Incidental Mutation 'R3158:Med14'
ID 263606
Institutional Source Beutler Lab
Gene Symbol Med14
Ensembl Gene ENSMUSG00000064127
Gene Name mediator complex subunit 14
Synonyms Crsp2, ENSMUSG00000073278, 9930001L01Rik, LOC270579, ORF1, Trap170
MMRRC Submission 040609-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.947) question?
Stock # R3158 (G1)
Quality Score 222
Status Validated
Chromosome X
Chromosomal Location 12541608-12628312 bp(-) (GRCm39)
Type of Mutation splice site
DNA Base Change (assembly) G to A at 12550330 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000111143 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000096495] [ENSMUST00000115481]
AlphaFold A2ABV5
Predicted Effect probably benign
Transcript: ENSMUST00000096495
SMART Domains Protein: ENSMUSP00000094239
Gene: ENSMUSG00000064127

DomainStartEndE-ValueType
low complexity region 13 54 N/A INTRINSIC
Pfam:Med14 55 244 6.7e-63 PFAM
low complexity region 608 621 N/A INTRINSIC
low complexity region 1005 1018 N/A INTRINSIC
low complexity region 1065 1086 N/A INTRINSIC
low complexity region 1346 1361 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000115481
SMART Domains Protein: ENSMUSP00000111143
Gene: ENSMUSG00000064127

DomainStartEndE-ValueType
low complexity region 366 379 N/A INTRINSIC
low complexity region 426 447 N/A INTRINSIC
low complexity region 707 722 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000124053
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.1%
  • 20x: 94.4%
Validation Efficiency 97% (35/36)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]
PHENOTYPE: Male chimeras hemizygous for a gene trapped allele appear normal at E10.5. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 34 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aoc2 A T 11: 101,220,102 (GRCm39) N696I probably damaging Het
Ccr4 G T 9: 114,321,350 (GRCm39) N238K probably benign Het
Cd300e A C 11: 114,952,849 (GRCm39) M1R probably null Het
Cep95 A G 11: 106,700,013 (GRCm39) probably benign Het
Cfap54 T C 10: 92,834,918 (GRCm39) I1096V probably benign Het
Clca4b C A 3: 144,617,878 (GRCm39) V742L probably benign Het
Diaph3 A T 14: 86,893,892 (GRCm39) I39N possibly damaging Het
Dll3 A T 7: 27,993,520 (GRCm39) D566E possibly damaging Het
Dmpk A G 7: 18,826,944 (GRCm39) T579A probably benign Het
E330034G19Rik A T 14: 24,346,965 (GRCm39) Y84F possibly damaging Het
Eya1 G A 1: 14,374,691 (GRCm39) probably benign Het
Fat4 A G 3: 38,944,940 (GRCm39) T1278A possibly damaging Het
Galnt12 A G 4: 47,104,264 (GRCm39) D174G probably damaging Het
Gm20939 T A 17: 95,184,721 (GRCm39) H456Q probably damaging Het
Gm7853 A G 14: 35,811,358 (GRCm39) noncoding transcript Het
Hsd3b5 G A 3: 98,529,375 (GRCm39) A85V probably benign Het
Itga11 A G 9: 62,676,560 (GRCm39) K916R probably benign Het
Kcnh8 GAGACCAACGAGCAGCTGATGCTTCAGA GAGA 17: 53,032,934 (GRCm39) 74 probably benign Het
Krt6a T C 15: 101,599,801 (GRCm39) Y437C probably damaging Het
Lrp5 A G 19: 3,665,849 (GRCm39) S707P probably damaging Het
Mmp11 C T 10: 75,762,948 (GRCm39) probably benign Het
Mtus2 A G 5: 148,168,637 (GRCm39) H950R probably damaging Het
Myo1g G T 11: 6,464,527 (GRCm39) T511K possibly damaging Het
Myo7a A G 7: 97,701,499 (GRCm39) F2154S probably damaging Het
Or11h4 A G 14: 50,974,271 (GRCm39) V116A probably benign Het
Or5b101 G C 19: 13,005,411 (GRCm39) A94G probably benign Het
Or8h8 C T 2: 86,752,950 (GRCm39) E309K probably benign Het
Prss52 G T 14: 64,350,992 (GRCm39) W259L probably damaging Het
Sbk2 G A 7: 4,960,526 (GRCm39) R215* probably null Het
Sectm1a A G 11: 120,959,603 (GRCm39) I175T probably benign Het
Smu1 T A 4: 40,754,529 (GRCm39) R123S possibly damaging Het
Stk3 A G 15: 35,008,387 (GRCm39) S178P possibly damaging Het
Tle6 T C 10: 81,431,038 (GRCm39) probably null Het
Vmn2r37 C T 7: 9,220,713 (GRCm39) M383I probably benign Het
Other mutations in Med14
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00565:Med14 APN X 12,613,003 (GRCm39) splice site probably benign
IGL00670:Med14 APN X 12,620,428 (GRCm39) missense probably damaging 0.98
IGL00895:Med14 APN X 12,547,039 (GRCm39) missense probably damaging 0.99
IGL02434:Med14 APN X 12,612,063 (GRCm39) missense possibly damaging 0.89
IGL03064:Med14 APN X 12,613,742 (GRCm39) missense probably benign 0.04
R0295:Med14 UTSW X 12,551,987 (GRCm39) missense probably damaging 1.00
R2844:Med14 UTSW X 12,550,235 (GRCm39) missense probably benign 0.01
R2860:Med14 UTSW X 12,585,936 (GRCm39) missense probably benign
R2861:Med14 UTSW X 12,585,936 (GRCm39) missense probably benign
R2862:Med14 UTSW X 12,585,936 (GRCm39) missense probably benign
R3157:Med14 UTSW X 12,550,330 (GRCm39) splice site probably benign
R3807:Med14 UTSW X 12,553,416 (GRCm39) missense probably damaging 1.00
X0022:Med14 UTSW X 12,553,380 (GRCm39) missense probably damaging 1.00
Z1088:Med14 UTSW X 12,543,845 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CGCAAAGTGAGTGAGCTAGACC -3'
(R):5'- TGCAGAGTAGCCCTTAGTCC -3'

Sequencing Primer
(F):5'- GTGAGCTAGACCATAAGTACTGTTC -3'
(R):5'- CCAGACCCTTCAGCTAAAAGTG -3'
Posted On 2015-02-05