Incidental Mutation 'R3105:Dgkg'
ID 263020
Institutional Source Beutler Lab
Gene Symbol Dgkg
Ensembl Gene ENSMUSG00000022861
Gene Name diacylglycerol kinase, gamma
Synonyms 2900055E17Rik, E430001K23Rik, Dagk3
MMRRC Submission 040579-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.100) question?
Stock # R3105 (G1)
Quality Score 225
Status Validated
Chromosome 16
Chromosomal Location 22287211-22475971 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 22394091 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Serine at position 321 (T321S)
Ref Sequence ENSEMBL: ENSMUSP00000023578 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000023578] [ENSMUST00000089925] [ENSMUST00000137311]
AlphaFold Q91WG7
Predicted Effect probably damaging
Transcript: ENSMUST00000023578
AA Change: T321S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000023578
Gene: ENSMUSG00000022861
AA Change: T321S

DomainStartEndE-ValueType
Pfam:DAG_kinase_N 2 172 9.3e-66 PFAM
EFh 176 204 8.13e-2 SMART
EFh 221 249 1.07e-1 SMART
C1 269 318 1.23e-10 SMART
DAGKc 392 516 5.29e-65 SMART
DAGKa 536 710 1.25e-116 SMART
low complexity region 735 748 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000089925
AA Change: T321S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000087371
Gene: ENSMUSG00000022861
AA Change: T321S

DomainStartEndE-ValueType
Pfam:DAG_kinase_N 5 172 3.9e-54 PFAM
EFh 176 204 8.13e-2 SMART
EFh 221 249 1.07e-1 SMART
C1 269 318 1.23e-10 SMART
C1 334 380 3.35e-3 SMART
DAGKc 431 555 5.29e-65 SMART
DAGKa 575 749 1.25e-116 SMART
low complexity region 774 787 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000137311
AA Change: T321S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000114441
Gene: ENSMUSG00000022861
AA Change: T321S

DomainStartEndE-ValueType
Pfam:DAG_kinase_N 2 172 3.8e-67 PFAM
EFh 176 204 8.13e-2 SMART
EFh 221 249 1.07e-1 SMART
C1 269 318 1.23e-10 SMART
Meta Mutation Damage Score 0.3121 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.6%
  • 10x: 97.4%
  • 20x: 95.7%
Validation Efficiency 100% (44/44)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb6 T C 1: 75,151,687 (GRCm39) probably benign Het
Abhd13 T C 8: 10,037,931 (GRCm39) V176A probably benign Het
Alpk2 A T 18: 65,483,281 (GRCm39) S242R possibly damaging Het
Baz2a AGCGGCGGTACTTGCGGG AG 10: 127,960,946 (GRCm39) probably null Het
Ccdc163 C A 4: 116,566,697 (GRCm39) Q58K probably benign Het
Ccdc191 T C 16: 43,751,573 (GRCm39) F301S probably damaging Het
Clca4b A T 3: 144,622,432 (GRCm39) N544K probably benign Het
Col6a3 T C 1: 90,744,024 (GRCm39) R515G probably damaging Het
Dip2b C A 15: 100,040,018 (GRCm39) C138* probably null Het
Dnaja2 G A 8: 86,281,857 (GRCm39) T7M probably damaging Het
Ezh1 A C 11: 101,086,468 (GRCm39) C575W probably damaging Het
F11 T A 8: 45,698,754 (GRCm39) Q463H probably damaging Het
Gfpt1 A G 6: 87,034,628 (GRCm39) D142G probably benign Het
Gm8730 T C 8: 103,591,895 (GRCm39) noncoding transcript Het
Grm1 A G 10: 10,955,601 (GRCm39) S228P probably benign Het
Hnf4g T G 3: 3,717,916 (GRCm39) S388R probably benign Het
Il1rap A G 16: 26,541,502 (GRCm39) E581G probably benign Het
Lrp4 T C 2: 91,331,394 (GRCm39) Y1585H probably benign Het
Magi3 A G 3: 103,958,636 (GRCm39) V483A probably damaging Het
Map2 T C 1: 66,472,756 (GRCm39) probably null Het
Or4a78 T C 2: 89,497,302 (GRCm39) I309M probably benign Het
Osgep T C 14: 51,154,286 (GRCm39) T225A probably benign Het
Otof A G 5: 30,539,145 (GRCm39) Y1090H probably benign Het
Pgr T A 9: 8,958,397 (GRCm39) F801L probably benign Het
Pomk A C 8: 26,472,942 (GRCm39) L337R probably damaging Het
Prune2 T A 19: 17,096,520 (GRCm39) S675T probably damaging Het
Rnpepl1 C T 1: 92,844,102 (GRCm39) L278F probably damaging Het
Sars1 C T 3: 108,336,621 (GRCm39) R302H probably damaging Het
Sbspon T C 1: 15,962,806 (GRCm39) E24G probably benign Het
Sec16a G A 2: 26,328,433 (GRCm39) P1194L probably benign Het
Sfmbt1 G A 14: 30,539,753 (GRCm39) C847Y probably damaging Het
Slc22a29 T C 19: 8,147,337 (GRCm39) T342A probably benign Het
Spaca1 G A 4: 34,028,468 (GRCm39) T283M probably damaging Het
Stradb C A 1: 59,031,450 (GRCm39) H212Q possibly damaging Het
Taf6l C T 19: 8,756,219 (GRCm39) S208N probably damaging Het
Tkfc G T 19: 10,574,357 (GRCm39) C198* probably null Het
Tm4sf4 C T 3: 57,345,043 (GRCm39) R150C possibly damaging Het
Tmem212 T C 3: 27,939,019 (GRCm39) S156G probably damaging Het
Tmigd1 A G 11: 76,801,124 (GRCm39) T204A possibly damaging Het
Tsga10 G A 1: 37,840,872 (GRCm39) L445F probably damaging Het
Urb1 C T 16: 90,592,331 (GRCm39) V310I probably damaging Het
Vmn1r205 T C 13: 22,777,109 (GRCm39) probably benign Het
Other mutations in Dgkg
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00592:Dgkg APN 16 22,298,112 (GRCm39) splice site probably benign
IGL01347:Dgkg APN 16 22,419,340 (GRCm39) missense probably benign 0.13
IGL02313:Dgkg APN 16 22,388,980 (GRCm39) splice site probably benign
IGL02498:Dgkg APN 16 22,367,441 (GRCm39) missense probably damaging 1.00
IGL02954:Dgkg APN 16 22,441,003 (GRCm39) missense probably benign 0.00
IGL03103:Dgkg APN 16 22,399,275 (GRCm39) missense probably damaging 1.00
R0014:Dgkg UTSW 16 22,384,114 (GRCm39) splice site probably null
R0636:Dgkg UTSW 16 22,398,479 (GRCm39) splice site probably benign
R0666:Dgkg UTSW 16 22,381,480 (GRCm39) missense probably damaging 1.00
R1056:Dgkg UTSW 16 22,419,291 (GRCm39) missense probably damaging 1.00
R1495:Dgkg UTSW 16 22,319,129 (GRCm39) missense probably damaging 1.00
R1603:Dgkg UTSW 16 22,388,909 (GRCm39) splice site probably benign
R1993:Dgkg UTSW 16 22,419,344 (GRCm39) missense probably damaging 1.00
R2073:Dgkg UTSW 16 22,384,067 (GRCm39) missense probably damaging 0.96
R2192:Dgkg UTSW 16 22,407,049 (GRCm39) missense probably damaging 0.98
R2251:Dgkg UTSW 16 22,441,010 (GRCm39) start codon destroyed probably null 0.98
R2252:Dgkg UTSW 16 22,441,010 (GRCm39) start codon destroyed probably null 0.98
R3104:Dgkg UTSW 16 22,394,091 (GRCm39) missense probably damaging 1.00
R3106:Dgkg UTSW 16 22,394,091 (GRCm39) missense probably damaging 1.00
R4804:Dgkg UTSW 16 22,393,943 (GRCm39) intron probably benign
R5272:Dgkg UTSW 16 22,407,044 (GRCm39) splice site probably null
R5364:Dgkg UTSW 16 22,419,211 (GRCm39) missense probably benign 0.03
R5417:Dgkg UTSW 16 22,407,081 (GRCm39) missense possibly damaging 0.50
R5677:Dgkg UTSW 16 22,388,921 (GRCm39) missense probably benign 0.00
R5839:Dgkg UTSW 16 22,385,244 (GRCm39) missense possibly damaging 0.91
R5931:Dgkg UTSW 16 22,376,788 (GRCm39) nonsense probably null
R6313:Dgkg UTSW 16 22,338,311 (GRCm39) missense probably damaging 0.96
R7017:Dgkg UTSW 16 22,391,463 (GRCm39) missense probably benign 0.31
R7135:Dgkg UTSW 16 22,319,132 (GRCm39) missense probably damaging 1.00
R7326:Dgkg UTSW 16 22,367,440 (GRCm39) missense probably damaging 1.00
R7476:Dgkg UTSW 16 22,441,054 (GRCm39) start gained probably benign
R7812:Dgkg UTSW 16 22,385,165 (GRCm39) missense probably damaging 1.00
R7971:Dgkg UTSW 16 22,388,966 (GRCm39) nonsense probably null
R8064:Dgkg UTSW 16 22,399,344 (GRCm39) frame shift probably null
R8122:Dgkg UTSW 16 22,385,295 (GRCm39) splice site probably null
R8825:Dgkg UTSW 16 22,381,519 (GRCm39) missense probably benign 0.13
R9049:Dgkg UTSW 16 22,419,338 (GRCm39) missense probably benign 0.01
R9308:Dgkg UTSW 16 22,429,528 (GRCm39) critical splice donor site probably null
R9352:Dgkg UTSW 16 22,398,581 (GRCm39) missense probably damaging 0.99
R9433:Dgkg UTSW 16 22,384,065 (GRCm39) missense probably damaging 1.00
R9545:Dgkg UTSW 16 22,385,168 (GRCm39) missense possibly damaging 0.48
R9606:Dgkg UTSW 16 22,441,011 (GRCm39) start codon destroyed probably null 0.51
R9623:Dgkg UTSW 16 22,385,194 (GRCm39) missense
R9634:Dgkg UTSW 16 22,338,387 (GRCm39) critical splice acceptor site probably null
R9765:Dgkg UTSW 16 22,298,157 (GRCm39) missense possibly damaging 0.70
Z1088:Dgkg UTSW 16 22,391,436 (GRCm39) missense probably benign 0.31
Z1088:Dgkg UTSW 16 22,288,078 (GRCm39) missense probably damaging 0.96
Z1176:Dgkg UTSW 16 22,407,148 (GRCm39) missense probably benign 0.00
Z1177:Dgkg UTSW 16 22,376,834 (GRCm39) missense probably benign 0.22
Predicted Primers PCR Primer
(F):5'- CTTCAGGGAGGTGCTGAATG -3'
(R):5'- CACAGTGTCAGGTTTCTGCTAAG -3'

Sequencing Primer
(F):5'- ATTGATGGATGAGTGTAATGGCTC -3'
(R):5'- TCAGGTTTCTGCTAAGCCAGGC -3'
Posted On 2015-02-05