2H2	123	145	6.67e-2	SMART
ZnF_C2H2	155	177	6.42e-4	SMART
ZnF_C2H2	199	221	1.75e-5	SMART
ZnF_C2H2	227	249	1.52e-5	SMART
ZnF_C2H2	255	278	8.94e-3	SMART
ZnF_C2H2	287	310	9.58e-3	SMART
low complexity region	338	357	N/A	INTRINSIC
low complexity region	451	474	N/A	INTRINSIC
ZnF_C2H2	484	506	1.98e-4	SMART
ZnF_C2H2	512	534	9.58e-3	SMART
ZnF_C2H2	540	562	6.42e-4	SMART
ZnF_C2H2	568	590	1.18e-2	SMART
low complexity region	640	653	N/A	INTRINSIC
ZnF_C2H2	659	681	1.62e0	SMART
ZnF_C2H2	687	709	5.21e-4	SMART
ZnF_C2H2	715	737	6.78e-3	SMART
ZnF_C2H2	743	765	7.37e-4	SMART
low complexity region	768	781	N/A	INTRINSIC
ZnF_C2H2	970	992	5.21e-4	SMART
ZnF_C2H2	998	1020	1.04e-3	SMART
ZnF_C2H2	1026	1048	8.6e-5	SMART
ZnF_C2H2	1054	1076	2.75e-3	SMART
low complexity region	1078	1087	N/A	INTRINSIC
ZnF_C2H2	1170	1192	7.78e-3	SMART
ZnF_C2H2	1198	1220	3.63e-3	SMART
ZnF_C2H2	1226	1248	6.88e-4	SMART
ZnF_C2H2	1254	1276	5.42e-2	SMART
low complexity region	1291	1306	N/A	INTRINSIC
low complexity region	1510	1525	N/A	INTRINSIC
ZnF_C2H2	1660	1683	7.15e-2	SMART
ZnF_C2H2	1689	1711	2.91e-2	SMART
ZnF_C2H2	1725	1747	7.26e-3	SMART
ZnF_C2H2	1753	1775	1.84e-4	SMART
ZnF_C2H2	1781	1804	2.95e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000183048

SMART Domains

Protein: ENSMUSP00000138179
Gene: ENSMUSG00000041258

Domain	Start	End	E-Value	Type
ZnF_C2H2	39	61	2.24e-3	SMART
ZnF_C2H2	68	90	2.29e0	SMART
ZnF_C2H2	95	117	1.26e-2	SMART
ZnF_C2H2	123	145	6.67e-2	SMART
ZnF_C2H2	155	177	6.42e-4	SMART
ZnF_C2H2	199	221	1.75e-5	SMART
ZnF_C2H2	227	249	1.52e-5	SMART
ZnF_C2H2	255	278	8.94e-3	SMART
ZnF_C2H2	287	310	9.58e-3	SMART
low complexity region	338	357	N/A	INTRINSIC
low complexity region	454	467	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.2%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310079G19Rik	T	C	16: 88,424,081 (GRCm39)	M137V	probably benign	Het
9930111J21Rik1	T	C	11: 48,838,803 (GRCm39)	T595A	possibly damaging	Het
Aaas	T	C	15: 102,248,771 (GRCm39)	I236V	probably benign	Het
Abcd4	C	T	12: 84,658,533 (GRCm39)	M223I	probably benign	Het
Adamtsl1	T	A	4: 86,135,213 (GRCm39)	I246N	probably benign	Het
Ak9	A	G	10: 41,233,508 (GRCm39)	D582G	probably damaging	Het
Aqr	A	G	2: 113,949,150 (GRCm39)		probably benign	Het
Bmal2	A	G	6: 146,724,187 (GRCm39)	K360E	probably damaging	Het
Cacna2d3	T	C	14: 29,068,880 (GRCm39)	I282M	probably damaging	Het
Cers3	G	T	7: 66,435,823 (GRCm39)	A261S	probably benign	Het
Dexi	A	T	16: 10,360,553 (GRCm39)	M1K	probably null	Het
Dlat	A	G	9: 50,549,354 (GRCm39)	V510A	probably damaging	Het
Eaf1	T	C	14: 31,224,402 (GRCm39)	I173T	possibly damaging	Het
Elavl3	G	T	9: 21,929,895 (GRCm39)	D336E	probably benign	Het
Epm2a	A	G	10: 11,219,420 (GRCm39)	Y69C	probably benign	Het
Fam168a	A	G	7: 100,473,432 (GRCm39)	N107S	probably damaging	Het
Fam43b	G	C	4: 138,122,409 (GRCm39)	R304G	probably benign	Het
Fras1	T	C	5: 96,793,829 (GRCm39)		probably null	Het
Fscn3	C	T	6: 28,428,091 (GRCm39)	T26I	possibly damaging	Het
Glt8d2	C	A	10: 82,488,571 (GRCm39)	A300S	probably benign	Het
Hcn4	G	A	9: 58,751,319 (GRCm39)	R315H	unknown	Het
Lipk	A	G	19: 34,017,829 (GRCm39)	N289S	probably damaging	Het
Lyg1	G	T	1: 37,989,759 (GRCm39)	R43S	probably damaging	Het
Marchf6	A	G	15: 31,465,405 (GRCm39)	L833P	probably benign	Het
Mc4r	T	A	18: 66,992,892 (GRCm39)	N74Y	probably damaging	Het
Med17	G	A	9: 15,175,062 (GRCm39)		probably benign	Het
Mink1	G	T	11: 70,499,776 (GRCm39)	R773L	possibly damaging	Het
Myo15a	A	T	11: 60,370,057 (GRCm39)	E939V	possibly damaging	Het
Ndst4	A	T	3: 125,355,154 (GRCm39)	H354L	possibly damaging	Het
Or2t26	T	C	11: 49,039,642 (GRCm39)	L186P	probably damaging	Het
Or4c15	A	G	2: 88,759,757 (GRCm39)	W301R	probably benign	Het
Or4p22	C	A	2: 88,317,787 (GRCm39)	T237N	probably damaging	Het
Otof	T	A	5: 30,534,215 (GRCm39)	K1397*	probably null	Het
Rangap1	C	A	15: 81,594,661 (GRCm39)	E389D	probably benign	Het
Rbfox2	A	G	15: 76,983,451 (GRCm39)	I270T	probably damaging	Het
Rnf217	G	T	10: 31,410,728 (GRCm39)	C322*	probably null	Het
Sbk1	A	G	7: 125,889,183 (GRCm39)	T50A	probably benign	Het
Shmt1	T	C	11: 60,688,402 (GRCm39)	T248A	probably damaging	Het
Smim29	G	A	17: 27,785,043 (GRCm39)		probably benign	Het
Sox30	C	T	11: 45,875,619 (GRCm39)	T457I	probably damaging	Het
Stox2	T	A	8: 47,866,187 (GRCm39)	I52F	possibly damaging	Het
Syncrip	A	T	9: 88,361,738 (GRCm39)		probably benign	Het
Tars3	G	A	7: 65,338,700 (GRCm39)		probably null	Het
Tdrd12	T	C	7: 35,204,405 (GRCm39)	E235G	probably benign	Het
Tmem82	A	T	4: 141,344,945 (GRCm39)		probably null	Het
Tro	T	C	X: 149,437,230 (GRCm39)	T476A	probably damaging	Het
Ttn	G	A	2: 76,561,363 (GRCm39)	P27302S	probably damaging	Het
Ttn	C	T	2: 76,571,610 (GRCm39)	V26428I	probably damaging	Het
Usp53	T	C	3: 122,742,968 (GRCm39)	E656G	probably benign	Het
Vmn2r100	A	G	17: 19,752,272 (GRCm39)	R772G	probably damaging	Het
Xkr5	T	C	8: 18,984,474 (GRCm39)	E190G	probably benign	Het
Zfr2	T	G	10: 81,081,913 (GRCm39)	V493G	probably benign	Het
Zp2	A	T	7: 119,741,057 (GRCm39)	S156T	possibly damaging	Het
Zswim5	A	G	4: 116,819,755 (GRCm39)	T387A	probably benign	Het

Other mutations in Zfp236

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01016:Zfp236	APN	18	82,686,815 (GRCm39)	missense	probably benign	0.44
IGL01760:Zfp236	APN	18	82,639,547 (GRCm39)	missense	probably damaging	1.00
IGL01923:Zfp236	APN	18	82,700,344 (GRCm39)	missense	probably damaging	0.98
IGL01934:Zfp236	APN	18	82,651,245 (GRCm39)	missense	probably damaging	0.99
IGL01949:Zfp236	APN	18	82,642,521 (GRCm39)	missense	probably damaging	1.00
IGL02063:Zfp236	APN	18	82,676,276 (GRCm39)	missense	probably benign
IGL02496:Zfp236	APN	18	82,648,117 (GRCm39)	missense	probably damaging	1.00
IGL02513:Zfp236	APN	18	82,648,239 (GRCm39)	missense	probably damaging	1.00
IGL02626:Zfp236	APN	18	82,676,120 (GRCm39)	splice site	probably benign
IGL02880:Zfp236	APN	18	82,642,584 (GRCm39)	missense	probably benign	0.15
IGL03156:Zfp236	APN	18	82,698,827 (GRCm39)	missense	probably damaging	1.00
IGL03261:Zfp236	APN	18	82,648,733 (GRCm39)	missense	possibly damaging	0.93
R0047:Zfp236	UTSW	18	82,698,817 (GRCm39)	missense	probably damaging	1.00
R0052:Zfp236	UTSW	18	82,657,457 (GRCm39)	missense	probably damaging	1.00
R0194:Zfp236	UTSW	18	82,675,112 (GRCm39)	missense	probably damaging	1.00
R0207:Zfp236	UTSW	18	82,658,352 (GRCm39)	missense	probably damaging	1.00
R0234:Zfp236	UTSW	18	82,648,119 (GRCm39)	missense	probably damaging	1.00
R0234:Zfp236	UTSW	18	82,648,119 (GRCm39)	missense	probably damaging	1.00
R0302:Zfp236	UTSW	18	82,676,213 (GRCm39)	missense	probably damaging	0.99
R0730:Zfp236	UTSW	18	82,658,369 (GRCm39)	splice site	probably benign
R0755:Zfp236	UTSW	18	82,638,457 (GRCm39)	missense	probably damaging	1.00
R1202:Zfp236	UTSW	18	82,646,291 (GRCm39)	missense	probably benign	0.00
R1449:Zfp236	UTSW	18	82,664,130 (GRCm39)	missense	probably damaging	1.00
R1550:Zfp236	UTSW	18	82,692,549 (GRCm39)	missense	possibly damaging	0.81
R1785:Zfp236	UTSW	18	82,639,429 (GRCm39)	missense	probably benign	0.08
R1786:Zfp236	UTSW	18	82,639,429 (GRCm39)	missense	probably benign	0.08
R2042:Zfp236	UTSW	18	82,651,234 (GRCm39)	missense	probably damaging	1.00
R2132:Zfp236	UTSW	18	82,639,429 (GRCm39)	missense	probably benign	0.08
R2133:Zfp236	UTSW	18	82,639,429 (GRCm39)	missense	probably benign	0.08
R2247:Zfp236	UTSW	18	82,622,423 (GRCm39)	missense	possibly damaging	0.82
R2484:Zfp236	UTSW	18	82,686,762 (GRCm39)	missense	probably benign	0.05
R4003:Zfp236	UTSW	18	82,698,870 (GRCm39)	nonsense	probably null
R4031:Zfp236	UTSW	18	82,642,590 (GRCm39)	missense	probably damaging	1.00
R4482:Zfp236	UTSW	18	82,662,346 (GRCm39)	missense	probably benign	0.04
R4492:Zfp236	UTSW	18	82,648,125 (GRCm39)	missense	probably damaging	1.00
R4502:Zfp236	UTSW	18	82,655,079 (GRCm39)	missense	probably benign	0.13
R4561:Zfp236	UTSW	18	82,638,531 (GRCm39)	missense	probably damaging	1.00
R4649:Zfp236	UTSW	18	82,615,784 (GRCm39)	missense	probably damaging	1.00
R4902:Zfp236	UTSW	18	82,627,543 (GRCm39)	missense	possibly damaging	0.89
R5064:Zfp236	UTSW	18	82,709,701 (GRCm39)	critical splice donor site	probably null
R5084:Zfp236	UTSW	18	82,627,556 (GRCm39)	missense	probably damaging	1.00
R5090:Zfp236	UTSW	18	82,637,006 (GRCm39)	missense	probably benign	0.08
R5191:Zfp236	UTSW	18	82,639,548 (GRCm39)	missense	probably damaging	1.00
R5264:Zfp236	UTSW	18	82,676,198 (GRCm39)	missense	probably damaging	0.99
R5264:Zfp236	UTSW	18	82,648,219 (GRCm39)	missense	probably damaging	1.00
R5339:Zfp236	UTSW	18	82,642,491 (GRCm39)	missense	probably damaging	0.99
R5375:Zfp236	UTSW	18	82,615,813 (GRCm39)	missense	possibly damaging	0.93
R5445:Zfp236	UTSW	18	82,700,281 (GRCm39)	missense	probably benign	0.02
R5513:Zfp236	UTSW	18	82,676,147 (GRCm39)	missense	probably damaging	0.97
R5527:Zfp236	UTSW	18	82,676,159 (GRCm39)	missense	possibly damaging	0.51
R5628:Zfp236	UTSW	18	82,675,247 (GRCm39)	missense	probably damaging	1.00
R5758:Zfp236	UTSW	18	82,689,834 (GRCm39)	missense	probably damaging	1.00
R5890:Zfp236	UTSW	18	82,658,276 (GRCm39)	missense	possibly damaging	0.87
R6137:Zfp236	UTSW	18	82,689,919 (GRCm39)	missense	possibly damaging	0.89
R6193:Zfp236	UTSW	18	82,622,372 (GRCm39)	missense	probably damaging	1.00
R6198:Zfp236	UTSW	18	82,675,278 (GRCm39)	missense	probably damaging	1.00
R6239:Zfp236	UTSW	18	82,675,229 (GRCm39)	missense	possibly damaging	0.53
R6705:Zfp236	UTSW	18	82,651,862 (GRCm39)	missense	probably damaging	0.97
R6948:Zfp236	UTSW	18	82,662,187 (GRCm39)	missense	possibly damaging	0.94
R6989:Zfp236	UTSW	18	82,646,488 (GRCm39)	missense	probably damaging	1.00
R7002:Zfp236	UTSW	18	82,709,701 (GRCm39)	critical splice donor site	probably null
R7113:Zfp236	UTSW	18	82,638,462 (GRCm39)	missense	possibly damaging	0.82
R7261:Zfp236	UTSW	18	82,627,470 (GRCm39)	missense	possibly damaging	0.86
R7363:Zfp236	UTSW	18	82,639,456 (GRCm39)	missense	probably damaging	1.00
R7447:Zfp236	UTSW	18	82,651,815 (GRCm39)	missense	probably damaging	1.00
R7564:Zfp236	UTSW	18	82,662,366 (GRCm39)	nonsense	probably null
R7731:Zfp236	UTSW	18	82,698,798 (GRCm39)	missense	probably benign	0.27
R7857:Zfp236	UTSW	18	82,686,726 (GRCm39)	nonsense	probably null
R7860:Zfp236	UTSW	18	82,692,481 (GRCm39)	nonsense	probably null
R7904:Zfp236	UTSW	18	82,627,507 (GRCm39)	missense	possibly damaging	0.90
R7948:Zfp236	UTSW	18	82,642,540 (GRCm39)	missense	probably damaging	1.00
R7995:Zfp236	UTSW	18	82,657,461 (GRCm39)	missense	probably damaging	1.00
R8153:Zfp236	UTSW	18	82,648,152 (GRCm39)	missense	probably damaging	1.00
R8435:Zfp236	UTSW	18	82,658,366 (GRCm39)	missense	probably damaging	1.00
R8560:Zfp236	UTSW	18	82,664,340 (GRCm39)	missense	probably damaging	1.00
R8878:Zfp236	UTSW	18	82,617,122 (GRCm39)	missense	probably damaging	1.00
R8916:Zfp236	UTSW	18	82,664,351 (GRCm39)	missense	probably damaging	1.00
R9046:Zfp236	UTSW	18	82,637,042 (GRCm39)	missense	possibly damaging	0.89
R9076:Zfp236	UTSW	18	82,638,469 (GRCm39)	missense	possibly damaging	0.77
R9243:Zfp236	UTSW	18	82,662,050 (GRCm39)	intron	probably benign
R9594:Zfp236	UTSW	18	82,664,238 (GRCm39)	missense	probably damaging	1.00
R9642:Zfp236	UTSW	18	82,622,384 (GRCm39)	missense	probably benign	0.00
R9707:Zfp236	UTSW	18	82,664,328 (GRCm39)	missense	probably damaging	1.00
R9748:Zfp236	UTSW	18	82,637,008 (GRCm39)	missense	possibly damaging	0.89

Predicted Primers

PCR Primer
(F):5'- TGGTGGCTCTCAGTCAAAATGG -3'
(R):5'- GTCACTAACACTTGCTGCATC -3'

Sequencing Primer
(F):5'- TGGCTCTCAGTCAAAATGGCAAAG -3'
(R):5'- AACACTTGCTGCATCCTGAC -3'

Posted On

2015-01-23