Mutagenetix > Incidental Mutation

Incidental Mutation 'R3080:Zfp35'

265400

Institutional Source

Beutler Lab

Gene Symbol

Zfp35

Ensembl Gene

ENSMUSG00000063281

Gene Name

zinc finger protein 35

Synonyms

Zfp-35

MMRRC Submission

040570-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.335) question?

Stock #

R3080 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

24122689-24138433 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 24136367 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 237 (I237N)

Ref Sequence

ENSEMBL: ENSMUSP00000074475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074941]

AlphaFold

P15620

Predicted Effect

probably damaging
Transcript: ENSMUST00000074941
AA Change: I237N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000074475
Gene: ENSMUSG00000063281
AA Change: I237N

Domain	Start	End	E-Value	Type
ZnF_C2H2	78	100	1.28e-3	SMART
ZnF_C2H2	104	126	8.47e-4	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	1.84e-4	SMART
ZnF_C2H2	188	210	7.37e-4	SMART
ZnF_C2H2	216	238	4.72e-2	SMART
ZnF_C2H2	244	266	2.4e-3	SMART
ZnF_C2H2	272	294	1.04e-3	SMART
ZnF_C2H2	300	322	3.69e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	1.5e-4	SMART
ZnF_C2H2	384	406	1.6e-4	SMART
ZnF_C2H2	412	434	3.89e-3	SMART
ZnF_C2H2	440	462	1.72e-4	SMART
ZnF_C2H2	468	490	2.71e-2	SMART
ZnF_C2H2	496	518	1.6e-4	SMART
ZnF_C2H2	524	545	2.17e1	SMART
ZnF_C2H2	551	573	1.6e-4	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out mutation exhibit increased airway responsiveness to ovalbumin and methacholine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Amer3	T	G	1: 34,627,503 (GRCm39)	L581V	probably benign	Het
Anks4b	A	T	7: 119,781,146 (GRCm39)	D59V	probably damaging	Het
Cdc42bpb	A	G	12: 111,262,252 (GRCm39)	I75T	probably damaging	Het
Ces1c	A	C	8: 93,846,975 (GRCm39)	L93R	probably damaging	Het
Ciao2b	G	C	8: 105,368,259 (GRCm39)	R22G	possibly damaging	Het
Clca4a	T	A	3: 144,669,551 (GRCm39)	K333N	probably damaging	Het
Copa	G	T	1: 171,940,716 (GRCm39)	D710Y	probably damaging	Het
Cyp3a25	T	A	5: 145,935,341 (GRCm39)	I92F	probably benign	Het
Dcx	T	A	X: 142,706,266 (GRCm39)	D175V	probably damaging	Het
Fam76b	G	A	9: 13,744,458 (GRCm39)	G185D	probably benign	Het
Fastkd5	T	C	2: 130,457,373 (GRCm39)	M406V	possibly damaging	Het
Fbn2	G	A	18: 58,282,122 (GRCm39)	S298L	probably damaging	Het
Gak	T	C	5: 108,761,468 (GRCm39)	K188E	possibly damaging	Het
Gemin2	G	A	12: 59,071,877 (GRCm39)	C230Y	probably damaging	Het
Gm5150	A	C	3: 16,045,085 (GRCm39)	S47A	possibly damaging	Het
H3c2	A	G	13: 23,936,481 (GRCm39)	H40R	possibly damaging	Het
Herc3	A	T	6: 58,833,631 (GRCm39)		probably null	Het
Ighv5-6	T	A	12: 113,589,237 (GRCm39)	D81V	probably damaging	Het
Igkv13-54-1	A	G	6: 69,594,454 (GRCm39)		probably null	Het
Itgad	T	C	7: 127,784,959 (GRCm39)	I288T	possibly damaging	Het
Krt1	C	T	15: 101,754,622 (GRCm39)	G543S	unknown	Het
Ltbp3	GTA	GTATA	19: 5,806,916 (GRCm39)		probably null	Het
Map4k2	G	A	19: 6,403,218 (GRCm39)	E774K	probably damaging	Het
Mbtps1	A	G	8: 120,257,944 (GRCm39)	V431A	probably benign	Het
Mbtps1	T	C	8: 120,265,602 (GRCm39)	D315G	probably damaging	Het
Nebl	A	G	2: 17,381,462 (GRCm39)	V738A	possibly damaging	Het
Npr3	G	C	15: 11,905,235 (GRCm39)	T164R	probably benign	Het
Or14j4	T	C	17: 37,921,169 (GRCm39)	T158A	probably benign	Het
Or6d14	T	G	6: 116,534,178 (GRCm39)	V264G	probably damaging	Het
Pcdhb3	T	G	18: 37,434,535 (GRCm39)	L167R	probably damaging	Het
Pcdhb8	A	T	18: 37,489,219 (GRCm39)	E299V	probably damaging	Het
Shcbp1l	A	G	1: 153,311,783 (GRCm39)	E312G	possibly damaging	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Slc1a2	T	G	2: 102,578,901 (GRCm39)	L272R	probably damaging	Het
Slc35a5	A	T	16: 44,964,758 (GRCm39)	S152T	probably benign	Het
Tas2r136	C	A	6: 132,754,972 (GRCm39)	V52L	probably damaging	Het
Tbr1	C	T	2: 61,637,635 (GRCm39)	Q65*	probably null	Het
Tro	G	A	X: 149,438,198 (GRCm39)	T153I	probably benign	Het
Vmn1r222	A	G	13: 23,416,631 (GRCm39)	M194T	possibly damaging	Het
Vmn2r1	A	G	3: 63,997,205 (GRCm39)	D287G	probably damaging	Het
Vmn2r91	T	G	17: 18,355,973 (GRCm39)		probably null	Het

Other mutations in Zfp35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0012:Zfp35	UTSW	18	24,136,001 (GRCm39)	missense	probably benign	0.01
R1453:Zfp35	UTSW	18	24,136,557 (GRCm39)	nonsense	probably null
R1807:Zfp35	UTSW	18	24,136,986 (GRCm39)	missense	probably benign	0.28
R2317:Zfp35	UTSW	18	24,136,555 (GRCm39)	missense	probably damaging	1.00
R2863:Zfp35	UTSW	18	24,137,352 (GRCm39)	missense	probably damaging	0.99
R3756:Zfp35	UTSW	18	24,135,982 (GRCm39)	missense	possibly damaging	0.88
R4114:Zfp35	UTSW	18	24,135,766 (GRCm39)	missense	probably benign	0.08
R4222:Zfp35	UTSW	18	24,136,246 (GRCm39)	missense	possibly damaging	0.91
R4780:Zfp35	UTSW	18	24,136,326 (GRCm39)	missense	probably damaging	1.00
R5137:Zfp35	UTSW	18	24,137,194 (GRCm39)	nonsense	probably null
R5261:Zfp35	UTSW	18	24,136,778 (GRCm39)	missense	probably damaging	0.98
R5375:Zfp35	UTSW	18	24,135,973 (GRCm39)	missense	possibly damaging	0.83
R6001:Zfp35	UTSW	18	24,135,816 (GRCm39)	missense	probably benign
R6190:Zfp35	UTSW	18	24,137,118 (GRCm39)	missense	probably benign	0.00
R6774:Zfp35	UTSW	18	24,136,015 (GRCm39)	missense	possibly damaging	0.51
R6850:Zfp35	UTSW	18	24,135,839 (GRCm39)	missense	possibly damaging	0.76
R6979:Zfp35	UTSW	18	24,136,927 (GRCm39)	missense	probably benign	0.32
R7029:Zfp35	UTSW	18	24,136,583 (GRCm39)	missense	probably damaging	0.98
R7079:Zfp35	UTSW	18	24,136,357 (GRCm39)	missense	possibly damaging	0.89
R7858:Zfp35	UTSW	18	24,136,897 (GRCm39)	missense	probably damaging	1.00
R8257:Zfp35	UTSW	18	24,137,288 (GRCm39)	missense	possibly damaging	0.55
R9477:Zfp35	UTSW	18	24,136,188 (GRCm39)	missense	possibly damaging	0.86
R9642:Zfp35	UTSW	18	24,137,155 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCTGGTTCTGCATCAGAGAATT -3'
(R):5'- TGCAGAATAAGGACTGAGCTC -3'

Sequencing Primer
(F):5'- AAGTTTTAGTCGGAGCTCAGACC -3'
(R):5'- GACTGAGCTCTGGTTAAAAGCCTTC -3'

Posted On

2015-02-05