Mutagenetix > Incidental Mutation

Incidental Mutation 'R3411:Mslnl'

267740

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

040629-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3411 (G1)

Quality Score

104

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 25744517 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 326 (F326L)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098] [ENSMUST00000075884]

AlphaFold

Q8C160

Predicted Effect

probably benign
Transcript: ENSMUST00000047098
AA Change: F326L

PolyPhen 2 Score 0.053 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: F326L

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000075884

SMART Domains

Protein: ENSMUSP00000075279
Gene: ENSMUSG00000063011

Domain	Start	End	E-Value	Type
Pfam:Mesothelin	1	624	N/A	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.1806

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.9%
20x: 93.6%

Validation Efficiency

100% (62/62)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam4	A	T	12: 81,419,822	V675D	possibly damaging	Het
Adamts16	T	C	13: 70,753,226	T911A	probably benign	Het
Adgra1	T	C	7: 139,847,703	F62S	possibly damaging	Het
Adgra3	A	G	5: 50,001,930	V326A	probably damaging	Het
Aff1	T	A	5: 103,754,706	M1K	probably null	Het
AI429214	C	T	8: 36,993,917	S73L	probably benign	Het
Apc	C	A	18: 34,269,259		probably benign	Het
Apcs	T	C	1: 172,894,563	Y72C	probably damaging	Het
Arid4a	T	C	12: 71,061,525		probably benign	Het
Armcx4	C	A	X: 134,691,025	Q561K	probably benign	Het
Atp5o	C	T	16: 91,928,906	R64H	probably damaging	Het
Bank1	G	A	3: 136,247,773		probably benign	Het
Ccdc150	A	G	1: 54,356,773	D805G	probably benign	Het
Ccdc88a	T	A	11: 29,486,006	C10S	probably damaging	Het
Dnah1	A	T	14: 31,269,817	M3076K	possibly damaging	Het
Dnpep	A	G	1: 75,316,626	V33A	probably damaging	Het
Egfem1	C	T	3: 29,583,021	T202I	probably damaging	Het
Fam120b	T	A	17: 15,431,635		probably benign	Het
Frem1	T	C	4: 82,963,179	T1263A	possibly damaging	Het
Gm6802	C	A	12: 19,490,620		noncoding transcript	Het
Golga2	C	A	2: 32,302,942	A424E	probably damaging	Het
Gpat2	G	A	2: 127,428,291	V75M	probably damaging	Het
Grik5	T	C	7: 25,062,972	D198G	probably benign	Het
Hcrtr2	A	G	9: 76,233,008	F333L	probably benign	Het
Hjurp	GT	GTT	1: 88,266,524		probably null	Het
Htr1b	A	C	9: 81,632,041	V171G	probably benign	Het
Kalrn	G	T	16: 34,212,272	D1117E	probably benign	Het
Kcp	T	C	6: 29,482,846	N1408S	possibly damaging	Het
Klhl7	G	T	5: 24,138,321	V212L	probably damaging	Het
Klra17	T	A	6: 129,874,846	N21I	probably damaging	Het
Lrrc8d	A	T	5: 105,826,706		noncoding transcript	Het
Mast2	C	G	4: 116,310,910	E881Q	possibly damaging	Het
Mcm6	T	C	1: 128,351,585	T155A	probably benign	Het
Nlrp4c	A	G	7: 6,092,570	K816E	possibly damaging	Het
Olfr1047	T	C	2: 86,228,642	S110G	probably benign	Het
Olfr1453	G	C	19: 13,028,047	A94G	probably benign	Het
Olfr1510	T	A	14: 52,410,361	R170S	possibly damaging	Het
Olfr494	A	T	7: 108,368,344	I285F	possibly damaging	Het
Otoa	A	G	7: 121,122,043	Q427R	probably damaging	Het
Pcdhb6	A	G	18: 37,335,892	E622G	probably damaging	Het
Pdzd8	A	G	19: 59,345,413	Y59H	probably damaging	Het
Psmc3	A	C	2: 91,055,918	D159A	probably damaging	Het
Ripk4	T	C	16: 97,743,957	T497A	probably benign	Het
Rpn2	C	A	2: 157,290,652	A108E	possibly damaging	Het
Serpina3n	A	G	12: 104,411,277	E263G	possibly damaging	Het
Six4	A	G	12: 73,112,883	F101S	probably damaging	Het
Slc16a4	G	A	3: 107,300,872	E233K	probably benign	Het
Smu1	T	C	4: 40,752,008	T183A	probably benign	Het
Sptb	T	G	12: 76,610,815	K1311Q	possibly damaging	Het
Styxl1	T	C	5: 135,765,764	Y83C	probably damaging	Het
Sypl2	C	T	3: 108,216,729	V166I	possibly damaging	Het
Tal2	A	G	4: 53,785,843	N8S	probably damaging	Het
Tenm4	T	C	7: 96,852,530	Y1314H	probably damaging	Het
Ttn	A	C	2: 76,942,405	N2415K	possibly damaging	Het
Usp53	T	C	3: 122,949,858		probably null	Het
Vmn2r65	T	C	7: 84,946,688	I263V	probably benign	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GCTAGAGACCTTTAAGGCTAGAG -3'
(R):5'- AGTTTCAGTTGGAGCTGAGCC -3'

Sequencing Primer
(F):5'- CTTTAATCCCAGCAATCGGGAGG -3'
(R):5'- GAGCTGAGCCCTCCAGC -3'

Posted On

2015-02-18