Mutagenetix > Incidental Mutations

Incidental Mutation 'R3552:Fcrls'

268977

Institutional Source

Beutler Lab

Gene Symbol

Fcrls

Ensembl Gene

ENSMUSG00000015852

Gene Name

Fc receptor-like S, scavenger receptor

Synonyms

IFGP2, Msr2, Fcrh2, moFcRH2sc, 2810439C17Rik

MMRRC Submission

040669-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R3552 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

87250758-87263738 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 87259410 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 92 (I92T)

Ref Sequence

ENSEMBL: ENSMUSP00000115780 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090986] [ENSMUST00000146512]

AlphaFold

Q9EQY5

Predicted Effect

probably benign
Transcript: ENSMUST00000090986
AA Change: I92T

PolyPhen 2 Score 0.127 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000088508
Gene: ENSMUSG00000015852
AA Change: I92T

Domain	Start	End	E-Value	Type
IG	23	106	1.32e-3	SMART
IGc2	122	186	2.77e-6	SMART
IGc2	226	291	1.09e-4	SMART
IG	315	396	1e-3	SMART
SR	402	503	7.29e-36	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000146512
AA Change: I92T

PolyPhen 2 Score 0.734 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000115780
Gene: ENSMUSG00000015852
AA Change: I92T

Domain	Start	End	E-Value	Type
IG	23	106	1.32e-3	SMART
Pfam:Ig_2	111	176	6.1e-6	PFAM
Pfam:Ig_3	111	176	1.4e-4	PFAM

Meta Mutation Damage Score

0.0972

Coding Region Coverage

1x: 99.3%
3x: 98.5%
10x: 96.8%
20x: 93.4%

Validation Efficiency

100% (66/66)

MGI Phenotype

PHENOTYPE: Female homozygous mutant mice are larger than controls and show increased mean body weight, total tissue mass, lean body mass and total body fat. Homozygous mutant mice eshow a decreased mean percentage of CD8 cells in the peripheral blood. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610009O20Rik	A	G	18: 38,258,365		probably benign	Het
Acaca	T	A	11: 84,261,624	Y866N	probably damaging	Het
Agk	A	G	6: 40,394,681	T371A	probably benign	Het
Akna	T	C	4: 63,398,124	M1V	probably null	Het
Aldh7a1	T	C	18: 56,550,292		probably null	Het
Ankrd26	A	T	6: 118,507,776	L1500H	probably damaging	Het
Atp13a5	T	A	16: 29,310,766	D452V	probably damaging	Het
Bahcc1	C	T	11: 120,276,772	T1333M	possibly damaging	Het
Carmil3	G	T	14: 55,507,402	R1276L	possibly damaging	Het
Ccni	T	C	5: 93,187,761	S173G	probably benign	Het
Chrm2	A	T	6: 36,523,810	I201F	probably damaging	Het
Col16a1	A	G	4: 130,077,041	T618A	probably benign	Het
Dock2	T	C	11: 34,720,960	Y192C	probably benign	Het
Ep400	T	A	5: 110,729,287	E821V	unknown	Het
Esrrg	G	A	1: 188,150,190	V215I	probably benign	Het
Evx1	A	T	6: 52,316,923	S359C	probably damaging	Het
Gal3st1	T	A	11: 3,998,110	F106I	possibly damaging	Het
Gm9944	T	C	4: 144,453,043		probably benign	Het
Hjurp	GT	GTT	1: 88,266,524		probably null	Het
Hrc	G	C	7: 45,336,333	E303Q	possibly damaging	Het
Kcnh1	A	G	1: 192,238,766	N118D	probably damaging	Het
Khdrbs1	A	G	4: 129,720,791	I323T	possibly damaging	Het
Klhdc7b	T	C	15: 89,387,521	Y869H	probably benign	Het
Lrrc4c	T	A	2: 97,629,961	W311R	probably damaging	Het
Megf11	A	G	9: 64,695,463	D862G	possibly damaging	Het
Muc5b	A	G	7: 141,861,335	T2673A	possibly damaging	Het
Muc5b	A	G	7: 141,867,705	S4311G	probably benign	Het
Myo15	C	T	11: 60,509,663	A1767V	possibly damaging	Het
Neo1	T	A	9: 58,893,878	K1140M	probably damaging	Het
Oc90	T	C	15: 65,878,801	Q365R	possibly damaging	Het
Olfr1012	T	C	2: 85,759,893	N161S	possibly damaging	Het
Olfr378	T	C	11: 73,425,852	I44V	probably benign	Het
Olfr48	A	C	2: 89,844,343	M210R	possibly damaging	Het
Oplah	C	T	15: 76,302,094	D734N	possibly damaging	Het
Pbx1	G	A	1: 168,158,793	P411L	possibly damaging	Het
Pcdhga6	G	T	18: 37,708,217	R330L	probably benign	Het
Phox2b	C	A	5: 67,097,656	R150L	probably damaging	Het
Plscr2	A	G	9: 92,290,795	E169G	probably damaging	Het
Ptprn2	A	T	12: 116,888,877	Q518L	probably benign	Het
Rbl1	A	T	2: 157,195,585	I214K	probably benign	Het
Ryr1	T	A	7: 29,056,997	Q3464L	probably damaging	Het
Ryr3	T	A	2: 112,751,787	I2854F	probably damaging	Het
Shtn1	T	C	19: 58,975,038	Y615C	probably benign	Het
Sirt5	A	T	13: 43,383,167	N226Y	probably damaging	Het
Slc30a3	G	A	5: 31,095,078		probably benign	Het
Slc5a4b	A	G	10: 76,081,524	V226A	probably damaging	Het
Slf2	C	A	19: 44,934,951	S68*	probably null	Het
Smyd5	G	A	6: 85,442,211	E292K	probably damaging	Het
Spns1	A	G	7: 126,370,371	V512A	possibly damaging	Het
Sry	T	A	Y: 2,663,141	Q173L	unknown	Het
Ssrp1	C	A	2: 85,044,392	Q519K	probably benign	Het
Tgfbr3	T	C	5: 107,139,839	E498G	probably damaging	Het
Tnrc6b	T	G	15: 80,880,247	L650W	probably damaging	Het
Tnxb	A	T	17: 34,718,721	E3861D	probably damaging	Het
Trbc1	G	T	6: 41,539,645		probably benign	Het
Trpm7	T	A	2: 126,826,710		probably benign	Het
Usp39	G	A	6: 72,337,832	T197I	possibly damaging	Het
Vmn1r38	T	C	6: 66,776,493	H213R	possibly damaging	Het
Washc2	A	G	6: 116,220,568	D168G	probably damaging	Het
Washc4	A	G	10: 83,546,856	I45V	probably benign	Het
Zfp352	A	G	4: 90,225,102	E493G	probably benign	Het
Zfp692	C	T	11: 58,309,428	T170I	possibly damaging	Het
Zfp735	C	A	11: 73,711,241	S337*	probably null	Het

Other mutations in Fcrls

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01568:Fcrls	APN	3	87256679	missense	probably damaging	0.99
IGL01959:Fcrls	APN	3	87259632	missense	probably damaging	0.97
IGL02409:Fcrls	APN	3	87252723	missense	probably benign	0.00
IGL02677:Fcrls	APN	3	87259387	missense	probably benign	0.01
IGL02957:Fcrls	APN	3	87262194	missense	possibly damaging	0.59
IGL02974:Fcrls	APN	3	87257397	missense	possibly damaging	0.89
IGL02992:Fcrls	APN	3	87259466	missense	probably damaging	0.99
BB001:Fcrls	UTSW	3	87259533	missense	probably damaging	0.99
BB011:Fcrls	UTSW	3	87259533	missense	probably damaging	0.99
R0052:Fcrls	UTSW	3	87256778	missense	possibly damaging	0.94
R0052:Fcrls	UTSW	3	87256778	missense	possibly damaging	0.94
R0131:Fcrls	UTSW	3	87258959	missense	possibly damaging	0.90
R1171:Fcrls	UTSW	3	87256860	missense	probably benign	0.24
R1319:Fcrls	UTSW	3	87262177	critical splice donor site	probably null
R1522:Fcrls	UTSW	3	87256707	missense	possibly damaging	0.64
R1696:Fcrls	UTSW	3	87259518	missense	possibly damaging	0.95
R1742:Fcrls	UTSW	3	87259043	missense	possibly damaging	0.76
R2156:Fcrls	UTSW	3	87257341	missense	probably benign	0.43
R2255:Fcrls	UTSW	3	87257348	nonsense	probably null
R2257:Fcrls	UTSW	3	87259621	missense	probably damaging	0.99
R2434:Fcrls	UTSW	3	87256698	missense	probably damaging	1.00
R2680:Fcrls	UTSW	3	87257349	missense	probably damaging	0.99
R4866:Fcrls	UTSW	3	87263466	missense	possibly damaging	0.65
R4883:Fcrls	UTSW	3	87259615	missense	possibly damaging	0.48
R5654:Fcrls	UTSW	3	87257544	missense	probably benign
R5771:Fcrls	UTSW	3	87263468	missense	probably damaging	0.98
R5917:Fcrls	UTSW	3	87256787	missense	probably damaging	0.99
R6349:Fcrls	UTSW	3	87252496	missense	probably damaging	0.99
R6562:Fcrls	UTSW	3	87257328	missense	probably benign
R6954:Fcrls	UTSW	3	87263676	critical splice donor site	probably benign
R7059:Fcrls	UTSW	3	87257340	missense	possibly damaging	0.82
R7188:Fcrls	UTSW	3	87259523	missense	probably benign	0.13
R7201:Fcrls	UTSW	3	87252627	missense	probably damaging	0.99
R7369:Fcrls	UTSW	3	87256701	missense	possibly damaging	0.59
R7431:Fcrls	UTSW	3	87258926	missense	probably damaging	0.99
R7610:Fcrls	UTSW	3	87252697	missense	probably damaging	1.00
R7924:Fcrls	UTSW	3	87259533	missense	probably damaging	0.99
R8018:Fcrls	UTSW	3	87259626	nonsense	probably null
R8280:Fcrls	UTSW	3	87259057	nonsense	probably null
R8981:Fcrls	UTSW	3	87257370	missense	probably damaging	1.00
R9368:Fcrls	UTSW	3	87257599	missense	possibly damaging	0.59

Predicted Primers

PCR Primer
(F):5'- GTGCCTACAGAGCAAGACAC -3'
(R):5'- CACACCGTTCTTATGAAGGAGAC -3'

Sequencing Primer
(F):5'- TGCCTACAGAGCAAGACACACATAC -3'
(R):5'- GACCAAGTAGTTATAAGCTGCAC -3'

Posted On

2015-02-19