Mutagenetix > Incidental Mutation

Incidental Mutation 'R1034:Zfp758'

262398

Institutional Source

Beutler Lab

Gene Symbol

Zfp758

Ensembl Gene

ENSMUSG00000044501

Gene Name

zinc finger protein 758

Synonyms

MMRRC Submission

039133-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R1034 (G1)

Quality Score

107

Status

Not validated

Chromosome

Chromosomal Location

22361453-22377281 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 22375759 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 409 (E409*)

Ref Sequence

ENSEMBL: ENSMUSP00000121288 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072477] [ENSMUST00000088765] [ENSMUST00000121315] [ENSMUST00000149699]

AlphaFold

E9QAA1

Predicted Effect

probably null
Transcript: ENSMUST00000072477
AA Change: E377*

SMART Domains

Protein: ENSMUSP00000072298
Gene: ENSMUSG00000044501
AA Change: E377*

Domain	Start	End	E-Value	Type
KRAB	13	73	1.55e-20	SMART
ZnF_C2H2	177	199	6.92e0	SMART
ZnF_C2H2	205	227	6.32e-3	SMART
ZnF_C2H2	233	255	1.2e-3	SMART
ZnF_C2H2	261	283	9.08e-4	SMART
ZnF_C2H2	289	311	6.08e-5	SMART
ZnF_C2H2	317	339	1.03e-2	SMART
ZnF_C2H2	345	367	2.24e-3	SMART
ZnF_C2H2	373	395	3.21e-4	SMART
ZnF_C2H2	401	423	6.32e-3	SMART
ZnF_C2H2	429	451	2.24e-3	SMART
ZnF_C2H2	457	479	5.14e-3	SMART
ZnF_C2H2	485	507	1.04e-3	SMART
ZnF_C2H2	513	535	4.4e-2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000088765
AA Change: E377*

SMART Domains

Protein: ENSMUSP00000086143
Gene: ENSMUSG00000044501
AA Change: E377*

Domain	Start	End	E-Value	Type
KRAB	13	73	1.55e-20	SMART
ZnF_C2H2	177	199	6.92e0	SMART
ZnF_C2H2	205	227	6.32e-3	SMART
ZnF_C2H2	233	255	1.2e-3	SMART
ZnF_C2H2	261	283	9.08e-4	SMART
ZnF_C2H2	289	311	6.08e-5	SMART
ZnF_C2H2	317	339	1.03e-2	SMART
ZnF_C2H2	345	367	2.24e-3	SMART
ZnF_C2H2	373	395	3.21e-4	SMART
ZnF_C2H2	401	423	6.32e-3	SMART
ZnF_C2H2	429	451	2.24e-3	SMART
ZnF_C2H2	457	479	5.14e-3	SMART
ZnF_C2H2	485	507	1.04e-3	SMART
ZnF_C2H2	513	535	4.4e-2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000121315
AA Change: E377*

SMART Domains

Protein: ENSMUSP00000113532
Gene: ENSMUSG00000044501
AA Change: E377*

Domain	Start	End	E-Value	Type
KRAB	13	73	1.55e-20	SMART
ZnF_C2H2	177	199	6.92e0	SMART
ZnF_C2H2	205	227	6.32e-3	SMART
ZnF_C2H2	233	255	1.2e-3	SMART
ZnF_C2H2	261	283	9.08e-4	SMART
ZnF_C2H2	289	311	6.08e-5	SMART
ZnF_C2H2	317	339	1.03e-2	SMART
ZnF_C2H2	345	367	2.24e-3	SMART
ZnF_C2H2	373	395	3.21e-4	SMART
ZnF_C2H2	401	423	6.32e-3	SMART
ZnF_C2H2	429	451	2.24e-3	SMART
ZnF_C2H2	457	479	5.14e-3	SMART
ZnF_C2H2	485	507	1.04e-3	SMART
ZnF_C2H2	513	535	4.4e-2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000149699
AA Change: E409*

SMART Domains

Protein: ENSMUSP00000121288
Gene: ENSMUSG00000044501
AA Change: E409*

Domain	Start	End	E-Value	Type
KRAB	45	105	1.55e-20	SMART
ZnF_C2H2	209	231	6.92e0	SMART
ZnF_C2H2	237	259	6.32e-3	SMART
ZnF_C2H2	265	287	1.2e-3	SMART
ZnF_C2H2	293	315	9.08e-4	SMART
ZnF_C2H2	321	343	6.08e-5	SMART
ZnF_C2H2	349	371	1.03e-2	SMART
ZnF_C2H2	377	399	2.24e-3	SMART
ZnF_C2H2	405	427	3.21e-4	SMART
ZnF_C2H2	433	455	6.32e-3	SMART
ZnF_C2H2	461	483	2.24e-3	SMART
ZnF_C2H2	489	511	5.14e-3	SMART
ZnF_C2H2	517	539	1.04e-3	SMART
ZnF_C2H2	545	567	4.4e-2	SMART

Coding Region Coverage

1x: 98.7%
3x: 97.3%
10x: 92.9%
20x: 82.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aasdh	G	A	5: 76,876,283	T174M	probably damaging	Het
Abca14	T	C	7: 120,216,147	F206S	probably damaging	Het
Arid2	T	C	15: 96,369,505	V622A	probably benign	Het
Asic1	T	A	15: 99,698,058	L437Q	probably damaging	Het
Atp1b1	C	T	1: 164,453,488		probably null	Het
B3gnt5	A	G	16: 19,769,484	Y151C	probably damaging	Het
Cbx4	A	G	11: 119,081,707	S281P	probably damaging	Het
Dnah5	G	A	15: 28,302,471	V1625I	probably damaging	Het
Eftud2	A	C	11: 102,849,184	D461E	probably benign	Het
Epgn	A	G	5: 91,032,221	Y74C	probably damaging	Het
Fcho1	C	T	8: 71,712,560	A418T	probably benign	Het
Fgf14	C	T	14: 124,132,534	V113I	probably damaging	Het
Ggcx	G	A	6: 72,414,831	R68H	probably damaging	Het
Gm1110	A	G	9: 26,921,350	S16P	probably damaging	Het
Gm6729	T	C	10: 86,540,026		noncoding transcript	Het
Gpr108	A	G	17: 57,235,995	F522S	probably damaging	Het
Gpr156	T	C	16: 38,004,726	V435A	probably benign	Het
Khdrbs2	T	C	1: 32,467,791	L172P	probably damaging	Het
Kmo	C	T	1: 175,651,618	P240L	possibly damaging	Het
Ltn1	A	T	16: 87,397,137		probably null	Het
Nbeal1	T	A	1: 60,290,006	Y2194*	probably null	Het
Olfr767	T	A	10: 129,079,961	M1L	probably benign	Het
Olfr867	A	T	9: 20,055,365	S33T	probably benign	Het
Rab6b	T	C	9: 103,167,124	S172P	probably benign	Het
Ror1	T	A	4: 100,333,620	L58*	probably null	Het
Sec23b	A	G	2: 144,590,338	D756G	possibly damaging	Het
Slc24a2	T	A	4: 87,032,275	K428N	probably damaging	Het
Spen	C	A	4: 141,475,752	V1855L	probably benign	Het
Srgap1	G	A	10: 121,785,445	P1071S	possibly damaging	Het
Tns1	A	G	1: 73,941,969	C1079R	probably damaging	Het
Traf3ip1	G	T	1: 91,518,319		probably null	Het
Trmt2a	T	C	16: 18,249,709	F82S	probably damaging	Het
Xrn1	A	G	9: 96,039,737	D1401G	probably damaging	Het
Zfp352	T	A	4: 90,224,156	S178T	possibly damaging	Het

Other mutations in Zfp758

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01550:Zfp758	APN	17	22375040	missense	probably damaging	1.00
R1187:Zfp758	UTSW	17	22375190	missense	probably benign	0.23
R1733:Zfp758	UTSW	17	22375849	missense	probably damaging	1.00
R1847:Zfp758	UTSW	17	22375223	missense	probably benign	0.19
R1927:Zfp758	UTSW	17	22375842	missense	probably damaging	0.99
R1934:Zfp758	UTSW	17	22373652	missense	probably damaging	0.99
R2113:Zfp758	UTSW	17	22361645	missense	probably benign	0.10
R2132:Zfp758	UTSW	17	22375970	missense	probably damaging	1.00
R5134:Zfp758	UTSW	17	22375405	missense	probably damaging	1.00
R5274:Zfp758	UTSW	17	22375855	missense	probably benign	0.31
R5303:Zfp758	UTSW	17	22374861	missense	probably benign	0.05
R5394:Zfp758	UTSW	17	22372068	missense	probably damaging	0.99
R5813:Zfp758	UTSW	17	22375815	missense	probably damaging	1.00
R6017:Zfp758	UTSW	17	22373731	missense	probably damaging	1.00
R6411:Zfp758	UTSW	17	22375094	missense	possibly damaging	0.85
R6501:Zfp758	UTSW	17	22371997	intron	probably benign
R6805:Zfp758	UTSW	17	22361669	missense	probably benign	0.00
R7076:Zfp758	UTSW	17	22375156	missense	probably benign	0.11
R7147:Zfp758	UTSW	17	22376000	missense	possibly damaging	0.75
R7170:Zfp758	UTSW	17	22375135	nonsense	probably null
R7572:Zfp758	UTSW	17	22374891	missense	possibly damaging	0.61
R7593:Zfp758	UTSW	17	22374958	missense	probably damaging	0.98
R7699:Zfp758	UTSW	17	22375665	nonsense	probably null
R7858:Zfp758	UTSW	17	22375378	missense	probably benign	0.02
R9377:Zfp758	UTSW	17	22374944	missense	probably benign	0.35
R9717:Zfp758	UTSW	17	22374848	missense	possibly damaging	0.48

Predicted Primers

Posted On

2015-02-04