Mutagenetix > Incidental Mutation

Incidental Mutation 'R2877:Mslnl'

260640

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

040465-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2877 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

96% (66/69)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	C	5: 109,738,945		probably benign	Het
Abca13	C	T	11: 9,291,889	L1251F	possibly damaging	Het
Accsl	A	T	2: 93,859,410	M384K	probably damaging	Het
Adssl1	A	C	12: 112,634,189	K197N	probably damaging	Het
Alg11	A	G	8: 22,065,358	N170D	possibly damaging	Het
Ambn	T	C	5: 88,460,700		probably benign	Het
Anapc7	T	C	5: 122,428,156	Y43H	probably benign	Het
Anxa10	T	A	8: 62,060,339	I255F	probably damaging	Het
Atg2b	A	T	12: 105,664,009	Y374*	probably null	Het
Axl	T	A	7: 25,766,524	M563L	probably damaging	Het
Carmil2	A	G	8: 105,695,423	E1108G	probably damaging	Het
Casp1	T	C	9: 5,303,110	M188T	probably damaging	Het
Chd3	A	T	11: 69,361,172	C87*	probably null	Het
Col6a3	G	A	1: 90,775,599	T2475I	unknown	Het
Creb3l4	A	T	3: 90,242,308	S83R	probably damaging	Het
Cyp2a4	G	A	7: 26,312,187	E278K	possibly damaging	Het
Dync1li2	A	C	8: 104,429,415	Y265D	probably damaging	Het
Eif3f	G	A	7: 108,934,812		probably null	Het
Eipr1	C	T	12: 28,760,092	T22I	possibly damaging	Het
Fbxo48	A	G	11: 16,953,382	K3E	possibly damaging	Het
Fbxw13	A	G	9: 109,181,466	F368S	probably damaging	Het
Fbxw19	A	T	9: 109,485,970	W175R	probably damaging	Het
Fibcd1	G	A	2: 31,838,666	P60S	probably benign	Het
Foxa3	A	T	7: 19,014,880	M107K	probably benign	Het
Foxj2	G	A	6: 122,842,832	D560N	probably damaging	Het
Gfm2	A	G	13: 97,153,249	R181G	possibly damaging	Het
Gpr25	C	T	1: 136,260,815	G20D	possibly damaging	Het
Grin1	A	G	2: 25,297,629	V594A	probably damaging	Het
Itpripl1	A	G	2: 127,141,614	V196A	probably benign	Het
Kcns1	A	G	2: 164,164,762	I427T	probably damaging	Het
Kiz	T	C	2: 146,889,556	V322A	possibly damaging	Het
Muc19	T	A	15: 91,893,006		noncoding transcript	Het
Naa16	A	G	14: 79,343,298	M592T	probably benign	Het
Nat6	A	T	9: 107,583,168	E87D	possibly damaging	Het
Ncapd3	T	A	9: 27,044,487		probably null	Het
Nebl	C	A	2: 17,434,929	D178Y	probably damaging	Het
Nedd1	T	A	10: 92,714,126	N99I	possibly damaging	Het
Nuak1	T	C	10: 84,375,345	D293G	possibly damaging	Het
Olfr1036	T	C	2: 86,075,331	M197T	possibly damaging	Het
Olfr1487	A	T	19: 13,619,632	I157F	probably damaging	Het
Palb2	A	G	7: 122,114,429	V877A	probably damaging	Het
Rassf6	C	T	5: 90,606,805	V205I	probably damaging	Het
Rbak	A	G	5: 143,174,105	Y398H	probably damaging	Het
Rcbtb1	A	G	14: 59,210,592		probably benign	Het
Robo4	CGG	CG	9: 37,411,490		probably null	Het
Smpdl3a	C	A	10: 57,809,085	T317K	probably damaging	Het
Speer4e	C	T	5: 14,937,116	V92M	probably damaging	Het
Syne2	A	G	12: 76,000,831	I4009V	probably benign	Het
Tarbp1	G	A	8: 126,427,832	L1474F	probably damaging	Het
Tchh	A	G	3: 93,444,228	E325G	unknown	Het
Trpc4	T	C	3: 54,291,340	S562P	probably damaging	Het
Ttn	T	C	2: 76,737,065	D27828G	probably damaging	Het
Ulk4	T	C	9: 121,260,039	D258G	probably benign	Het
Vmn1r215	A	G	13: 23,076,561	H257R	probably benign	Het
Vmn2r56	A	C	7: 12,711,027	M433R	probably benign	Het
Vmn2r76	C	A	7: 86,225,993	C592F	probably benign	Het
Zcchc8	C	T	5: 123,700,703	V591I	probably benign	Het
Znhit6	G	C	3: 145,576,654	G96A	probably benign	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- TGCCATCAGAAGGGAGGTTTG -3'
(R):5'- ACAGGTCTGAAGCACTGAGC -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2015-01-23