Mutagenetix > Incidental Mutation

Incidental Mutation 'ANU74:Tns3'

262713

Institutional Source

Beutler Lab

Gene Symbol

Tns3

Ensembl Gene

ENSMUSG00000020422

Gene Name

tensin 3

Synonyms

TEM6, F830010I22Rik, Tens1

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.345) question?

Stock #

ANU74

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

8431652-8664535 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 8492149 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 738 (R738Q)

Ref Sequence

ENSEMBL: ENSMUSP00000020695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020695]

AlphaFold

Q5SSZ5

Predicted Effect

probably benign
Transcript: ENSMUST00000020695
AA Change: R738Q

PolyPhen 2 Score 0.383 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000020695
Gene: ENSMUSG00000020422
AA Change: R738Q

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	1	171	5e-28	SMART
PTEN_C2	173	300	1.15e-48	SMART
low complexity region	854	864	N/A	INTRINSIC
low complexity region	1102	1126	N/A	INTRINSIC
SH2	1165	1268	1.32e-18	SMART
PTB	1301	1438	3.14e-24	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.2%
20x: 95.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit one third postnatal lethality, reduced body weight, growth retardation, smaller digestive tracts with defects in villi and enterocyte differentiation, abnormal lung morphology, and thinner bones with decreased chondrocyte proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgra3	G	A	5: 49,961,038	S1056L	probably benign	Het
Ankrd26	A	T	6: 118,552,775	D236E	probably benign	Het
Capn15	C	T	17: 25,965,486	W7*	probably null	Het
Celsr2	G	A	3: 108,412,499	T999M	probably damaging	Het
Chrd	T	A	16: 20,741,319	M912K	possibly damaging	Het
Col9a1	A	G	1: 24,185,328	D197G	unknown	Het
Csf1r	A	G	18: 61,117,391	E431G	probably benign	Het
Eloc	A	G	1: 16,643,350	F115L	possibly damaging	Het
Fap	T	C	2: 62,547,769	D193G	probably damaging	Het
Fscn2	T	C	11: 120,362,336	Y210H	probably damaging	Het
Fut9	G	C	4: 25,620,802	T4R	probably benign	Het
Grb2	A	T	11: 115,645,907	D131E	probably benign	Het
Hecw2	T	C	1: 53,925,694	T658A	probably benign	Het
Helz2	T	C	2: 181,234,834	E1289G	probably benign	Het
Hyou1	A	G	9: 44,381,263	N92D	possibly damaging	Het
Irf8	A	C	8: 120,739,869	I18L	possibly damaging	Het
Kif12	GGGGC	GGGGCCTCCACCCGGCGGGC	4: 63,171,423		probably benign	Het
Kif12	GC	"GCCTCCACCCGGCGGTC,GCC"	4: 63,171,426		probably null	Het
Lamc2	A	C	1: 153,131,835	D864E	probably benign	Het
Map3k4	A	G	17: 12,232,976	V1475A	probably damaging	Het
Mapk8ip3	A	C	17: 24,900,577	M1030R	possibly damaging	Het
Mat1a	A	G	14: 41,111,142	D94G	probably benign	Het
Myh15	T	A	16: 49,172,932	D1643E	possibly damaging	Het
Myo15b	T	C	11: 115,878,413	F55L	probably damaging	Het
Nhlh2	A	T	3: 102,012,654	M1L	probably benign	Het
Nup214	T	C	2: 32,034,966	S1836P	probably damaging	Het
Olfr1034	T	A	2: 86,047,311	Y276*	probably null	Het
Olr1	G	A	6: 129,500,069	R78C	possibly damaging	Het
Pam16	C	A	16: 4,616,633	V98F	probably damaging	Het
Pelp1	C	T	11: 70,395,087	V652I	probably damaging	Het
Pole	A	G	5: 110,289,370	H67R	probably benign	Het
Rbms1	G	T	2: 60,797,716	A60E	probably damaging	Het
Recql4	A	T	15: 76,705,757	M789K	possibly damaging	Het
Rrn3	T	C	16: 13,811,533	F571S	possibly damaging	Het
Ryr3	T	C	2: 112,831,230		probably null	Het
Sec61b	C	A	4: 47,474,922	N26K	possibly damaging	Het
Serinc1	T	C	10: 57,519,842	E284G	probably benign	Het
Slc30a9	G	A	5: 67,349,852	D496N	probably damaging	Het
Slc44a4	G	A	17: 34,921,578	R249H	probably damaging	Het
Slc6a13	A	C	6: 121,334,876	D404A	probably benign	Het
Spata18	A	G	5: 73,671,113	E225G	probably damaging	Het
Sspo	G	A	6: 48,460,959	G1351S	probably damaging	Het
Tgm3	T	C	2: 130,048,390	V691A	probably damaging	Het
Tyk2	A	T	9: 21,116,158	I506N	probably damaging	Het
Ube2v1	G	A	2: 167,610,344	T113I	probably damaging	Het
Vmn1r177	A	G	7: 23,866,220	V77A	possibly damaging	Het
Vmn2r14	A	T	5: 109,219,044	S437T	probably benign	Het
Vmn2r78	G	C	7: 86,921,065	V264L	possibly damaging	Het
Zfp956	T	G	6: 47,963,573	Y289D	probably benign	Het

Other mutations in Tns3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Tns3	APN	11	8451066	missense	probably benign	0.42
IGL00822:Tns3	APN	11	8443976	missense	probably damaging	0.99
IGL01075:Tns3	APN	11	8478399	missense	probably benign	0.45
IGL01286:Tns3	APN	11	8492617	missense	probably benign	0.01
IGL01680:Tns3	APN	11	8548937	missense	probably damaging	1.00
IGL01687:Tns3	APN	11	8492798	missense	probably damaging	1.00
IGL01734:Tns3	APN	11	8519192	splice site	probably benign
IGL01844:Tns3	APN	11	8437177	missense	possibly damaging	0.58
IGL01984:Tns3	APN	11	8548992	nonsense	probably null
IGL02137:Tns3	APN	11	8492578	missense	possibly damaging	0.93
IGL02273:Tns3	APN	11	8434531	missense	probably damaging	1.00
IGL02623:Tns3	APN	11	8437141	missense	probably damaging	1.00
IGL02697:Tns3	APN	11	8492346	missense	probably benign	0.00
IGL02829:Tns3	APN	11	8519564	missense	probably damaging	1.00
R0020:Tns3	UTSW	11	8545227	critical splice donor site	probably null
R0064:Tns3	UTSW	11	8435856	nonsense	probably null
R0064:Tns3	UTSW	11	8435856	nonsense	probably null
R0370:Tns3	UTSW	11	8445730	missense	possibly damaging	0.80
R0388:Tns3	UTSW	11	8445703	missense	probably benign	0.07
R0410:Tns3	UTSW	11	8435852	missense	probably benign	0.02
R0496:Tns3	UTSW	11	8547262	splice site	probably benign
R0562:Tns3	UTSW	11	8493262	missense	possibly damaging	0.93
R0626:Tns3	UTSW	11	8493121	missense	probably benign	0.04
R0736:Tns3	UTSW	11	8519474	missense	possibly damaging	0.94
R0893:Tns3	UTSW	11	8493302	missense	probably damaging	1.00
R1367:Tns3	UTSW	11	8448704	missense	probably benign	0.01
R1386:Tns3	UTSW	11	8518261	missense	probably benign	0.02
R1975:Tns3	UTSW	11	8435738	missense	probably benign	0.04
R2205:Tns3	UTSW	11	8531719	missense	probably damaging	1.00
R2319:Tns3	UTSW	11	8541200	missense	probably damaging	1.00
R2830:Tns3	UTSW	11	8435870	missense	probably damaging	1.00
R3720:Tns3	UTSW	11	8492999	missense	probably damaging	1.00
R3765:Tns3	UTSW	11	8451133	missense	probably benign	0.00
R3817:Tns3	UTSW	11	8434619	missense	probably damaging	1.00
R4058:Tns3	UTSW	11	8492275	missense	probably damaging	1.00
R4599:Tns3	UTSW	11	8531747	missense	probably damaging	1.00
R4631:Tns3	UTSW	11	8451119	missense	probably benign	0.30
R4731:Tns3	UTSW	11	8450986	missense	probably benign	0.28
R4732:Tns3	UTSW	11	8450986	missense	probably benign	0.28
R4733:Tns3	UTSW	11	8450986	missense	probably benign	0.28
R5472:Tns3	UTSW	11	8451092	missense	probably benign
R5749:Tns3	UTSW	11	8451177	missense	probably benign	0.01
R5807:Tns3	UTSW	11	8493211	missense	probably damaging	1.00
R5844:Tns3	UTSW	11	8434580	missense	probably damaging	1.00
R5942:Tns3	UTSW	11	8435860	missense	probably damaging	1.00
R5982:Tns3	UTSW	11	8492245	missense	probably damaging	0.99
R6025:Tns3	UTSW	11	8492578	missense	possibly damaging	0.93
R6266:Tns3	UTSW	11	8492987	missense	probably damaging	1.00
R6322:Tns3	UTSW	11	8492147	missense	probably benign	0.01
R6536:Tns3	UTSW	11	8434531	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8549057	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8549058	missense	probably damaging	1.00
R6864:Tns3	UTSW	11	8493196	missense	probably damaging	1.00
R6897:Tns3	UTSW	11	8531743	missense	probably damaging	1.00
R7108:Tns3	UTSW	11	8437251	missense	probably benign	0.00
R7443:Tns3	UTSW	11	8451442	missense	probably benign	0.01
R7459:Tns3	UTSW	11	8492793	missense	probably benign	0.16
R7474:Tns3	UTSW	11	8530894	missense	probably damaging	1.00
R7576:Tns3	UTSW	11	8541192	missense	possibly damaging	0.78
R7979:Tns3	UTSW	11	8492701	missense	probably benign	0.01
R8055:Tns3	UTSW	11	8545343	missense	probably damaging	1.00
R8057:Tns3	UTSW	11	8492773	missense	probably benign
R8077:Tns3	UTSW	11	8445667	missense	probably damaging	1.00
R8518:Tns3	UTSW	11	8492971	missense	probably damaging	0.96
R8523:Tns3	UTSW	11	8448779	missense	probably damaging	1.00
R8790:Tns3	UTSW	11	8518273	missense	probably damaging	0.99
R9228:Tns3	UTSW	11	8450094	missense	probably damaging	1.00
R9374:Tns3	UTSW	11	8492606	missense	probably damaging	1.00
R9476:Tns3	UTSW	11	8445702	missense	probably damaging	0.99
R9510:Tns3	UTSW	11	8445702	missense	probably damaging	0.99
R9594:Tns3	UTSW	11	8451142	missense	possibly damaging	0.79
R9595:Tns3	UTSW	11	8451142	missense	possibly damaging	0.79
R9596:Tns3	UTSW	11	8451142	missense	possibly damaging	0.79
R9624:Tns3	UTSW	11	8451142	missense	possibly damaging	0.79
R9629:Tns3	UTSW	11	8451142	missense	possibly damaging	0.79
T0975:Tns3	UTSW	11	8451146	missense	probably benign	0.00
T0975:Tns3	UTSW	11	8479518	missense	probably benign
T0975:Tns3	UTSW	11	8549100	start gained	probably benign
X0005:Tns3	UTSW	11	8451224	missense	probably benign	0.00
X0005:Tns3	UTSW	11	8479518	missense	probably benign
Z1177:Tns3	UTSW	11	8451014	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGTGAGAGTAGAAAATGCCGCTCC -3'
(R):5'- CACAGCAGCTCTGTCCTGGTAAAG -3'

Sequencing Primer
(F):5'- TCCATCATCAGTTTCAGCAAATC -3'
(R):5'- TCACGAATGGAGTCCATCAG -3'

Posted On

2015-02-04