Mutagenetix > Incidental Mutation

Incidental Mutation 'R3080:Vmn2r1'

265369

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r1

Ensembl Gene

ENSMUSG00000027824

Gene Name

vomeronasal 2, receptor 1

Synonyms

V2r83, EG56544

MMRRC Submission

040570-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.111) question?

Stock #

R3080 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

63988968-64016905 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 63997205 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 287 (D287G)

Ref Sequence

ENSEMBL: ENSMUSP00000029406 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029406]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000029406
AA Change: D287G

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000029406
Gene: ENSMUSG00000027824
AA Change: D287G

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	86	504	6e-92	PFAM
Pfam:NCD3G	546	599	2.4e-17	PFAM
Pfam:7tm_3	632	866	4.1e-48	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Amer3	T	G	1: 34,627,503 (GRCm39)	L581V	probably benign	Het
Anks4b	A	T	7: 119,781,146 (GRCm39)	D59V	probably damaging	Het
Cdc42bpb	A	G	12: 111,262,252 (GRCm39)	I75T	probably damaging	Het
Ces1c	A	C	8: 93,846,975 (GRCm39)	L93R	probably damaging	Het
Ciao2b	G	C	8: 105,368,259 (GRCm39)	R22G	possibly damaging	Het
Clca4a	T	A	3: 144,669,551 (GRCm39)	K333N	probably damaging	Het
Copa	G	T	1: 171,940,716 (GRCm39)	D710Y	probably damaging	Het
Cyp3a25	T	A	5: 145,935,341 (GRCm39)	I92F	probably benign	Het
Dcx	T	A	X: 142,706,266 (GRCm39)	D175V	probably damaging	Het
Fam76b	G	A	9: 13,744,458 (GRCm39)	G185D	probably benign	Het
Fastkd5	T	C	2: 130,457,373 (GRCm39)	M406V	possibly damaging	Het
Fbn2	G	A	18: 58,282,122 (GRCm39)	S298L	probably damaging	Het
Gak	T	C	5: 108,761,468 (GRCm39)	K188E	possibly damaging	Het
Gemin2	G	A	12: 59,071,877 (GRCm39)	C230Y	probably damaging	Het
Gm5150	A	C	3: 16,045,085 (GRCm39)	S47A	possibly damaging	Het
H3c2	A	G	13: 23,936,481 (GRCm39)	H40R	possibly damaging	Het
Herc3	A	T	6: 58,833,631 (GRCm39)		probably null	Het
Ighv5-6	T	A	12: 113,589,237 (GRCm39)	D81V	probably damaging	Het
Igkv13-54-1	A	G	6: 69,594,454 (GRCm39)		probably null	Het
Itgad	T	C	7: 127,784,959 (GRCm39)	I288T	possibly damaging	Het
Krt1	C	T	15: 101,754,622 (GRCm39)	G543S	unknown	Het
Ltbp3	GTA	GTATA	19: 5,806,916 (GRCm39)		probably null	Het
Map4k2	G	A	19: 6,403,218 (GRCm39)	E774K	probably damaging	Het
Mbtps1	A	G	8: 120,257,944 (GRCm39)	V431A	probably benign	Het
Mbtps1	T	C	8: 120,265,602 (GRCm39)	D315G	probably damaging	Het
Nebl	A	G	2: 17,381,462 (GRCm39)	V738A	possibly damaging	Het
Npr3	G	C	15: 11,905,235 (GRCm39)	T164R	probably benign	Het
Or14j4	T	C	17: 37,921,169 (GRCm39)	T158A	probably benign	Het
Or6d14	T	G	6: 116,534,178 (GRCm39)	V264G	probably damaging	Het
Pcdhb3	T	G	18: 37,434,535 (GRCm39)	L167R	probably damaging	Het
Pcdhb8	A	T	18: 37,489,219 (GRCm39)	E299V	probably damaging	Het
Shcbp1l	A	G	1: 153,311,783 (GRCm39)	E312G	possibly damaging	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Slc1a2	T	G	2: 102,578,901 (GRCm39)	L272R	probably damaging	Het
Slc35a5	A	T	16: 44,964,758 (GRCm39)	S152T	probably benign	Het
Tas2r136	C	A	6: 132,754,972 (GRCm39)	V52L	probably damaging	Het
Tbr1	C	T	2: 61,637,635 (GRCm39)	Q65*	probably null	Het
Tro	G	A	X: 149,438,198 (GRCm39)	T153I	probably benign	Het
Vmn1r222	A	G	13: 23,416,631 (GRCm39)	M194T	possibly damaging	Het
Vmn2r91	T	G	17: 18,355,973 (GRCm39)		probably null	Het
Zfp35	T	A	18: 24,136,367 (GRCm39)	I237N	probably damaging	Het

Other mutations in Vmn2r1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00233:Vmn2r1	APN	3	64,012,389 (GRCm39)	nonsense	probably null
IGL00335:Vmn2r1	APN	3	64,012,809 (GRCm39)	missense	probably damaging	0.96
IGL01509:Vmn2r1	APN	3	64,010,466 (GRCm39)	missense	probably benign	0.03
IGL01641:Vmn2r1	APN	3	64,011,924 (GRCm39)	missense	probably benign	0.19
IGL01656:Vmn2r1	APN	3	63,989,274 (GRCm39)	missense	probably damaging	0.96
IGL01927:Vmn2r1	APN	3	63,989,105 (GRCm39)	missense	probably benign	0.01
IGL02093:Vmn2r1	APN	3	64,012,130 (GRCm39)	missense	probably benign
IGL02146:Vmn2r1	APN	3	64,012,104 (GRCm39)	missense	probably benign	0.39
IGL02186:Vmn2r1	APN	3	63,989,138 (GRCm39)	missense	probably benign	0.01
IGL02320:Vmn2r1	APN	3	63,989,180 (GRCm39)	missense	possibly damaging	0.61
IGL02423:Vmn2r1	APN	3	63,997,665 (GRCm39)	missense	probably benign	0.00
IGL02709:Vmn2r1	APN	3	64,012,355 (GRCm39)	missense	probably benign	0.24
R0034:Vmn2r1	UTSW	3	63,997,435 (GRCm39)	missense	probably damaging	1.00
R0064:Vmn2r1	UTSW	3	64,012,209 (GRCm39)	missense	possibly damaging	0.76
R0152:Vmn2r1	UTSW	3	63,989,240 (GRCm39)	missense	possibly damaging	0.92
R0305:Vmn2r1	UTSW	3	63,997,087 (GRCm39)	missense	probably damaging	1.00
R0314:Vmn2r1	UTSW	3	63,993,980 (GRCm39)	missense	probably damaging	1.00
R0317:Vmn2r1	UTSW	3	63,989,240 (GRCm39)	missense	possibly damaging	0.92
R0465:Vmn2r1	UTSW	3	63,989,180 (GRCm39)	missense	possibly damaging	0.61
R1144:Vmn2r1	UTSW	3	63,997,541 (GRCm39)	missense	probably damaging	1.00
R1163:Vmn2r1	UTSW	3	63,994,046 (GRCm39)	missense	probably benign	0.03
R1448:Vmn2r1	UTSW	3	64,008,734 (GRCm39)	missense	probably damaging	1.00
R1491:Vmn2r1	UTSW	3	63,997,034 (GRCm39)	missense	probably damaging	1.00
R1543:Vmn2r1	UTSW	3	63,996,994 (GRCm39)	missense	probably damaging	1.00
R1611:Vmn2r1	UTSW	3	64,011,958 (GRCm39)	nonsense	probably null
R1676:Vmn2r1	UTSW	3	63,997,603 (GRCm39)	nonsense	probably null
R1727:Vmn2r1	UTSW	3	63,989,163 (GRCm39)	missense	probably benign
R1851:Vmn2r1	UTSW	3	64,008,926 (GRCm39)	missense	probably benign	0.32
R3790:Vmn2r1	UTSW	3	63,994,185 (GRCm39)	critical splice donor site	probably null
R4111:Vmn2r1	UTSW	3	63,997,176 (GRCm39)	missense	probably benign	0.23
R4689:Vmn2r1	UTSW	3	64,012,074 (GRCm39)	missense	possibly damaging	0.94
R4747:Vmn2r1	UTSW	3	63,989,267 (GRCm39)	missense	probably benign	0.00
R4970:Vmn2r1	UTSW	3	63,997,544 (GRCm39)	missense	possibly damaging	0.81
R5033:Vmn2r1	UTSW	3	64,012,501 (GRCm39)	missense	probably damaging	1.00
R5086:Vmn2r1	UTSW	3	63,997,418 (GRCm39)	missense	probably benign	0.00
R5112:Vmn2r1	UTSW	3	63,997,544 (GRCm39)	missense	possibly damaging	0.81
R5385:Vmn2r1	UTSW	3	64,008,819 (GRCm39)	missense	possibly damaging	0.89
R5629:Vmn2r1	UTSW	3	64,012,538 (GRCm39)	missense	possibly damaging	0.87
R5762:Vmn2r1	UTSW	3	63,997,474 (GRCm39)	missense	probably benign	0.24
R5867:Vmn2r1	UTSW	3	64,011,990 (GRCm39)	missense	probably benign
R5893:Vmn2r1	UTSW	3	63,993,974 (GRCm39)	missense	probably damaging	1.00
R6037:Vmn2r1	UTSW	3	63,989,150 (GRCm39)	missense	probably benign	0.00
R6037:Vmn2r1	UTSW	3	63,989,150 (GRCm39)	missense	probably benign	0.00
R6290:Vmn2r1	UTSW	3	64,012,873 (GRCm39)	missense	probably benign	0.02
R6443:Vmn2r1	UTSW	3	64,012,374 (GRCm39)	missense	possibly damaging	0.78
R6464:Vmn2r1	UTSW	3	64,008,766 (GRCm39)	missense	probably benign
R6826:Vmn2r1	UTSW	3	64,012,567 (GRCm39)	nonsense	probably null
R6874:Vmn2r1	UTSW	3	64,012,376 (GRCm39)	missense	probably damaging	1.00
R6882:Vmn2r1	UTSW	3	63,997,529 (GRCm39)	missense	possibly damaging	0.64
R6983:Vmn2r1	UTSW	3	63,989,118 (GRCm39)	missense	probably benign
R7010:Vmn2r1	UTSW	3	64,012,146 (GRCm39)	missense	probably benign	0.19
R7144:Vmn2r1	UTSW	3	63,997,362 (GRCm39)	missense	probably damaging	1.00
R7341:Vmn2r1	UTSW	3	64,012,877 (GRCm39)	makesense	probably null
R7510:Vmn2r1	UTSW	3	63,993,922 (GRCm39)	missense	probably damaging	1.00
R7557:Vmn2r1	UTSW	3	63,997,475 (GRCm39)	missense	probably damaging	0.99
R7895:Vmn2r1	UTSW	3	63,997,130 (GRCm39)	missense	possibly damaging	0.90
R8108:Vmn2r1	UTSW	3	64,010,471 (GRCm39)	missense	probably damaging	0.98
R8209:Vmn2r1	UTSW	3	63,997,199 (GRCm39)	missense	possibly damaging	0.64
R8365:Vmn2r1	UTSW	3	63,994,034 (GRCm39)	missense	possibly damaging	0.80
R8514:Vmn2r1	UTSW	3	63,993,942 (GRCm39)	missense	probably benign	0.11
R8554:Vmn2r1	UTSW	3	63,997,334 (GRCm39)	missense	probably damaging	0.98
R8980:Vmn2r1	UTSW	3	64,010,501 (GRCm39)	missense
R9140:Vmn2r1	UTSW	3	63,997,465 (GRCm39)	missense	probably benign	0.05
R9239:Vmn2r1	UTSW	3	64,011,959 (GRCm39)	missense	probably damaging	0.99
R9441:Vmn2r1	UTSW	3	64,012,674 (GRCm39)	missense	probably damaging	1.00
R9549:Vmn2r1	UTSW	3	63,997,493 (GRCm39)	missense	probably benign	0.17
R9771:Vmn2r1	UTSW	3	63,997,559 (GRCm39)	missense	possibly damaging	0.79
X0065:Vmn2r1	UTSW	3	63,997,678 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AAACACTTCGGTTGGGTCTGG -3'
(R):5'- GACATCATTTGGATCCTTGTTAGGG -3'

Sequencing Primer
(F):5'- CTATTGCAGCTGATGATGATTATGG -3'
(R):5'- CCTTGTTAGGGTGTACATCATAAAG -3'

Posted On

2015-02-05