Incidental Mutation 'R3605:Gal'
ID 269072
Institutional Source Beutler Lab
Gene Symbol Gal
Ensembl Gene ENSMUSG00000024907
Gene Name galanin and GMAP prepropeptide
Synonyms Galn
MMRRC Submission 040670-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R3605 (G1)
Quality Score 225
Status Validated
Chromosome 19
Chromosomal Location 3459915-3464544 bp(-) (GRCm39)
Type of Mutation splice site (6 bp from exon)
DNA Base Change (assembly) A to G at 3464026 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000025842 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000025842]
AlphaFold P47212
Predicted Effect probably null
Transcript: ENSMUST00000025842
SMART Domains Protein: ENSMUSP00000025842
Gene: ENSMUSG00000024907

DomainStartEndE-ValueType
low complexity region 6 18 N/A INTRINSIC
Galanin 20 124 3.41e-68 SMART
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.5%
  • 10x: 96.8%
  • 20x: 93.3%
Validation Efficiency 95% (40/42)
MGI Phenotype FUNCTION: This gene encodes a neuroendocrine peptide that is principally produced by a subpopulation of lactotrophs in the pituitary gland. The encoded protein is a precursor that is proteolytically processed to generate two mature peptides: galanin and galanin message-associated peptide (GMAP). Mice lacking the encoded protein fail to lactate sufficiently due to abnormalities in the expression of prolactin and lactotroph proliferation, exhibit attenuated chronic neuropathic pain and developmental deficits in the dorsal root ganglion neurons. This gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature proteins. [provided by RefSeq, Jul 2016]
PHENOTYPE: Homozygous inactivation of this gene leads to alterations in neuroendocrine homeostasis, prolactin release, lactotroph number, mammary gland maturation, lactation, susceptibility to neuronal excitotoxicity and induced seizures, sensory neuron developmentand regeneration, and long term potentiation. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 39 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ankrd35 C T 3: 96,589,497 (GRCm39) Q239* probably null Het
Arid4b T C 13: 14,294,826 (GRCm39) V36A probably damaging Het
Art2b T A 7: 101,229,152 (GRCm39) N249I probably benign Het
Bpifb1 T A 2: 154,053,485 (GRCm39) N242K possibly damaging Het
Cd200r1 A G 16: 44,609,939 (GRCm39) T53A possibly damaging Het
Cracr2b C T 7: 141,046,059 (GRCm39) P370S possibly damaging Het
Crb1 T A 1: 139,165,077 (GRCm39) T1016S probably damaging Het
Esrrg A G 1: 187,943,299 (GRCm39) H424R possibly damaging Het
Fgfrl1 C A 5: 108,853,289 (GRCm39) T213K probably damaging Het
Flrt3 T A 2: 140,503,287 (GRCm39) N114Y probably damaging Het
Fsip2 T C 2: 82,815,253 (GRCm39) V3662A probably benign Het
Gabra6 T A 11: 42,205,777 (GRCm39) I359F probably benign Het
Gm10801 G C 2: 98,494,352 (GRCm39) R143T possibly damaging Het
Gm5814 G T 17: 47,721,430 (GRCm39) R48L probably damaging Het
Hcn1 G A 13: 118,111,788 (GRCm39) G584D unknown Het
Iqgap1 T C 7: 80,373,537 (GRCm39) D1484G probably benign Het
Kmt2a G A 9: 44,760,493 (GRCm39) T485M probably damaging Het
Kprp C A 3: 92,731,588 (GRCm39) Q487H unknown Het
Lctl T C 9: 64,040,475 (GRCm39) Y473H probably damaging Het
Lrrc8d G A 5: 105,974,873 (GRCm39) C93Y unknown Het
Mnt T A 11: 74,727,746 (GRCm39) S211T possibly damaging Het
Mtdh T A 15: 34,114,258 (GRCm39) probably benign Het
Nxt1 T C 2: 148,517,399 (GRCm39) W47R probably damaging Het
Or4f59 T A 2: 111,873,168 (GRCm39) I70F probably benign Het
Or5k3 T C 16: 58,969,846 (GRCm39) I211T probably damaging Het
Plekha7 T C 7: 115,763,477 (GRCm39) D313G possibly damaging Het
Ranbp10 A G 8: 106,502,667 (GRCm39) S300P probably benign Het
Rbl1 A T 2: 157,019,153 (GRCm39) F531I probably damaging Het
Rpap2 A G 5: 107,768,395 (GRCm39) D411G probably damaging Het
Sapcd1 A G 17: 35,246,781 (GRCm39) F36L probably damaging Het
Svep1 A T 4: 58,066,542 (GRCm39) S3181T probably benign Het
Tgfbr2 G A 9: 115,938,960 (GRCm39) T314I probably benign Het
Thbs4 G T 13: 92,894,467 (GRCm39) C685* probably null Het
Tk2 A G 8: 104,957,803 (GRCm39) V181A possibly damaging Het
Traf2 T C 2: 25,420,427 (GRCm39) T141A probably benign Het
Ttn C T 2: 76,661,788 (GRCm39) probably null Het
Ube3c A G 5: 29,803,936 (GRCm39) T180A possibly damaging Het
Yif1b C T 7: 28,937,835 (GRCm39) A7V possibly damaging Het
Zfp738 A T 13: 67,819,508 (GRCm39) L151* probably null Het
Other mutations in Gal
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00922:Gal APN 19 3,461,575 (GRCm39) missense probably benign 0.02
R0385:Gal UTSW 19 3,461,171 (GRCm39) missense probably benign 0.13
R4817:Gal UTSW 19 3,461,126 (GRCm39) splice site probably null
R4911:Gal UTSW 19 3,461,590 (GRCm39) missense probably benign 0.15
R5046:Gal UTSW 19 3,461,167 (GRCm39) missense probably damaging 0.99
R5921:Gal UTSW 19 3,460,100 (GRCm39) missense probably damaging 0.99
R6660:Gal UTSW 19 3,460,108 (GRCm39) missense possibly damaging 0.52
R7658:Gal UTSW 19 3,463,309 (GRCm39) missense probably damaging 1.00
X0065:Gal UTSW 19 3,461,181 (GRCm39) missense probably benign 0.15
Predicted Primers PCR Primer
(F):5'- CCACTTTGAACCTGTGTCCAG -3'
(R):5'- GACTTGTCAGGCTCTAGTCC -3'

Sequencing Primer
(F):5'- TGTCCAGGTGGTCCTCAG -3'
(R):5'- AGGCTCTAGTCCTCCTGCG -3'
Posted On 2015-02-19