Incidental Mutation 'R3614:Cd3g'
ID 268375
Institutional Source Beutler Lab
Gene Symbol Cd3g
Ensembl Gene ENSMUSG00000002033
Gene Name CD3 antigen, gamma polypeptide
Synonyms Ctg3, T3g, Ctg-3
Accession Numbers
Essential gene? Probably non essential (E-score: 0.078) question?
Stock # R3614 (G1)
Quality Score 225
Status Not validated
Chromosome 9
Chromosomal Location 44880870-44891729 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 44891587 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Serine at position 11 (F11S)
Ref Sequence ENSEMBL: ENSMUSP00000002101 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000002101] [ENSMUST00000034602] [ENSMUST00000160886]
AlphaFold P11942
PDB Structure CD3 Epsilon and gamma Ectodomain Fragment Complex in Single-Chain Construct [SOLUTION NMR]
Predicted Effect probably benign
Transcript: ENSMUST00000002101
AA Change: F11S

PolyPhen 2 Score 0.387 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000002101
Gene: ENSMUSG00000002033
AA Change: F11S

DomainStartEndE-ValueType
IGc2 37 94 3.51e-8 SMART
transmembrane domain 115 137 N/A INTRINSIC
ITAM 157 177 2.06e-3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000034602
SMART Domains Protein: ENSMUSP00000034602
Gene: ENSMUSG00000032094

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
Pfam:Ig_4 30 103 5.9e-23 PFAM
transmembrane domain 105 127 N/A INTRINSIC
ITAM 146 166 2.41e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000160886
AA Change: F11S

PolyPhen 2 Score 0.270 (Sensitivity: 0.91; Specificity: 0.88)
SMART Domains Protein: ENSMUSP00000125151
Gene: ENSMUSG00000002033
AA Change: F11S

DomainStartEndE-ValueType
IGc2 29 86 3.51e-8 SMART
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.0%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit decreased thymocyte number and T cell response. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 13 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adcy10 A G 1: 165,403,296 (GRCm39) Y1598C probably benign Het
Asic4 A G 1: 75,449,702 (GRCm39) D444G probably damaging Het
Dsp A G 13: 38,361,175 (GRCm39) T365A probably damaging Het
Fabp3 C T 4: 130,206,180 (GRCm39) T57I probably benign Het
Golga3 C A 5: 110,368,774 (GRCm39) Q1365K probably damaging Het
Lama3 T A 18: 12,581,345 (GRCm39) H601Q probably benign Het
Lmbrd2 A G 15: 9,177,798 (GRCm39) D499G probably damaging Het
Or2aj6 A G 16: 19,443,515 (GRCm39) C112R probably damaging Het
Otop2 G A 11: 115,219,972 (GRCm39) A271T probably benign Het
Pigp T C 16: 94,165,583 (GRCm39) D113G possibly damaging Het
Prex1 G A 2: 166,451,701 (GRCm39) R256C probably damaging Het
Stxbp2 T A 8: 3,681,196 (GRCm39) V10E possibly damaging Het
Vmn1r67 T C 7: 10,181,356 (GRCm39) Y207H probably damaging Het
Other mutations in Cd3g
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01881:Cd3g APN 9 44,882,566 (GRCm39) missense probably damaging 1.00
IGL02624:Cd3g APN 9 44,885,459 (GRCm39) critical splice donor site probably null
IGL02750:Cd3g APN 9 44,882,608 (GRCm39) unclassified probably benign
IGL03097:Cd3g UTSW 9 44,882,061 (GRCm39) missense probably damaging 1.00
R1711:Cd3g UTSW 9 44,885,640 (GRCm39) missense probably damaging 1.00
R2076:Cd3g UTSW 9 44,885,595 (GRCm39) missense probably damaging 1.00
R4514:Cd3g UTSW 9 44,884,882 (GRCm39) missense possibly damaging 0.93
R5732:Cd3g UTSW 9 44,884,929 (GRCm39) missense possibly damaging 0.89
R6520:Cd3g UTSW 9 44,882,613 (GRCm39) splice site probably null
R7447:Cd3g UTSW 9 44,884,857 (GRCm39) missense probably damaging 1.00
R7776:Cd3g UTSW 9 44,885,459 (GRCm39) critical splice donor site probably null
R9041:Cd3g UTSW 9 44,884,818 (GRCm39) missense possibly damaging 0.93
Predicted Primers PCR Primer
(F):5'- ATGAAATCCTCTCTCCAGCCAG -3'
(R):5'- GGTATTGCCTCTACTCAGAGTCC -3'

Sequencing Primer
(F):5'- TGGTTCTTCCCAGCACAGAG -3'
(R):5'- GAGTCCCAGCTCAATGCATG -3'
Posted On 2015-02-19