Mutagenetix > Incidental Mutation

Incidental Mutation 'R3107:Lama2'

263627

Institutional Source

Beutler Lab

Gene Symbol

Lama2

Ensembl Gene

ENSMUSG00000019899

Gene Name

laminin, alpha 2

Synonyms

merosin, mer

MMRRC Submission

040581-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.398) question?

Stock #

R3107 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

26980036-27619758 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 27001235 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 2652 (E2652K)

Ref Sequence

ENSEMBL: ENSMUSP00000090304 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092639]

AlphaFold

no structure available at present

PDB Structure

LAMININ ALPHA 2 CHAIN LG4-5 DOMAIN PAIR [X-RAY DIFFRACTION]
LAMININ ALPHA 2 CHAIN LG4-5 DOMAIN PAIR, CA1 SITE MUTANT [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF THE FIFTH LAMININ G-LIKE MODULE OF THE MOUSE LAMININ ALPHA2 CHAIN [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF THE LG1-3 REGION OF THE LAMININ ALPHA2 CHAIN [X-RAY DIFFRACTION]

Predicted Effect

probably benign
Transcript: ENSMUST00000092639
AA Change: E2652K

PolyPhen 2 Score 0.112 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000090304
Gene: ENSMUSG00000019899
AA Change: E2652K

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LamNT	29	281	5.35e-129	SMART
EGF_Lam	283	337	2.11e-4	SMART
EGF_Lam	340	407	1.59e-8	SMART
EGF_Lam	410	462	5.44e-7	SMART
EGF_Lam	465	511	9.05e-4	SMART
LamB	574	706	2.26e-44	SMART
Pfam:Laminin_EGF	715	745	2.8e-4	PFAM
EGF_Lam	753	800	4.03e-10	SMART
EGF_Lam	803	858	3.01e-9	SMART
EGF_Lam	861	911	1.35e-11	SMART
EGF_Lam	914	960	7.23e-12	SMART
EGF_Lam	963	1007	5.87e-12	SMART
EGF_Lam	1010	1053	1.28e-12	SMART
EGF_Lam	1056	1099	2.37e-7	SMART
EGF_Lam	1102	1159	3.22e-9	SMART
LamB	1225	1360	1.95e-57	SMART
EGF_like	1364	1413	8.13e-1	SMART
EGF_Lam	1416	1462	5.48e-12	SMART
EGF_Lam	1465	1520	1.27e-7	SMART
EGF_Lam	1523	1567	2.4e-8	SMART
Pfam:Laminin_I	1584	1849	2e-92	PFAM
Blast:MA	1881	2113	1e-112	BLAST
LamG	2162	2307	1.28e-25	SMART
LamG	2356	2500	2.2e-33	SMART
LamG	2542	2688	3.31e-28	SMART
low complexity region	2725	2741	N/A	INTRINSIC
LamG	2781	2914	2.25e-39	SMART
LamG	2956	3092	1.53e-32	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000219763
AA Change: E660K

Meta Mutation Damage Score

0.1319

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted and spontaneous mutations exhibit progressive growth retardation, ataxia, muscle atrophy and degeneration, infertility, and premature lethality. Muscle fiber degeneration is evident as early as the first week of life. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930021J03Rik	A	T	19: 29,723,447 (GRCm38)	L814H	probably damaging	Het
Adgrf4	A	T	17: 42,666,867 (GRCm38)	Y528*	probably null	Het
Ankrd26	A	T	6: 118,556,243 (GRCm38)	F198L	probably benign	Het
Arnt2	A	G	7: 84,262,444 (GRCm38)	S607P	possibly damaging	Het
Ccdc17	T	C	4: 116,598,267 (GRCm38)	V269A	probably benign	Het
Cers1	T	A	8: 70,322,636 (GRCm38)	H233Q	probably benign	Het
Cfap54	T	C	10: 92,994,683 (GRCm38)	N1197S	probably benign	Het
Cfb	A	G	17: 34,861,824 (GRCm38)	Y66H	possibly damaging	Het
Clspn	A	G	4: 126,591,659 (GRCm38)	D1247G	probably benign	Het
Cnnm1	T	C	19: 43,441,561 (GRCm38)	C373R	probably damaging	Het
Col19a1	T	C	1: 24,337,936 (GRCm38)	T443A	possibly damaging	Het
Cubn	T	C	2: 13,362,347 (GRCm38)	S1571G	possibly damaging	Het
Ddt	C	T	10: 75,772,763 (GRCm38)	E42K	probably benign	Het
Dmrt2	C	A	19: 25,677,691 (GRCm38)	T218N	probably benign	Het
Dnah7b	G	A	1: 46,352,873 (GRCm38)	G3798E	probably benign	Het
Espl1	A	G	15: 102,312,989 (GRCm38)	I944V	probably damaging	Het
Fam114a2	C	A	11: 57,499,735 (GRCm38)	K317N	probably benign	Het
Fyn	G	C	10: 39,551,455 (GRCm38)	D445H	probably damaging	Het
Gprasp1	A	T	X: 135,799,759 (GRCm38)	M234L	probably benign	Het
Ibtk	T	C	9: 85,710,414 (GRCm38)	Y997C	probably damaging	Het
Il12rb2	A	G	6: 67,360,798 (GRCm38)	V33A	probably damaging	Het
Ints6	T	C	14: 62,760,592 (GRCm38)	T23A	possibly damaging	Het
Itk	C	A	11: 46,327,464 (GRCm38)	G624V	probably benign	Het
Mab21l3	T	A	3: 101,826,796 (GRCm38)	I109F	probably damaging	Het
Mov10	T	C	3: 104,799,724 (GRCm38)	E653G	probably damaging	Het
Olfr916	C	T	9: 38,658,011 (GRCm38)	C127Y	possibly damaging	Het
Plg	T	C	17: 12,384,429 (GRCm38)	V74A	probably benign	Het
Ptprn2	A	G	12: 116,876,180 (GRCm38)	D441G	probably benign	Het
Rbmxl2	G	A	7: 107,210,417 (GRCm38)	G303E	probably damaging	Het
Satb1	A	T	17: 51,782,782 (GRCm38)	Y346N	possibly damaging	Het
Serpina1a	A	T	12: 103,853,841 (GRCm38)	I382N	probably damaging	Het
Slc6a2	C	A	8: 92,961,278 (GRCm38)	Q11K	probably benign	Het
Slc6a20a	A	C	9: 123,641,708 (GRCm38)		probably null	Het
Sorcs1	T	C	19: 50,210,650 (GRCm38)	E825G	possibly damaging	Het
Sval1	C	G	6: 41,955,942 (GRCm38)	P145A	probably damaging	Het
Trhde	C	T	10: 114,592,066 (GRCm38)	E442K	probably damaging	Het
Vmn2r61	A	T	7: 42,267,067 (GRCm38)	D368V	possibly damaging	Het

Other mutations in Lama2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00392:Lama2	APN	10	27,188,265 (GRCm38)	missense	probably benign	0.01
IGL00467:Lama2	APN	10	27,467,197 (GRCm38)	splice site	probably benign
IGL00470:Lama2	APN	10	27,243,742 (GRCm38)	missense	probably benign	0.22
IGL00517:Lama2	APN	10	27,197,330 (GRCm38)	missense	probably benign	0.01
IGL00541:Lama2	APN	10	27,188,306 (GRCm38)	missense	probably benign	0.14
IGL00931:Lama2	APN	10	27,006,776 (GRCm38)	missense	possibly damaging	0.92
IGL00951:Lama2	APN	10	27,030,285 (GRCm38)	missense	probably benign	0.03
IGL00988:Lama2	APN	10	27,369,015 (GRCm38)	nonsense	probably null
IGL01098:Lama2	APN	10	27,031,112 (GRCm38)	missense	possibly damaging	0.66
IGL01152:Lama2	APN	10	27,208,429 (GRCm38)	missense	probably benign	0.00
IGL01293:Lama2	APN	10	27,231,636 (GRCm38)	missense	probably benign	0.38
IGL01338:Lama2	APN	10	27,188,272 (GRCm38)	missense	probably benign	0.13
IGL01609:Lama2	APN	10	27,344,421 (GRCm38)	missense	probably benign	0.03
IGL01643:Lama2	APN	10	27,070,372 (GRCm38)	splice site	probably benign
IGL01675:Lama2	APN	10	27,188,054 (GRCm38)	missense	possibly damaging	0.77
IGL01681:Lama2	APN	10	27,265,045 (GRCm38)	missense	probably benign	0.33
IGL01694:Lama2	APN	10	27,006,742 (GRCm38)	missense	possibly damaging	0.75
IGL01705:Lama2	APN	10	27,189,274 (GRCm38)	splice site	probably benign
IGL01885:Lama2	APN	10	27,105,139 (GRCm38)	nonsense	probably null
IGL01935:Lama2	APN	10	27,422,604 (GRCm38)	missense	probably damaging	0.98
IGL01994:Lama2	APN	10	27,467,203 (GRCm38)	critical splice donor site	probably null
IGL02041:Lama2	APN	10	26,984,326 (GRCm38)	missense	probably damaging	1.00
IGL02067:Lama2	APN	10	27,176,796 (GRCm38)	missense	probably benign	0.02
IGL02097:Lama2	APN	10	27,138,960 (GRCm38)	missense	probably benign	0.09
IGL02179:Lama2	APN	10	27,070,364 (GRCm38)	missense	probably benign	0.01
IGL02268:Lama2	APN	10	27,001,116 (GRCm38)	splice site	probably benign
IGL02302:Lama2	APN	10	27,212,043 (GRCm38)	missense	probably benign	0.06
IGL02363:Lama2	APN	10	27,366,066 (GRCm38)	missense	probably damaging	1.00
IGL02378:Lama2	APN	10	27,043,656 (GRCm38)	missense	probably damaging	0.99
IGL02642:Lama2	APN	10	27,467,273 (GRCm38)	missense	probably damaging	1.00
IGL02676:Lama2	APN	10	27,118,493 (GRCm38)	missense	probably benign	0.00
IGL02695:Lama2	APN	10	27,000,775 (GRCm38)	missense	probably benign
IGL02735:Lama2	APN	10	27,104,128 (GRCm38)	missense	probably damaging	1.00
IGL02794:Lama2	APN	10	27,041,231 (GRCm38)	missense	possibly damaging	0.73
IGL02823:Lama2	APN	10	27,001,145 (GRCm38)	missense	probably damaging	1.00
IGL02869:Lama2	APN	10	27,015,538 (GRCm38)	missense	probably damaging	0.99
IGL02942:Lama2	APN	10	27,041,220 (GRCm38)	missense	probably damaging	1.00
IGL03201:Lama2	APN	10	27,344,570 (GRCm38)	nonsense	probably null
IGL03268:Lama2	APN	10	27,422,653 (GRCm38)	missense	probably damaging	1.00
IGL03288:Lama2	APN	10	27,369,051 (GRCm38)	missense	probably damaging	1.00
IGL03380:Lama2	APN	10	27,050,265 (GRCm38)	missense	probably damaging	1.00
IGL03407:Lama2	APN	10	27,347,021 (GRCm38)	missense	probably damaging	1.00
cowboy	UTSW	10	27,043,643 (GRCm38)	frame shift	probably null
petri	UTSW	10	26,993,398 (GRCm38)	splice site	probably null
PIT4362001:Lama2	UTSW	10	27,369,136 (GRCm38)	missense	probably damaging	1.00
PIT4382001:Lama2	UTSW	10	27,204,905 (GRCm38)	missense	probably damaging	1.00
PIT4431001:Lama2	UTSW	10	27,101,430 (GRCm38)	missense	probably damaging	1.00
R0038:Lama2	UTSW	10	26,986,797 (GRCm38)	missense	probably benign	0.02
R0038:Lama2	UTSW	10	26,986,797 (GRCm38)	missense	probably benign	0.02
R0114:Lama2	UTSW	10	26,993,068 (GRCm38)	nonsense	probably null
R0142:Lama2	UTSW	10	27,187,845 (GRCm38)	missense	probably benign
R0313:Lama2	UTSW	10	26,993,398 (GRCm38)	splice site	probably null
R0376:Lama2	UTSW	10	27,015,546 (GRCm38)	missense	possibly damaging	0.68
R0412:Lama2	UTSW	10	27,190,625 (GRCm38)	missense	possibly damaging	0.58
R0472:Lama2	UTSW	10	26,990,867 (GRCm38)	missense	probably damaging	1.00
R0607:Lama2	UTSW	10	27,189,131 (GRCm38)	missense	probably benign	0.34
R0648:Lama2	UTSW	10	26,989,376 (GRCm38)	missense	probably benign	0.00
R0667:Lama2	UTSW	10	27,344,410 (GRCm38)	splice site	probably null
R0760:Lama2	UTSW	10	27,044,433 (GRCm38)	critical splice donor site	probably null
R1240:Lama2	UTSW	10	27,041,124 (GRCm38)	missense	probably damaging	1.00
R1385:Lama2	UTSW	10	27,224,043 (GRCm38)	missense	probably benign	0.11
R1433:Lama2	UTSW	10	27,187,754 (GRCm38)	missense	probably damaging	1.00
R1434:Lama2	UTSW	10	27,208,370 (GRCm38)	missense	probably damaging	1.00
R1574:Lama2	UTSW	10	27,324,754 (GRCm38)	missense	possibly damaging	0.65
R1574:Lama2	UTSW	10	27,324,754 (GRCm38)	missense	possibly damaging	0.65
R1645:Lama2	UTSW	10	27,368,985 (GRCm38)	missense	probably damaging	1.00
R1702:Lama2	UTSW	10	27,190,529 (GRCm38)	missense	probably benign
R1703:Lama2	UTSW	10	27,266,671 (GRCm38)	missense	probably damaging	1.00
R1769:Lama2	UTSW	10	27,208,407 (GRCm38)	missense	probably benign
R1769:Lama2	UTSW	10	27,208,406 (GRCm38)	missense	probably damaging	1.00
R1846:Lama2	UTSW	10	27,212,096 (GRCm38)	missense	probably damaging	1.00
R1859:Lama2	UTSW	10	27,031,082 (GRCm38)	missense	possibly damaging	0.51
R1871:Lama2	UTSW	10	26,984,494 (GRCm38)	missense	probably damaging	1.00
R1903:Lama2	UTSW	10	27,188,399 (GRCm38)	missense	probably damaging	1.00
R1906:Lama2	UTSW	10	27,056,527 (GRCm38)	critical splice donor site	probably null
R1958:Lama2	UTSW	10	26,981,598 (GRCm38)	missense	probably damaging	0.97
R1959:Lama2	UTSW	10	27,422,618 (GRCm38)	missense	probably damaging	1.00
R1977:Lama2	UTSW	10	26,990,800 (GRCm38)	splice site	probably null
R2063:Lama2	UTSW	10	27,164,926 (GRCm38)	missense	probably damaging	1.00
R2079:Lama2	UTSW	10	27,369,053 (GRCm38)	missense	probably damaging	0.99
R2085:Lama2	UTSW	10	27,204,841 (GRCm38)	nonsense	probably null
R2125:Lama2	UTSW	10	27,044,453 (GRCm38)	nonsense	probably null
R2140:Lama2	UTSW	10	27,054,694 (GRCm38)	splice site	probably null
R2219:Lama2	UTSW	10	27,043,569 (GRCm38)	missense	probably damaging	0.99
R2259:Lama2	UTSW	10	27,031,127 (GRCm38)	missense	probably benign	0.00
R2265:Lama2	UTSW	10	26,992,936 (GRCm38)	missense	probably damaging	1.00
R2266:Lama2	UTSW	10	26,986,797 (GRCm38)	missense	probably benign	0.02
R2267:Lama2	UTSW	10	26,992,936 (GRCm38)	missense	probably damaging	1.00
R2268:Lama2	UTSW	10	26,992,936 (GRCm38)	missense	probably damaging	1.00
R2269:Lama2	UTSW	10	26,992,936 (GRCm38)	missense	probably damaging	1.00
R2862:Lama2	UTSW	10	27,422,612 (GRCm38)	nonsense	probably null
R2912:Lama2	UTSW	10	27,000,803 (GRCm38)	missense	probably benign
R2999:Lama2	UTSW	10	26,989,421 (GRCm38)	missense	probably benign	0.18
R3034:Lama2	UTSW	10	27,001,235 (GRCm38)	missense	probably benign	0.11
R3081:Lama2	UTSW	10	27,001,235 (GRCm38)	missense	probably benign	0.11
R3109:Lama2	UTSW	10	27,001,235 (GRCm38)	missense	probably benign	0.11
R3436:Lama2	UTSW	10	27,001,235 (GRCm38)	missense	probably benign	0.11
R3437:Lama2	UTSW	10	27,001,235 (GRCm38)	missense	probably benign	0.11
R3706:Lama2	UTSW	10	27,138,996 (GRCm38)	missense	probably damaging	1.00
R3780:Lama2	UTSW	10	27,459,339 (GRCm38)	missense	probably damaging	1.00
R3807:Lama2	UTSW	10	27,190,665 (GRCm38)	frame shift	probably null
R3919:Lama2	UTSW	10	27,118,505 (GRCm38)	missense	probably damaging	1.00
R4014:Lama2	UTSW	10	26,984,376 (GRCm38)	missense	probably damaging	1.00
R4131:Lama2	UTSW	10	27,041,174 (GRCm38)	missense	probably benign	0.00
R4190:Lama2	UTSW	10	27,266,664 (GRCm38)	missense	probably damaging	0.96
R4273:Lama2	UTSW	10	27,347,054 (GRCm38)	missense	probably damaging	1.00
R4358:Lama2	UTSW	10	26,984,493 (GRCm38)	missense	probably damaging	1.00
R4407:Lama2	UTSW	10	27,212,128 (GRCm38)	small deletion	probably benign
R4415:Lama2	UTSW	10	26,989,344 (GRCm38)	nonsense	probably null
R4426:Lama2	UTSW	10	27,422,558 (GRCm38)	missense	probably damaging	1.00
R4590:Lama2	UTSW	10	26,989,414 (GRCm38)	missense	probably benign	0.00
R4615:Lama2	UTSW	10	26,981,524 (GRCm38)	missense	probably damaging	0.99
R4736:Lama2	UTSW	10	27,204,929 (GRCm38)	missense	probably damaging	1.00
R4754:Lama2	UTSW	10	27,118,531 (GRCm38)	missense	possibly damaging	0.58
R4791:Lama2	UTSW	10	27,467,271 (GRCm38)	missense	probably damaging	1.00
R4834:Lama2	UTSW	10	27,006,749 (GRCm38)	missense	probably benign	0.30
R4856:Lama2	UTSW	10	27,043,643 (GRCm38)	frame shift	probably null
R4858:Lama2	UTSW	10	27,043,643 (GRCm38)	frame shift	probably null
R4859:Lama2	UTSW	10	27,043,643 (GRCm38)	frame shift	probably null
R4897:Lama2	UTSW	10	27,043,643 (GRCm38)	frame shift	probably null
R4898:Lama2	UTSW	10	27,043,643 (GRCm38)	frame shift	probably null
R4899:Lama2	UTSW	10	27,043,643 (GRCm38)	frame shift	probably null
R4907:Lama2	UTSW	10	27,164,946 (GRCm38)	missense	probably benign	0.11
R4911:Lama2	UTSW	10	27,138,927 (GRCm38)	missense	probably damaging	1.00
R4924:Lama2	UTSW	10	27,369,141 (GRCm38)	missense	probably damaging	0.98
R5023:Lama2	UTSW	10	27,190,504 (GRCm38)	missense	probably damaging	0.97
R5057:Lama2	UTSW	10	27,164,986 (GRCm38)	missense	probably damaging	1.00
R5070:Lama2	UTSW	10	27,350,251 (GRCm38)	critical splice donor site	probably null
R5116:Lama2	UTSW	10	27,118,560 (GRCm38)	missense	probably benign	0.08
R5177:Lama2	UTSW	10	27,190,703 (GRCm38)	missense	possibly damaging	0.94
R5198:Lama2	UTSW	10	27,347,003 (GRCm38)	missense	probably damaging	0.96
R5289:Lama2	UTSW	10	27,212,073 (GRCm38)	nonsense	probably null
R5327:Lama2	UTSW	10	27,138,946 (GRCm38)	missense	probably benign
R5424:Lama2	UTSW	10	26,984,396 (GRCm38)	missense	probably damaging	1.00
R5469:Lama2	UTSW	10	27,041,189 (GRCm38)	missense	possibly damaging	0.92
R5620:Lama2	UTSW	10	26,990,880 (GRCm38)	missense	probably damaging	0.99
R5667:Lama2	UTSW	10	27,190,544 (GRCm38)	missense	probably damaging	1.00
R5671:Lama2	UTSW	10	27,190,544 (GRCm38)	missense	probably damaging	1.00
R5815:Lama2	UTSW	10	26,986,851 (GRCm38)	missense	probably damaging	1.00
R5917:Lama2	UTSW	10	27,190,697 (GRCm38)	missense	probably damaging	1.00
R5935:Lama2	UTSW	10	27,015,498 (GRCm38)	missense	probably benign
R5976:Lama2	UTSW	10	27,190,676 (GRCm38)	missense	probably benign	0.00
R5979:Lama2	UTSW	10	27,235,732 (GRCm38)	missense	probably damaging	0.99
R6004:Lama2	UTSW	10	27,235,785 (GRCm38)	missense	probably benign	0.01
R6180:Lama2	UTSW	10	26,981,499 (GRCm38)	missense	probably benign	0.03
R6198:Lama2	UTSW	10	27,188,022 (GRCm38)	missense	probably damaging	1.00
R6257:Lama2	UTSW	10	26,986,899 (GRCm38)	missense	possibly damaging	0.85
R6271:Lama2	UTSW	10	27,023,329 (GRCm38)	missense	possibly damaging	0.67
R6322:Lama2	UTSW	10	27,190,547 (GRCm38)	missense	probably damaging	0.96
R6354:Lama2	UTSW	10	27,212,068 (GRCm38)	missense	probably damaging	1.00
R6431:Lama2	UTSW	10	27,053,031 (GRCm38)	missense	possibly damaging	0.50
R6499:Lama2	UTSW	10	27,031,158 (GRCm38)	missense	probably damaging	1.00
R6535:Lama2	UTSW	10	27,104,131 (GRCm38)	missense	probably damaging	1.00
R6545:Lama2	UTSW	10	27,176,797 (GRCm38)	missense	probably benign
R6636:Lama2	UTSW	10	27,124,568 (GRCm38)	missense	probably benign	0.13
R6891:Lama2	UTSW	10	27,328,082 (GRCm38)	nonsense	probably null
R6891:Lama2	UTSW	10	27,328,072 (GRCm38)	nonsense	probably null
R6902:Lama2	UTSW	10	26,981,629 (GRCm38)	missense	probably damaging	1.00
R6908:Lama2	UTSW	10	27,031,196 (GRCm38)	splice site	probably null
R7168:Lama2	UTSW	10	27,366,152 (GRCm38)	critical splice acceptor site	probably null
R7233:Lama2	UTSW	10	27,231,663 (GRCm38)	missense	probably damaging	1.00
R7272:Lama2	UTSW	10	27,124,556 (GRCm38)	missense	probably damaging	1.00
R7274:Lama2	UTSW	10	27,119,980 (GRCm38)	missense	probably damaging	0.99
R7419:Lama2	UTSW	10	27,266,634 (GRCm38)	missense	probably benign
R7423:Lama2	UTSW	10	27,212,226 (GRCm38)	missense	probably benign	0.00
R7554:Lama2	UTSW	10	27,155,496 (GRCm38)	missense	probably damaging	1.00
R7569:Lama2	UTSW	10	27,265,050 (GRCm38)	missense	probably damaging	1.00
R7574:Lama2	UTSW	10	27,006,730 (GRCm38)	missense	probably benign	0.03
R7584:Lama2	UTSW	10	27,104,261 (GRCm38)	missense	possibly damaging	0.78
R7586:Lama2	UTSW	10	27,101,393 (GRCm38)	missense	probably benign	0.00
R7603:Lama2	UTSW	10	27,266,680 (GRCm38)	missense	possibly damaging	0.55
R7691:Lama2	UTSW	10	27,208,393 (GRCm38)	missense	possibly damaging	0.67
R7750:Lama2	UTSW	10	26,990,924 (GRCm38)	missense	probably damaging	0.97
R7841:Lama2	UTSW	10	27,155,533 (GRCm38)	missense	probably benign	0.00
R7864:Lama2	UTSW	10	27,056,615 (GRCm38)	missense	probably benign	0.08
R7960:Lama2	UTSW	10	26,993,098 (GRCm38)	missense	probably benign	0.04
R7964:Lama2	UTSW	10	27,223,981 (GRCm38)	critical splice donor site	probably null
R7980:Lama2	UTSW	10	27,363,613 (GRCm38)	missense	probably damaging	0.98
R8013:Lama2	UTSW	10	27,344,498 (GRCm38)	missense	probably benign	0.00
R8028:Lama2	UTSW	10	27,328,149 (GRCm38)	missense	probably benign	0.13
R8097:Lama2	UTSW	10	27,190,664 (GRCm38)	nonsense	probably null
R8100:Lama2	UTSW	10	27,041,117 (GRCm38)	missense	probably benign	0.03
R8110:Lama2	UTSW	10	26,990,870 (GRCm38)	missense	probably damaging	1.00
R8122:Lama2	UTSW	10	27,054,596 (GRCm38)	missense	possibly damaging	0.87
R8264:Lama2	UTSW	10	27,467,222 (GRCm38)	missense	probably benign	0.07
R8315:Lama2	UTSW	10	27,422,659 (GRCm38)	missense	probably damaging	1.00
R8318:Lama2	UTSW	10	26,984,338 (GRCm38)	missense	probably damaging	1.00
R8419:Lama2	UTSW	10	27,422,563 (GRCm38)	missense	probably benign	0.26
R8475:Lama2	UTSW	10	27,101,373 (GRCm38)	missense	possibly damaging	0.69
R8735:Lama2	UTSW	10	27,190,534 (GRCm38)	missense	probably damaging	1.00
R8754:Lama2	UTSW	10	27,001,151 (GRCm38)	missense	possibly damaging	0.83
R8817:Lama2	UTSW	10	27,187,873 (GRCm38)	missense	probably damaging	1.00
R8851:Lama2	UTSW	10	27,366,123 (GRCm38)	missense	possibly damaging	0.94
R8859:Lama2	UTSW	10	27,459,388 (GRCm38)	missense	possibly damaging	0.58
R8886:Lama2	UTSW	10	27,369,161 (GRCm38)	splice site	probably benign
R8937:Lama2	UTSW	10	26,986,820 (GRCm38)	missense	probably damaging	1.00
R8993:Lama2	UTSW	10	27,422,714 (GRCm38)	missense	possibly damaging	0.91
R9025:Lama2	UTSW	10	26,984,371 (GRCm38)	missense	probably benign	0.00
R9027:Lama2	UTSW	10	27,204,885 (GRCm38)	missense	probably damaging	1.00
R9047:Lama2	UTSW	10	27,006,701 (GRCm38)	missense	possibly damaging	0.50
R9075:Lama2	UTSW	10	26,981,592 (GRCm38)	missense	probably damaging	1.00
R9135:Lama2	UTSW	10	27,422,519 (GRCm38)	missense	probably damaging	1.00
R9165:Lama2	UTSW	10	27,053,026 (GRCm38)	critical splice donor site	probably null
R9192:Lama2	UTSW	10	27,328,185 (GRCm38)	missense	possibly damaging	0.95
R9254:Lama2	UTSW	10	27,422,689 (GRCm38)	missense	probably damaging	0.96
R9326:Lama2	UTSW	10	27,030,197 (GRCm38)	missense	probably benign	0.04
R9356:Lama2	UTSW	10	27,212,190 (GRCm38)	missense	probably damaging	0.99
R9358:Lama2	UTSW	10	27,616,765 (GRCm38)	missense	unknown
R9358:Lama2	UTSW	10	27,188,382 (GRCm38)	missense	possibly damaging	0.95
R9376:Lama2	UTSW	10	27,118,624 (GRCm38)	missense	probably benign	0.11
R9381:Lama2	UTSW	10	27,188,027 (GRCm38)	nonsense	probably null
R9397:Lama2	UTSW	10	27,105,121 (GRCm38)	missense	probably benign	0.01
R9460:Lama2	UTSW	10	27,422,479 (GRCm38)	missense	probably damaging	1.00
R9478:Lama2	UTSW	10	27,015,482 (GRCm38)	missense	probably damaging	0.98
R9503:Lama2	UTSW	10	26,989,444 (GRCm38)	missense	possibly damaging	0.57
R9514:Lama2	UTSW	10	27,224,019 (GRCm38)	missense	probably benign	0.00
R9515:Lama2	UTSW	10	27,001,174 (GRCm38)	missense	probably benign	0.23
R9516:Lama2	UTSW	10	27,224,019 (GRCm38)	missense	probably benign	0.00
R9533:Lama2	UTSW	10	26,986,875 (GRCm38)	missense	probably damaging	1.00
R9619:Lama2	UTSW	10	27,188,286 (GRCm38)	missense	probably damaging	1.00
R9721:Lama2	UTSW	10	27,467,342 (GRCm38)	missense	possibly damaging	0.58

Predicted Primers

PCR Primer
(F):5'- CACAGTTTTAGATAATGTGGATGCC -3'
(R):5'- AGTGCCATAGGACAGACGTG -3'

Sequencing Primer
(F):5'- GATGCCACAGGGACCTTCCTAC -3'
(R):5'- CGTGGCAGTAAAATTCTAGCTG -3'

Posted On

2015-02-05