Mutagenetix > Incidental Mutation

Incidental Mutation 'R2890:Vmn2r66'

260061

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r66

Ensembl Gene

ENSMUSG00000094950

Gene Name

vomeronasal 2, receptor 66

Synonyms

F830104D24Rik

MMRRC Submission

040478-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R2890 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

84643853-84661228 bp(-) (GRCm39)

Type of Mutation

splice site (5 bp from exon)

DNA Base Change (assembly)

C to T at 84661027 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000122645 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000124773]

AlphaFold

A0A3B2W842

Predicted Effect

probably null
Transcript: ENSMUST00000124773

SMART Domains

Protein: ENSMUSP00000122645
Gene: ENSMUSG00000094950

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	463	5e-31	PFAM
Pfam:NCD3G	507	559	6e-21	PFAM
Pfam:7tm_3	589	827	3.8e-52	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930480E11Rik	A	T	X: 77,414,288 (GRCm39)	I338F	probably damaging	Het
A1cf	A	G	19: 31,895,417 (GRCm39)	I167V	probably benign	Het
Adamts20	C	T	15: 94,228,459 (GRCm39)	R996H	probably benign	Het
Amdhd1	T	C	10: 93,363,126 (GRCm39)	K321E	probably benign	Het
Baz1a	T	C	12: 54,945,302 (GRCm39)	H1351R	probably benign	Het
Cep63	T	C	9: 102,496,026 (GRCm39)	D127G	probably damaging	Het
Chrm5	T	C	2: 112,310,048 (GRCm39)	Y356C	probably benign	Het
Dock4	A	T	12: 40,673,800 (GRCm39)		probably null	Het
Dync1h1	T	C	12: 110,583,325 (GRCm39)	L474P	probably damaging	Het
Fzd7	C	T	1: 59,523,593 (GRCm39)	A492V	probably benign	Het
Greb1	A	G	12: 16,754,479 (GRCm39)	L876P	probably damaging	Het
Grik4	T	C	9: 42,582,515 (GRCm39)	T144A	probably damaging	Het
Gusb	T	C	5: 130,029,343 (GRCm39)	H146R	probably damaging	Het
Hspg2	T	C	4: 137,276,885 (GRCm39)	V2835A	probably damaging	Het
Lamp1	T	A	8: 13,223,891 (GRCm39)	L341H	probably damaging	Het
Lcn10	T	C	2: 25,573,642 (GRCm39)	L73P	probably damaging	Het
Nostrin	A	G	2: 69,011,249 (GRCm39)	T347A	probably benign	Het
Or4k41	A	T	2: 111,279,634 (GRCm39)	I50F	probably benign	Het
Pcdhb9	G	A	18: 37,536,379 (GRCm39)	C791Y	probably benign	Het
Phex	T	C	X: 156,093,954 (GRCm39)	I439V	probably benign	Het
Rbsn	C	T	6: 92,184,104 (GRCm39)	V70M	possibly damaging	Het
Slc25a1	A	T	16: 17,743,963 (GRCm39)	L251Q	probably damaging	Het
Slc35a5	A	G	16: 44,971,923 (GRCm39)	C114R	probably damaging	Het
Srek1ip1	A	T	13: 104,970,758 (GRCm39)	I70L	probably benign	Het
Taf4b	T	C	18: 14,937,849 (GRCm39)	S278P	probably damaging	Het
Ttn	T	C	2: 76,726,498 (GRCm39)		probably benign	Het
Vmn2r13	T	C	5: 109,339,840 (GRCm39)	D45G	possibly damaging	Het
Ylpm1	G	T	12: 85,076,587 (GRCm39)	R646L	probably damaging	Het
Zfp51	C	G	17: 21,684,118 (GRCm39)	C244W	probably damaging	Het
Zfp619	C	A	7: 39,184,393 (GRCm39)	T141K	probably benign	Het

Other mutations in Vmn2r66

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00321:Vmn2r66	APN	7	84,656,299 (GRCm39)	missense	probably benign
IGL01562:Vmn2r66	APN	7	84,656,495 (GRCm39)	missense	probably benign	0.03
IGL01689:Vmn2r66	APN	7	84,657,033 (GRCm39)	missense	probably damaging	1.00
IGL02237:Vmn2r66	APN	7	84,643,908 (GRCm39)	missense	probably benign
IGL02415:Vmn2r66	APN	7	84,656,020 (GRCm39)	missense	probably damaging	0.97
IGL02439:Vmn2r66	APN	7	84,654,455 (GRCm39)	splice site	probably benign
IGL02545:Vmn2r66	APN	7	84,655,798 (GRCm39)	missense	possibly damaging	0.50
IGL02708:Vmn2r66	APN	7	84,655,796 (GRCm39)	missense	probably benign	0.00
IGL02794:Vmn2r66	APN	7	84,644,623 (GRCm39)	missense	probably benign	0.00
IGL02885:Vmn2r66	APN	7	84,644,723 (GRCm39)	missense	probably benign	0.00
IGL02975:Vmn2r66	APN	7	84,656,182 (GRCm39)	missense	probably damaging	0.98
IGL03027:Vmn2r66	APN	7	84,644,777 (GRCm39)	splice site	probably benign
IGL03081:Vmn2r66	APN	7	84,657,138 (GRCm39)	missense	probably benign
PIT4131001:Vmn2r66	UTSW	7	84,644,301 (GRCm39)	missense	probably damaging	1.00
R0098:Vmn2r66	UTSW	7	84,654,965 (GRCm39)	missense	probably damaging	1.00
R0504:Vmn2r66	UTSW	7	84,656,023 (GRCm39)	missense	probably damaging	0.99
R0557:Vmn2r66	UTSW	7	84,643,972 (GRCm39)	missense	probably damaging	1.00
R0617:Vmn2r66	UTSW	7	84,644,484 (GRCm39)	missense	probably benign	0.02
R0883:Vmn2r66	UTSW	7	84,657,070 (GRCm39)	missense	probably benign
R1159:Vmn2r66	UTSW	7	84,644,613 (GRCm39)	missense	probably benign	0.44
R1168:Vmn2r66	UTSW	7	84,656,062 (GRCm39)	missense	possibly damaging	0.46
R1172:Vmn2r66	UTSW	7	84,654,799 (GRCm39)	missense	probably benign	0.04
R1175:Vmn2r66	UTSW	7	84,654,799 (GRCm39)	missense	probably benign	0.04
R1538:Vmn2r66	UTSW	7	84,644,166 (GRCm39)	missense	possibly damaging	0.84
R1658:Vmn2r66	UTSW	7	84,656,955 (GRCm39)	missense	probably benign	0.07
R1937:Vmn2r66	UTSW	7	84,644,344 (GRCm39)	missense	probably damaging	0.99
R1989:Vmn2r66	UTSW	7	84,661,201 (GRCm39)	missense	probably benign	0.01
R2698:Vmn2r66	UTSW	7	84,644,607 (GRCm39)	missense	probably damaging	1.00
R3686:Vmn2r66	UTSW	7	84,644,397 (GRCm39)	missense	probably damaging	0.96
R4152:Vmn2r66	UTSW	7	84,654,800 (GRCm39)	missense	probably benign	0.08
R4500:Vmn2r66	UTSW	7	84,657,162 (GRCm39)	missense	probably damaging	1.00
R4618:Vmn2r66	UTSW	7	84,644,296 (GRCm39)	missense	possibly damaging	0.62
R4656:Vmn2r66	UTSW	7	84,661,204 (GRCm39)	missense	possibly damaging	0.87
R4668:Vmn2r66	UTSW	7	84,643,905 (GRCm39)	missense	probably damaging	1.00
R4942:Vmn2r66	UTSW	7	84,656,980 (GRCm39)	missense	probably damaging	1.00
R5163:Vmn2r66	UTSW	7	84,656,017 (GRCm39)	missense	probably benign	0.01
R5223:Vmn2r66	UTSW	7	84,657,093 (GRCm39)	missense	probably benign
R5377:Vmn2r66	UTSW	7	84,656,026 (GRCm39)	missense	probably damaging	0.99
R5512:Vmn2r66	UTSW	7	84,657,149 (GRCm39)	missense	probably damaging	1.00
R5611:Vmn2r66	UTSW	7	84,654,951 (GRCm39)	nonsense	probably null
R5749:Vmn2r66	UTSW	7	84,655,979 (GRCm39)	nonsense	probably null
R6131:Vmn2r66	UTSW	7	84,644,224 (GRCm39)	missense	probably damaging	1.00
R6183:Vmn2r66	UTSW	7	84,644,766 (GRCm39)	missense	possibly damaging	0.81
R6509:Vmn2r66	UTSW	7	84,656,054 (GRCm39)	missense	probably benign	0.12
R6930:Vmn2r66	UTSW	7	84,661,216 (GRCm39)	missense	possibly damaging	0.80
R6992:Vmn2r66	UTSW	7	84,654,436 (GRCm39)	missense	possibly damaging	0.90
R7015:Vmn2r66	UTSW	7	84,644,766 (GRCm39)	missense	possibly damaging	0.81
R7302:Vmn2r66	UTSW	7	84,654,423 (GRCm39)	missense	probably benign	0.00
R7516:Vmn2r66	UTSW	7	84,661,176 (GRCm39)	missense	possibly damaging	0.51
R7763:Vmn2r66	UTSW	7	84,654,909 (GRCm39)	missense	probably benign	0.01
R7814:Vmn2r66	UTSW	7	84,656,472 (GRCm39)	missense	probably benign	0.02
R8077:Vmn2r66	UTSW	7	84,656,093 (GRCm39)	missense	probably benign
R8307:Vmn2r66	UTSW	7	84,656,270 (GRCm39)	missense	probably benign
R8315:Vmn2r66	UTSW	7	84,643,932 (GRCm39)	missense	possibly damaging	0.90
R8490:Vmn2r66	UTSW	7	84,654,794 (GRCm39)	critical splice donor site	probably null
R8511:Vmn2r66	UTSW	7	84,656,026 (GRCm39)	missense	probably damaging	0.99
R8781:Vmn2r66	UTSW	7	84,644,355 (GRCm39)	nonsense	probably null
R8812:Vmn2r66	UTSW	7	84,654,893 (GRCm39)	missense	probably damaging	0.99
R9203:Vmn2r66	UTSW	7	84,654,950 (GRCm39)	missense	probably benign	0.01
R9277:Vmn2r66	UTSW	7	84,661,164 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GGTCATTCATTCAGCATCTGCC -3'
(R):5'- ATGTATGTGCTGCATTTTACTCAGG -3'

Sequencing Primer
(F):5'- CAGCATCTGCCTAGAAATTGTGACTC -3'
(R):5'- TGCTGATGTGAAAAAGATGTTCAC -3'

Posted On

2015-01-23