Mutagenetix > Incidental Mutation

Incidental Mutation 'R3419:Olfr146'

266972

Institutional Source

Beutler Lab

Gene Symbol

Olfr146

Ensembl Gene

ENSMUSG00000058820

Gene Name

olfactory receptor 146

Synonyms

GA_x6K02T2PVTD-32715386-32714466, MOR171-10, M15

MMRRC Submission

040637-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R3419 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

39018073-39023377 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 39019076 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Tyrosine at position 155 (C155Y)

Ref Sequence

ENSEMBL: ENSMUSP00000149294 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073671] [ENSMUST00000214369] [ENSMUST00000214410] [ENSMUST00000215383]

AlphaFold

Q60884

Predicted Effect

probably benign
Transcript: ENSMUST00000073671
AA Change: C155Y

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000073352
Gene: ENSMUSG00000058820
AA Change: C155Y

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	306	7.6e-54	PFAM
Pfam:7tm_1	41	290	8.1e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214369

Predicted Effect

probably benign
Transcript: ENSMUST00000214410
AA Change: C155Y

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

Predicted Effect

probably benign
Transcript: ENSMUST00000215383

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5530400C23Rik	A	G	6: 133,294,119	Q42R	probably benign	Het
Ankrd26	A	G	6: 118,535,107	L518P	probably damaging	Het
Armc9	T	C	1: 86,194,338	L395P	probably damaging	Het
Baz1a	T	C	12: 54,946,899	K181E	probably benign	Het
Bend3	T	C	10: 43,509,982	S124P	probably damaging	Het
Cdh5	C	T	8: 104,129,370	R312C	probably damaging	Het
Col6a3	T	C	1: 90,804,091	D873G	probably benign	Het
Dmbx1	G	C	4: 115,920,676	R64G	probably benign	Het
Dpy19l2	T	C	9: 24,581,205	E699G	probably damaging	Het
Eef2k	T	A	7: 120,885,870	M320K	probably damaging	Het
Exoc5	A	T	14: 49,023,278	N377K	probably damaging	Het
Fam20c	A	T	5: 138,757,868	N220Y	probably damaging	Het
Fchsd2	T	A	7: 101,278,660		probably null	Het
Flrt2	A	G	12: 95,780,604	Y572C	probably damaging	Het
Gm17093	A	G	14: 44,521,590	I190V	unknown	Het
Gsto1	T	C	19: 47,857,905	F64L	probably benign	Het
Gucy1a1	A	G	3: 82,106,133	S401P	probably damaging	Het
Hoxd10	A	G	2: 74,692,577	K200E	probably benign	Het
Kcnj13	T	C	1: 87,386,919	T194A	probably benign	Het
Klk14	G	A	7: 43,692,077	C51Y	probably damaging	Het
Lacc1	T	G	14: 77,034,881	E158D	probably benign	Het
Lyn	A	T	4: 3,746,833	I204F	probably damaging	Het
Mapk6	T	C	9: 75,397,757	E126G	probably damaging	Het
Mbd6	C	G	10: 127,286,503	R152P	probably null	Het
Olfr672	A	G	7: 104,996,520	I128T	probably damaging	Het
Olfr93	A	T	17: 37,151,351	V53E	probably damaging	Het
P2ry1	T	C	3: 61,003,712	F91L	probably damaging	Het
Paqr3	A	G	5: 97,099,700	L183P	probably damaging	Het
Pcdh15	C	A	10: 74,584,222	D1166E	probably benign	Het
Pik3r1	T	C	13: 101,692,215	D25G	probably benign	Het
Poc5	A	T	13: 96,404,417	T365S	possibly damaging	Het
Polr3a	A	G	14: 24,467,035	L716P	probably damaging	Het
Ppp1r3a	A	T	6: 14,719,414	D500E	probably benign	Het
Ptcd3	A	T	6: 71,883,486	I579K	possibly damaging	Het
Rnf168	A	G	16: 32,299,192	N524D	probably benign	Het
Scn7a	AT	ATT	2: 66,700,895		probably null	Het
Sel1l	A	G	12: 91,810,002	W689R	probably damaging	Het
Serpinb13	T	C	1: 106,998,927	S218P	probably damaging	Het
Serpini1	A	G	3: 75,640,282	Y367C	probably damaging	Het
Snrnp48	G	A	13: 38,221,359	D248N	possibly damaging	Het
St6galnac4	A	G	2: 32,595,731	T217A	probably damaging	Het
Tdrd1	T	A	19: 56,831,231	N54K	possibly damaging	Het
Tmem30c	A	G	16: 57,277,668	V130A	probably benign	Het
Trpa1	T	C	1: 14,874,381	I1046M	probably benign	Het
Tulp2	T	A	7: 45,518,752	M196K	possibly damaging	Het
Unc5b	G	A	10: 60,778,814	R235W	probably damaging	Het
Vmn1r175	A	T	7: 23,808,650	M184K	probably damaging	Het
Vmn2r2	A	T	3: 64,116,899	F754I	probably benign	Het
Vmn2r9	A	C	5: 108,846,433	M450R	probably damaging	Het
Wdr60	C	T	12: 116,224,977	V666I	probably benign	Het

Other mutations in Olfr146

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01533:Olfr146	APN	9	39018801	missense	probably damaging	0.98
IGL01655:Olfr146	APN	9	39018918	missense	probably benign	0.00
IGL01804:Olfr146	APN	9	39019401	missense	probably benign	0.13
IGL02098:Olfr146	APN	9	39018891	missense	probably damaging	1.00
IGL02719:Olfr146	APN	9	39019016	missense	probably benign	0.11
R0531:Olfr146	UTSW	9	39019176	missense	probably damaging	0.97
R1270:Olfr146	UTSW	9	39019247	missense	possibly damaging	0.90
R1511:Olfr146	UTSW	9	39019025	missense	probably benign	0.03
R1590:Olfr146	UTSW	9	39018957	missense	probably benign	0.09
R1649:Olfr146	UTSW	9	39019480	missense	probably benign	0.03
R4669:Olfr146	UTSW	9	39019379	missense	probably benign	0.10
R4788:Olfr146	UTSW	9	39018921	missense	probably benign	0.07
R5184:Olfr146	UTSW	9	39018702	missense	probably damaging	0.98
R5581:Olfr146	UTSW	9	39018702	missense	probably damaging	0.98
R6032:Olfr146	UTSW	9	39018965	missense	probably benign	0.00
R6032:Olfr146	UTSW	9	39018965	missense	probably benign	0.00
R6319:Olfr146	UTSW	9	39019514	missense	probably damaging	1.00
R6626:Olfr146	UTSW	9	39019106	missense	possibly damaging	0.63
R6693:Olfr146	UTSW	9	39018801	missense	probably damaging	0.98
R7165:Olfr146	UTSW	9	39023270	start gained	probably benign
R7947:Olfr146	UTSW	9	39019451	missense	probably damaging	0.99
R7957:Olfr146	UTSW	9	39019053	missense	probably benign
R8052:Olfr146	UTSW	9	39019487	missense	probably damaging	0.99
R8162:Olfr146	UTSW	9	39018953	missense	probably benign	0.01
R9004:Olfr146	UTSW	9	39019284	missense	probably benign	0.01
R9083:Olfr146	UTSW	9	39018720	missense	probably damaging	1.00
R9584:Olfr146	UTSW	9	39019166	missense	probably damaging	1.00
Z1088:Olfr146	UTSW	9	39018789	missense	probably damaging	1.00
Z1191:Olfr146	UTSW	9	39018933	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCGTAGGATGCTGATAATGATG -3'
(R):5'- GAAATCTCCTACCCTGAGTGC -3'

Sequencing Primer
(F):5'- CGTAGGATGCTGATAATGATGAAAGC -3'
(R):5'- ATCTCCTACCCTGAGTGCATAAC -3'

Posted On

2015-02-18