Mutagenetix > Incidental Mutation

Incidental Mutation 'R3617:Tmem266'

268480

Institutional Source

Beutler Lab

Gene Symbol

Tmem266

Ensembl Gene

ENSMUSG00000032313

Gene Name

transmembrane protein 266

Synonyms

AI118078

MMRRC Submission

040674-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R3617 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

55234197-55345629 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 55307918 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 148 (V148A)

Ref Sequence

ENSEMBL: ENSMUSP00000034862 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034862] [ENSMUST00000085754]

AlphaFold

Q8BZB3

Predicted Effect

probably damaging
Transcript: ENSMUST00000034862
AA Change: V148A

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000034862
Gene: ENSMUSG00000032313
AA Change: V148A

Domain	Start	End	E-Value	Type
low complexity region	16	27	N/A	INTRINSIC
transmembrane domain	95	117	N/A	INTRINSIC
transmembrane domain	137	159	N/A	INTRINSIC
transmembrane domain	172	191	N/A	INTRINSIC
low complexity region	260	283	N/A	INTRINSIC
low complexity region	298	309	N/A	INTRINSIC
low complexity region	381	396	N/A	INTRINSIC
low complexity region	423	439	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000085754
AA Change: V83A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000082906
Gene: ENSMUSG00000032313
AA Change: V83A

Domain	Start	End	E-Value	Type
transmembrane domain	30	52	N/A	INTRINSIC
transmembrane domain	72	94	N/A	INTRINSIC
low complexity region	195	218	N/A	INTRINSIC
low complexity region	233	244	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130465

Meta Mutation Damage Score

0.1495

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.4%

Validation Efficiency

98% (41/42)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc9	A	G	6: 142,625,015 (GRCm39)	V460A	probably damaging	Het
Aplf	T	C	6: 87,648,865 (GRCm39)	I25V	possibly damaging	Het
Ascc3	A	G	10: 50,494,281 (GRCm39)	T239A	probably benign	Het
Bahd1	G	A	2: 118,753,004 (GRCm39)	R757H	probably damaging	Het
Cbfa2t2	A	T	2: 154,278,904 (GRCm39)		probably benign	Het
Ccdc168	T	C	1: 44,100,114 (GRCm39)	D328G	probably benign	Het
Cdkn1c	C	T	7: 143,013,531 (GRCm39)		probably benign	Het
Cfap46	A	G	7: 139,219,515 (GRCm39)	S1317P	probably benign	Het
Cntln	C	T	4: 84,923,214 (GRCm39)	Q560*	probably null	Het
Cntn2	G	C	1: 132,456,361 (GRCm39)	A161G	probably benign	Het
Crybg1	A	G	10: 43,832,782 (GRCm39)	I1991T	possibly damaging	Het
Cttnbp2	T	C	6: 18,414,189 (GRCm39)	E952G	probably damaging	Het
Dhx36	T	C	3: 62,379,428 (GRCm39)	T887A	possibly damaging	Het
Dhx36	A	G	3: 62,394,481 (GRCm39)	F512L	probably benign	Het
Fthl17f	A	G	X: 8,929,862 (GRCm39)	T153A	probably benign	Het
Gad1-ps	T	A	10: 99,281,260 (GRCm39)		noncoding transcript	Het
Gba2	C	T	4: 43,573,803 (GRCm39)	R163H	probably damaging	Het
Gm10717	A	G	9: 3,025,532 (GRCm39)	Y39C	probably benign	Het
Gm17019	A	T	5: 15,081,081 (GRCm39)	M120K	possibly damaging	Het
Helz2	C	T	2: 180,874,854 (GRCm39)	R1880H	probably damaging	Het
Ifi204	T	C	1: 173,583,283 (GRCm39)	I312V	possibly damaging	Het
Kncn	T	C	4: 115,743,089 (GRCm39)	F55L	probably benign	Het
Kndc1	G	A	7: 139,481,976 (GRCm39)		probably benign	Het
Nat8f3	C	A	6: 85,738,670 (GRCm39)	A31S	probably benign	Het
Ndst4	T	C	3: 125,231,782 (GRCm39)	I117T	probably benign	Het
Ndufa9	G	T	6: 126,826,071 (GRCm39)		probably benign	Het
Nup42	T	C	5: 24,387,325 (GRCm39)	S372P	probably benign	Het
Pde6a	A	G	18: 61,364,575 (GRCm39)		probably benign	Het
Pigw	T	C	11: 84,769,133 (GRCm39)	I65M	probably damaging	Het
Pip	A	G	6: 41,828,871 (GRCm39)	T139A	probably benign	Het
Rel	A	T	11: 23,695,780 (GRCm39)	D202E	probably damaging	Het
Rhot2	A	G	17: 26,059,955 (GRCm39)		probably benign	Het
Sh3rf3	G	A	10: 58,922,685 (GRCm39)	R587Q	possibly damaging	Het
Sim1	T	A	10: 50,785,624 (GRCm39)	M231K	probably damaging	Het
Slc12a9	G	A	5: 137,330,759 (GRCm39)	T47M	probably damaging	Het
Slc4a4	A	G	5: 89,382,663 (GRCm39)	D1036G	probably benign	Het
Tas2r143	A	T	6: 42,377,997 (GRCm39)	I276F	probably benign	Het
Tgfbr3	A	T	5: 107,288,485 (GRCm39)	F392Y	possibly damaging	Het
Ubiad1	A	G	4: 148,520,817 (GRCm39)	I269T	probably benign	Het
Vwc2l	A	G	1: 70,768,041 (GRCm39)		probably null	Het
Zbtb49	G	A	5: 38,357,975 (GRCm39)		probably benign	Het

Other mutations in Tmem266

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01726:Tmem266	APN	9	55,342,486 (GRCm39)	missense	probably benign	0.12
IGL03366:Tmem266	APN	9	55,344,517 (GRCm39)	missense	probably benign	0.06
R0088:Tmem266	UTSW	9	55,344,613 (GRCm39)	missense	probably damaging	1.00
R0418:Tmem266	UTSW	9	55,344,697 (GRCm39)	missense	probably benign	0.06
R0497:Tmem266	UTSW	9	55,288,168 (GRCm39)	splice site	probably null
R0763:Tmem266	UTSW	9	55,322,239 (GRCm39)	missense	probably damaging	1.00
R1657:Tmem266	UTSW	9	55,325,292 (GRCm39)	missense	probably damaging	1.00
R4235:Tmem266	UTSW	9	55,325,391 (GRCm39)	missense	probably damaging	0.99
R4767:Tmem266	UTSW	9	55,288,025 (GRCm39)	missense	probably damaging	1.00
R5587:Tmem266	UTSW	9	55,344,850 (GRCm39)	missense	probably damaging	1.00
R5732:Tmem266	UTSW	9	55,288,120 (GRCm39)	missense	probably damaging	1.00
R5783:Tmem266	UTSW	9	55,305,087 (GRCm39)	missense	probably damaging	1.00
R6193:Tmem266	UTSW	9	55,344,493 (GRCm39)	missense	probably benign	0.01
R6943:Tmem266	UTSW	9	55,284,851 (GRCm39)	start gained	probably benign
R7459:Tmem266	UTSW	9	55,303,883 (GRCm39)	missense	unknown
R9167:Tmem266	UTSW	9	55,322,231 (GRCm39)	missense	probably damaging	1.00
R9502:Tmem266	UTSW	9	55,344,973 (GRCm39)	missense	probably damaging	0.96
X0063:Tmem266	UTSW	9	55,288,033 (GRCm39)	missense	probably benign	0.00
Z1177:Tmem266	UTSW	9	55,344,754 (GRCm39)	missense	possibly damaging	0.70
Z1177:Tmem266	UTSW	9	55,305,051 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTGTTCTAACTCTCTGACCCAG -3'
(R):5'- TGCATGTATGTGTCTGTGAACAC -3'

Sequencing Primer
(F):5'- TGGTTTCCACAAGCACCCAG -3'
(R):5'- ATGTATGTGTCTGTGAACACTTCTTG -3'

Posted On

2015-02-19