Mutagenetix > Incidental Mutation

Incidental Mutation 'R2870:Olfr801'

266532

Institutional Source

Beutler Lab

Gene Symbol

Olfr801

Ensembl Gene

ENSMUSG00000058513

Gene Name

olfactory receptor 801

Synonyms

GA_x6K02T2PULF-11349138-11348182, MOR110-10

MMRRC Submission

040458-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R2870 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

129666989-129673865 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 129669759 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 253 (C253*)

Ref Sequence

ENSEMBL: ENSMUSP00000149555 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072063] [ENSMUST00000203424] [ENSMUST00000213294] [ENSMUST00000216067]

AlphaFold

Q8VEU1

Predicted Effect

probably null
Transcript: ENSMUST00000072063
AA Change: C253*

SMART Domains

Protein: ENSMUSP00000071941
Gene: ENSMUSG00000058513
AA Change: C253*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	1.2e-51	PFAM
Pfam:7tm_1	39	288	8.8e-25	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203321

Predicted Effect

probably benign
Transcript: ENSMUST00000203424

SMART Domains

Protein: ENSMUSP00000145011
Gene: ENSMUSG00000058513

Domain	Start	End	E-Value	Type
Pfam:7tm_4	1	96	5.9e-18	PFAM
Pfam:7tm_1	2	96	1.3e-17	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213294
AA Change: C253*

Predicted Effect

probably null
Transcript: ENSMUST00000216067
AA Change: C253*

Meta Mutation Damage Score

0.9493

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930444G20Rik	A	T	10: 22,067,379	I234N	probably benign	Het
Actrt3	A	G	3: 30,599,698	V51A	probably damaging	Het
Adgb	C	T	10: 10,431,281		probably null	Het
AI481877	A	C	4: 59,093,850	L226R	probably damaging	Het
Als2	A	G	1: 59,211,137	S483P	probably damaging	Het
Ankrd10	C	T	8: 11,615,682	R306H	probably damaging	Het
Arhgef10	T	C	8: 14,975,093		probably null	Het
Arhgef10	A	G	8: 14,975,666	I459V	probably benign	Het
Armc2	C	T	10: 41,966,700		probably null	Het
Atp12a	A	G	14: 56,386,950	R952G	possibly damaging	Het
Atp6v1g1	A	G	4: 63,550,021	Y87C	probably benign	Het
C030034I22Rik	T	A	17: 69,418,111		noncoding transcript	Het
Cacna2d1	G	A	5: 16,312,568	C404Y	probably damaging	Het
Ccdc163	T	C	4: 116,741,861		silent	Het
Ccdc59	A	T	10: 105,841,527	K9M	possibly damaging	Het
Cd6	A	G	19: 10,794,626	I307T	possibly damaging	Het
Clasrp	C	A	7: 19,585,240		probably benign	Het
Csmd2	T	C	4: 128,557,718	F113S	unknown	Het
Csmd3	C	T	15: 47,857,924	G1437D	probably damaging	Het
Cyp4a14	C	A	4: 115,487,301	G456W	probably damaging	Het
Cyp4a30b	A	G	4: 115,458,362	H260R	possibly damaging	Het
Dcp1b	C	T	6: 119,214,774	S217L	probably benign	Het
Dhx57	A	T	17: 80,251,376	D1051E	probably benign	Het
Dmp1	A	G	5: 104,212,108	S217G	probably benign	Het
Eif4enif1	C	T	11: 3,242,586	P805S	probably damaging	Het
Eral1	A	G	11: 78,076,278	I164T	possibly damaging	Het
Esr1	G	A	10: 4,997,890	R481H	probably damaging	Het
Fam19a2	A	T	10: 123,704,365	H42L	possibly damaging	Het
Fan1	A	G	7: 64,363,190	I668T	probably benign	Het
Gbp11	C	T	5: 105,331,000	D191N	probably benign	Het
Gm21759	T	A	5: 8,180,863		probably benign	Het
Gm5454	C	A	13: 103,357,523		noncoding transcript	Het
Gm9874	A	T	17: 30,485,789		probably benign	Het
Gria2	G	A	3: 80,702,492	T670I	probably damaging	Het
Gria4	T	A	9: 4,503,614	N334I	probably damaging	Het
Grm5	T	C	7: 87,602,722	V60A	possibly damaging	Het
Ift172	C	T	5: 31,257,861	V1335I	probably benign	Het
Ino80d	C	T	1: 63,061,039		probably null	Het
Kif1c	A	G	11: 70,724,081	E567G	probably damaging	Het
Krt31	T	G	11: 100,047,873	N298T	possibly damaging	Het
Mapk7	C	A	11: 61,490,212		probably benign	Het
March8	C	T	6: 116,401,145		probably benign	Het
Matr3	T	A	18: 35,572,296	S91R	probably benign	Het
Mdm1	A	G	10: 118,150,942	T267A	probably benign	Het
Mlxip	A	G	5: 123,452,667	M878V	probably benign	Het
Mtm1	T	C	X: 71,296,362		probably benign	Homo
Mtor	T	A	4: 148,540,030	M2089K	probably benign	Het
Mylk2	A	G	2: 152,919,348	K457R	probably damaging	Het
Nell1	A	T	7: 50,249,657		probably benign	Het
Nomo1	C	A	7: 46,046,937	T293N	probably damaging	Het
Odf3l2	G	A	10: 79,645,653	T14I	probably benign	Het
Olfr1080	T	C	2: 86,553,584	D180G	possibly damaging	Het
Olfr1510	T	G	14: 52,410,861	T4P	probably benign	Het
Ostc	T	C	3: 130,703,508	N80S	probably damaging	Het
Otud4	T	A	8: 79,661,073	N300K	possibly damaging	Het
Otx1	A	G	11: 21,998,681		probably benign	Het
Palmd	T	C	3: 116,923,751	R366G	possibly damaging	Het
Pcdhb20	A	T	18: 37,505,780	Q453L	possibly damaging	Het
Pcdhga9	T	A	18: 37,737,471	Y118N	possibly damaging	Het
Pes1	C	A	11: 3,976,834	T372K	probably benign	Het
Plcl1	A	T	1: 55,697,150	D550V	probably benign	Het
Plekhg5	T	A	4: 152,107,503	C433S	probably benign	Het
Plin2	A	G	4: 86,668,678	M1T	probably null	Het
Pold1	C	T	7: 44,543,347		silent	Het
Ppp1r7	T	A	1: 93,357,863		probably null	Het
Psmb8	T	C	17: 34,200,170	I146T	probably damaging	Het
Pzp	A	T	6: 128,485,556		probably null	Het
Rel	T	C	11: 23,761,129	I13V	probably benign	Het
Reln	C	T	5: 22,049,791	V527I	possibly damaging	Het
Retnla	A	G	16: 48,843,612	R90G	probably benign	Het
Slc39a8	T	A	3: 135,886,793		probably null	Het
Slc5a8	A	G	10: 88,904,963	I247V	probably benign	Het
Son	A	G	16: 91,664,317		probably null	Het
Spcs2	T	C	7: 99,839,761	D240G	probably damaging	Het
St5	A	T	7: 109,557,430	Y38N	probably benign	Het
Stx3	A	T	19: 11,789,574	V91D	probably damaging	Het
Taf6l	A	G	19: 8,778,628		probably benign	Het
Tbc1d8	A	G	1: 39,405,317	F187S	probably damaging	Het
Thbs1	C	G	2: 118,119,378	N611K	probably damaging	Het
Tipin	A	C	9: 64,304,327	S232R	probably benign	Het
Tmem132b	A	G	5: 125,638,268	D347G	probably benign	Het
Tmem161a	C	T	8: 70,178,915		probably benign	Het
Vmn2r68	A	C	7: 85,233,626	M306R	probably benign	Het
Vwa7	G	A	17: 35,021,242	M395I	probably damaging	Het
Ybx3	G	A	6: 131,370,413	A253V	probably damaging	Het
Zfp53	A	T	17: 21,508,078	E124D	probably benign	Het
Zzz3	T	A	3: 152,446,844		silent	Het

Other mutations in Olfr801

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Olfr801	APN	10	129669887	missense	probably damaging	1.00
IGL02400:Olfr801	APN	10	129669883	missense	probably damaging	0.99
IGL02721:Olfr801	APN	10	129670123	missense	probably benign	0.08
IGL03107:Olfr801	APN	10	129669940	missense	probably benign	0.00
R0114:Olfr801	UTSW	10	129669598	missense	probably benign
R0140:Olfr801	UTSW	10	129669688	missense	probably damaging	1.00
R0652:Olfr801	UTSW	10	129670379	missense	probably benign	0.08
R1375:Olfr801	UTSW	10	129670372	missense	probably null	0.97
R1449:Olfr801	UTSW	10	129670369	missense	probably damaging	0.99
R2026:Olfr801	UTSW	10	129670465	missense	probably damaging	1.00
R2241:Olfr801	UTSW	10	129669895	missense	probably damaging	1.00
R2516:Olfr801	UTSW	10	129670286	missense	probably damaging	1.00
R2869:Olfr801	UTSW	10	129669759	nonsense	probably null
R2869:Olfr801	UTSW	10	129669759	nonsense	probably null
R2870:Olfr801	UTSW	10	129669759	nonsense	probably null
R2873:Olfr801	UTSW	10	129669759	nonsense	probably null
R6562:Olfr801	UTSW	10	129670139	missense	probably damaging	1.00
R8362:Olfr801	UTSW	10	129670231	missense	possibly damaging	0.88
R8503:Olfr801	UTSW	10	129669643	missense	probably damaging	0.99
R8867:Olfr801	UTSW	10	129669759	nonsense	probably null
R9251:Olfr801	UTSW	10	129670111	missense	possibly damaging	0.61
Z1177:Olfr801	UTSW	10	129669794	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- TTGTGAGAATACAGTCGGTGAAGC -3'
(R):5'- GACCACTTCCTGTGTGACAC -3'

Sequencing Primer
(F):5'- TAGCAAAGGTGCCACAGA -3'
(R):5'- GACACACATTTCATGGAATTGATGGC -3'

Posted On

2015-02-18