Incidental Mutation 'R0335:Tdo2'
ID 26162
Institutional Source Beutler Lab
Gene Symbol Tdo2
Ensembl Gene ENSMUSG00000028011
Gene Name tryptophan 2,3-dioxygenase
Synonyms chky, TO, TDO
MMRRC Submission 038544-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.148) question?
Stock # R0335 (G1)
Quality Score 204
Status Validated
Chromosome 3
Chromosomal Location 81957090-81976202 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 81964000 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Methionine to Leucine at position 235 (M235L)
Ref Sequence ENSEMBL: ENSMUSP00000141237 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029645] [ENSMUST00000193879]
AlphaFold P48776
Predicted Effect probably benign
Transcript: ENSMUST00000029645
AA Change: M254L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000029645
Gene: ENSMUSG00000028011
AA Change: M254L

DomainStartEndE-ValueType
Pfam:Trp_dioxygenase 26 372 8e-177 PFAM
low complexity region 393 406 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137974
Predicted Effect noncoding transcript
Transcript: ENSMUST00000151089
Predicted Effect probably benign
Transcript: ENSMUST00000193879
AA Change: M235L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000141237
Gene: ENSMUSG00000028011
AA Change: M235L

DomainStartEndE-ValueType
Pfam:Trp_dioxygenase 7 353 1.4e-174 PFAM
low complexity region 374 387 N/A INTRINSIC
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 98.8%
  • 3x: 97.6%
  • 10x: 94.6%
  • 20x: 87.5%
Validation Efficiency 100% (89/89)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism. [provided by RefSeq, Feb 2012]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased plasma and brain levels of tryptophan, increased serotonin levels in the brain, decreased anxiety-related behavior, increased neuronal precursor proliferation and accelerated neurogenesis in the granule cell layer of the olfactory bulb. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 88 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3830406C13Rik A G 14: 12,301,266 E124G possibly damaging Het
4932438A13Rik T C 3: 36,969,152 V2210A probably damaging Het
Adamts12 G T 15: 11,311,058 D1134Y possibly damaging Het
Add3 A G 19: 53,236,828 T460A probably benign Het
Amer3 A C 1: 34,579,300 probably benign Het
Arhgap22 C T 14: 33,359,108 probably benign Het
Arhgap32 T G 9: 32,259,760 S1279A probably benign Het
Bcas1 G A 2: 170,418,681 T26M probably damaging Het
Begain A T 12: 109,038,934 F256I probably damaging Het
Cabin1 A T 10: 75,657,049 I1804N probably damaging Het
Cad G A 5: 31,073,985 probably benign Het
Carmil1 G A 13: 24,073,983 S762L probably damaging Het
Ccdc93 T A 1: 121,492,977 L529Q probably damaging Het
Cdh12 T A 15: 21,578,549 probably null Het
Clip2 T A 5: 134,535,215 probably benign Het
Cmip T C 8: 117,445,366 I480T probably damaging Het
Cnot1 A T 8: 95,772,000 I203K probably benign Het
Col18a1 G A 10: 77,059,363 P1155S probably damaging Het
Col1a2 T A 6: 4,531,956 probably benign Het
Crybg3 A T 16: 59,544,140 L2373Q probably damaging Het
D130043K22Rik A T 13: 24,887,877 I935F probably damaging Het
Dapl1 T A 2: 59,496,594 D61E possibly damaging Het
Def6 A G 17: 28,228,069 D558G possibly damaging Het
Dnah6 T C 6: 73,069,399 probably benign Het
Dvl2 G A 11: 70,001,035 probably benign Het
Ecd A C 14: 20,320,734 V639G probably benign Het
Epg5 C T 18: 77,986,472 T1350M probably benign Het
Erbb4 C A 1: 68,259,259 M657I probably benign Het
Evi5 T C 5: 107,812,411 R431G probably benign Het
Fbxo11 G A 17: 88,015,613 A115V possibly damaging Het
Fgfr2 T C 7: 130,196,249 T192A probably benign Het
Gas7 C T 11: 67,662,052 A146V possibly damaging Het
Gatad2b T A 3: 90,356,182 S529T probably benign Het
Gm10722 G T 9: 3,001,048 Q41H probably null Het
Gm10801 G C 2: 98,664,007 R143T possibly damaging Het
Gm7535 A G 17: 17,911,112 probably benign Het
Gstm1 T A 3: 108,012,696 N193I possibly damaging Het
Heatr5b G A 17: 78,827,946 P252L probably benign Het
Hmgb1 A G 5: 149,050,631 V36A probably benign Het
Hrh1 G T 6: 114,480,232 W158L probably damaging Het
Ighv6-4 T C 12: 114,406,674 M53V probably benign Het
Iqgap2 T A 13: 95,635,633 D1346V probably damaging Het
Kcng3 A T 17: 83,587,737 N433K possibly damaging Het
Kif1a T A 1: 93,052,566 probably benign Het
Lctl C A 9: 64,118,887 Q75K probably benign Het
Ldb3 T A 14: 34,578,651 I89F possibly damaging Het
Lrrc49 A T 9: 60,677,095 L156Q probably damaging Het
Mark2 G T 19: 7,281,828 T83K probably benign Het
Ms4a15 A T 19: 10,980,210 D170E probably damaging Het
Msantd2 A G 9: 37,522,760 S99G possibly damaging Het
Nemf G T 12: 69,353,803 T124N probably benign Het
Nlrp9c A T 7: 26,394,136 F35I possibly damaging Het
Nwd2 A G 5: 63,804,773 I567V probably benign Het
Olfr111 A C 17: 37,530,642 I222L probably benign Het
Olfr1340 A G 4: 118,727,170 I308V probably null Het
Olfr323 T C 11: 58,625,740 Y102C probably damaging Het
Olfr828 T A 9: 18,815,994 Q100L probably damaging Het
Optn C T 2: 5,024,115 G526R probably damaging Het
Pdk4 T C 6: 5,491,138 E209G probably benign Het
Plch1 T C 3: 63,710,978 Q712R probably damaging Het
Pnpla1 T A 17: 28,886,878 V569E possibly damaging Het
Prkar2a A T 9: 108,719,258 D134V probably damaging Het
Ptov1 T A 7: 44,864,622 Q40L possibly damaging Het
Ptprq T C 10: 107,708,728 I314V probably benign Het
Rabl2 T C 15: 89,583,966 K66E probably damaging Het
Rnf38 A G 4: 44,152,507 V19A possibly damaging Het
Scn2a T A 2: 65,682,091 W191R probably damaging Het
Sec22b T A 3: 97,921,256 F212I possibly damaging Het
Sec24c T A 14: 20,688,715 probably null Het
Sept2 T C 1: 93,495,599 S51P probably damaging Het
Serpinb1a T C 13: 32,848,656 N90S probably damaging Het
Slc1a2 C T 2: 102,743,863 T206I probably benign Het
Slc25a19 C A 11: 115,624,206 R42L probably damaging Het
St14 G A 9: 31,091,324 probably benign Het
Stxbp1 C T 2: 32,802,905 probably benign Het
Tas2r131 C T 6: 132,957,829 V6I probably benign Het
Tenm3 T G 8: 48,232,105 H2432P probably damaging Het
Tmprss15 C T 16: 79,024,742 probably benign Het
Tmx1 A G 12: 70,453,256 N30D probably benign Het
Tom1 A G 8: 75,064,392 probably null Het
Top2a T C 11: 99,022,955 N20S probably benign Het
Ttc23l T A 15: 10,539,963 T145S probably benign Het
Unc13b T A 4: 43,236,983 M3351K possibly damaging Het
Vmn1r47 T C 6: 90,022,659 S258P probably damaging Het
Vmn2r8 T G 5: 108,797,451 probably null Het
Vps11 T C 9: 44,353,838 Q641R probably null Het
Wapl T A 14: 34,692,324 I381N probably damaging Het
Zmym6 G A 4: 127,122,808 G794E probably damaging Het
Other mutations in Tdo2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02127:Tdo2 APN 3 81958925 missense probably damaging 0.99
IGL02129:Tdo2 APN 3 81958925 missense probably damaging 0.99
IGL02271:Tdo2 APN 3 81963917 splice site probably benign
IGL02686:Tdo2 APN 3 81968155 missense probably benign 0.00
IGL02802:Tdo2 APN 3 81975697 intron probably benign
IGL03171:Tdo2 APN 3 81967029 missense probably benign
IGL03285:Tdo2 APN 3 81958789 splice site probably null
R0052:Tdo2 UTSW 3 81967025 missense probably benign 0.37
R0052:Tdo2 UTSW 3 81967025 missense probably benign 0.37
R0720:Tdo2 UTSW 3 81962758 missense probably damaging 1.00
R1174:Tdo2 UTSW 3 81974376 missense probably damaging 1.00
R1175:Tdo2 UTSW 3 81974376 missense probably damaging 1.00
R1222:Tdo2 UTSW 3 81961468 splice site probably null
R1418:Tdo2 UTSW 3 81961468 splice site probably null
R1868:Tdo2 UTSW 3 81960546 missense probably benign 0.04
R1918:Tdo2 UTSW 3 81958940 missense probably damaging 1.00
R2031:Tdo2 UTSW 3 81969505 missense probably damaging 1.00
R2513:Tdo2 UTSW 3 81969505 missense possibly damaging 0.91
R3615:Tdo2 UTSW 3 81975428 missense possibly damaging 0.68
R3616:Tdo2 UTSW 3 81975428 missense possibly damaging 0.68
R3872:Tdo2 UTSW 3 81968086 missense probably benign 0.08
R5260:Tdo2 UTSW 3 81975323 critical splice donor site probably null
R5547:Tdo2 UTSW 3 81958940 missense probably damaging 1.00
R6029:Tdo2 UTSW 3 81961440 missense probably damaging 1.00
R6089:Tdo2 UTSW 3 81962728 missense probably damaging 1.00
R6163:Tdo2 UTSW 3 81975403 missense possibly damaging 0.49
R6379:Tdo2 UTSW 3 81958795 unclassified probably benign
R7060:Tdo2 UTSW 3 81969559 missense probably damaging 1.00
R7544:Tdo2 UTSW 3 81971635 critical splice donor site probably null
R7585:Tdo2 UTSW 3 81962758 missense probably damaging 1.00
R7724:Tdo2 UTSW 3 81968083 critical splice donor site probably null
R8942:Tdo2 UTSW 3 81969544 missense probably benign 0.22
R9276:Tdo2 UTSW 3 81969578 missense probably benign
R9612:Tdo2 UTSW 3 81971694 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCAAACCGTTTAACCTGACTTCCCC -3'
(R):5'- GCTGCTTTGCCACCATCCAGATTAC -3'

Sequencing Primer
(F):5'- CCCAAGTTAGTCTTGATAGACAAGC -3'
(R):5'- TTACCAATTCCAAGTGGAGCTGG -3'
Posted On 2013-04-16