Mutagenetix > Incidental Mutations

Incidental Mutation 'R3412:Pla2g4f'

267751

Institutional Source

Beutler Lab

Gene Symbol

Pla2g4f

Ensembl Gene

ENSMUSG00000046971

Gene Name

phospholipase A2, group IVF

Synonyms

4732472I07Rik, Pla2zeta

MMRRC Submission

040630-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R3412 (G1)

Quality Score

188

Status

Validated

Chromosome

Chromosomal Location

120299957-120314165 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 120303106 bp

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 579 (S579G)

Ref Sequence

ENSEMBL: ENSMUSP00000062607 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054651]

Predicted Effect

probably benign
Transcript: ENSMUST00000054651
AA Change: S579G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000062607
Gene: ENSMUSG00000046971
AA Change: S579G

Domain	Start	End	E-Value	Type
C2	45	144	7.51e-11	SMART
PLAc	285	797	1.6e-13	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142183

Meta Mutation Damage Score

0.0934

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 94.8%

Validation Efficiency

98% (40/41)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adarb2	T	C	13: 8,752,618	F643S	probably damaging	Het
Ap2a2	T	A	7: 141,598,776	N105K	probably benign	Het
Arhgef5	A	G	6: 43,273,790	I492V	probably benign	Het
Atp8b4	C	A	2: 126,375,757	W613L	probably damaging	Het
Ccdc162	T	C	10: 41,539,549		probably benign	Het
Diexf	A	C	1: 193,128,502	S64R	possibly damaging	Het
Esp24	T	A	17: 39,038,316	I11N	possibly damaging	Het
Exoc4	A	G	6: 33,265,975	E41G	probably damaging	Het
Eya1	T	A	1: 14,274,209		probably null	Het
Gckr	T	A	5: 31,300,867		probably null	Het
Gm4981	G	A	10: 58,236,353	T13I	possibly damaging	Het
Gria4	T	A	9: 4,513,278	D277V	probably benign	Het
Il18r1	A	T	1: 40,491,067	D318V	probably damaging	Het
Il4i1	T	C	7: 44,836,658	L22P	probably damaging	Het
Inpp5d	C	A	1: 87,668,057	T175N	possibly damaging	Het
Krt90	T	A	15: 101,560,593	L171F	probably damaging	Het
Mthfd1	A	G	12: 76,303,749		probably null	Het
Olfr262	T	C	19: 12,241,590	I24V	probably benign	Het
Olfr561	T	C	7: 102,774,755	L77P	possibly damaging	Het
Olfr635	T	C	7: 103,979,402	L76P	probably damaging	Het
Olfr786	A	T	10: 129,437,307	D165V	probably damaging	Het
Ppef2	T	G	5: 92,228,722	S649R	probably damaging	Het
Prss43	G	C	9: 110,829,464	Q277H	probably damaging	Het
Ramp2	TTGCTGCTGCTGCTGCTGCTGCTG	TTGCTGCTGCTGCTGCTGCTG	11: 101,246,545		probably benign	Het
Rusc2	T	G	4: 43,415,935	S414A	probably damaging	Het
Sez6l	A	G	5: 112,475,361	L108P	possibly damaging	Het
Slc12a5	A	G	2: 164,968,431	D10G	probably benign	Het
Slc1a7	A	G	4: 108,010,994	E497G	probably benign	Het
Sos1	T	C	17: 80,406,717	D1108G	probably benign	Het
Spag8	T	A	4: 43,651,606	S423C	probably damaging	Het
Tacc2	A	G	7: 130,734,994	T2369A	probably benign	Het
Taok2	T	C	7: 126,870,858	I933V	possibly damaging	Het
Tex9	C	A	9: 72,477,758	Q265H	possibly damaging	Het
Trim69	T	C	2: 122,178,644	V395A	probably benign	Het
Tusc1	C	A	4: 93,334,936	R162L	probably damaging	Het
Ubr5	T	C	15: 38,004,235		probably benign	Het
Zfyve28	C	T	5: 34,199,684	M723I	probably benign	Het
Zmynd8	A	T	2: 165,815,451	M533K	probably damaging	Het

Other mutations in Pla2g4f

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00539:Pla2g4f	APN	2	120302738	missense	possibly damaging	0.53
IGL01652:Pla2g4f	APN	2	120302235	missense	possibly damaging	0.86
IGL02792:Pla2g4f	APN	2	120303369	missense	probably damaging	1.00
R0625:Pla2g4f	UTSW	2	120305041	missense	probably damaging	1.00
R1760:Pla2g4f	UTSW	2	120314066	unclassified	probably benign
R1799:Pla2g4f	UTSW	2	120311068	missense	possibly damaging	0.49
R2212:Pla2g4f	UTSW	2	120303106	missense	probably benign
R2351:Pla2g4f	UTSW	2	120300442	missense	probably benign	0.01
R3414:Pla2g4f	UTSW	2	120303106	missense	probably benign
R3906:Pla2g4f	UTSW	2	120300499	missense	probably benign	0.28
R4084:Pla2g4f	UTSW	2	120312325	missense	probably benign	0.36
R4477:Pla2g4f	UTSW	2	120303672	missense	probably damaging	1.00
R4529:Pla2g4f	UTSW	2	120300619	missense	probably damaging	0.99
R4606:Pla2g4f	UTSW	2	120313986	missense	probably benign	0.00
R4685:Pla2g4f	UTSW	2	120305015	missense	probably damaging	1.00
R4728:Pla2g4f	UTSW	2	120300921	missense	probably benign	0.19
R4782:Pla2g4f	UTSW	2	120303276	missense	probably damaging	1.00
R4957:Pla2g4f	UTSW	2	120300499	missense	probably benign	0.28
R5781:Pla2g4f	UTSW	2	120305023	missense	probably damaging	0.97
R6158:Pla2g4f	UTSW	2	120301071	missense	probably benign	0.21
R6232:Pla2g4f	UTSW	2	120302221	missense	possibly damaging	0.63
R6629:Pla2g4f	UTSW	2	120308242	missense	probably damaging	1.00
R6894:Pla2g4f	UTSW	2	120303596	missense	probably benign	0.44
R6939:Pla2g4f	UTSW	2	120307301	missense	probably damaging	1.00
R7131:Pla2g4f	UTSW	2	120304554	missense	probably null	0.01
R7221:Pla2g4f	UTSW	2	120300995	missense	probably benign	0.06
R7421:Pla2g4f	UTSW	2	120307256	missense	probably benign	0.07
R7767:Pla2g4f	UTSW	2	120305009	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GGTCCTGCTTCCGTAACTTTGG -3'
(R):5'- AATTCTTCATGGGCCGGCTG -3'

Sequencing Primer
(F):5'- AACTTTGGCCAGTCATCTAGG -3'
(R):5'- TGCATTTCTGGCCAGAGC -3'

Posted On

2015-02-18