Mutagenetix > Incidental Mutation

Incidental Mutation 'R3683:Vmn1r8'

269421

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r8

Ensembl Gene

ENSMUSG00000061208

Gene Name

vomeronasal 1 receptor 8

Synonyms

V1rc32

MMRRC Submission

040681-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R3683 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

57012898-57014110 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 57013260 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 104 (T104P)

Ref Sequence

ENSEMBL: ENSMUSP00000154298 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078186] [ENSMUST00000227706] [ENSMUST00000228690]

AlphaFold

Q8R2C2

Predicted Effect

probably damaging
Transcript: ENSMUST00000078186
AA Change: T104P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000093561
Gene: ENSMUSG00000061208
AA Change: T104P

Domain	Start	End	E-Value	Type
Pfam:V1R	28	293	1.2e-58	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000227706
AA Change: T104P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

probably damaging
Transcript: ENSMUST00000228690
AA Change: T104P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930595M18Rik	T	C	X: 80,463,841 (GRCm39)	D622G	possibly damaging	Het
Acad11	T	C	9: 103,992,543 (GRCm39)	I495T	probably damaging	Het
Acat1	C	T	9: 53,498,765 (GRCm39)	A307T	probably damaging	Het
Ahdc1	T	C	4: 132,793,013 (GRCm39)	L1418P	possibly damaging	Het
Bmp2	T	A	2: 133,396,392 (GRCm39)	V16D	probably benign	Het
Cables2	G	A	2: 179,906,263 (GRCm39)	R121*	probably null	Het
Calb2	A	G	8: 110,883,620 (GRCm39)	Y35H	probably benign	Het
Cdon	A	G	9: 35,400,328 (GRCm39)	E1014G	possibly damaging	Het
Clcn7	T	C	17: 25,369,567 (GRCm39)	L301P	possibly damaging	Het
Corin	T	C	5: 72,488,198 (GRCm39)	D610G	probably damaging	Het
Csrnp2	A	T	15: 100,379,879 (GRCm39)	S471T	probably benign	Het
Dnah7a	C	A	1: 53,483,675 (GRCm39)	M3367I	probably benign	Het
Elp4	A	T	2: 105,533,106 (GRCm39)	M413K	possibly damaging	Het
Fat1	A	G	8: 45,470,975 (GRCm39)	D1593G	probably benign	Het
Fbxw24	G	A	9: 109,437,110 (GRCm39)	P259L	possibly damaging	Het
Ggta1	T	A	2: 35,298,000 (GRCm39)	T162S	probably benign	Het
Gldn	G	A	9: 54,245,624 (GRCm39)	E392K	possibly damaging	Het
Klra9	T	C	6: 130,168,260 (GRCm39)	N2S	probably benign	Het
Lama1	A	G	17: 68,075,328 (GRCm39)	D1015G	probably benign	Het
Lct	T	C	1: 128,231,963 (GRCm39)	M629V	probably damaging	Het
Myg1	G	C	15: 102,246,171 (GRCm39)	G349R	probably damaging	Het
Nrxn1	T	C	17: 90,930,880 (GRCm39)	D779G	probably damaging	Het
Or2d3c	G	A	7: 106,526,301 (GRCm39)	R122*	probably null	Het
Or4a73	C	A	2: 89,421,099 (GRCm39)	R120L	probably damaging	Het
Or4c118	A	T	2: 88,975,364 (GRCm39)	M1K	probably null	Het
Or5d37	T	A	2: 87,923,603 (GRCm39)	I226F	probably damaging	Het
Osmr	A	C	15: 6,866,534 (GRCm39)	V380G	possibly damaging	Het
Phf21b	A	G	15: 84,682,891 (GRCm39)	I222T	probably damaging	Het
Ptprq	G	T	10: 107,544,489 (GRCm39)	T347K	probably benign	Het
Sema6d	G	T	2: 124,496,146 (GRCm39)	L63F	possibly damaging	Het
Serpinb9b	C	T	13: 33,213,581 (GRCm39)	A46V	probably damaging	Het
Sf3b3	A	G	8: 111,540,253 (GRCm39)		probably null	Het
Smpd1	T	A	7: 105,204,609 (GRCm39)	C163S	probably damaging	Het
Stat4	C	T	1: 52,052,981 (GRCm39)	T54I	possibly damaging	Het
Tacc2	A	G	7: 130,226,800 (GRCm39)	S1162G	probably benign	Het
Tbc1d20	C	T	2: 152,153,737 (GRCm39)	T354I	probably benign	Het
Tbc1d31	T	C	15: 57,815,210 (GRCm39)		probably null	Het
Tmcc1	C	CAT	6: 116,019,831 (GRCm39)		probably null	Het
Tmem181a	T	A	17: 6,346,061 (GRCm39)	L185H	probably damaging	Het
Unc79	T	A	12: 103,041,062 (GRCm39)	N698K	probably benign	Het
Utrn	A	G	10: 12,542,579 (GRCm39)	M1802T	probably benign	Het
Vmn2r13	C	A	5: 109,304,721 (GRCm39)	R570I	probably damaging	Het
Zfand6	G	A	7: 84,283,570 (GRCm39)	P11S	probably damaging	Het
Zfp1005	A	G	2: 150,109,976 (GRCm39)	K222R	probably benign	Het
Zmpste24	A	G	4: 120,918,288 (GRCm39)	F445L	probably damaging	Het

Other mutations in Vmn1r8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01569:Vmn1r8	APN	6	57,013,272 (GRCm39)	missense	possibly damaging	0.70
IGL01820:Vmn1r8	APN	6	57,013,653 (GRCm39)	missense	possibly damaging	0.94
IGL02416:Vmn1r8	APN	6	57,013,605 (GRCm39)	missense	probably damaging	0.97
IGL02496:Vmn1r8	APN	6	57,013,556 (GRCm39)	missense	probably damaging	1.00
IGL02930:Vmn1r8	APN	6	57,013,579 (GRCm39)	missense	probably benign	0.03
IGL02954:Vmn1r8	APN	6	57,013,315 (GRCm39)	missense	probably benign	0.03
IGL03235:Vmn1r8	APN	6	57,013,746 (GRCm39)	nonsense	probably null
IGL03353:Vmn1r8	APN	6	57,013,776 (GRCm39)	missense	probably benign	0.03
PIT4494001:Vmn1r8	UTSW	6	57,013,712 (GRCm39)	missense	probably benign	0.01
R0656:Vmn1r8	UTSW	6	57,013,573 (GRCm39)	missense	probably benign	0.35
R1328:Vmn1r8	UTSW	6	57,013,278 (GRCm39)	missense	possibly damaging	0.94
R1846:Vmn1r8	UTSW	6	57,013,413 (GRCm39)	missense	probably benign	0.06
R2083:Vmn1r8	UTSW	6	57,013,325 (GRCm39)	missense	probably benign	0.21
R4134:Vmn1r8	UTSW	6	57,013,705 (GRCm39)	missense	probably benign
R4754:Vmn1r8	UTSW	6	57,012,952 (GRCm39)	start codon destroyed	probably null	1.00
R4857:Vmn1r8	UTSW	6	57,013,338 (GRCm39)	missense	probably benign	0.00
R5787:Vmn1r8	UTSW	6	57,013,244 (GRCm39)	missense	probably damaging	1.00
R6158:Vmn1r8	UTSW	6	57,013,274 (GRCm39)	missense	probably benign	0.00
R6711:Vmn1r8	UTSW	6	57,013,444 (GRCm39)	missense	probably damaging	1.00
R6971:Vmn1r8	UTSW	6	57,013,400 (GRCm39)	missense	probably damaging	1.00
R8066:Vmn1r8	UTSW	6	57,013,419 (GRCm39)	missense	probably benign	0.03
R8261:Vmn1r8	UTSW	6	57,013,158 (GRCm39)	missense	probably benign	0.00
R8526:Vmn1r8	UTSW	6	57,013,362 (GRCm39)	missense	probably benign	0.05
R8552:Vmn1r8	UTSW	6	57,013,138 (GRCm39)	missense	possibly damaging	0.76
R8692:Vmn1r8	UTSW	6	57,013,557 (GRCm39)	missense	probably benign	0.01
R8712:Vmn1r8	UTSW	6	57,013,665 (GRCm39)	missense	probably benign	0.07
R9306:Vmn1r8	UTSW	6	57,013,713 (GRCm39)	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- GTCAACTGTCCTTTGTGCAC -3'
(R):5'- AATAAGTCCCCTGGTGATGTGG -3'

Sequencing Primer
(F):5'- CACATAATGATGGTTCTCATTGGAGG -3'
(R):5'- AGTGAGCAGGATTTAGTGACC -3'

Posted On

2015-02-19