Incidental Mutation 'R3686:Zfp446'
ID 269522
Institutional Source Beutler Lab
Gene Symbol Zfp446
Ensembl Gene ENSMUSG00000033961
Gene Name zinc finger protein 446
Synonyms A630035I11Rik
Accession Numbers
Essential gene? Probably non essential (E-score: 0.080) question?
Stock # R3686 (G1)
Quality Score 225
Status Not validated
Chromosome 7
Chromosomal Location 12711726-12718323 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 12716580 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Asparagine at position 517 (I517N)
Ref Sequence ENSEMBL: ENSMUSP00000039073 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000045810] [ENSMUST00000108535] [ENSMUST00000108536] [ENSMUST00000108537]
AlphaFold Q8C9M8
Predicted Effect probably damaging
Transcript: ENSMUST00000045810
AA Change: I517N

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000039073
Gene: ENSMUSG00000033961
AA Change: I517N

DomainStartEndE-ValueType
low complexity region 67 79 N/A INTRINSIC
SCAN 122 234 1.29e-53 SMART
KRAB 299 360 3.96e-2 SMART
ZnF_C2H2 419 441 2.95e-3 SMART
ZnF_C2H2 468 490 8.47e-4 SMART
ZnF_C2H2 496 518 5.34e-1 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108535
SMART Domains Protein: ENSMUSP00000104175
Gene: ENSMUSG00000033961

DomainStartEndE-ValueType
SCAN 22 134 1.29e-53 SMART
KRAB 199 254 1.14e0 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108536
AA Change: I417N

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000104176
Gene: ENSMUSG00000033961
AA Change: I417N

DomainStartEndE-ValueType
SCAN 22 134 1.29e-53 SMART
KRAB 199 260 3.96e-2 SMART
ZnF_C2H2 319 341 2.95e-3 SMART
ZnF_C2H2 368 390 8.47e-4 SMART
ZnF_C2H2 396 418 5.34e-1 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000108537
AA Change: I342N

PolyPhen 2 Score 0.630 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000104177
Gene: ENSMUSG00000033961
AA Change: I342N

DomainStartEndE-ValueType
SCAN 22 128 7.37e-49 SMART
KRAB 124 185 3.96e-2 SMART
ZnF_C2H2 244 266 2.95e-3 SMART
ZnF_C2H2 293 315 8.47e-4 SMART
ZnF_C2H2 321 343 5.34e-1 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000128427
Predicted Effect noncoding transcript
Transcript: ENSMUST00000147996
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.2%
  • 20x: 94.8%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 34 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Asb16 T A 11: 102,159,885 (GRCm39) D79E probably benign Het
Atp4a G A 7: 30,419,650 (GRCm39) R671Q probably benign Het
B230307C23Rik T A 16: 97,810,199 (GRCm39) N62K probably benign Het
B3galnt2 G A 13: 14,150,220 (GRCm39) probably null Het
Bnip2 T A 9: 69,906,432 (GRCm39) Y118N probably damaging Het
Bptf C A 11: 106,965,024 (GRCm39) R1275L probably benign Het
Cacna1g T C 11: 94,349,916 (GRCm39) probably null Het
Cdh15 G A 8: 123,588,763 (GRCm39) R279Q probably damaging Het
Cdh4 T C 2: 179,422,160 (GRCm39) S95P probably benign Het
Ceacam5 G A 7: 17,494,748 (GRCm39) E919K possibly damaging Het
Eftud2 T C 11: 102,735,027 (GRCm39) E624G probably damaging Het
Hr T A 14: 70,795,236 (GRCm39) N289K probably damaging Het
Hsp90ab1 A G 17: 45,880,214 (GRCm39) Y110H probably damaging Het
Map3k1 A G 13: 111,890,425 (GRCm39) V1258A probably damaging Het
Naa11 A T 5: 97,539,648 (GRCm39) V170E probably benign Het
Nmd3 C T 3: 69,654,095 (GRCm39) R413C probably damaging Het
Or4k51 G T 2: 111,584,914 (GRCm39) V107F probably benign Het
Or56b2 A G 7: 104,337,599 (GRCm39) I126V probably benign Het
Pgm3 G T 9: 86,441,563 (GRCm39) P345T probably benign Het
Ptpn14 C A 1: 189,583,596 (GRCm39) D814E probably damaging Het
Rab36 G A 10: 74,880,328 (GRCm39) V63I probably damaging Het
Rap1gap2 C A 11: 74,298,148 (GRCm39) A491S possibly damaging Het
Ros1 A T 10: 52,021,912 (GRCm39) V624E probably damaging Het
Sgo2b T C 8: 64,384,361 (GRCm39) K212E probably benign Het
Shisa2 T A 14: 59,867,228 (GRCm39) L160Q probably damaging Het
Strn4 A G 7: 16,556,506 (GRCm39) Y123C probably damaging Het
Sycp2 T C 2: 178,016,177 (GRCm39) T762A probably benign Het
Tiam2 A G 17: 3,471,959 (GRCm39) K534E possibly damaging Het
Trdn A T 10: 33,344,185 (GRCm39) D633V probably benign Het
Ttn T C 2: 76,747,333 (GRCm39) Y4572C possibly damaging Het
Unc79 T A 12: 103,054,920 (GRCm39) N921K probably damaging Het
Vmn2r66 A G 7: 84,644,397 (GRCm39) V671A probably damaging Het
Wdr86 T A 5: 24,923,339 (GRCm39) T118S probably damaging Het
Zfp830 G A 11: 82,656,188 (GRCm39) E331K possibly damaging Het
Other mutations in Zfp446
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01486:Zfp446 APN 7 12,713,307 (GRCm39) critical splice donor site probably null
IGL01534:Zfp446 APN 7 12,713,493 (GRCm39) missense probably damaging 1.00
IGL02471:Zfp446 APN 7 12,716,181 (GRCm39) missense probably benign 0.41
IGL02888:Zfp446 APN 7 12,713,255 (GRCm39) missense probably damaging 1.00
IGL03153:Zfp446 APN 7 12,711,834 (GRCm39) missense probably benign 0.01
IGL03185:Zfp446 APN 7 12,712,925 (GRCm39) missense probably null 0.43
IGL03242:Zfp446 APN 7 12,713,252 (GRCm39) missense probably damaging 1.00
R0538:Zfp446 UTSW 7 12,713,516 (GRCm39) missense possibly damaging 0.94
R1809:Zfp446 UTSW 7 12,713,048 (GRCm39) missense probably damaging 1.00
R3701:Zfp446 UTSW 7 12,712,079 (GRCm39) unclassified probably benign
R5256:Zfp446 UTSW 7 12,713,231 (GRCm39) nonsense probably null
R5363:Zfp446 UTSW 7 12,711,984 (GRCm39) missense probably benign 0.20
R5377:Zfp446 UTSW 7 12,716,178 (GRCm39) missense possibly damaging 0.88
R6806:Zfp446 UTSW 7 12,713,043 (GRCm39) missense probably damaging 1.00
R7167:Zfp446 UTSW 7 12,712,049 (GRCm39) unclassified probably benign
R7988:Zfp446 UTSW 7 12,712,970 (GRCm39) missense possibly damaging 0.93
R8288:Zfp446 UTSW 7 12,711,885 (GRCm39) missense probably benign 0.00
R8487:Zfp446 UTSW 7 12,716,555 (GRCm39) missense possibly damaging 0.68
R8943:Zfp446 UTSW 7 12,713,564 (GRCm39) nonsense probably null
R9315:Zfp446 UTSW 7 12,713,397 (GRCm39) missense probably benign 0.28
Z1177:Zfp446 UTSW 7 12,712,093 (GRCm39) unclassified probably benign
Predicted Primers PCR Primer
(F):5'- TGGAGAGACCATCACAGGTG -3'
(R):5'- AAGGGACTAGCCTCTTCTCCTTAC -3'

Sequencing Primer
(F):5'- ACCATCACAGGTGGCCCG -3'
(R):5'- CTCCTTACACTGGATAGTCTGGTG -3'
Posted On 2015-02-19