Mutagenetix > Incidental Mutations

Incidental Mutation 'R3154:Fn1'

263381

Institutional Source

Beutler Lab

Gene Symbol

Fn1

Ensembl Gene

ENSMUSG00000026193

Gene Name

fibronectin 1

Synonyms

Fn-1, Fn

MMRRC Submission

040605-MU

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R3154 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

71585520-71653200 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 71593083 bp

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 2335 (C2335S)

Ref Sequence

ENSEMBL: ENSMUSP00000054499 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055226] [ENSMUST00000186129] [ENSMUST00000187627] [ENSMUST00000187938] [ENSMUST00000188674] [ENSMUST00000188894] [ENSMUST00000189821] [ENSMUST00000190780]

PDB Structure

SOLUTION NMR STRUCTURE OF LINKED CELL ATTACHMENT MODULES OF MOUSE FIBRONECTIN CONTAINING THE RGD AND SYNERGY REGIONS, 20 STRUCTURES [SOLUTION NMR]
SOLUTION NMR STRUCTURE OF LINKED CELL ATTACHMENT MODULES OF MOUSE FIBRONECTIN CONTAINING THE RGD AND SYNERGY REGIONS, 10 STRUCTURES [SOLUTION NMR]

Predicted Effect

probably damaging
Transcript: ENSMUST00000055226
AA Change: C2335S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000054499
Gene: ENSMUSG00000026193
AA Change: C2335S

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1264	1346	4.22e-9	SMART
FN3	1357	1437	9.6e-13	SMART
FN3	1448	1527	1.82e-13	SMART
FN3	1538	1617	6.69e-12	SMART
FN3	1632	1711	2.72e-12	SMART
FN3	1722	1801	8.9e-8	SMART
FN3	1812	1891	1.66e-7	SMART
FN3	1904	1983	4.92e-10	SMART
FN3	1993	2074	3.64e-13	SMART
low complexity region	2148	2165	N/A	INTRINSIC
FN3	2193	2272	2.9e0	SMART
FN1	2296	2340	3.72e-19	SMART
FN1	2341	2383	2.49e-20	SMART
FN1	2385	2425	2.69e-16	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185408

Predicted Effect

probably damaging
Transcript: ENSMUST00000186129
AA Change: C2154S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000141123
Gene: ENSMUSG00000026193
AA Change: C2154S

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1893	3.64e-13	SMART
low complexity region	1967	1984	N/A	INTRINSIC
FN3	2012	2091	2.9e0	SMART
FN1	2115	2159	3.72e-19	SMART
FN1	2160	2202	2.49e-20	SMART
FN1	2204	2244	2.69e-16	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186575

Predicted Effect

probably benign
Transcript: ENSMUST00000187627

SMART Domains

Protein: ENSMUSP00000140955
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
Pfam:fn3	1	49	1.1e-6	PFAM
FN3	58	139	1.7e-15	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000187938
AA Change: C2219S

PolyPhen 2 Score 0.901 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000140975
Gene: ENSMUSG00000026193
AA Change: C2219S

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1983	3.64e-13	SMART
low complexity region	2032	2049	N/A	INTRINSIC
FN3	2077	2156	2.9e0	SMART
FN1	2180	2224	3.72e-19	SMART
FN1	2225	2267	2.49e-20	SMART
FN1	2269	2309	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000188674
AA Change: C2124S

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000140907
Gene: ENSMUSG00000026193
AA Change: C2124S

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1981	6.79e-13	SMART
FN3	1983	2061	1.01e1	SMART
FN1	2085	2129	3.72e-19	SMART
FN1	2130	2172	2.49e-20	SMART
FN1	2174	2214	2.69e-16	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000188894
AA Change: C2244S

PolyPhen 2 Score 0.818 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000140471
Gene: ENSMUSG00000026193
AA Change: C2244S

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1983	3.64e-13	SMART
low complexity region	2057	2074	N/A	INTRINSIC
FN3	2102	2181	2.9e0	SMART
FN1	2205	2249	3.72e-19	SMART
FN1	2250	2292	2.49e-20	SMART
FN1	2294	2334	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000189821
AA Change: C2034S

PolyPhen 2 Score 0.452 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000139702
Gene: ENSMUSG00000026193
AA Change: C2034S

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1891	6.79e-13	SMART
FN3	1893	1971	1.01e1	SMART
FN1	1995	2039	3.72e-19	SMART
FN1	2040	2082	2.49e-20	SMART
FN1	2084	2124	2.69e-16	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000190780
AA Change: C2129S

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000140816
Gene: ENSMUSG00000026193
AA Change: C2129S

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1893	3.64e-13	SMART
low complexity region	1942	1959	N/A	INTRINSIC
FN3	1987	2066	2.9e0	SMART
FN1	2090	2134	3.72e-19	SMART
FN1	2135	2177	2.49e-20	SMART
FN1	2179	2219	2.69e-16	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mutants are defective in mesodermal function. Null mutants are embryonic lethal with major patterning and organizational defects. Conditional mutants live and show increased neuronal apoptosis and susceptibility to induced cerebral ischemia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6820408C15Rik	A	C	2: 152,440,824	N200H	probably damaging	Het
Abca17	T	A	17: 24,328,746	D218V	probably damaging	Het
Ap1b1	T	C	11: 5,023,135	V326A	possibly damaging	Het
Bdp1	A	T	13: 100,049,814	V1710E	probably damaging	Het
Chrna3	C	T	9: 55,016,050	C158Y	probably damaging	Het
Cnnm3	T	A	1: 36,521,222	S608T	probably damaging	Het
Cnot1	T	C	8: 95,744,278	E1314G	possibly damaging	Het
Cobll1	A	T	2: 65,107,050	M406K	probably benign	Het
Cyp2d34	T	C	15: 82,617,566	K248E	probably benign	Het
Dcdc2a	A	C	13: 25,102,357	I125L	probably benign	Het
Dgat1	G	A	15: 76,502,521	L439F	probably benign	Het
Disc1	T	A	8: 125,135,304	S472T	probably damaging	Het
Dnajb3	C	A	1: 88,205,051	V210F	probably benign	Het
Fancd2	A	G	6: 113,593,269	S1394G	possibly damaging	Het
Fasn	A	T	11: 120,807,939	L2475Q	probably damaging	Het
Gpld1	T	C	13: 24,943,620	S2P	unknown	Het
Gpld1	G	T	13: 24,956,163		probably null	Het
Gsc2	G	A	16: 17,914,500	R137W	probably damaging	Het
Gsdme	T	C	6: 50,251,363	R42G	probably damaging	Het
Gtf3c5	T	C	2: 28,579,536	T119A	probably damaging	Het
Hs6st3	T	C	14: 119,868,977	S266P	probably damaging	Het
Htr2a	T	C	14: 74,705,822	F281L	probably benign	Het
Hus1b	T	C	13: 30,947,253	K141R	probably benign	Het
Ireb2	T	C	9: 54,885,946		probably null	Het
Klhdc7a	T	A	4: 139,965,713	Y641F	probably benign	Het
Kri1	T	C	9: 21,281,894	E57G	possibly damaging	Het
Map4	A	G	9: 109,999,792	T82A	probably benign	Het
Mthfr	T	G	4: 148,051,604	M353R	probably benign	Het
Mtus2	A	G	5: 148,303,273		probably benign	Het
Muc5ac	T	A	7: 141,792,736		probably null	Het
Myo15	T	A	11: 60,479,360		probably null	Het
Nynrin	A	T	14: 55,863,587	Q278L	possibly damaging	Het
Pced1b	T	G	15: 97,384,542		probably null	Het
Penk	T	C	4: 4,134,152	D165G	probably damaging	Het
Pfkm	T	A	15: 98,118,209	V90D	probably damaging	Het
Pgk2	T	A	17: 40,208,243	D98V	probably damaging	Het
Psg28	C	A	7: 18,426,423	A283S	possibly damaging	Het
Rgl3	A	G	9: 21,980,774	L338P	probably damaging	Het
Rnf126	A	T	10: 79,761,631	I149N	probably damaging	Het
Ros1	T	C	10: 52,050,981	H2181R	probably benign	Het
Slc38a11	C	T	2: 65,330,335	C305Y	probably damaging	Het
Speg	T	A	1: 75,401,542	V798E	probably damaging	Het
Spesp1	G	A	9: 62,282,094		probably benign	Het
Styk1	A	T	6: 131,310,012	Y84*	probably null	Het
Syt2	T	C	1: 134,741,861	L80P	possibly damaging	Het
Tra2a	C	T	6: 49,245,512		probably benign	Het
Trim10	T	A	17: 36,871,688	C149S	probably damaging	Het
Vmn2r38	T	C	7: 9,094,690	T135A	probably benign	Het
Wipf1	A	G	2: 73,437,490	V188A	possibly damaging	Het
Yipf2	G	A	9: 21,589,901	A67V	probably benign	Het
Zfp759	A	G	13: 67,138,655	E96G	probably benign	Het
Zic3	G	A	X: 58,031,478	V100M	possibly damaging	Het

Other mutations in Fn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Fn1	APN	1	71652873	missense	probably benign	0.28
IGL00402:Fn1	APN	1	71641163	missense	probably damaging	1.00
IGL00946:Fn1	APN	1	71645540	splice site	probably benign
IGL01311:Fn1	APN	1	71628140	missense	probably damaging	1.00
IGL01338:Fn1	APN	1	71626210	missense	probably damaging	0.98
IGL01353:Fn1	APN	1	71586939	missense	probably damaging	1.00
IGL01674:Fn1	APN	1	71606741	missense	probably damaging	1.00
IGL01701:Fn1	APN	1	71629853	splice site	probably benign
IGL01734:Fn1	APN	1	71619485	missense	probably damaging	1.00
IGL01788:Fn1	APN	1	71613837	missense	probably damaging	1.00
IGL02186:Fn1	APN	1	71638534	missense	probably damaging	1.00
IGL02398:Fn1	APN	1	71618670	splice site	probably null
IGL02425:Fn1	APN	1	71641143	splice site	probably benign
IGL02516:Fn1	APN	1	71637323	missense	possibly damaging	0.78
IGL02593:Fn1	APN	1	71602432	missense	probably benign
IGL02651:Fn1	APN	1	71597676	missense	possibly damaging	0.65
IGL02681:Fn1	APN	1	71619482	missense	probably damaging	1.00
IGL02890:Fn1	APN	1	71598372	critical splice donor site	probably null
IGL02929:Fn1	APN	1	71595662	critical splice donor site	probably null
IGL03036:Fn1	APN	1	71629773	missense	probably damaging	1.00
IGL03088:Fn1	APN	1	71614038	splice site	probably null
IGL03142:Fn1	APN	1	71637296	missense	probably damaging	1.00
IGL03172:Fn1	APN	1	71641262	missense	probably damaging	0.99
IGL03184:Fn1	APN	1	71609497	missense	probably benign	0.02
IGL03212:Fn1	APN	1	71641325	nonsense	probably null
IGL03246:Fn1	APN	1	71624296	missense	possibly damaging	0.89
IGL03367:Fn1	APN	1	71597553	missense	probably benign	0.27
PIT4514001:Fn1	UTSW	1	71628456	missense	probably benign	0.01
R0008:Fn1	UTSW	1	71595720	missense	probably damaging	0.98
R0112:Fn1	UTSW	1	71609653	missense	probably damaging	1.00
R0138:Fn1	UTSW	1	71624110	missense	possibly damaging	0.82
R0383:Fn1	UTSW	1	71597685	missense	probably damaging	0.99
R0386:Fn1	UTSW	1	71595786	missense	probably damaging	1.00
R0648:Fn1	UTSW	1	71597585	missense	possibly damaging	0.79
R0684:Fn1	UTSW	1	71595809	splice site	probably null
R1054:Fn1	UTSW	1	71586214	makesense	probably null
R1183:Fn1	UTSW	1	71586245	missense	probably damaging	0.98
R1405:Fn1	UTSW	1	71642078	missense	probably damaging	1.00
R1405:Fn1	UTSW	1	71642078	missense	probably damaging	1.00
R1414:Fn1	UTSW	1	71601303	splice site	probably benign
R1677:Fn1	UTSW	1	71597655	missense	probably benign	0.00
R1773:Fn1	UTSW	1	71637383	missense	probably damaging	1.00
R1830:Fn1	UTSW	1	71624259	missense	probably damaging	1.00
R1987:Fn1	UTSW	1	71651625	missense	probably damaging	1.00
R1989:Fn1	UTSW	1	71651625	missense	probably damaging	1.00
R2068:Fn1	UTSW	1	71600439	missense	probably damaging	1.00
R2113:Fn1	UTSW	1	71626164	missense	probably damaging	1.00
R2145:Fn1	UTSW	1	71606004	missense	probably damaging	1.00
R2246:Fn1	UTSW	1	71628535	missense	probably benign	0.10
R2273:Fn1	UTSW	1	71613943	missense	probably null	1.00
R2274:Fn1	UTSW	1	71613943	missense	probably null	1.00
R2275:Fn1	UTSW	1	71613943	missense	probably null	1.00
R2303:Fn1	UTSW	1	71614036	critical splice acceptor site	probably null
R2379:Fn1	UTSW	1	71649284	nonsense	probably null
R2382:Fn1	UTSW	1	71648119	missense	probably damaging	1.00
R2567:Fn1	UTSW	1	71597736	nonsense	probably null
R2864:Fn1	UTSW	1	71602419	missense	probably damaging	0.99
R3837:Fn1	UTSW	1	71653155	utr 5 prime	probably null
R3844:Fn1	UTSW	1	71609574	missense	possibly damaging	0.61
R3886:Fn1	UTSW	1	71640306	missense	probably damaging	1.00
R3887:Fn1	UTSW	1	71640306	missense	probably damaging	1.00
R3888:Fn1	UTSW	1	71640306	missense	probably damaging	1.00
R3889:Fn1	UTSW	1	71640306	missense	probably damaging	1.00
R3905:Fn1	UTSW	1	71607913	missense	probably damaging	1.00
R3906:Fn1	UTSW	1	71607913	missense	probably damaging	1.00
R3907:Fn1	UTSW	1	71607913	missense	probably damaging	1.00
R3909:Fn1	UTSW	1	71607913	missense	probably damaging	1.00
R4611:Fn1	UTSW	1	71624178	nonsense	probably null
R4724:Fn1	UTSW	1	71648148	critical splice acceptor site	probably null
R4732:Fn1	UTSW	1	71602512	splice site	probably null
R4733:Fn1	UTSW	1	71602512	splice site	probably null
R4756:Fn1	UTSW	1	71590808	missense	probably damaging	1.00
R4809:Fn1	UTSW	1	71652800	intron	probably benign
R4839:Fn1	UTSW	1	71642083	missense	probably damaging	1.00
R4915:Fn1	UTSW	1	71595809	splice site	probably null
R4917:Fn1	UTSW	1	71595809	splice site	probably null
R4918:Fn1	UTSW	1	71595809	splice site	probably null
R5002:Fn1	UTSW	1	71629728	missense	possibly damaging	0.48
R5015:Fn1	UTSW	1	71626177	missense	probably damaging	0.98
R5022:Fn1	UTSW	1	71624179	missense	probably damaging	1.00
R5109:Fn1	UTSW	1	71649235	missense	probably damaging	1.00
R5267:Fn1	UTSW	1	71629704	missense	probably damaging	1.00
R5323:Fn1	UTSW	1	71597432	missense	probably benign	0.09
R5333:Fn1	UTSW	1	71624180	missense	probably damaging	1.00
R5631:Fn1	UTSW	1	71590196	missense	probably damaging	1.00
R5644:Fn1	UTSW	1	71627250	missense	probably damaging	1.00
R5754:Fn1	UTSW	1	71600322	missense	probably damaging	1.00
R5807:Fn1	UTSW	1	71648059	missense	probably damaging	1.00
R6053:Fn1	UTSW	1	71599290	missense	probably damaging	1.00
R6133:Fn1	UTSW	1	71597727	missense	probably damaging	1.00
R6186:Fn1	UTSW	1	71637290	missense	probably damaging	1.00
R6270:Fn1	UTSW	1	71637275	missense	probably damaging	1.00
R6332:Fn1	UTSW	1	71628071	missense	probably benign	0.01
R6431:Fn1	UTSW	1	71647844	intron	probably null
R6571:Fn1	UTSW	1	71626190	missense	probably damaging	1.00
R6596:Fn1	UTSW	1	71609482	missense	probably damaging	1.00
R6862:Fn1	UTSW	1	71613907	missense	probably benign	0.43
R6898:Fn1	UTSW	1	71600413	missense	probably damaging	1.00
R6984:Fn1	UTSW	1	71626079	missense	probably damaging	1.00
R7107:Fn1	UTSW	1	71627249	missense	probably damaging	1.00
R7121:Fn1	UTSW	1	71600538	intron	probably benign
R7127:Fn1	UTSW	1	71597544	missense	probably benign	0.16
R7194:Fn1	UTSW	1	71602323	missense	probably damaging	1.00
R7274:Fn1	UTSW	1	71628113	missense	probably benign
R7285:Fn1	UTSW	1	71637339	missense	probably damaging	1.00
R7426:Fn1	UTSW	1	71649225	missense	probably damaging	1.00
R7453:Fn1	UTSW	1	71590880	missense	probably damaging	1.00
R7508:Fn1	UTSW	1	71597516	missense	probably benign	0.01
R7724:Fn1	UTSW	1	71603735	missense	probably benign	0.02
X0023:Fn1	UTSW	1	71598373	critical splice donor site	probably null
Z1088:Fn1	UTSW	1	71649292	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACACAACTCTTGAAATGCAGTC -3'
(R):5'- TGGCCGTTTCCCCATCAATAAC -3'

Sequencing Primer
(F):5'- CAACTCTTGAAATGCAGTCATATTGC -3'
(R):5'- AACCGTCAATAAAATGTCTTCTCC -3'

Posted On

2015-02-05