Mutagenetix > Incidental Mutation

Incidental Mutation 'R3120:Vmn2r14'

263168

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r14

Ensembl Gene

ENSMUSG00000091059

Gene Name

vomeronasal 2, receptor 14

Synonyms

EG231591

MMRRC Submission

040593-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.526) question?

Stock #

R3120 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109362822-109372488 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 109372431 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 20 (W20R)

Ref Sequence

ENSEMBL: ENSMUSP00000128015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170341]

AlphaFold

E9Q759

Predicted Effect

probably null
Transcript: ENSMUST00000170341
AA Change: W20R

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000128015
Gene: ENSMUSG00000091059
AA Change: W20R

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.3e-31	PFAM
Pfam:NCD3G	507	561	1.1e-17	PFAM
Pfam:7tm_3	594	829	1.2e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Bsx	A	G	9: 40,788,908 (GRCm39)	K155R	possibly damaging	Het
Ccdc39	T	C	3: 33,891,987 (GRCm39)	K162E	probably damaging	Het
Crygs	C	T	16: 22,624,301 (GRCm39)	G102D	possibly damaging	Het
Dnah1	G	A	14: 30,988,779 (GRCm39)	R3351*	probably null	Het
Eml1	A	G	12: 108,479,312 (GRCm39)	R362G	probably benign	Het
Fam222b	T	C	11: 78,044,742 (GRCm39)	L101P	probably damaging	Het
Fars2	A	G	13: 36,430,400 (GRCm39)	E276G	probably damaging	Het
Gatad1	G	T	5: 3,691,456 (GRCm39)	Y33*	probably null	Het
Gclc	A	C	9: 77,688,552 (GRCm39)	E219A	possibly damaging	Het
Gm4846	A	G	1: 166,319,117 (GRCm39)	V207A	probably benign	Het
H1f5	A	T	13: 21,964,215 (GRCm39)	S170R	probably benign	Het
Hbq1a	T	C	11: 32,250,472 (GRCm39)	L87P	probably damaging	Het
Magea6	A	T	X: 153,707,291 (GRCm39)	I255N	probably benign	Het
Mfsd12	T	G	10: 81,197,049 (GRCm39)	V206G	probably benign	Het
Mis18bp1	T	C	12: 65,203,762 (GRCm39)		probably null	Het
Nf1	C	A	11: 79,455,725 (GRCm39)	T550K	probably damaging	Het
Nlrp4f	T	C	13: 65,342,530 (GRCm39)	T372A	probably benign	Het
Or5p70	T	C	7: 107,994,930 (GRCm39)	I201T	possibly damaging	Het
Pkdcc	T	C	17: 83,527,466 (GRCm39)	Y215H	probably damaging	Het
Plekha5	A	G	6: 140,537,367 (GRCm39)	T253A	probably benign	Het
Polr2f	T	A	15: 79,028,788 (GRCm39)		probably null	Het
Prph2	C	T	17: 47,234,298 (GRCm39)	A289V	possibly damaging	Het
Ptdss2	C	T	7: 140,732,132 (GRCm39)	H140Y	probably damaging	Het
Rlf	T	A	4: 121,006,680 (GRCm39)	I877L	probably benign	Het
Scgb2b2	T	C	7: 31,003,001 (GRCm39)	L32S	possibly damaging	Het
Sfpq	T	A	4: 126,915,926 (GRCm39)	H239Q	unknown	Het
St6gal2	A	T	17: 55,789,111 (GRCm39)	R48S	probably benign	Het
Sybu	T	C	15: 44,536,355 (GRCm39)	D657G	possibly damaging	Het
Syt15	A	G	14: 33,944,950 (GRCm39)	I166V	probably benign	Het
Taar7f	C	A	10: 23,925,478 (GRCm39)	T24K	probably benign	Het
Tbcd	T	C	11: 121,499,474 (GRCm39)	S1093P	probably damaging	Het
Tfap2c	G	A	2: 172,399,015 (GRCm39)	V396M	possibly damaging	Het
Tnxb	C	T	17: 34,911,329 (GRCm39)	T1544I	possibly damaging	Het
Trim28	A	G	7: 12,762,341 (GRCm39)	T322A	probably damaging	Het
Tubgcp3	G	A	8: 12,707,626 (GRCm39)	A121V	possibly damaging	Het
Zfp551	A	G	7: 12,149,943 (GRCm39)	F489L	possibly damaging	Het

Other mutations in Vmn2r14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Vmn2r14	APN	5	109,364,180 (GRCm39)	nonsense	probably null
IGL01504:Vmn2r14	APN	5	109,369,285 (GRCm39)	missense	probably benign	0.01
IGL01828:Vmn2r14	APN	5	109,372,443 (GRCm39)	missense	possibly damaging	0.71
IGL02093:Vmn2r14	APN	5	109,368,275 (GRCm39)	missense	possibly damaging	0.94
IGL02103:Vmn2r14	APN	5	109,372,349 (GRCm39)	missense	probably damaging	0.96
IGL02123:Vmn2r14	APN	5	109,367,933 (GRCm39)	missense	probably damaging	1.00
IGL02145:Vmn2r14	APN	5	109,368,454 (GRCm39)	nonsense	probably null
IGL02676:Vmn2r14	APN	5	109,367,882 (GRCm39)	missense	probably benign	0.03
IGL02720:Vmn2r14	APN	5	109,369,305 (GRCm39)	missense	probably damaging	1.00
IGL02877:Vmn2r14	APN	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
IGL02974:Vmn2r14	APN	5	109,369,292 (GRCm39)	missense	possibly damaging	0.55
IGL03151:Vmn2r14	APN	5	109,364,260 (GRCm39)	missense	probably damaging	1.00
IGL03297:Vmn2r14	APN	5	109,363,973 (GRCm39)	missense	probably damaging	1.00
IGL03386:Vmn2r14	APN	5	109,368,350 (GRCm39)	missense	possibly damaging	0.90
IGL03394:Vmn2r14	APN	5	109,367,702 (GRCm39)	missense	probably null	0.83
ANU74:Vmn2r14	UTSW	5	109,366,910 (GRCm39)	missense	probably benign	0.00
R0316:Vmn2r14	UTSW	5	109,366,762 (GRCm39)	missense	probably benign	0.07
R0755:Vmn2r14	UTSW	5	109,364,226 (GRCm39)	missense	possibly damaging	0.81
R1219:Vmn2r14	UTSW	5	109,372,440 (GRCm39)	missense	probably benign	0.17
R1321:Vmn2r14	UTSW	5	109,364,117 (GRCm39)	missense	probably benign	0.08
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1509:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R1551:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	probably damaging	1.00
R1628:Vmn2r14	UTSW	5	109,367,838 (GRCm39)	missense	probably benign	0.00
R1668:Vmn2r14	UTSW	5	109,366,913 (GRCm39)	nonsense	probably null
R2013:Vmn2r14	UTSW	5	109,369,109 (GRCm39)	missense	probably benign	0.00
R2201:Vmn2r14	UTSW	5	109,366,698 (GRCm39)	splice site	probably null
R2417:Vmn2r14	UTSW	5	109,372,329 (GRCm39)	missense	probably benign	0.00
R3029:Vmn2r14	UTSW	5	109,363,776 (GRCm39)	missense	probably damaging	1.00
R3729:Vmn2r14	UTSW	5	109,364,095 (GRCm39)	missense	probably damaging	1.00
R3762:Vmn2r14	UTSW	5	109,368,033 (GRCm39)	missense	probably benign	0.02
R3943:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R3944:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R4222:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4224:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4239:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4240:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4782:Vmn2r14	UTSW	5	109,369,370 (GRCm39)	missense	probably benign	0.01
R4832:Vmn2r14	UTSW	5	109,363,976 (GRCm39)	missense	probably damaging	1.00
R4884:Vmn2r14	UTSW	5	109,369,384 (GRCm39)	splice site	probably null
R4896:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5004:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5117:Vmn2r14	UTSW	5	109,363,961 (GRCm39)	missense	probably benign	0.16
R5285:Vmn2r14	UTSW	5	109,365,442 (GRCm39)	missense	probably damaging	0.98
R5413:Vmn2r14	UTSW	5	109,369,154 (GRCm39)	missense	probably benign	0.29
R5569:Vmn2r14	UTSW	5	109,368,261 (GRCm39)	missense	probably benign	0.44
R5701:Vmn2r14	UTSW	5	109,367,816 (GRCm39)	missense	probably damaging	1.00
R5726:Vmn2r14	UTSW	5	109,365,486 (GRCm39)	missense	possibly damaging	0.95
R5763:Vmn2r14	UTSW	5	109,363,724 (GRCm39)	missense	possibly damaging	0.49
R5872:Vmn2r14	UTSW	5	109,369,222 (GRCm39)	missense	probably benign
R5985:Vmn2r14	UTSW	5	109,368,082 (GRCm39)	missense	possibly damaging	0.89
R6268:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	possibly damaging	0.87
R6273:Vmn2r14	UTSW	5	109,369,133 (GRCm39)	missense	probably benign	0.44
R6409:Vmn2r14	UTSW	5	109,364,096 (GRCm39)	missense	probably benign	0.09
R6944:Vmn2r14	UTSW	5	109,364,140 (GRCm39)	missense	probably benign	0.06
R6944:Vmn2r14	UTSW	5	109,363,925 (GRCm39)	missense	probably benign	0.22
R7608:Vmn2r14	UTSW	5	109,369,276 (GRCm39)	missense	probably benign	0.03
R7740:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7768:Vmn2r14	UTSW	5	109,368,086 (GRCm39)	missense	probably benign	0.01
R7804:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7872:Vmn2r14	UTSW	5	109,369,219 (GRCm39)	missense	probably benign	0.02
R7993:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R8006:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8007:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8187:Vmn2r14	UTSW	5	109,368,420 (GRCm39)	missense	probably benign	0.03
R8369:Vmn2r14	UTSW	5	109,369,342 (GRCm39)	missense	probably damaging	1.00
R8463:Vmn2r14	UTSW	5	109,369,340 (GRCm39)	missense	probably benign	0.30
R8968:Vmn2r14	UTSW	5	109,365,533 (GRCm39)	missense	probably benign	0.01
R9008:Vmn2r14	UTSW	5	109,367,893 (GRCm39)	missense	probably benign	0.00
R9030:Vmn2r14	UTSW	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
R9039:Vmn2r14	UTSW	5	109,367,902 (GRCm39)	nonsense	probably null
R9150:Vmn2r14	UTSW	5	109,367,783 (GRCm39)	missense	probably damaging	1.00
R9164:Vmn2r14	UTSW	5	109,364,087 (GRCm39)	missense	probably damaging	1.00
R9216:Vmn2r14	UTSW	5	109,369,112 (GRCm39)	missense	probably benign	0.01
R9225:Vmn2r14	UTSW	5	109,369,288 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r14	UTSW	5	109,368,176 (GRCm39)	missense	possibly damaging	0.89
R9342:Vmn2r14	UTSW	5	109,368,428 (GRCm39)	missense	probably damaging	1.00
R9472:Vmn2r14	UTSW	5	109,367,962 (GRCm39)	missense	probably benign	0.00
R9678:Vmn2r14	UTSW	5	109,364,041 (GRCm39)	missense	probably damaging	1.00
R9774:Vmn2r14	UTSW	5	109,369,126 (GRCm39)	missense	probably benign	0.07
Z1177:Vmn2r14	UTSW	5	109,367,741 (GRCm39)	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- ACCCCACAGTTTGTGTACAC -3'
(R):5'- TATCCAGGGAGTACGACATAGAATG -3'

Sequencing Primer
(F):5'- TACACAACTAAATACATTGGAGCAGG -3'
(R):5'- ATAACTAAGTGGATCTTCAGAGAGC -3'

Posted On

2015-02-05