Mutagenetix > Incidental Mutation

Incidental Mutation 'R3432:Zfp872'

266238

Institutional Source

Beutler Lab

Gene Symbol

Zfp872

Ensembl Gene

ENSMUSG00000074472

Gene Name

zinc finger protein 872

Synonyms

9530015I07Rik

MMRRC Submission

040650-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R3432 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

22099462-22113419 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 22111750 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 410 (R410*)

Ref Sequence

ENSEMBL: ENSMUSP00000136074 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091508] [ENSMUST00000178901] [ENSMUST00000217301]

AlphaFold

Q3UVL3

Predicted Effect

probably null
Transcript: ENSMUST00000091508
AA Change: R409*

SMART Domains

Protein: ENSMUSP00000089089
Gene: ENSMUSG00000074472
AA Change: R409*

Domain	Start	End	E-Value	Type
KRAB	3	74	6.16e-15	SMART
ZnF_C2H2	99	121	1.64e-1	SMART
ZnF_C2H2	137	159	3.63e-3	SMART
ZnF_C2H2	165	187	5.14e-3	SMART
ZnF_C2H2	193	215	1.58e-3	SMART
ZnF_C2H2	221	243	2.75e-3	SMART
ZnF_C2H2	249	271	4.47e-3	SMART
ZnF_C2H2	277	299	2.57e-3	SMART
ZnF_C2H2	305	327	2.75e-3	SMART
ZnF_C2H2	333	355	3.58e-2	SMART
ZnF_C2H2	361	383	4.05e-1	SMART
ZnF_C2H2	389	411	2.43e-4	SMART
ZnF_C2H2	417	439	1.26e-2	SMART
ZnF_C2H2	445	463	1.27e2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000178901
AA Change: R410*

SMART Domains

Protein: ENSMUSP00000136074
Gene: ENSMUSG00000074472
AA Change: R410*

Domain	Start	End	E-Value	Type
KRAB	4	75	6.16e-15	SMART
ZnF_C2H2	100	122	1.64e-1	SMART
ZnF_C2H2	138	160	3.63e-3	SMART
ZnF_C2H2	166	188	5.14e-3	SMART
ZnF_C2H2	194	216	1.58e-3	SMART
ZnF_C2H2	222	244	2.75e-3	SMART
ZnF_C2H2	250	272	4.47e-3	SMART
ZnF_C2H2	278	300	2.57e-3	SMART
ZnF_C2H2	306	328	2.75e-3	SMART
ZnF_C2H2	334	356	3.58e-2	SMART
ZnF_C2H2	362	384	4.05e-1	SMART
ZnF_C2H2	390	412	2.43e-4	SMART
ZnF_C2H2	418	440	1.26e-2	SMART
ZnF_C2H2	446	464	1.27e2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000187399

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000217297

Predicted Effect

probably benign
Transcript: ENSMUST00000217301

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 93.7%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr3	G	A	1: 125,321,776 (GRCm39)	P405S	probably damaging	Het
Adamts3	A	G	5: 89,855,312 (GRCm39)		probably benign	Het
Angptl2	T	C	2: 33,118,814 (GRCm39)	V196A	probably benign	Het
Aoc1	A	T	6: 48,882,778 (GRCm39)	H240L	probably damaging	Het
Arsj	A	T	3: 126,158,624 (GRCm39)	T68S	probably benign	Het
Atp8b3	T	C	10: 80,362,014 (GRCm39)	K708E	probably benign	Het
Bahd1	G	A	2: 118,753,004 (GRCm39)	R757H	probably damaging	Het
Ceacam5	T	A	7: 17,448,901 (GRCm39)	V89E	probably benign	Het
Cpt1b	T	C	15: 89,307,944 (GRCm39)	E205G	possibly damaging	Het
Dhrs7c	C	T	11: 67,700,699 (GRCm39)	T82I	probably benign	Het
Efs	C	T	14: 55,157,681 (GRCm39)	R117Q	probably damaging	Het
Evl	T	C	12: 108,614,567 (GRCm39)		probably benign	Het
Fam89b	A	T	19: 5,781,761 (GRCm39)		probably null	Het
Fbxo16	T	C	14: 65,531,233 (GRCm39)	F46L	probably damaging	Het
Glp1r	T	A	17: 31,143,531 (GRCm39)	L189H	probably damaging	Het
Gstp1	G	A	19: 4,086,695 (GRCm39)	T110I	possibly damaging	Het
Hbq1a	T	G	11: 32,250,715 (GRCm39)	S133A	probably benign	Het
Hhipl1	A	G	12: 108,277,948 (GRCm39)	E92G	probably damaging	Het
Il18r1	C	T	1: 40,526,249 (GRCm39)	T265M	probably damaging	Het
Lpp	T	C	16: 24,708,636 (GRCm39)	V447A	probably benign	Het
Mcm2	G	A	6: 88,869,990 (GRCm39)	R60C	probably damaging	Het
Mfsd13a	C	T	19: 46,360,431 (GRCm39)	R328C	probably damaging	Het
Mmel1	C	T	4: 154,969,955 (GRCm39)		probably benign	Het
Myo5c	A	G	9: 75,170,283 (GRCm39)	E471G	probably damaging	Het
Obscn	A	G	11: 58,922,003 (GRCm39)	L317P	probably damaging	Het
Or8b57	A	T	9: 40,003,845 (GRCm39)	M139K	probably damaging	Het
Psg18	A	G	7: 18,083,096 (GRCm39)	V232A	possibly damaging	Het
Ptprt	T	C	2: 161,769,449 (GRCm39)	E472G	probably damaging	Het
Rap2a	G	T	14: 120,741,170 (GRCm39)	A158S	possibly damaging	Het
Rbm15	A	T	3: 107,237,993 (GRCm39)	S802T	probably benign	Het
Sec14l5	A	G	16: 4,996,463 (GRCm39)	I470V	possibly damaging	Het
Serpinb1a	T	C	13: 33,026,842 (GRCm39)	T367A	possibly damaging	Het
Sirpb1a	A	G	3: 15,491,447 (GRCm39)	W7R	probably damaging	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Taf5	A	C	19: 47,064,272 (GRCm39)	K405T	probably damaging	Het
Tbc1d19	T	C	5: 54,005,548 (GRCm39)		probably benign	Het
Trim58	T	C	11: 58,537,787 (GRCm39)		probably benign	Het
Tssk4	A	G	14: 55,889,152 (GRCm39)	N226S	probably damaging	Het
Uggt1	C	A	1: 36,249,140 (GRCm39)	E267*	probably null	Het
Zdhhc23	T	A	16: 43,794,533 (GRCm39)		probably benign	Het
Zfp407	G	A	18: 84,226,871 (GRCm39)	A2246V	probably damaging	Het

Other mutations in Zfp872

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0211:Zfp872	UTSW	9	22,111,469 (GRCm39)	missense	probably damaging	0.98
R0211:Zfp872	UTSW	9	22,111,469 (GRCm39)	missense	probably damaging	0.98
R1162:Zfp872	UTSW	9	22,110,910 (GRCm39)	missense	possibly damaging	0.84
R1793:Zfp872	UTSW	9	22,111,349 (GRCm39)	missense	probably damaging	1.00
R4633:Zfp872	UTSW	9	22,108,490 (GRCm39)	critical splice donor site	probably null
R4647:Zfp872	UTSW	9	22,111,057 (GRCm39)	missense	possibly damaging	0.80
R4675:Zfp872	UTSW	9	22,108,701 (GRCm39)	missense	probably damaging	0.99
R6189:Zfp872	UTSW	9	22,108,427 (GRCm39)	missense	probably benign	0.01
R6240:Zfp872	UTSW	9	22,111,180 (GRCm39)	missense	probably damaging	1.00
R6852:Zfp872	UTSW	9	22,111,355 (GRCm39)	missense	probably damaging	1.00
R7001:Zfp872	UTSW	9	22,111,912 (GRCm39)	missense	probably damaging	1.00
R7036:Zfp872	UTSW	9	22,111,856 (GRCm39)	missense	probably benign	0.08
R7834:Zfp872	UTSW	9	22,111,406 (GRCm39)	missense	probably damaging	0.97
R8364:Zfp872	UTSW	9	22,111,540 (GRCm39)	missense	probably damaging	1.00
R8385:Zfp872	UTSW	9	22,111,407 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TTGTAAGGAGTGTGGGAAAGCC -3'
(R):5'- CACTGCTGACACTCATAGGGTT -3'

Sequencing Primer
(F):5'- CCAAGCTTTCGAAGGCATGTG -3'
(R):5'- GAGTCCTCTCATGAATTCGGACAG -3'

Posted On

2015-02-18