Incidental Mutation 'R3104:Dgkg'
ID 262976
Institutional Source Beutler Lab
Gene Symbol Dgkg
Ensembl Gene ENSMUSG00000022861
Gene Name diacylglycerol kinase, gamma
Synonyms 2900055E17Rik, E430001K23Rik, Dagk3
MMRRC Submission 040578-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.100) question?
Stock # R3104 (G1)
Quality Score 225
Status Validated
Chromosome 16
Chromosomal Location 22287211-22475971 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 22394091 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Serine at position 321 (T321S)
Ref Sequence ENSEMBL: ENSMUSP00000023578 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000023578] [ENSMUST00000089925] [ENSMUST00000137311]
AlphaFold Q91WG7
Predicted Effect probably damaging
Transcript: ENSMUST00000023578
AA Change: T321S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000023578
Gene: ENSMUSG00000022861
AA Change: T321S

DomainStartEndE-ValueType
Pfam:DAG_kinase_N 2 172 9.3e-66 PFAM
EFh 176 204 8.13e-2 SMART
EFh 221 249 1.07e-1 SMART
C1 269 318 1.23e-10 SMART
DAGKc 392 516 5.29e-65 SMART
DAGKa 536 710 1.25e-116 SMART
low complexity region 735 748 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000089925
AA Change: T321S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000087371
Gene: ENSMUSG00000022861
AA Change: T321S

DomainStartEndE-ValueType
Pfam:DAG_kinase_N 5 172 3.9e-54 PFAM
EFh 176 204 8.13e-2 SMART
EFh 221 249 1.07e-1 SMART
C1 269 318 1.23e-10 SMART
C1 334 380 3.35e-3 SMART
DAGKc 431 555 5.29e-65 SMART
DAGKa 575 749 1.25e-116 SMART
low complexity region 774 787 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000137311
AA Change: T321S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000114441
Gene: ENSMUSG00000022861
AA Change: T321S

DomainStartEndE-ValueType
Pfam:DAG_kinase_N 2 172 3.8e-67 PFAM
EFh 176 204 8.13e-2 SMART
EFh 221 249 1.07e-1 SMART
C1 269 318 1.23e-10 SMART
Meta Mutation Damage Score 0.3121 question?
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.4%
  • 10x: 96.9%
  • 20x: 93.8%
Validation Efficiency 100% (57/57)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 54 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ash1l T A 3: 88,961,693 (GRCm39) V2355E probably damaging Het
Baz2a AGCGGCGGTACTTGCGGG AG 10: 127,960,946 (GRCm39) probably null Het
Bmp4 G A 14: 46,623,438 (GRCm39) A36V probably benign Het
Ccdc191 T C 16: 43,751,573 (GRCm39) F301S probably damaging Het
Cdh9 T C 15: 16,855,900 (GRCm39) S647P probably damaging Het
Cntln C T 4: 84,875,406 (GRCm39) T280M possibly damaging Het
Coch A G 12: 51,650,204 (GRCm39) T398A probably benign Het
Col6a3 T C 1: 90,744,024 (GRCm39) R515G probably damaging Het
Csmd1 A G 8: 17,077,247 (GRCm39) Y137H probably damaging Het
Ctnnal1 G A 4: 56,813,246 (GRCm39) L662F probably benign Het
Cyp19a1 T C 9: 54,094,083 (GRCm39) I60V probably benign Het
Cyp2c68 C T 19: 39,722,757 (GRCm39) V264I probably benign Het
Dbx1 A T 7: 49,286,417 (GRCm39) L16H probably damaging Het
Dnah7c T A 1: 46,837,439 (GRCm39) Y3951N probably damaging Het
Emc10 G A 7: 44,142,616 (GRCm39) R109W probably damaging Het
Fam124b T A 1: 80,190,748 (GRCm39) I212F probably damaging Het
Fam187b A G 7: 30,676,665 (GRCm39) D58G probably benign Het
Galnt4 T C 10: 98,945,243 (GRCm39) Y323H probably benign Het
Gfpt1 A G 6: 87,034,628 (GRCm39) D142G probably benign Het
Gm5174 A G 10: 86,492,519 (GRCm39) noncoding transcript Het
Gtf2ird2 G A 5: 134,237,756 (GRCm39) D278N probably benign Het
Herc2 T A 7: 55,785,103 (GRCm39) D1480E probably benign Het
Hnf4g T G 3: 3,717,916 (GRCm39) S388R probably benign Het
Il1rap A G 16: 26,541,502 (GRCm39) E581G probably benign Het
Itpr2 A T 6: 146,214,335 (GRCm39) probably null Het
Lgr6 A G 1: 134,928,210 (GRCm39) probably null Het
Lmod2 A C 6: 24,604,471 (GRCm39) K482T probably damaging Het
Magi3 A G 3: 103,958,636 (GRCm39) V483A probably damaging Het
Ncam2 A G 16: 81,262,598 (GRCm39) probably benign Het
Nphs1 C A 7: 30,166,965 (GRCm39) S724* probably null Het
Or5t9 T C 2: 86,660,035 (GRCm39) M313T probably benign Het
Osgep T C 14: 51,154,286 (GRCm39) T225A probably benign Het
Pcdhgc5 A G 18: 37,954,727 (GRCm39) E667G possibly damaging Het
Plce1 C T 19: 38,608,963 (GRCm39) P424L probably benign Het
Plekhg5 C T 4: 152,196,635 (GRCm39) T694M probably damaging Het
Prkag2 T A 5: 25,076,067 (GRCm39) K233* probably null Het
Prune2 T A 19: 17,096,520 (GRCm39) S675T probably damaging Het
Sars1 C T 3: 108,336,621 (GRCm39) R302H probably damaging Het
Sfmbt1 G A 14: 30,539,753 (GRCm39) C847Y probably damaging Het
Sparcl1 T C 5: 104,241,203 (GRCm39) T74A probably benign Het
Sppl2b A G 10: 80,703,325 (GRCm39) E529G probably benign Het
Stradb C A 1: 59,031,450 (GRCm39) H212Q possibly damaging Het
Tkfc G T 19: 10,574,357 (GRCm39) C198* probably null Het
Tm4sf4 C T 3: 57,345,043 (GRCm39) R150C possibly damaging Het
Tmem212 T C 3: 27,939,019 (GRCm39) S156G probably damaging Het
Tmem51 T C 4: 141,765,035 (GRCm39) N8D probably damaging Het
Tmigd1 A G 11: 76,801,124 (GRCm39) T204A possibly damaging Het
Tsga10 G A 1: 37,840,872 (GRCm39) L445F probably damaging Het
Unc80 G A 1: 66,662,450 (GRCm39) V1768I probably benign Het
Urb1 C T 16: 90,592,331 (GRCm39) V310I probably damaging Het
Usp29 T A 7: 6,965,052 (GRCm39) C298* probably null Het
Usp8 T C 2: 126,600,432 (GRCm39) V1050A probably damaging Het
Vmn1r38 T C 6: 66,753,430 (GRCm39) T229A probably benign Het
Yes1 T C 5: 32,810,515 (GRCm39) S195P probably damaging Het
Other mutations in Dgkg
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00592:Dgkg APN 16 22,298,112 (GRCm39) splice site probably benign
IGL01347:Dgkg APN 16 22,419,340 (GRCm39) missense probably benign 0.13
IGL02313:Dgkg APN 16 22,388,980 (GRCm39) splice site probably benign
IGL02498:Dgkg APN 16 22,367,441 (GRCm39) missense probably damaging 1.00
IGL02954:Dgkg APN 16 22,441,003 (GRCm39) missense probably benign 0.00
IGL03103:Dgkg APN 16 22,399,275 (GRCm39) missense probably damaging 1.00
R0014:Dgkg UTSW 16 22,384,114 (GRCm39) splice site probably null
R0636:Dgkg UTSW 16 22,398,479 (GRCm39) splice site probably benign
R0666:Dgkg UTSW 16 22,381,480 (GRCm39) missense probably damaging 1.00
R1056:Dgkg UTSW 16 22,419,291 (GRCm39) missense probably damaging 1.00
R1495:Dgkg UTSW 16 22,319,129 (GRCm39) missense probably damaging 1.00
R1603:Dgkg UTSW 16 22,388,909 (GRCm39) splice site probably benign
R1993:Dgkg UTSW 16 22,419,344 (GRCm39) missense probably damaging 1.00
R2073:Dgkg UTSW 16 22,384,067 (GRCm39) missense probably damaging 0.96
R2192:Dgkg UTSW 16 22,407,049 (GRCm39) missense probably damaging 0.98
R2251:Dgkg UTSW 16 22,441,010 (GRCm39) start codon destroyed probably null 0.98
R2252:Dgkg UTSW 16 22,441,010 (GRCm39) start codon destroyed probably null 0.98
R3105:Dgkg UTSW 16 22,394,091 (GRCm39) missense probably damaging 1.00
R3106:Dgkg UTSW 16 22,394,091 (GRCm39) missense probably damaging 1.00
R4804:Dgkg UTSW 16 22,393,943 (GRCm39) intron probably benign
R5272:Dgkg UTSW 16 22,407,044 (GRCm39) splice site probably null
R5364:Dgkg UTSW 16 22,419,211 (GRCm39) missense probably benign 0.03
R5417:Dgkg UTSW 16 22,407,081 (GRCm39) missense possibly damaging 0.50
R5677:Dgkg UTSW 16 22,388,921 (GRCm39) missense probably benign 0.00
R5839:Dgkg UTSW 16 22,385,244 (GRCm39) missense possibly damaging 0.91
R5931:Dgkg UTSW 16 22,376,788 (GRCm39) nonsense probably null
R6313:Dgkg UTSW 16 22,338,311 (GRCm39) missense probably damaging 0.96
R7017:Dgkg UTSW 16 22,391,463 (GRCm39) missense probably benign 0.31
R7135:Dgkg UTSW 16 22,319,132 (GRCm39) missense probably damaging 1.00
R7326:Dgkg UTSW 16 22,367,440 (GRCm39) missense probably damaging 1.00
R7476:Dgkg UTSW 16 22,441,054 (GRCm39) start gained probably benign
R7812:Dgkg UTSW 16 22,385,165 (GRCm39) missense probably damaging 1.00
R7971:Dgkg UTSW 16 22,388,966 (GRCm39) nonsense probably null
R8064:Dgkg UTSW 16 22,399,344 (GRCm39) frame shift probably null
R8122:Dgkg UTSW 16 22,385,295 (GRCm39) splice site probably null
R8825:Dgkg UTSW 16 22,381,519 (GRCm39) missense probably benign 0.13
R9049:Dgkg UTSW 16 22,419,338 (GRCm39) missense probably benign 0.01
R9308:Dgkg UTSW 16 22,429,528 (GRCm39) critical splice donor site probably null
R9352:Dgkg UTSW 16 22,398,581 (GRCm39) missense probably damaging 0.99
R9433:Dgkg UTSW 16 22,384,065 (GRCm39) missense probably damaging 1.00
R9545:Dgkg UTSW 16 22,385,168 (GRCm39) missense possibly damaging 0.48
R9606:Dgkg UTSW 16 22,441,011 (GRCm39) start codon destroyed probably null 0.51
R9623:Dgkg UTSW 16 22,385,194 (GRCm39) missense
R9634:Dgkg UTSW 16 22,338,387 (GRCm39) critical splice acceptor site probably null
R9765:Dgkg UTSW 16 22,298,157 (GRCm39) missense possibly damaging 0.70
Z1088:Dgkg UTSW 16 22,391,436 (GRCm39) missense probably benign 0.31
Z1088:Dgkg UTSW 16 22,288,078 (GRCm39) missense probably damaging 0.96
Z1176:Dgkg UTSW 16 22,407,148 (GRCm39) missense probably benign 0.00
Z1177:Dgkg UTSW 16 22,376,834 (GRCm39) missense probably benign 0.22
Predicted Primers PCR Primer
(F):5'- CTTCAGGGAGGTGCTGAATG -3'
(R):5'- AGTGTCAGGTTTCTGCTAAGCC -3'

Sequencing Primer
(F):5'- ATTGATGGATGAGTGTAATGGCTC -3'
(R):5'- TTTCTGCTAAGCCAGGCCAG -3'
Posted On 2015-02-05