Incidental Mutation 'R0294:Spata31e5'
| ID |
26257 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Spata31e5
|
| Ensembl Gene |
ENSMUSG00000048411 |
| Gene Name |
spermatogenesis associated 31 subfamily E member 5 |
| Synonyms |
Gm597, LOC210962 |
| MMRRC Submission |
038511-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.057)
|
| Stock # |
R0294 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
1 |
| Chromosomal Location |
28776117-28780252 bp(-) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 28778663 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Glutamine to Arginine
at position 96
(Q96R)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000058140
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000059937]
|
| AlphaFold |
E9Q8J5 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000059937
AA Change: Q96R
PolyPhen 2
Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
|
| SMART Domains |
Protein: ENSMUSP00000058140
Gene: ENSMUSG00000048411
AA Change: Q96R
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
13 |
35 |
N/A |
INTRINSIC |
|
low complexity region
|
112 |
129 |
N/A |
INTRINSIC |
|
Pfam:FAM75
|
137 |
472 |
8.1e-14 |
PFAM |
|
low complexity region
|
664 |
675 |
N/A |
INTRINSIC |
|
internal_repeat_1
|
718 |
807 |
1.4e-5 |
PROSPERO |
|
internal_repeat_1
|
807 |
894 |
1.4e-5 |
PROSPERO |
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 97.0%
- 20x: 95.4%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 63 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930527J03Rik |
ACCC |
ACC |
1: 178,276,503 (GRCm38) |
|
noncoding transcript |
Het |
| Aadat |
A |
G |
8: 60,534,608 (GRCm38) |
E319G |
possibly damaging |
Het |
| Abca13 |
A |
G |
11: 9,269,122 (GRCm38) |
|
probably null |
Het |
| Actl7b |
A |
T |
4: 56,740,848 (GRCm38) |
L170Q |
possibly damaging |
Het |
| Adam29 |
C |
A |
8: 55,873,276 (GRCm38) |
V48L |
probably benign |
Het |
| Aknad1 |
A |
G |
3: 108,775,192 (GRCm38) |
Y528C |
probably damaging |
Het |
| Alas1 |
T |
A |
9: 106,241,256 (GRCm38) |
K222N |
probably damaging |
Het |
| Aplf |
A |
G |
6: 87,646,245 (GRCm38) |
V284A |
probably benign |
Het |
| Atp11a |
G |
A |
8: 12,827,524 (GRCm38) |
V317M |
probably benign |
Het |
| Bub3 |
A |
G |
7: 131,568,224 (GRCm38) |
E206G |
possibly damaging |
Het |
| Cblb |
T |
G |
16: 52,135,824 (GRCm38) |
F263L |
probably damaging |
Het |
| Ces2h |
T |
A |
8: 105,016,604 (GRCm38) |
M157K |
probably benign |
Het |
| Cfh |
A |
G |
1: 140,183,261 (GRCm38) |
F6L |
probably benign |
Het |
| Chst1 |
G |
T |
2: 92,613,642 (GRCm38) |
R153L |
probably damaging |
Het |
| Cimap2 |
T |
C |
4: 106,613,164 (GRCm38) |
D232G |
probably damaging |
Het |
| Cntnap5a |
T |
A |
1: 115,915,316 (GRCm38) |
N121K |
probably benign |
Het |
| Crybg1 |
A |
T |
10: 43,986,376 (GRCm38) |
S1467R |
probably damaging |
Het |
| Cyp2d22 |
A |
G |
15: 82,374,445 (GRCm38) |
F72L |
possibly damaging |
Het |
| Dmrt2 |
C |
T |
19: 25,678,071 (GRCm38) |
P345S |
probably damaging |
Het |
| Dock8 |
T |
C |
19: 25,188,350 (GRCm38) |
I1866T |
probably damaging |
Het |
| Egfem1 |
A |
G |
3: 29,690,121 (GRCm38) |
N503S |
probably damaging |
Het |
| Ehbp1 |
T |
C |
11: 22,095,427 (GRCm38) |
D774G |
probably benign |
Het |
| Foxp2 |
C |
A |
6: 15,376,774 (GRCm38) |
|
probably benign |
Het |
| Gins3 |
T |
C |
8: 95,637,919 (GRCm38) |
V99A |
possibly damaging |
Het |
| Grm1 |
A |
T |
10: 11,080,399 (GRCm38) |
I47N |
probably damaging |
Het |
| H2aj |
C |
G |
6: 136,808,604 (GRCm38) |
R89G |
probably damaging |
Het |
| Hsdl2 |
T |
A |
4: 59,601,408 (GRCm38) |
S127T |
probably benign |
Het |
| Il5ra |
A |
T |
6: 106,712,401 (GRCm38) |
M410K |
probably benign |
Het |
| Ints7 |
A |
G |
1: 191,611,891 (GRCm38) |
S548G |
possibly damaging |
Het |
| Kcnt1 |
A |
G |
2: 25,888,110 (GRCm38) |
E80G |
probably damaging |
Het |
| Lgr6 |
A |
G |
1: 134,987,891 (GRCm38) |
V373A |
probably damaging |
Het |
| Lgr6 |
T |
A |
1: 135,105,061 (GRCm38) |
Q27L |
unknown |
Het |
| Map3k14 |
T |
C |
11: 103,227,137 (GRCm38) |
I610V |
possibly damaging |
Het |
| Marf1 |
C |
T |
16: 14,142,534 (GRCm38) |
A549T |
probably damaging |
Het |
| Metap1d |
T |
A |
2: 71,522,545 (GRCm38) |
H239Q |
probably benign |
Het |
| Mgst2 |
A |
G |
3: 51,681,830 (GRCm38) |
Y88C |
probably damaging |
Het |
| Mroh4 |
T |
C |
15: 74,606,149 (GRCm38) |
N903D |
probably benign |
Het |
| Nbeal2 |
T |
G |
9: 110,632,859 (GRCm38) |
D1476A |
probably damaging |
Het |
| Nlgn1 |
C |
A |
3: 26,133,476 (GRCm38) |
A87S |
probably benign |
Het |
| Nln |
C |
T |
13: 104,052,579 (GRCm38) |
G295S |
probably damaging |
Het |
| Nnt |
T |
A |
13: 119,336,267 (GRCm38) |
Y719F |
probably benign |
Het |
| Nnt |
T |
G |
13: 119,338,417 (GRCm38) |
I659L |
possibly damaging |
Het |
| Or2z8 |
C |
T |
8: 72,058,400 (GRCm38) |
T240M |
probably damaging |
Het |
| Or51b17 |
A |
T |
7: 103,892,930 (GRCm38) |
H268Q |
probably benign |
Het |
| Or52s6 |
G |
A |
7: 103,443,084 (GRCm38) |
T13I |
possibly damaging |
Het |
| Or5p78 |
A |
T |
7: 108,613,150 (GRCm38) |
Y281F |
probably damaging |
Het |
| Or9g4 |
A |
G |
2: 85,674,716 (GRCm38) |
V145A |
probably damaging |
Het |
| Otogl |
C |
T |
10: 107,777,228 (GRCm38) |
C2041Y |
probably damaging |
Het |
| Patj |
G |
T |
4: 98,497,048 (GRCm38) |
D300Y |
probably damaging |
Het |
| Pkhd1l1 |
A |
T |
15: 44,560,435 (GRCm38) |
E3124D |
probably benign |
Het |
| Plbd2 |
A |
G |
5: 120,487,449 (GRCm38) |
|
probably null |
Het |
| Pphln1 |
T |
C |
15: 93,420,290 (GRCm38) |
Y57H |
probably damaging |
Het |
| Ppp1r16b |
C |
T |
2: 158,746,603 (GRCm38) |
T78M |
probably damaging |
Het |
| Prss40 |
T |
G |
1: 34,556,081 (GRCm38) |
D224A |
possibly damaging |
Het |
| Senp6 |
A |
G |
9: 80,113,725 (GRCm38) |
|
probably null |
Het |
| Shank3 |
A |
G |
15: 89,532,098 (GRCm38) |
E666G |
probably damaging |
Het |
| Slc13a1 |
T |
A |
6: 24,090,780 (GRCm38) |
I547F |
possibly damaging |
Het |
| Slc17a3 |
C |
T |
13: 23,855,858 (GRCm38) |
S293F |
probably damaging |
Het |
| Slc22a18 |
G |
A |
7: 143,492,841 (GRCm38) |
|
probably null |
Het |
| Slc5a4b |
A |
G |
10: 76,081,327 (GRCm38) |
C292R |
probably damaging |
Het |
| Sphkap |
T |
A |
1: 83,278,245 (GRCm38) |
E594D |
possibly damaging |
Het |
| Srpra |
T |
A |
9: 35,215,515 (GRCm38) |
M61K |
probably damaging |
Het |
| Trmt10c |
A |
T |
16: 56,034,877 (GRCm38) |
Y132N |
possibly damaging |
Het |
|
| Other mutations in Spata31e5 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00740:Spata31e5
|
APN |
1 |
28,778,651 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
IGL00885:Spata31e5
|
APN |
1 |
28,776,845 (GRCm38) |
missense |
unknown |
|
|
IGL01296:Spata31e5
|
APN |
1 |
28,777,056 (GRCm38) |
missense |
probably benign |
0.23 |
|
IGL01476:Spata31e5
|
APN |
1 |
28,777,453 (GRCm38) |
missense |
probably benign |
0.04 |
|
IGL02125:Spata31e5
|
APN |
1 |
28,776,338 (GRCm38) |
missense |
possibly damaging |
0.91 |
|
IGL02410:Spata31e5
|
APN |
1 |
28,778,631 (GRCm38) |
missense |
probably benign |
0.25 |
|
IGL02982:Spata31e5
|
APN |
1 |
28,778,054 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL03031:Spata31e5
|
APN |
1 |
28,778,583 (GRCm38) |
missense |
probably benign |
0.03 |
|
IGL03267:Spata31e5
|
APN |
1 |
28,777,121 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0433:Spata31e5
|
UTSW |
1 |
28,777,342 (GRCm38) |
nonsense |
probably null |
|
|
R0485:Spata31e5
|
UTSW |
1 |
28,778,142 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0645:Spata31e5
|
UTSW |
1 |
28,776,930 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0744:Spata31e5
|
UTSW |
1 |
28,777,821 (GRCm38) |
missense |
possibly damaging |
0.46 |
|
R0836:Spata31e5
|
UTSW |
1 |
28,777,821 (GRCm38) |
missense |
possibly damaging |
0.46 |
|
R1036:Spata31e5
|
UTSW |
1 |
28,777,802 (GRCm38) |
missense |
probably benign |
0.01 |
|
R1302:Spata31e5
|
UTSW |
1 |
28,776,340 (GRCm38) |
missense |
probably benign |
0.00 |
|
R1394:Spata31e5
|
UTSW |
1 |
28,776,809 (GRCm38) |
missense |
possibly damaging |
0.61 |
|
R1395:Spata31e5
|
UTSW |
1 |
28,776,809 (GRCm38) |
missense |
possibly damaging |
0.61 |
|
R1514:Spata31e5
|
UTSW |
1 |
28,778,748 (GRCm38) |
missense |
possibly damaging |
0.83 |
|
R1535:Spata31e5
|
UTSW |
1 |
28,777,424 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2004:Spata31e5
|
UTSW |
1 |
28,777,179 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2021:Spata31e5
|
UTSW |
1 |
28,778,153 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R2022:Spata31e5
|
UTSW |
1 |
28,778,153 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R3115:Spata31e5
|
UTSW |
1 |
28,776,329 (GRCm38) |
missense |
possibly damaging |
0.92 |
|
R3615:Spata31e5
|
UTSW |
1 |
28,776,575 (GRCm38) |
missense |
probably benign |
0.26 |
|
R3616:Spata31e5
|
UTSW |
1 |
28,776,575 (GRCm38) |
missense |
probably benign |
0.26 |
|
R3862:Spata31e5
|
UTSW |
1 |
28,777,641 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4067:Spata31e5
|
UTSW |
1 |
28,777,631 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4119:Spata31e5
|
UTSW |
1 |
28,777,973 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R4415:Spata31e5
|
UTSW |
1 |
28,777,133 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5010:Spata31e5
|
UTSW |
1 |
28,777,862 (GRCm38) |
missense |
possibly damaging |
0.52 |
|
R5109:Spata31e5
|
UTSW |
1 |
28,777,555 (GRCm38) |
missense |
possibly damaging |
0.46 |
|
R5122:Spata31e5
|
UTSW |
1 |
28,780,060 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5533:Spata31e5
|
UTSW |
1 |
28,778,082 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6085:Spata31e5
|
UTSW |
1 |
28,778,227 (GRCm38) |
missense |
possibly damaging |
0.55 |
|
R6116:Spata31e5
|
UTSW |
1 |
28,778,699 (GRCm38) |
missense |
probably benign |
0.01 |
|
R6750:Spata31e5
|
UTSW |
1 |
28,777,414 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R6757:Spata31e5
|
UTSW |
1 |
28,780,110 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R6774:Spata31e5
|
UTSW |
1 |
28,776,893 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7156:Spata31e5
|
UTSW |
1 |
28,776,767 (GRCm38) |
missense |
possibly damaging |
0.53 |
|
R7365:Spata31e5
|
UTSW |
1 |
28,780,152 (GRCm38) |
missense |
probably benign |
0.04 |
|
R7739:Spata31e5
|
UTSW |
1 |
28,777,608 (GRCm38) |
missense |
possibly damaging |
0.72 |
|
R7996:Spata31e5
|
UTSW |
1 |
28,778,406 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R8082:Spata31e5
|
UTSW |
1 |
28,777,498 (GRCm38) |
missense |
probably benign |
0.08 |
|
R8281:Spata31e5
|
UTSW |
1 |
28,778,144 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R8514:Spata31e5
|
UTSW |
1 |
28,778,505 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8944:Spata31e5
|
UTSW |
1 |
28,777,074 (GRCm38) |
missense |
probably benign |
0.00 |
|
R9042:Spata31e5
|
UTSW |
1 |
28,776,956 (GRCm38) |
missense |
possibly damaging |
0.72 |
|
R9101:Spata31e5
|
UTSW |
1 |
28,776,659 (GRCm38) |
missense |
probably benign |
0.04 |
|
R9106:Spata31e5
|
UTSW |
1 |
28,776,894 (GRCm38) |
missense |
probably benign |
0.00 |
|
R9173:Spata31e5
|
UTSW |
1 |
28,777,349 (GRCm38) |
missense |
probably benign |
0.22 |
|
R9596:Spata31e5
|
UTSW |
1 |
28,776,607 (GRCm38) |
missense |
probably benign |
0.07 |
|
R9632:Spata31e5
|
UTSW |
1 |
28,778,039 (GRCm38) |
missense |
probably benign |
0.20 |
|
R9656:Spata31e5
|
UTSW |
1 |
28,777,455 (GRCm38) |
missense |
probably benign |
0.02 |
|
R9659:Spata31e5
|
UTSW |
1 |
28,777,455 (GRCm38) |
missense |
probably benign |
0.02 |
|
R9661:Spata31e5
|
UTSW |
1 |
28,777,455 (GRCm38) |
missense |
probably benign |
0.02 |
|
R9663:Spata31e5
|
UTSW |
1 |
28,777,455 (GRCm38) |
missense |
probably benign |
0.02 |
|
R9710:Spata31e5
|
UTSW |
1 |
28,778,039 (GRCm38) |
missense |
probably benign |
0.20 |
|
| Predicted Primers |
PCR Primer
(F):5'- GTCTGGTCCTGACAAAACTGTGGAG -3'
(R):5'- TGTCATCCATGAGCACACAGAGC -3'
Sequencing Primer
(F):5'- TGGGCACTGTCAAGTTTACTAC -3'
(R):5'- AGGCAACTTGTCTTCCAGAG -3'
|
| Posted On |
2013-04-16 |
Incidental Mutation