Incidental Mutation 'R3147:Zfp85'
| ID |
264295 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp85
|
| Ensembl Gene |
ENSMUSG00000058331 |
| Gene Name |
zinc finger protein 85 |
| Synonyms |
Zfp85-rs1, KRAB19, Zfp71 |
| MMRRC Submission |
040599-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.079)
|
| Stock # |
R3147 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
13 |
| Chromosomal Location |
67895919-67903347 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to T
at 67900612 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Methionine
at position 10
(V10M)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000121202
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000091520]
[ENSMUST00000144183]
|
| AlphaFold |
Q7TMC9 |
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000049518
|
| Predicted Effect |
unknown
Transcript: ENSMUST00000091520
AA Change: M25I
|
| SMART Domains |
Protein: ENSMUSP00000089105
Gene: ENSMUSG00000058331
AA Change: M25I
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
5.78e-29 |
SMART |
|
ZnF_C2H2
|
81 |
103 |
1.95e-3 |
SMART |
|
ZnF_C2H2
|
109 |
131 |
1.22e-4 |
SMART |
|
ZnF_C2H2
|
137 |
159 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
165 |
187 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
193 |
215 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
221 |
243 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
249 |
271 |
1.1e-2 |
SMART |
|
ZnF_C2H2
|
277 |
299 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
305 |
327 |
2.49e-1 |
SMART |
|
ZnF_C2H2
|
333 |
355 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
361 |
383 |
4.17e-3 |
SMART |
|
ZnF_C2H2
|
389 |
411 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
417 |
439 |
1.3e-4 |
SMART |
|
ZnF_C2H2
|
445 |
467 |
1.04e-3 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000143812
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000144183
AA Change: V10M
PolyPhen 2
Score 0.963 (Sensitivity: 0.78; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000121202
Gene: ENSMUSG00000058331
AA Change: V10M
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
45 |
105 |
5.78e-29 |
SMART |
|
ZnF_C2H2
|
121 |
143 |
1.95e-3 |
SMART |
|
ZnF_C2H2
|
149 |
171 |
1.22e-4 |
SMART |
|
ZnF_C2H2
|
177 |
199 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
205 |
227 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
233 |
255 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
261 |
283 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
289 |
311 |
1.1e-2 |
SMART |
|
ZnF_C2H2
|
317 |
339 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
345 |
367 |
2.49e-1 |
SMART |
|
ZnF_C2H2
|
373 |
395 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
401 |
423 |
4.17e-3 |
SMART |
|
ZnF_C2H2
|
429 |
451 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
457 |
479 |
1.3e-4 |
SMART |
|
ZnF_C2H2
|
485 |
507 |
1.04e-3 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000181158
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.6%
- 10x: 97.2%
- 20x: 94.7%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 35 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4933436I01Rik |
T |
C |
X: 66,964,984 (GRCm39) |
D12G |
probably benign |
Het |
| Alg2 |
A |
T |
4: 47,472,259 (GRCm39) |
V183D |
probably damaging |
Het |
| Amy1 |
T |
C |
3: 113,363,697 (GRCm39) |
|
probably benign |
Het |
| Asb15 |
G |
T |
6: 24,566,258 (GRCm39) |
A404S |
probably damaging |
Het |
| Atf2 |
T |
C |
2: 73,681,283 (GRCm39) |
|
probably null |
Het |
| Atosa |
A |
G |
9: 74,916,120 (GRCm39) |
I240V |
probably benign |
Het |
| Baalc |
A |
T |
15: 38,812,568 (GRCm39) |
E106V |
possibly damaging |
Het |
| Catsperd |
G |
T |
17: 56,971,039 (GRCm39) |
C701F |
possibly damaging |
Het |
| Cc2d2a |
T |
A |
5: 43,866,497 (GRCm39) |
I769N |
probably damaging |
Het |
| Ccdc158 |
T |
C |
5: 92,805,822 (GRCm39) |
N311S |
probably damaging |
Het |
| Dbx1 |
C |
A |
7: 49,286,297 (GRCm39) |
R56L |
probably damaging |
Het |
| Eif4enif1 |
T |
A |
11: 3,194,003 (GRCm39) |
|
probably null |
Het |
| Elmod3 |
T |
G |
6: 72,563,485 (GRCm39) |
T48P |
probably benign |
Het |
| Erbb2 |
T |
C |
11: 98,324,865 (GRCm39) |
S820P |
probably damaging |
Het |
| Gimap8 |
T |
C |
6: 48,627,440 (GRCm39) |
V138A |
probably damaging |
Het |
| H1f5 |
T |
C |
13: 21,964,285 (GRCm39) |
|
probably benign |
Het |
| Il6ra |
G |
T |
3: 89,793,235 (GRCm39) |
P305Q |
probably benign |
Het |
| Kcng3 |
A |
G |
17: 83,895,749 (GRCm39) |
V239A |
possibly damaging |
Het |
| Kcnrg |
T |
C |
14: 61,845,140 (GRCm39) |
F60S |
probably damaging |
Het |
| Klk14 |
G |
A |
7: 43,341,501 (GRCm39) |
C51Y |
probably damaging |
Het |
| Lama1 |
A |
G |
17: 68,044,653 (GRCm39) |
D184G |
probably damaging |
Het |
| Lhcgr |
A |
T |
17: 89,065,771 (GRCm39) |
L206Q |
probably damaging |
Het |
| Lhx3 |
T |
C |
2: 26,091,277 (GRCm39) |
D344G |
probably benign |
Het |
| Marf1 |
A |
G |
16: 13,943,843 (GRCm39) |
V1380A |
possibly damaging |
Het |
| Mtcl2 |
T |
G |
2: 156,862,284 (GRCm39) |
K1548N |
possibly damaging |
Het |
| Mtfr1 |
T |
C |
3: 19,271,374 (GRCm39) |
V182A |
probably benign |
Het |
| Or14a259 |
A |
G |
7: 86,013,092 (GRCm39) |
L151S |
probably benign |
Het |
| Or5p64 |
C |
T |
7: 107,854,883 (GRCm39) |
G154D |
possibly damaging |
Het |
| Rsf1 |
CG |
CGACGGAGGAG |
7: 97,229,115 (GRCm39) |
|
probably benign |
Het |
| Snx4 |
A |
C |
16: 33,108,094 (GRCm39) |
D296A |
probably benign |
Het |
| Sox7 |
A |
T |
14: 64,186,083 (GRCm39) |
Y373F |
probably damaging |
Het |
| Tuba1b |
T |
C |
15: 98,830,386 (GRCm39) |
T145A |
probably benign |
Het |
| Usp32 |
T |
A |
11: 84,919,913 (GRCm39) |
N718I |
probably damaging |
Het |
| Wapl |
G |
A |
14: 34,447,106 (GRCm39) |
V648M |
probably damaging |
Het |
| Zgrf1 |
A |
G |
3: 127,377,797 (GRCm39) |
N1014S |
possibly damaging |
Het |
|
| Other mutations in Zfp85 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
R0383:Zfp85
|
UTSW |
13 |
67,896,791 (GRCm39) |
missense |
probably benign |
0.05 |
|
R1187:Zfp85
|
UTSW |
13 |
67,897,835 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1510:Zfp85
|
UTSW |
13 |
67,903,084 (GRCm39) |
intron |
probably benign |
|
|
R1775:Zfp85
|
UTSW |
13 |
67,897,823 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1803:Zfp85
|
UTSW |
13 |
67,899,747 (GRCm39) |
missense |
probably benign |
0.15 |
|
R2108:Zfp85
|
UTSW |
13 |
67,897,003 (GRCm39) |
missense |
probably benign |
0.08 |
|
R2412:Zfp85
|
UTSW |
13 |
67,897,765 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4811:Zfp85
|
UTSW |
13 |
67,897,745 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5308:Zfp85
|
UTSW |
13 |
67,896,974 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5334:Zfp85
|
UTSW |
13 |
67,899,803 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5373:Zfp85
|
UTSW |
13 |
67,897,577 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7045:Zfp85
|
UTSW |
13 |
67,897,712 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7391:Zfp85
|
UTSW |
13 |
67,897,410 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7438:Zfp85
|
UTSW |
13 |
67,897,064 (GRCm39) |
missense |
probably benign |
0.09 |
|
R7544:Zfp85
|
UTSW |
13 |
67,897,184 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8046:Zfp85
|
UTSW |
13 |
67,897,098 (GRCm39) |
nonsense |
probably null |
|
|
R8112:Zfp85
|
UTSW |
13 |
67,896,893 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R9533:Zfp85
|
UTSW |
13 |
67,897,722 (GRCm39) |
missense |
probably benign |
0.08 |
|
R9615:Zfp85
|
UTSW |
13 |
67,897,326 (GRCm39) |
missense |
probably damaging |
0.98 |
|
| Predicted Primers |
PCR Primer
(F):5'- TTCAAAGACAGGCAAGCAGTC -3'
(R):5'- ACTTATGTGCAGCGTAGTCC -3'
Sequencing Primer
(F):5'- AGCAGTCCCATGCTGACAGTG -3'
(R):5'- TAACACAGGTGTCTCCAATGGGC -3'
|
| Posted On |
2015-02-05 |
Incidental Mutation