Mutagenetix > Incidental Mutation

Incidental Mutation 'ANU23:Acot10'

262661

Institutional Source

Beutler Lab

Gene Symbol

Acot10

Ensembl Gene

ENSMUSG00000047565

Gene Name

acyl-CoA thioesterase 10

Synonyms

MT-ACT48, p48, Acate3

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.137) question?

Stock #

ANU23

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20665214-20666750 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 20665965 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 230 (F230S)

Ref Sequence

ENSEMBL: ENSMUSP00000051333 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052910]

AlphaFold

Q32MW3

Predicted Effect

probably benign
Transcript: ENSMUST00000052910
AA Change: F230S

PolyPhen 2 Score 0.109 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000051333
Gene: ENSMUSG00000047565
AA Change: F230S

Domain	Start	End	E-Value	Type
SCOP:d1lo7a_	108	222	1e-4	SMART
PDB:4IEN\|D	277	400	3e-6	PDB

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000226407

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227439

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000228652

Coding Region Coverage

1x: 98.7%
3x: 98.2%
10x: 96.6%
20x: 93.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd12b	G	A	12: 70,169,048	G88S	probably damaging	Het
Akap12	G	T	10: 4,353,273	A28S	probably benign	Het
Anks1	C	A	17: 27,986,253	T262K	probably damaging	Het
Arfgap3	A	G	15: 83,313,509	Y349H	possibly damaging	Het
Cacna1g	G	A	11: 94,466,228		probably benign	Het
Camsap2	T	A	1: 136,297,790	E199D	probably benign	Het
Ccdc13	A	T	9: 121,827,363	M128K	probably benign	Het
Ccdc38	T	C	10: 93,569,935		probably null	Het
Cep95	G	A	11: 106,813,815	V499I	probably benign	Het
Cpne6	G	C	14: 55,512,002	M6I	probably benign	Het
Cpne6	A	T	14: 55,515,249	I299F	probably damaging	Het
Cse1l	T	A	2: 166,927,508	Y278*	probably null	Het
Dip2c	A	G	13: 9,575,143	N558D	possibly damaging	Het
Edar	A	T	10: 58,628,638	C60S	probably damaging	Het
Fat2	T	C	11: 55,310,872	N459D	probably benign	Het
Fbxw8	C	T	5: 118,113,720	V243M	possibly damaging	Het
Fem1b	G	A	9: 62,797,528	A150V	possibly damaging	Het
Gm5422	A	T	10: 31,249,436		noncoding transcript	Het
Gpatch3	CAGGAGGAGGAGGAGGAG	CAGGAGGAGGAGGAG	4: 133,578,302		probably benign	Het
Grin2c	T	C	11: 115,256,194	T392A	probably benign	Het
Idh3a	T	A	9: 54,586,545		probably null	Het
Itpk1	T	C	12: 102,606,103	E117G	probably damaging	Het
Kif12	G	T	4: 63,165,884	P627Q	probably damaging	Het
Krtap15	T	A	16: 88,829,367	F88L	probably benign	Het
Mcm7	C	T	5: 138,170,391	V13I	probably benign	Het
Mlh1	T	C	9: 111,252,912	N248D	possibly damaging	Het
Olfr617	T	C	7: 103,584,693	Y224H	probably damaging	Het
Olfr727	A	G	14: 50,126,582	N2D	probably benign	Het
Olfr94	T	C	17: 37,196,942	N342S	probably benign	Het
Per2	T	C	1: 91,448,833	H106R	probably damaging	Het
Pfkl	T	A	10: 77,991,395	T486S	probably benign	Het
Prkdc	T	C	16: 15,667,731	V474A	possibly damaging	Het
Scamp4	C	A	10: 80,609,422	Q34K	probably damaging	Het
Serpinb3b	A	G	1: 107,154,665	Y290H	probably damaging	Het
Sft2d2	G	T	1: 165,183,995	A110E	probably benign	Het
Siglecf	T	A	7: 43,351,953	L115*	probably null	Het
Slc6a11	C	T	6: 114,134,665	T103M	probably damaging	Het
Slco1a1	T	A	6: 141,946,587	K18*	probably null	Het
Spata1	A	T	3: 146,487,399	Y112*	probably null	Het
Tbc1d32	G	A	10: 56,180,524	T440I	probably benign	Het
Vmn1r159	A	G	7: 22,843,163	V148A	probably damaging	Het
Wnt16	C	T	6: 22,297,935	R267C	probably damaging	Het
Xylt1	A	C	7: 117,548,890	S230R	probably benign	Het
Zfp334	T	C	2: 165,381,097	H342R	probably damaging	Het

Other mutations in Acot10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01306:Acot10	APN	15	20665965	missense	probably benign	0.11
IGL01610:Acot10	APN	15	20665695	missense	probably damaging	1.00
IGL02457:Acot10	APN	15	20666143	missense	possibly damaging	0.88
IGL02587:Acot10	APN	15	20665797	missense	possibly damaging	0.93
IGL02951:Acot10	APN	15	20665782	missense	probably benign	0.36
PIT4151001:Acot10	UTSW	15	20666598	missense	probably damaging	0.98
R0026:Acot10	UTSW	15	20666236	missense	probably benign	0.10
R0026:Acot10	UTSW	15	20666236	missense	probably benign	0.10
R0462:Acot10	UTSW	15	20666626	missense	possibly damaging	0.85
R1312:Acot10	UTSW	15	20666499	missense	probably benign	0.00
R1495:Acot10	UTSW	15	20665507	missense	probably damaging	0.99
R2128:Acot10	UTSW	15	20666626	missense	probably benign	0.00
R3779:Acot10	UTSW	15	20665542	missense	probably damaging	0.98
R4110:Acot10	UTSW	15	20666526	missense	probably damaging	1.00
R4111:Acot10	UTSW	15	20666526	missense	probably damaging	1.00
R4464:Acot10	UTSW	15	20665744	nonsense	probably null
R4668:Acot10	UTSW	15	20665942	missense	probably benign
R4933:Acot10	UTSW	15	20666330	missense	possibly damaging	0.88
R5255:Acot10	UTSW	15	20665932	missense	probably benign	0.01
R5885:Acot10	UTSW	15	20666104	missense	probably benign	0.01
R6190:Acot10	UTSW	15	20665785	missense	possibly damaging	0.80
R6301:Acot10	UTSW	15	20666262	missense	probably benign	0.05
R6805:Acot10	UTSW	15	20665366	missense	probably benign	0.42
R7334:Acot10	UTSW	15	20665543	missense	possibly damaging	0.86
R7601:Acot10	UTSW	15	20665629	missense	probably damaging	1.00
R8400:Acot10	UTSW	15	20666172	missense	possibly damaging	0.56
R9195:Acot10	UTSW	15	20665431	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCTACAAGCAGTAGCCCATGCAAG -3'
(R):5'- TGGGTACTGACCCTGAACTACGAG -3'

Sequencing Primer
(F):5'- CGTTCCTGAGGGTGACAAATATC -3'
(R):5'- CAGGACATTAAGTTCACTGGC -3'

Posted On

2015-02-04