Incidental Mutation 'R3149:Mettl25'
| ID |
264360 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mettl25
|
| Ensembl Gene |
ENSMUSG00000036009 |
| Gene Name |
methyltransferase like 25 |
| Synonyms |
BC067068 |
| MMRRC Submission |
040601-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.114)
|
| Stock # |
R3149 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
10 |
| Chromosomal Location |
105599050-105677241 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 105662214 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Aspartic acid to Glycine
at position 252
(D252G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000038665
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000046638]
[ENSMUST00000176040]
[ENSMUST00000176924]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000046638
AA Change: D252G
PolyPhen 2
Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
|
| SMART Domains |
Protein: ENSMUSP00000038665
Gene: ENSMUSG00000036009
AA Change: D252G
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Methyltransf_32
|
149 |
413 |
1.9e-30 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176040
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176924
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.7%
- 10x: 97.5%
- 20x: 95.7%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 31 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acsbg3 |
G |
A |
17: 57,183,348 (GRCm39) |
A30T |
probably benign |
Het |
| Atox1 |
A |
G |
11: 55,341,379 (GRCm39) |
L52P |
possibly damaging |
Het |
| Cel |
T |
C |
2: 28,446,143 (GRCm39) |
D576G |
probably benign |
Het |
| Csf2ra |
C |
A |
19: 61,215,758 (GRCm39) |
A16S |
possibly damaging |
Het |
| Cyp4f18 |
T |
C |
8: 72,747,044 (GRCm39) |
D317G |
possibly damaging |
Het |
| Dus1l |
T |
C |
11: 120,683,930 (GRCm39) |
T173A |
possibly damaging |
Het |
| Dzip1 |
T |
C |
14: 119,148,780 (GRCm39) |
T300A |
probably benign |
Het |
| Ggta1 |
A |
T |
2: 35,292,635 (GRCm39) |
I224N |
probably damaging |
Het |
| Gm5150 |
A |
G |
3: 16,060,479 (GRCm39) |
L3P |
probably damaging |
Het |
| Gm5592 |
A |
G |
7: 40,937,804 (GRCm39) |
E362G |
probably benign |
Het |
| Gm7137 |
T |
C |
10: 77,623,839 (GRCm39) |
|
probably benign |
Het |
| Gpatch2l |
A |
G |
12: 86,291,089 (GRCm39) |
T91A |
possibly damaging |
Het |
| Hoxa13 |
G |
T |
6: 52,237,284 (GRCm39) |
|
probably benign |
Het |
| Ift46 |
A |
G |
9: 44,695,045 (GRCm39) |
D65G |
probably damaging |
Het |
| Kirrel1 |
C |
T |
3: 86,996,458 (GRCm39) |
M380I |
probably null |
Het |
| Mapk11 |
T |
C |
15: 89,029,653 (GRCm39) |
|
probably null |
Het |
| Mrc2 |
G |
A |
11: 105,239,257 (GRCm39) |
|
probably null |
Het |
| Or10ab4 |
C |
T |
7: 107,654,989 (GRCm39) |
R267C |
probably benign |
Het |
| Pecam1 |
A |
G |
11: 106,575,107 (GRCm39) |
V601A |
possibly damaging |
Het |
| Prkx |
A |
T |
X: 76,814,881 (GRCm39) |
F260I |
probably damaging |
Het |
| Rassf9 |
A |
T |
10: 102,380,687 (GRCm39) |
D21V |
possibly damaging |
Het |
| Rmnd5a |
T |
A |
6: 71,406,085 (GRCm39) |
I68L |
probably benign |
Het |
| Rock2 |
T |
C |
12: 17,015,092 (GRCm39) |
S762P |
probably damaging |
Het |
| Septin4 |
T |
C |
11: 87,458,070 (GRCm39) |
V148A |
possibly damaging |
Het |
| Srgap2 |
T |
C |
1: 131,220,327 (GRCm39) |
T216A |
probably benign |
Het |
| Tasor2 |
G |
A |
13: 3,624,359 (GRCm39) |
P1182S |
probably damaging |
Het |
| Vmn1r86 |
T |
A |
7: 12,836,358 (GRCm39) |
K123* |
probably null |
Het |
| Vmn2r68 |
A |
C |
7: 84,886,875 (GRCm39) |
V13G |
probably benign |
Het |
| Vps13d |
G |
C |
4: 144,853,147 (GRCm39) |
N2322K |
possibly damaging |
Het |
| Xpo5 |
A |
G |
17: 46,553,173 (GRCm39) |
|
probably null |
Het |
| Zswim9 |
T |
C |
7: 13,011,196 (GRCm39) |
T51A |
possibly damaging |
Het |
|
| Other mutations in Mettl25 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00310:Mettl25
|
APN |
10 |
105,662,295 (GRCm39) |
missense |
probably benign |
|
|
IGL00698:Mettl25
|
APN |
10 |
105,629,201 (GRCm39) |
missense |
probably null |
0.31 |
|
IGL00766:Mettl25
|
APN |
10 |
105,615,443 (GRCm39) |
splice site |
probably benign |
|
|
IGL01360:Mettl25
|
APN |
10 |
105,659,058 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01954:Mettl25
|
APN |
10 |
105,659,068 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02088:Mettl25
|
APN |
10 |
105,659,111 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02623:Mettl25
|
APN |
10 |
105,662,185 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL03245:Mettl25
|
APN |
10 |
105,662,358 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL03134:Mettl25
|
UTSW |
10 |
105,661,888 (GRCm39) |
nonsense |
probably null |
|
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1297:Mettl25
|
UTSW |
10 |
105,659,126 (GRCm39) |
missense |
probably benign |
0.11 |
|
R1482:Mettl25
|
UTSW |
10 |
105,662,451 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1526:Mettl25
|
UTSW |
10 |
105,668,844 (GRCm39) |
missense |
possibly damaging |
0.56 |
|
R1542:Mettl25
|
UTSW |
10 |
105,661,981 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1589:Mettl25
|
UTSW |
10 |
105,615,493 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1901:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R1902:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R2016:Mettl25
|
UTSW |
10 |
105,633,167 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2355:Mettl25
|
UTSW |
10 |
105,599,316 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2920:Mettl25
|
UTSW |
10 |
105,601,038 (GRCm39) |
splice site |
probably null |
|
|
R4547:Mettl25
|
UTSW |
10 |
105,661,878 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4646:Mettl25
|
UTSW |
10 |
105,662,416 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4652:Mettl25
|
UTSW |
10 |
105,615,509 (GRCm39) |
nonsense |
probably null |
|
|
R5020:Mettl25
|
UTSW |
10 |
105,662,068 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R5435:Mettl25
|
UTSW |
10 |
105,615,447 (GRCm39) |
critical splice donor site |
probably null |
|
|
R6947:Mettl25
|
UTSW |
10 |
105,662,053 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7075:Mettl25
|
UTSW |
10 |
105,665,785 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R7729:Mettl25
|
UTSW |
10 |
105,601,871 (GRCm39) |
missense |
probably benign |
0.03 |
|
R8074:Mettl25
|
UTSW |
10 |
105,661,941 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8108:Mettl25
|
UTSW |
10 |
105,659,040 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R8415:Mettl25
|
UTSW |
10 |
105,662,002 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8937:Mettl25
|
UTSW |
10 |
105,601,122 (GRCm39) |
missense |
probably benign |
0.17 |
|
R8949:Mettl25
|
UTSW |
10 |
105,668,714 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9497:Mettl25
|
UTSW |
10 |
105,677,090 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9772:Mettl25
|
UTSW |
10 |
105,633,127 (GRCm39) |
missense |
probably benign |
|
|
RF009:Mettl25
|
UTSW |
10 |
105,669,100 (GRCm39) |
intron |
probably benign |
|
|
Z1176:Mettl25
|
UTSW |
10 |
105,661,959 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
| Predicted Primers |
PCR Primer
(F):5'- CTGCACCTGTGATGAAGTAGG -3'
(R):5'- TTTGAACGTTTATGGCATCGATTCC -3'
Sequencing Primer
(F):5'- TAGGTTCGATGGCATCAACAG -3'
(R):5'- GTTTATGGCATCGATTCCTCAAACAC -3'
|
| Posted On |
2015-02-05 |
Incidental Mutation