Mutagenetix > Incidental Mutation

Incidental Mutation 'R3608:Vmn2r95'

269169

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r95

Ensembl Gene

ENSMUSG00000091631

Gene Name

vomeronasal 2, receptor 95

Synonyms

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R3608 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

18644366-18672586 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 18660235 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 216 (V216I)

Ref Sequence

ENSEMBL: ENSMUSP00000156117 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166327] [ENSMUST00000232090] [ENSMUST00000232464]

AlphaFold

A0A338P6T0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000166327
AA Change: V216I

PolyPhen 2 Score 0.467 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000126106
Gene: ENSMUSG00000091631
AA Change: V216I

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	82	462	1.8e-35	PFAM
Pfam:NCD3G	509	562	3.2e-20	PFAM
Pfam:7tm_3	594	830	3.2e-51	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000232090
AA Change: V216I

PolyPhen 2 Score 0.467 (Sensitivity: 0.89; Specificity: 0.90)

Predicted Effect

probably benign
Transcript: ENSMUST00000232464
AA Change: V216I

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

Coding Region Coverage

1x: 99.3%
3x: 98.5%
10x: 96.8%
20x: 93.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 23 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
B3gnt4	C	A	5: 123,648,838 (GRCm39)	R68S	probably damaging	Het
Brf1	A	G	12: 112,924,894 (GRCm39)	L610P	probably benign	Het
Cacna1e	T	A	1: 154,291,831 (GRCm39)	R1783S	probably benign	Het
Cdh15	G	A	8: 123,588,763 (GRCm39)	R279Q	probably damaging	Het
CK137956	A	T	4: 127,845,119 (GRCm39)	I208N	probably damaging	Het
Col17a1	G	A	19: 47,668,844 (GRCm39)	L127F	probably benign	Het
Col1a2	G	A	6: 4,518,822 (GRCm39)		probably benign	Het
Dlgap4	C	T	2: 156,590,332 (GRCm39)		probably benign	Het
Ect2l	A	T	10: 18,018,688 (GRCm39)	N619K	possibly damaging	Het
Hamp2	T	C	7: 30,623,539 (GRCm39)	T8A	probably benign	Het
Lamb1	T	A	12: 31,337,909 (GRCm39)	N407K	probably damaging	Het
Lrrc32	C	T	7: 98,148,393 (GRCm39)	T391M	probably benign	Het
Mroh2a	G	T	1: 88,172,717 (GRCm39)	A829S	probably damaging	Het
Ncoa1	T	C	12: 4,328,186 (GRCm39)	N884S	probably benign	Het
Neil1	T	C	9: 57,051,485 (GRCm39)	T278A	probably damaging	Het
Pcnx2	T	C	8: 126,614,840 (GRCm39)	T204A	probably benign	Het
Psmc3	T	A	2: 90,884,925 (GRCm39)	D30E	probably benign	Het
Rtkn	T	A	6: 83,127,016 (GRCm39)	C328S	probably damaging	Het
Speer4f2	C	A	5: 17,579,492 (GRCm39)	T97K	probably benign	Het
Srd5a2	A	G	17: 74,334,026 (GRCm39)	V131A	probably benign	Het
Tm9sf4	A	G	2: 153,020,897 (GRCm39)	H35R	probably benign	Het
Ubr2	G	T	17: 47,255,449 (GRCm39)	D1412E	probably damaging	Het
Ypel1	A	T	16: 16,910,154 (GRCm39)	C126*	probably null	Het

Other mutations in Vmn2r95

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01024:Vmn2r95	APN	17	18,672,590 (GRCm39)	utr 3 prime	probably benign
IGL01479:Vmn2r95	APN	17	18,664,124 (GRCm39)	missense	probably damaging	1.00
IGL01890:Vmn2r95	APN	17	18,671,737 (GRCm39)	missense	probably damaging	1.00
IGL01986:Vmn2r95	APN	17	18,660,473 (GRCm39)	missense	probably benign	0.06
IGL02113:Vmn2r95	APN	17	18,660,169 (GRCm39)	missense	possibly damaging	0.47
IGL02154:Vmn2r95	APN	17	18,672,248 (GRCm39)	missense	probably benign	0.16
IGL02190:Vmn2r95	APN	17	18,672,038 (GRCm39)	missense	probably benign	0.00
IGL02412:Vmn2r95	APN	17	18,660,218 (GRCm39)	missense	probably damaging	1.00
IGL02550:Vmn2r95	APN	17	18,671,994 (GRCm39)	missense	probably damaging	1.00
IGL02679:Vmn2r95	APN	17	18,664,116 (GRCm39)	missense	probably damaging	1.00
IGL02691:Vmn2r95	APN	17	18,672,120 (GRCm39)	missense	probably benign	0.07
IGL02990:Vmn2r95	APN	17	18,672,298 (GRCm39)	nonsense	probably null
IGL03032:Vmn2r95	APN	17	18,672,575 (GRCm39)	missense	probably benign	0.00
R0416:Vmn2r95	UTSW	17	18,661,664 (GRCm39)	missense	probably damaging	1.00
R0448:Vmn2r95	UTSW	17	18,672,005 (GRCm39)	missense	possibly damaging	0.92
R0514:Vmn2r95	UTSW	17	18,671,844 (GRCm39)	missense	probably benign
R0519:Vmn2r95	UTSW	17	18,659,765 (GRCm39)	missense	probably damaging	1.00
R0539:Vmn2r95	UTSW	17	18,672,362 (GRCm39)	missense	probably damaging	1.00
R1501:Vmn2r95	UTSW	17	18,660,118 (GRCm39)	missense	probably damaging	0.99
R1598:Vmn2r95	UTSW	17	18,672,575 (GRCm39)	missense	probably benign	0.03
R1613:Vmn2r95	UTSW	17	18,660,901 (GRCm39)	splice site	probably benign
R1861:Vmn2r95	UTSW	17	18,672,530 (GRCm39)	missense	probably damaging	1.00
R1921:Vmn2r95	UTSW	17	18,644,575 (GRCm39)	missense	probably benign	0.11
R1986:Vmn2r95	UTSW	17	18,671,805 (GRCm39)	missense	probably benign
R2031:Vmn2r95	UTSW	17	18,659,717 (GRCm39)	missense	possibly damaging	0.94
R2040:Vmn2r95	UTSW	17	18,661,561 (GRCm39)	missense	probably damaging	1.00
R3727:Vmn2r95	UTSW	17	18,661,744 (GRCm39)	nonsense	probably null
R3953:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R3955:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R3957:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R4474:Vmn2r95	UTSW	17	18,672,507 (GRCm39)	missense	probably damaging	1.00
R4672:Vmn2r95	UTSW	17	18,672,413 (GRCm39)	missense	probably damaging	1.00
R4850:Vmn2r95	UTSW	17	18,671,915 (GRCm39)	missense	probably damaging	1.00
R5054:Vmn2r95	UTSW	17	18,671,708 (GRCm39)	missense	possibly damaging	0.63
R5178:Vmn2r95	UTSW	17	18,660,337 (GRCm39)	missense	probably benign	0.01
R5980:Vmn2r95	UTSW	17	18,661,624 (GRCm39)	missense	probably benign
R6183:Vmn2r95	UTSW	17	18,664,192 (GRCm39)	missense	probably damaging	0.99
R6276:Vmn2r95	UTSW	17	18,671,732 (GRCm39)	missense	possibly damaging	0.96
R6651:Vmn2r95	UTSW	17	18,660,622 (GRCm39)	missense	probably damaging	1.00
R6682:Vmn2r95	UTSW	17	18,660,489 (GRCm39)	missense	probably damaging	1.00
R6797:Vmn2r95	UTSW	17	18,672,551 (GRCm39)	utr 3 prime	probably benign
R6799:Vmn2r95	UTSW	17	18,659,555 (GRCm39)	missense	probably damaging	1.00
R6849:Vmn2r95	UTSW	17	18,664,182 (GRCm39)	missense	probably damaging	1.00
R6849:Vmn2r95	UTSW	17	18,664,181 (GRCm39)	missense	probably damaging	1.00
R6982:Vmn2r95	UTSW	17	18,672,323 (GRCm39)	missense	probably damaging	1.00
R7203:Vmn2r95	UTSW	17	18,661,577 (GRCm39)	missense	probably benign	0.01
R7226:Vmn2r95	UTSW	17	18,672,245 (GRCm39)	missense	possibly damaging	0.90
R7240:Vmn2r95	UTSW	17	18,672,225 (GRCm39)	missense	probably benign	0.15
R7383:Vmn2r95	UTSW	17	18,660,734 (GRCm39)	missense	probably benign	0.06
R7614:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R7755:Vmn2r95	UTSW	17	18,644,367 (GRCm39)	start codon destroyed	probably null	0.99
R7942:Vmn2r95	UTSW	17	18,660,529 (GRCm39)	missense	possibly damaging	0.74
R8355:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R8455:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R8478:Vmn2r95	UTSW	17	18,672,544 (GRCm39)	missense	probably damaging	1.00
R8547:Vmn2r95	UTSW	17	18,664,161 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r95	UTSW	17	18,661,738 (GRCm39)	missense	probably damaging	0.98
R8788:Vmn2r95	UTSW	17	18,671,790 (GRCm39)	missense	probably benign	0.09
R8852:Vmn2r95	UTSW	17	18,664,113 (GRCm39)	missense	possibly damaging	0.95
R9098:Vmn2r95	UTSW	17	18,660,167 (GRCm39)	missense	possibly damaging	0.88
R9202:Vmn2r95	UTSW	17	18,644,394 (GRCm39)	missense	probably benign	0.00
R9244:Vmn2r95	UTSW	17	18,672,189 (GRCm39)	missense	possibly damaging	0.91
R9546:Vmn2r95	UTSW	17	18,661,721 (GRCm39)	missense	probably benign	0.01
R9665:Vmn2r95	UTSW	17	18,660,607 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r95	UTSW	17	18,660,663 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TAAGTCCTTGAAACTTAGAGGCC -3'
(R):5'- ACCTTCTAGTGAATCAATGTCACC -3'

Sequencing Primer
(F):5'- ATTCTCTTGTCTTCTATTCCTTCAGG -3'
(R):5'- GAAGTCCATGTTTCTGAGG -3'

Posted On

2015-02-19