Incidental Mutation 'R2887:Zfp14'
| ID |
261084 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp14
|
| Ensembl Gene |
ENSMUSG00000053985 |
| Gene Name |
zinc finger protein 14 |
| Synonyms |
4732429I09Rik, Zfp-14, Krox-9 |
| MMRRC Submission |
040475-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R2887 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
29735784-29750805 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to T
at 29738190 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Lysine
at position 265
(T265K)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000146824
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000077787]
[ENSMUST00000207072]
[ENSMUST00000207873]
|
| AlphaFold |
P10755 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000077787
AA Change: T265K
PolyPhen 2
Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000076960
Gene: ENSMUSG00000053985
AA Change: T265K
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
8 |
69 |
2.39e-21 |
SMART |
|
ZnF_C2H2
|
140 |
162 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
168 |
190 |
5.9e-3 |
SMART |
|
ZnF_C2H2
|
196 |
218 |
2.75e-3 |
SMART |
|
ZnF_C2H2
|
224 |
246 |
6.42e-4 |
SMART |
|
ZnF_C2H2
|
252 |
274 |
1.03e-2 |
SMART |
|
ZnF_C2H2
|
280 |
302 |
5.5e-3 |
SMART |
|
ZnF_C2H2
|
308 |
330 |
2.02e-1 |
SMART |
|
ZnF_C2H2
|
336 |
358 |
8.6e-5 |
SMART |
|
ZnF_C2H2
|
364 |
386 |
4.17e-3 |
SMART |
|
ZnF_C2H2
|
392 |
414 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
420 |
442 |
9.44e-2 |
SMART |
|
ZnF_C2H2
|
448 |
470 |
1.03e-2 |
SMART |
|
ZnF_C2H2
|
476 |
498 |
5.9e-3 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000207072
AA Change: T265K
PolyPhen 2
Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000207873
AA Change: T265K
PolyPhen 2
Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.5%
- 10x: 97.1%
- 20x: 94.3%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 21 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1810009A15Rik |
T |
C |
19: 8,867,395 (GRCm39) |
S65P |
probably damaging |
Het |
| Adamts20 |
C |
T |
15: 94,228,459 (GRCm39) |
R996H |
probably benign |
Het |
| Aurka |
A |
G |
2: 172,209,040 (GRCm39) |
S54P |
probably benign |
Het |
| Clec7a |
C |
T |
6: 129,447,960 (GRCm39) |
G35E |
probably damaging |
Het |
| Clock |
G |
A |
5: 76,393,120 (GRCm39) |
R239C |
probably damaging |
Het |
| Eefsec |
T |
C |
6: 88,235,341 (GRCm39) |
T214A |
probably benign |
Het |
| Emg1 |
T |
C |
6: 124,682,026 (GRCm39) |
H166R |
probably damaging |
Het |
| Fibin |
T |
A |
2: 110,193,122 (GRCm39) |
I7F |
probably benign |
Het |
| Golm1 |
G |
T |
13: 59,788,044 (GRCm39) |
T285K |
probably benign |
Het |
| Ivl |
C |
A |
3: 92,478,699 (GRCm39) |
R455S |
unknown |
Het |
| Lama4 |
A |
C |
10: 38,968,250 (GRCm39) |
Q1464P |
possibly damaging |
Het |
| Lamp1 |
T |
A |
8: 13,223,891 (GRCm39) |
L341H |
probably damaging |
Het |
| Or13a28 |
A |
T |
7: 140,218,138 (GRCm39) |
I175F |
probably damaging |
Het |
| Ppp1r3a |
T |
G |
6: 14,718,248 (GRCm39) |
S889R |
possibly damaging |
Het |
| Ptprc |
A |
G |
1: 138,007,916 (GRCm39) |
V711A |
probably damaging |
Het |
| Rimklb |
T |
C |
6: 122,449,657 (GRCm39) |
T6A |
probably benign |
Het |
| Serpina3c |
T |
C |
12: 104,113,549 (GRCm39) |
H399R |
probably benign |
Het |
| Slc35a5 |
A |
G |
16: 44,971,923 (GRCm39) |
C114R |
probably damaging |
Het |
| Slc7a13 |
A |
G |
4: 19,819,052 (GRCm39) |
Y84C |
possibly damaging |
Het |
| Tcf7l1 |
G |
A |
6: 72,609,071 (GRCm39) |
S297L |
probably damaging |
Het |
| Vmn2r98 |
G |
T |
17: 19,301,439 (GRCm39) |
A814S |
possibly damaging |
Het |
|
| Other mutations in Zfp14 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00790:Zfp14
|
APN |
7 |
29,738,312 (GRCm39) |
nonsense |
probably null |
|
|
IGL01018:Zfp14
|
APN |
7 |
29,737,526 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01060:Zfp14
|
APN |
7 |
29,737,510 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03223:Zfp14
|
APN |
7 |
29,737,858 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0052:Zfp14
|
UTSW |
7 |
29,737,753 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0052:Zfp14
|
UTSW |
7 |
29,737,753 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1860:Zfp14
|
UTSW |
7 |
29,738,116 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2852:Zfp14
|
UTSW |
7 |
29,738,596 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4585:Zfp14
|
UTSW |
7 |
29,738,341 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4586:Zfp14
|
UTSW |
7 |
29,738,341 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4625:Zfp14
|
UTSW |
7 |
29,738,020 (GRCm39) |
nonsense |
probably null |
|
|
R4988:Zfp14
|
UTSW |
7 |
29,737,482 (GRCm39) |
missense |
probably benign |
|
|
R5791:Zfp14
|
UTSW |
7 |
29,737,687 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6709:Zfp14
|
UTSW |
7 |
29,737,557 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7799:Zfp14
|
UTSW |
7 |
29,738,368 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R8375:Zfp14
|
UTSW |
7 |
29,738,579 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R8784:Zfp14
|
UTSW |
7 |
29,742,961 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8997:Zfp14
|
UTSW |
7 |
29,737,600 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9721:Zfp14
|
UTSW |
7 |
29,738,609 (GRCm39) |
missense |
probably benign |
0.00 |
|
X0017:Zfp14
|
UTSW |
7 |
29,738,082 (GRCm39) |
missense |
probably damaging |
0.98 |
|
Z1186:Zfp14
|
UTSW |
7 |
29,738,577 (GRCm39) |
missense |
probably damaging |
0.98 |
|
| Predicted Primers |
PCR Primer
(F):5'- GAGTAGCTACTGAAGGTCTTCC -3'
(R):5'- ACAGTGCTCCAGGAACTCAC -3'
Sequencing Primer
(F):5'- TTCTCTGATGGCGAACAAGC -3'
(R):5'- TGCTCCAGGAACTCACACAGC -3'
|
| Posted On |
2015-01-23 |
Incidental Mutation