Mutagenetix > Incidental Mutation

Incidental Mutation 'R3103:Golgb1'

262926

Institutional Source

Beutler Lab

Gene Symbol

Golgb1

Ensembl Gene

ENSMUSG00000034243

Gene Name

golgi autoantigen, golgin subfamily b, macrogolgin 1

Synonyms

Giantin, C130074L01Rik, F730017E11Rik, Gm6840, 6330407A06Rik

MMRRC Submission

040577-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.911) question?

Stock #

R3103 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

36875140-36933085 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 36894849 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glycine at position 226 (R226G)

Ref Sequence

ENSEMBL: ENSMUSP00000110460 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039855] [ENSMUST00000114812]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000039855
AA Change: R267G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000045239
Gene: ENSMUSG00000034243
AA Change: R267G

Domain	Start	End	E-Value	Type
internal_repeat_2	24	61	7.47e-6	PROSPERO
low complexity region	87	107	N/A	INTRINSIC
coiled coil region	130	219	N/A	INTRINSIC
low complexity region	491	512	N/A	INTRINSIC
internal_repeat_3	519	558	7.47e-6	PROSPERO
coiled coil region	563	594	N/A	INTRINSIC
internal_repeat_4	627	661	3.38e-5	PROSPERO
coiled coil region	679	1121	N/A	INTRINSIC
coiled coil region	1153	1240	N/A	INTRINSIC
internal_repeat_4	1253	1288	3.38e-5	PROSPERO
low complexity region	1300	1314	N/A	INTRINSIC
internal_repeat_1	1321	1352	3.51e-6	PROSPERO
low complexity region	1357	1369	N/A	INTRINSIC
coiled coil region	1402	1755	N/A	INTRINSIC
internal_repeat_2	1760	1798	7.47e-6	PROSPERO
internal_repeat_3	1761	1804	7.47e-6	PROSPERO
coiled coil region	1818	2034	N/A	INTRINSIC
low complexity region	2291	2306	N/A	INTRINSIC
internal_repeat_1	2351	2382	3.51e-6	PROSPERO
low complexity region	2400	2418	N/A	INTRINSIC
low complexity region	2538	2549	N/A	INTRINSIC
coiled coil region	2775	2827	N/A	INTRINSIC
coiled coil region	2854	2943	N/A	INTRINSIC
low complexity region	2964	2976	N/A	INTRINSIC
coiled coil region	3007	3057	N/A	INTRINSIC
coiled coil region	3117	3163	N/A	INTRINSIC
transmembrane domain	3215	3237	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114812
AA Change: R226G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110460
Gene: ENSMUSG00000034243
AA Change: R226G

Domain	Start	End	E-Value	Type
internal_repeat_2	24	61	6.71e-6	PROSPERO
low complexity region	87	107	N/A	INTRINSIC
low complexity region	120	134	N/A	INTRINSIC
low complexity region	143	154	N/A	INTRINSIC
low complexity region	200	219	N/A	INTRINSIC
low complexity region	450	471	N/A	INTRINSIC
internal_repeat_3	478	517	6.71e-6	PROSPERO
coiled coil region	522	553	N/A	INTRINSIC
internal_repeat_4	586	620	3.05e-5	PROSPERO
coiled coil region	638	1080	N/A	INTRINSIC
coiled coil region	1112	1199	N/A	INTRINSIC
internal_repeat_4	1212	1247	3.05e-5	PROSPERO
low complexity region	1259	1273	N/A	INTRINSIC
internal_repeat_1	1280	1311	3.14e-6	PROSPERO
low complexity region	1316	1328	N/A	INTRINSIC
coiled coil region	1361	1714	N/A	INTRINSIC
internal_repeat_2	1719	1757	6.71e-6	PROSPERO
internal_repeat_3	1720	1763	6.71e-6	PROSPERO
coiled coil region	1777	1993	N/A	INTRINSIC
low complexity region	2250	2265	N/A	INTRINSIC
internal_repeat_1	2310	2341	3.14e-6	PROSPERO
low complexity region	2359	2377	N/A	INTRINSIC
low complexity region	2497	2508	N/A	INTRINSIC
coiled coil region	2734	2786	N/A	INTRINSIC
coiled coil region	2813	2902	N/A	INTRINSIC
low complexity region	2923	2935	N/A	INTRINSIC
coiled coil region	2966	3016	N/A	INTRINSIC
coiled coil region	3076	3122	N/A	INTRINSIC
transmembrane domain	3174	3196	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153572

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000231790

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous knockout affects glycosylation of glycoproteins in the extra-cellular matrix of the palatal shelves, resulting in their failure to elevate and fuse, leading to cleft palate. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted, other(2) Gene trapped(5)

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acp7	A	G	7: 28,610,984 (GRCm38)		probably null	Het
Adap2	G	T	11: 80,157,033 (GRCm38)	C105F	probably damaging	Het
Bace2	T	C	16: 97,422,001 (GRCm38)		probably null	Het
Bpifc	A	G	10: 85,993,422 (GRCm38)	S94P	probably damaging	Het
C2cd3	A	G	7: 100,395,252 (GRCm38)	D347G	possibly damaging	Het
Cad	T	C	5: 31,061,674 (GRCm38)	V613A	possibly damaging	Het
Ccdc47	T	C	11: 106,202,841 (GRCm38)	H6R	probably benign	Het
Celsr3	A	G	9: 108,837,139 (GRCm38)	T1956A	probably benign	Het
Cep128	T	A	12: 91,019,344 (GRCm38)	D1006V	probably damaging	Het
Cog3	T	C	14: 75,747,201 (GRCm38)		probably null	Het
Csmd1	C	T	8: 15,917,405 (GRCm38)	V3153M	probably damaging	Het
Ctnna2	T	C	6: 77,653,144 (GRCm38)	E122G	possibly damaging	Het
Cts8	T	A	13: 61,250,958 (GRCm38)	I245F	probably damaging	Het
Ddx27	T	A	2: 167,026,246 (GRCm38)	V333E	probably damaging	Het
Dmpk	A	G	7: 19,087,654 (GRCm38)	Y279C	probably damaging	Het
Dpagt1	A	G	9: 44,327,995 (GRCm38)	I111V	probably benign	Het
Dvl2	C	A	11: 70,008,869 (GRCm38)	P546T	possibly damaging	Het
Fat4	G	T	3: 38,891,940 (GRCm38)	A1661S	probably benign	Het
Gcm1	A	G	9: 78,064,452 (GRCm38)	N225S	probably damaging	Het
Gcnt2	A	T	13: 40,918,606 (GRCm38)	M242L	probably benign	Het
Gpr63	G	A	4: 25,007,353 (GRCm38)	V26I	probably benign	Het
Grik2	A	G	10: 49,240,772 (GRCm38)	L631P	probably damaging	Het
Hapln3	T	C	7: 79,121,736 (GRCm38)	D135G	probably benign	Het
Il31ra	C	A	13: 112,530,351 (GRCm38)	V398F	probably damaging	Het
Ipp	G	T	4: 116,524,249 (GRCm38)	R315L	possibly damaging	Het
Kcmf1	A	G	6: 72,861,847 (GRCm38)	L32P	probably damaging	Het
Klf17	T	A	4: 117,760,608 (GRCm38)	Q184L	possibly damaging	Het
Lrp2	C	T	2: 69,431,984 (GRCm38)	V4442I	probably benign	Het
Lrrfip2	A	T	9: 111,222,210 (GRCm38)	E293D	probably damaging	Het
Oit3	A	G	10: 59,438,891 (GRCm38)	I29T	probably damaging	Het
Olfr403	G	A	11: 74,196,075 (GRCm38)	D191N	probably benign	Het
Olfr50	T	C	2: 36,793,562 (GRCm38)	C109R	possibly damaging	Het
Olfr555	T	C	7: 102,659,481 (GRCm38)	V220A	probably benign	Het
Plb1	T	A	5: 32,328,029 (GRCm38)	M842K	possibly damaging	Het
Ppt1	T	C	4: 122,836,307 (GRCm38)	C18R	probably benign	Het
Pstpip2	T	C	18: 77,871,777 (GRCm38)	Y191H	probably damaging	Het
Ryr1	C	T	7: 29,074,948 (GRCm38)	V2361I	probably damaging	Het
Serpinb11	A	G	1: 107,377,608 (GRCm38)	N238S	probably benign	Het
Skor2	A	T	18: 76,859,278 (GRCm38)	K232*	probably null	Het
Slc13a5	A	T	11: 72,257,388 (GRCm38)	W231R	probably damaging	Het
Svs5	A	T	2: 164,333,393 (GRCm38)	E55V	probably benign	Het
Tfcp2	A	T	15: 100,525,600 (GRCm38)	W142R	probably damaging	Het
Trpc2	A	T	7: 102,095,234 (GRCm38)	I738F	possibly damaging	Het
Vmn2r61	T	A	7: 42,266,643 (GRCm38)	S227T	possibly damaging	Het
Zfhx4	A	G	3: 5,399,326 (GRCm38)	T1540A	probably damaging	Het
Zfp616	A	G	11: 74,071,735 (GRCm38)	T74A	probably benign	Het

Other mutations in Golgb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01394:Golgb1	APN	16	36,931,564 (GRCm38)	missense	probably damaging	1.00
IGL01717:Golgb1	APN	16	36,915,502 (GRCm38)	nonsense	probably null
IGL01965:Golgb1	APN	16	36,917,920 (GRCm38)	missense	probably damaging	1.00
IGL02128:Golgb1	APN	16	36,916,304 (GRCm38)	missense	probably damaging	1.00
IGL02268:Golgb1	APN	16	36,913,128 (GRCm38)	missense	probably benign	0.25
IGL02383:Golgb1	APN	16	36,886,200 (GRCm38)	missense	probably benign	0.01
IGL02444:Golgb1	APN	16	36,907,816 (GRCm38)	splice site	probably benign
IGL02635:Golgb1	APN	16	36,915,013 (GRCm38)	missense	probably benign	0.00
IGL02655:Golgb1	APN	16	36,918,080 (GRCm38)	missense	probably damaging	0.98
IGL02887:Golgb1	APN	16	36,925,849 (GRCm38)	missense	probably damaging	0.99
IGL02937:Golgb1	APN	16	36,916,210 (GRCm38)	missense	probably damaging	1.00
IGL02973:Golgb1	APN	16	36,912,080 (GRCm38)	missense	possibly damaging	0.92
IGL02982:Golgb1	APN	16	36,925,810 (GRCm38)	missense	probably damaging	0.98
IGL03065:Golgb1	APN	16	36,912,866 (GRCm38)	missense	probably benign	0.11
IGL03109:Golgb1	APN	16	36,915,611 (GRCm38)	missense	possibly damaging	0.93
IGL03323:Golgb1	APN	16	36,913,453 (GRCm38)	nonsense	probably null
I2288:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
I2289:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
R0071:Golgb1	UTSW	16	36,915,503 (GRCm38)	missense	probably benign	0.00
R0071:Golgb1	UTSW	16	36,915,503 (GRCm38)	missense	probably benign	0.00
R0080:Golgb1	UTSW	16	36,898,611 (GRCm38)	missense	probably damaging	1.00
R0102:Golgb1	UTSW	16	36,875,468 (GRCm38)	intron	probably benign
R0242:Golgb1	UTSW	16	36,875,630 (GRCm38)	nonsense	probably null
R0242:Golgb1	UTSW	16	36,875,630 (GRCm38)	nonsense	probably null
R0276:Golgb1	UTSW	16	36,913,876 (GRCm38)	missense	probably damaging	1.00
R0394:Golgb1	UTSW	16	36,875,579 (GRCm38)	intron	probably benign
R0469:Golgb1	UTSW	16	36,931,635 (GRCm38)	missense	probably benign	0.41
R0522:Golgb1	UTSW	16	36,915,205 (GRCm38)	frame shift	probably null
R0575:Golgb1	UTSW	16	36,918,809 (GRCm38)	missense	probably benign
R0600:Golgb1	UTSW	16	36,916,271 (GRCm38)	missense	probably damaging	1.00
R0608:Golgb1	UTSW	16	36,916,330 (GRCm38)	nonsense	probably null
R0711:Golgb1	UTSW	16	36,918,790 (GRCm38)	missense	probably damaging	1.00
R0785:Golgb1	UTSW	16	36,898,790 (GRCm38)	missense	possibly damaging	0.95
R0893:Golgb1	UTSW	16	36,912,277 (GRCm38)	missense	possibly damaging	0.64
R1163:Golgb1	UTSW	16	36,916,126 (GRCm38)	missense	possibly damaging	0.50
R1208:Golgb1	UTSW	16	36,915,205 (GRCm38)	frame shift	probably null
R1315:Golgb1	UTSW	16	36,914,900 (GRCm38)	missense	probably benign	0.40
R1429:Golgb1	UTSW	16	36,900,563 (GRCm38)	missense	possibly damaging	0.93
R1505:Golgb1	UTSW	16	36,919,643 (GRCm38)	missense	possibly damaging	0.79
R1537:Golgb1	UTSW	16	36,898,788 (GRCm38)	missense	possibly damaging	0.89
R1610:Golgb1	UTSW	16	36,926,101 (GRCm38)	missense	probably benign	0.25
R1659:Golgb1	UTSW	16	36,887,617 (GRCm38)	missense	probably benign	0.01
R1769:Golgb1	UTSW	16	36,916,001 (GRCm38)	missense	probably damaging	1.00
R2105:Golgb1	UTSW	16	36,914,664 (GRCm38)	missense	probably benign
R2212:Golgb1	UTSW	16	36,887,347 (GRCm38)	missense	probably damaging	1.00
R2261:Golgb1	UTSW	16	36,893,360 (GRCm38)	missense	probably damaging	1.00
R2352:Golgb1	UTSW	16	36,898,559 (GRCm38)	missense	probably damaging	0.99
R2357:Golgb1	UTSW	16	36,912,008 (GRCm38)	missense	probably damaging	1.00
R2400:Golgb1	UTSW	16	36,918,466 (GRCm38)	missense	possibly damaging	0.62
R2513:Golgb1	UTSW	16	36,915,151 (GRCm38)	missense	possibly damaging	0.73
R3413:Golgb1	UTSW	16	36,887,347 (GRCm38)	missense	probably damaging	1.00
R3748:Golgb1	UTSW	16	36,918,912 (GRCm38)	missense	probably benign	0.00
R3847:Golgb1	UTSW	16	36,898,733 (GRCm38)	missense	probably benign	0.00
R3850:Golgb1	UTSW	16	36,898,733 (GRCm38)	missense	probably benign	0.00
R3936:Golgb1	UTSW	16	36,914,056 (GRCm38)	nonsense	probably null
R3975:Golgb1	UTSW	16	36,918,571 (GRCm38)	missense	probably damaging	0.99
R4025:Golgb1	UTSW	16	36,915,344 (GRCm38)	missense	probably benign	0.00
R4369:Golgb1	UTSW	16	36,916,907 (GRCm38)	missense	probably damaging	1.00
R4518:Golgb1	UTSW	16	36,929,263 (GRCm38)	missense	probably damaging	0.98
R4600:Golgb1	UTSW	16	36,918,625 (GRCm38)	missense	probably damaging	1.00
R4610:Golgb1	UTSW	16	36,918,625 (GRCm38)	missense	probably damaging	1.00
R4660:Golgb1	UTSW	16	36,887,618 (GRCm38)	missense	probably damaging	0.99
R4811:Golgb1	UTSW	16	36,891,419 (GRCm38)	missense	probably damaging	1.00
R4815:Golgb1	UTSW	16	36,913,115 (GRCm38)	missense	possibly damaging	0.79
R4835:Golgb1	UTSW	16	36,891,407 (GRCm38)	missense	possibly damaging	0.86
R4904:Golgb1	UTSW	16	36,893,386 (GRCm38)	missense	probably damaging	1.00
R4916:Golgb1	UTSW	16	36,916,118 (GRCm38)	missense	probably benign	0.05
R5121:Golgb1	UTSW	16	36,919,258 (GRCm38)	missense	probably damaging	0.99
R5133:Golgb1	UTSW	16	36,891,457 (GRCm38)	missense	possibly damaging	0.75
R5143:Golgb1	UTSW	16	36,898,689 (GRCm38)	missense	probably benign	0.09
R5185:Golgb1	UTSW	16	36,875,141 (GRCm38)	unclassified	probably benign
R5188:Golgb1	UTSW	16	36,918,465 (GRCm38)	missense	probably benign	0.13
R5260:Golgb1	UTSW	16	36,913,141 (GRCm38)	missense	probably benign	0.00
R5297:Golgb1	UTSW	16	36,875,616 (GRCm38)	intron	probably benign
R5386:Golgb1	UTSW	16	36,912,315 (GRCm38)	nonsense	probably null
R5438:Golgb1	UTSW	16	36,900,508 (GRCm38)	missense	probably benign	0.15
R5439:Golgb1	UTSW	16	36,900,508 (GRCm38)	missense	probably benign	0.15
R5494:Golgb1	UTSW	16	36,928,683 (GRCm38)	missense	possibly damaging	0.67
R5592:Golgb1	UTSW	16	36,925,763 (GRCm38)	missense	probably benign	0.02
R5740:Golgb1	UTSW	16	36,919,000 (GRCm38)	missense	probably damaging	0.99
R5862:Golgb1	UTSW	16	36,926,091 (GRCm38)	splice site	silent
R5928:Golgb1	UTSW	16	36,911,987 (GRCm38)	missense	probably damaging	1.00
R6009:Golgb1	UTSW	16	36,914,959 (GRCm38)	missense	probably damaging	1.00
R6062:Golgb1	UTSW	16	36,914,671 (GRCm38)	missense	possibly damaging	0.89
R6102:Golgb1	UTSW	16	36,912,865 (GRCm38)	missense	probably damaging	1.00
R6198:Golgb1	UTSW	16	36,893,395 (GRCm38)	missense	probably damaging	1.00
R6253:Golgb1	UTSW	16	36,915,622 (GRCm38)	missense	possibly damaging	0.77
R6254:Golgb1	UTSW	16	36,913,978 (GRCm38)	missense	probably damaging	0.99
R6321:Golgb1	UTSW	16	36,918,197 (GRCm38)	nonsense	probably null
R6700:Golgb1	UTSW	16	36,875,584 (GRCm38)	intron	probably benign
R6870:Golgb1	UTSW	16	36,918,203 (GRCm38)	missense	probably damaging	1.00
R6882:Golgb1	UTSW	16	36,913,990 (GRCm38)	missense	probably benign
R6944:Golgb1	UTSW	16	36,912,113 (GRCm38)	missense	probably benign
R7108:Golgb1	UTSW	16	36,913,721 (GRCm38)	missense	probably benign	0.01
R7124:Golgb1	UTSW	16	36,913,673 (GRCm38)	missense	probably benign	0.01
R7125:Golgb1	UTSW	16	36,917,963 (GRCm38)	missense	possibly damaging	0.85
R7187:Golgb1	UTSW	16	36,916,150 (GRCm38)	missense	probably benign	0.43
R7205:Golgb1	UTSW	16	36,875,301 (GRCm38)	missense	unknown
R7206:Golgb1	UTSW	16	36,913,749 (GRCm38)	missense	probably benign	0.41
R7233:Golgb1	UTSW	16	36,914,758 (GRCm38)	missense	possibly damaging	0.91
R7320:Golgb1	UTSW	16	36,915,951 (GRCm38)	nonsense	probably null
R7367:Golgb1	UTSW	16	36,898,546 (GRCm38)	missense	probably benign	0.00
R7408:Golgb1	UTSW	16	36,898,547 (GRCm38)	missense	probably damaging	0.98
R7419:Golgb1	UTSW	16	36,912,919 (GRCm38)	missense	possibly damaging	0.95
R7556:Golgb1	UTSW	16	36,915,793 (GRCm38)	missense	probably benign	0.03
R7599:Golgb1	UTSW	16	36,875,396 (GRCm38)	missense	unknown
R7673:Golgb1	UTSW	16	36,913,669 (GRCm38)	missense	probably benign	0.05
R7789:Golgb1	UTSW	16	36,875,399 (GRCm38)	missense	unknown
R7792:Golgb1	UTSW	16	36,918,730 (GRCm38)	missense	probably benign	0.43
R7830:Golgb1	UTSW	16	36,898,721 (GRCm38)	missense	possibly damaging	0.93
R7847:Golgb1	UTSW	16	36,931,920 (GRCm38)	missense	probably damaging	1.00
R7905:Golgb1	UTSW	16	36,913,685 (GRCm38)	missense	probably benign
R7944:Golgb1	UTSW	16	36,914,104 (GRCm38)	missense	probably benign	0.02
R7945:Golgb1	UTSW	16	36,914,104 (GRCm38)	missense	probably benign	0.02
R7950:Golgb1	UTSW	16	36,915,424 (GRCm38)	missense	probably benign	0.13
R8040:Golgb1	UTSW	16	36,913,479 (GRCm38)	missense	possibly damaging	0.85
R8077:Golgb1	UTSW	16	36,918,633 (GRCm38)	missense	probably damaging	0.99
R8181:Golgb1	UTSW	16	36,916,830 (GRCm38)	missense	probably damaging	1.00
R8370:Golgb1	UTSW	16	36,912,317 (GRCm38)	missense	probably benign	0.00
R8684:Golgb1	UTSW	16	36,914,402 (GRCm38)	missense	possibly damaging	0.92
R8725:Golgb1	UTSW	16	36,919,201 (GRCm38)	missense	probably damaging	1.00
R8727:Golgb1	UTSW	16	36,919,201 (GRCm38)	missense	probably damaging	1.00
R8738:Golgb1	UTSW	16	36,916,313 (GRCm38)	missense	probably damaging	1.00
R8785:Golgb1	UTSW	16	36,919,744 (GRCm38)	missense	probably damaging	0.99
R8824:Golgb1	UTSW	16	36,915,689 (GRCm38)	missense	probably benign
R8825:Golgb1	UTSW	16	36,919,447 (GRCm38)	missense	probably benign	0.00
R8940:Golgb1	UTSW	16	36,916,397 (GRCm38)	missense	probably damaging	1.00
R8962:Golgb1	UTSW	16	36,913,616 (GRCm38)	missense	probably damaging	1.00
R9245:Golgb1	UTSW	16	36,918,819 (GRCm38)	nonsense	probably null
R9365:Golgb1	UTSW	16	36,915,762 (GRCm38)	missense	probably damaging	1.00
R9612:Golgb1	UTSW	16	36,919,605 (GRCm38)	missense	probably benign	0.41
R9620:Golgb1	UTSW	16	36,919,449 (GRCm38)	missense	probably benign
R9691:Golgb1	UTSW	16	36,898,634 (GRCm38)	missense	probably damaging	1.00
R9747:Golgb1	UTSW	16	36,893,407 (GRCm38)	missense	probably damaging	1.00
V1662:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
X0067:Golgb1	UTSW	16	36,914,303 (GRCm38)	nonsense	probably null
Z1088:Golgb1	UTSW	16	36,919,742 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTCTGGAGAAGGCATTTTGGAG -3'
(R):5'- AGCCTCTGAAAGCTCATGGG -3'

Sequencing Primer
(F):5'- GAGAAGGCATTTTGGAGATTCTC -3'
(R):5'- AGCTCATGGGCTTTTATACCCTG -3'

Posted On

2015-02-05