Mutagenetix > Incidental Mutation

Incidental Mutation 'R1839:Muc4'

269781

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

045015-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R1839 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

32735886-32782391 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to C at 32753919 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Proline at position 1265 (R1265P)

Ref Sequence

ENSEMBL: ENSMUSP00000093813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106] [ENSMUST00000132475]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000096106
AA Change: R1265P

PolyPhen 2 Score 0.100 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: R1265P

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000132475

SMART Domains

Protein: ENSMUSP00000119029
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	38	45	N/A	INTRINSIC
low complexity region	72	84	N/A	INTRINSIC
low complexity region	99	127	N/A	INTRINSIC
low complexity region	148	173	N/A	INTRINSIC
low complexity region	192	224	N/A	INTRINSIC
low complexity region	283	293	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135753

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142355

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.4%
3x: 96.7%
10x: 94.7%
20x: 90.6%

Validation Efficiency

98% (90/92)

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	T	C	3: 124,409,720 (GRCm38)	T403A	probably damaging	Het
Acat3	T	A	17: 12,928,606 (GRCm38)	R175*	probably null	Het
Adam1b	C	A	5: 121,501,041 (GRCm38)	C647F	probably damaging	Het
Adam28	T	C	14: 68,639,210 (GRCm38)	N197S	possibly damaging	Het
Adcy2	C	T	13: 68,689,261 (GRCm38)		probably null	Het
Adcy5	A	T	16: 35,248,940 (GRCm38)	N426I	probably damaging	Het
Adgre1	T	C	17: 57,441,299 (GRCm38)	S500P	probably benign	Het
Aloxe3	A	G	11: 69,130,085 (GRCm38)	Y212C	probably damaging	Het
Ap3d1	A	G	10: 80,727,108 (GRCm38)	S180P	probably damaging	Het
Arhgap20	C	T	9: 51,849,326 (GRCm38)	R790W	probably damaging	Het
Atp8b4	C	A	2: 126,361,782 (GRCm38)	A757S	possibly damaging	Het
Begain	G	A	12: 109,035,323 (GRCm38)		probably benign	Het
Ccdc141	T	C	2: 77,011,665 (GRCm38)	E1474G	probably benign	Het
Ccdc88b	A	G	19: 6,854,109 (GRCm38)		probably benign	Het
Ccnk	A	G	12: 108,195,074 (GRCm38)	T195A	probably damaging	Het
Cd55b	C	A	1: 130,414,105 (GRCm38)	C265F	probably damaging	Het
Celsr3	A	G	9: 108,829,906 (GRCm38)	H1196R	probably benign	Het
Cenpt	T	C	8: 105,849,014 (GRCm38)	S190G	possibly damaging	Het
Chd8	T	C	14: 52,204,883 (GRCm38)	S2077G	probably benign	Het
Col6a5	G	A	9: 105,864,833 (GRCm38)	H2296Y	probably benign	Het
Cxxc4	C	A	3: 134,240,653 (GRCm38)	H332N	probably damaging	Het
Cyp24a1	T	C	2: 170,496,741 (GRCm38)	I12V	probably benign	Het
Cyp3a57	T	A	5: 145,381,301 (GRCm38)	L364Q	probably damaging	Het
Ddi2	T	C	4: 141,713,526 (GRCm38)	I47V	probably benign	Het
Ddx5	A	T	11: 106,784,897 (GRCm38)	D322E	probably benign	Het
Dhx40	A	G	11: 86,789,297 (GRCm38)	C405R	possibly damaging	Het
Emc1	T	C	4: 139,360,485 (GRCm38)	F100S	probably damaging	Het
Exoc2	T	C	13: 30,906,497 (GRCm38)		probably benign	Het
Gm10110	A	T	14: 89,897,836 (GRCm38)		noncoding transcript	Het
Gm17332	T	C	11: 31,182,386 (GRCm38)	H26R	possibly damaging	Het
Gna12	T	C	5: 140,762,612 (GRCm38)	N183S	probably benign	Het
Gpx6	A	G	13: 21,312,327 (GRCm38)	N24D	probably benign	Het
Gsdma3	C	T	11: 98,629,858 (GRCm38)	A105V	probably benign	Het
Hsd3b5	T	C	3: 98,619,728 (GRCm38)	Y134C	probably benign	Het
Ifi213	T	C	1: 173,589,600 (GRCm38)	I415M	probably damaging	Het
Ints9	C	A	14: 65,016,530 (GRCm38)	P278T	probably damaging	Het
Krt79	C	T	15: 101,937,938 (GRCm38)	E192K	possibly damaging	Het
Lrrk2	A	G	15: 91,683,134 (GRCm38)	N132S	probably benign	Het
Ltn1	T	A	16: 87,416,264 (GRCm38)	K470*	probably null	Het
Magi2	A	T	5: 20,465,827 (GRCm38)	T163S	probably damaging	Het
Mcm9	A	G	10: 53,541,553 (GRCm38)	M18T	probably damaging	Het
Med12l	A	G	3: 59,068,319 (GRCm38)	T212A	probably benign	Het
Mfhas1	T	C	8: 35,590,858 (GRCm38)	L829P	possibly damaging	Het
Mgme1	T	A	2: 144,279,487 (GRCm38)	C288S	probably benign	Het
Myh7	T	C	14: 54,973,180 (GRCm38)	N1725S	possibly damaging	Het
Naa80	G	A	9: 107,583,017 (GRCm38)	R37H	possibly damaging	Het
Nme4	A	T	17: 26,092,097 (GRCm38)	W165R	probably damaging	Het
Nup205	T	A	6: 35,219,714 (GRCm38)	D1128E	probably benign	Het
Or2t48	A	T	11: 58,529,373 (GRCm38)	Y204*	probably null	Het
Or5ae2	T	C	7: 84,856,548 (GRCm38)	Y60H	probably damaging	Het
Pcdh1	T	C	18: 38,199,485 (GRCm38)	D155G	possibly damaging	Het
Pex12	A	T	11: 83,297,822 (GRCm38)	S116T	probably damaging	Het
Plekhh1	C	T	12: 79,078,957 (GRCm38)		probably benign	Het
Plekhh3	A	G	11: 101,163,600 (GRCm38)		probably benign	Het
Pnpt1	T	C	11: 29,154,342 (GRCm38)	M572T	possibly damaging	Het
Ppp1r12b	T	C	1: 134,837,981 (GRCm38)	R667G	probably benign	Het
Rbpms2	ACTGCTGCTGCTGCTGC	ACTGCTGCTGCTGCTGCTGC	9: 65,651,666 (GRCm38)		probably benign	Het
Rgs14	T	C	13: 55,382,838 (GRCm38)		probably benign	Het
Rhbdf2	A	T	11: 116,600,191 (GRCm38)	V645E	possibly damaging	Het
Robo3	A	G	9: 37,422,327 (GRCm38)	V696A	probably benign	Het
Sall1	A	G	8: 89,028,716 (GRCm38)	F1212L	possibly damaging	Het
Sdc4	T	C	2: 164,429,012 (GRCm38)	E109G	probably benign	Het
Serpind1	G	T	16: 17,342,992 (GRCm38)	R462L	probably damaging	Het
Smad9	CTTT	CTT	3: 54,789,179 (GRCm38)		probably benign	Het
Sstr4	C	A	2: 148,395,533 (GRCm38)	N21K	probably benign	Het
Tap1	C	T	17: 34,188,109 (GRCm38)	A77V	possibly damaging	Het
Thap4	T	C	1: 93,750,287 (GRCm38)	E259G	probably benign	Het
Thra	A	G	11: 98,756,143 (GRCm38)	N30S	probably benign	Het
Tmem207	A	T	16: 26,524,821 (GRCm38)	V27E	possibly damaging	Het
Top3a	A	G	11: 60,753,888 (GRCm38)	V305A	probably damaging	Het
Trim59	A	T	3: 69,037,638 (GRCm38)	I123K	probably damaging	Het
Ttn	T	C	2: 76,861,495 (GRCm38)		probably benign	Het
Ubac1	T	A	2: 26,007,738 (GRCm38)	E290V	possibly damaging	Het
Unc13b	T	C	4: 43,258,308 (GRCm38)		probably benign	Het
Uri1	A	T	7: 37,967,389 (GRCm38)	D206E	probably benign	Het
Utp4	A	G	8: 106,913,454 (GRCm38)	H465R	probably benign	Het
Uvssa	T	C	5: 33,389,752 (GRCm38)	S221P	probably benign	Het
Vmn1r39	T	C	6: 66,805,233 (GRCm38)		probably null	Het
Vps39	A	T	2: 120,325,397 (GRCm38)	L514H	probably damaging	Het
Vps72	G	A	3: 95,119,218 (GRCm38)	R158Q	possibly damaging	Het
Wdr59	T	C	8: 111,485,340 (GRCm38)	D366G	probably benign	Het
Zfp366	C	A	13: 99,228,492 (GRCm38)	Q54K	probably damaging	Het
Zfp523	T	A	17: 28,194,993 (GRCm38)	I34N	probably damaging	Het
Zfp974	G	A	7: 27,910,356 (GRCm38)	P648L	possibly damaging	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,778,812 (GRCm38)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,753,185 (GRCm38)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,750,910 (GRCm38)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,754,347 (GRCm38)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,849 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,848 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,823 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,755,805 (GRCm38)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,753,955 (GRCm38)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,754,071 (GRCm38)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,763,966 (GRCm38)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,750,655 (GRCm38)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,756,627 (GRCm38)	missense	unknown
IGL01625:Muc4	APN	16	32,755,544 (GRCm38)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,736,402 (GRCm38)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,761,348 (GRCm38)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,753,196 (GRCm38)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,751,426 (GRCm38)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,754,265 (GRCm38)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,777,649 (GRCm38)	splice site	probably benign
IGL02210:Muc4	APN	16	32,752,254 (GRCm38)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,750,850 (GRCm38)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,752,076 (GRCm38)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,756,129 (GRCm38)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,775,819 (GRCm38)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,755,282 (GRCm38)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,751,648 (GRCm38)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,750,394 (GRCm38)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,752,048 (GRCm38)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,751,948 (GRCm38)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,751,439 (GRCm38)	nonsense	probably null
IGL03335:Muc4	APN	16	32,753,021 (GRCm38)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,754,318 (GRCm38)	missense	probably benign	0.01
multisplendored	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,770,394 (GRCm38)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,760,251 (GRCm38)	splice site	probably benign
BB004:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
BB014:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
IGL02835:Muc4	UTSW	16	32,763,945 (GRCm38)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,760,248 (GRCm38)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,754,796 (GRCm38)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,756,017 (GRCm38)	missense	unknown
R0119:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,771,604 (GRCm38)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,765,746 (GRCm38)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,752,905 (GRCm38)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,762,536 (GRCm38)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,751,069 (GRCm38)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,756,746 (GRCm38)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,752,107 (GRCm38)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,751,670 (GRCm38)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,769,827 (GRCm38)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,769,847 (GRCm38)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,778,955 (GRCm38)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,756,196 (GRCm38)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,750,525 (GRCm38)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,753,020 (GRCm38)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,752,233 (GRCm38)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,750,349 (GRCm38)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,755,501 (GRCm38)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,756,902 (GRCm38)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,755,933 (GRCm38)	unclassified	probably benign
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,756,251 (GRCm38)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,756,341 (GRCm38)	missense	unknown
R1928:Muc4	UTSW	16	32,750,532 (GRCm38)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,750,642 (GRCm38)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,751,303 (GRCm38)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,752,254 (GRCm38)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,756,409 (GRCm38)	missense	unknown
R2121:Muc4	UTSW	16	32,760,238 (GRCm38)	missense	unknown
R2139:Muc4	UTSW	16	32,761,225 (GRCm38)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,750,476 (GRCm38)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,766,942 (GRCm38)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,755,891 (GRCm38)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,757,472 (GRCm38)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,755,273 (GRCm38)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,755,826 (GRCm38)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,770,292 (GRCm38)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,756,701 (GRCm38)	missense	unknown
R4523:Muc4	UTSW	16	32,736,336 (GRCm38)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,755,843 (GRCm38)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,757,058 (GRCm38)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,769,277 (GRCm38)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,755,846 (GRCm38)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,751,214 (GRCm38)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,775,903 (GRCm38)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,753,625 (GRCm38)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,751,747 (GRCm38)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,756,098 (GRCm38)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,754,319 (GRCm38)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,041 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,756,296 (GRCm38)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,757,058 (GRCm38)	nonsense	probably null
R5161:Muc4	UTSW	16	32,762,521 (GRCm38)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,751,738 (GRCm38)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,751,666 (GRCm38)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,761,261 (GRCm38)	missense	unknown
R5567:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,750,782 (GRCm38)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,776,807 (GRCm38)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,736,241 (GRCm38)	nonsense	probably null
R5708:Muc4	UTSW	16	32,754,769 (GRCm38)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,751,916 (GRCm38)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,774,839 (GRCm38)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,751,295 (GRCm38)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,751,705 (GRCm38)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,753,052 (GRCm38)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,756,278 (GRCm38)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,759,308 (GRCm38)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,775,783 (GRCm38)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,756,795 (GRCm38)	missense	unknown
R6144:Muc4	UTSW	16	32,766,924 (GRCm38)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,736,140 (GRCm38)	start gained	probably benign
R6268:Muc4	UTSW	16	32,768,767 (GRCm38)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,752,035 (GRCm38)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,753,946 (GRCm38)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,754,358 (GRCm38)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,767,351 (GRCm38)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,736,243 (GRCm38)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,778,946 (GRCm38)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,751,789 (GRCm38)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,759,320 (GRCm38)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,782,008 (GRCm38)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,766,938 (GRCm38)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,775,803 (GRCm38)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,762,518 (GRCm38)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,751,999 (GRCm38)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,751,810 (GRCm38)	nonsense	probably null
R7033:Muc4	UTSW	16	32,756,324 (GRCm38)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,757,091 (GRCm38)	missense
R7123:Muc4	UTSW	16	32,750,691 (GRCm38)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,762,488 (GRCm38)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,756,461 (GRCm38)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,755,336 (GRCm38)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,755,366 (GRCm38)	missense	unknown
R7381:Muc4	UTSW	16	32,780,915 (GRCm38)	missense
R7411:Muc4	UTSW	16	32,751,322 (GRCm38)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,766,950 (GRCm38)	missense
R7539:Muc4	UTSW	16	32,756,396 (GRCm38)	missense
R7544:Muc4	UTSW	16	32,736,198 (GRCm38)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,765,702 (GRCm38)	missense
R7596:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,752,361 (GRCm38)	nonsense	probably null
R7639:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,760,105 (GRCm38)	missense
R7651:Muc4	UTSW	16	32,756,575 (GRCm38)	missense
R7688:Muc4	UTSW	16	32,751,460 (GRCm38)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,753,011 (GRCm38)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,768,734 (GRCm38)	missense
R7763:Muc4	UTSW	16	32,753,311 (GRCm38)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,756,194 (GRCm38)	missense	unknown
R7787:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,751,321 (GRCm38)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
R7930:Muc4	UTSW	16	32,754,078 (GRCm38)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,756,749 (GRCm38)	missense
R8096:Muc4	UTSW	16	32,755,390 (GRCm38)	missense	unknown
R8115:Muc4	UTSW	16	32,755,304 (GRCm38)	missense	unknown
R8162:Muc4	UTSW	16	32,750,379 (GRCm38)	missense	unknown
R8220:Muc4	UTSW	16	32,755,327 (GRCm38)	missense	unknown
R8260:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,754,316 (GRCm38)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,755,897 (GRCm38)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,752,401 (GRCm38)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,753,508 (GRCm38)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,752,993 (GRCm38)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,755,255 (GRCm38)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,781,986 (GRCm38)	missense
R8845:Muc4	UTSW	16	32,756,515 (GRCm38)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,750,643 (GRCm38)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,751,462 (GRCm38)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8929:Muc4	UTSW	16	32,753,994 (GRCm38)	missense	probably benign	0.00
R8990:Muc4	UTSW	16	32,752,209 (GRCm38)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,762,536 (GRCm38)	missense
R9034:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,757,468 (GRCm38)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,768,728 (GRCm38)	missense
R9336:Muc4	UTSW	16	32,750,938 (GRCm38)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,751,153 (GRCm38)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,756,618 (GRCm38)	missense
R9429:Muc4	UTSW	16	32,755,724 (GRCm38)	missense	unknown
R9570:Muc4	UTSW	16	32,750,877 (GRCm38)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,756,860 (GRCm38)	missense
R9696:Muc4	UTSW	16	32,753,284 (GRCm38)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,770,270 (GRCm38)	missense
R9743:Muc4	UTSW	16	32,780,824 (GRCm38)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,751,858 (GRCm38)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,751,825 (GRCm38)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,755,481 (GRCm38)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,759,319 (GRCm38)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,756,076 (GRCm38)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,767,393 (GRCm38)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,774,862 (GRCm38)	missense
Z1177:Muc4	UTSW	16	32,767,394 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- GGTCTCACCTTCATCCCAGAAC -3'
(R):5'- CTCATGAGGGAGCATGTGAG -3'

Sequencing Primer
(F):5'- GAACATGTCAACAGTGTCAGC -3'
(R):5'- GGTGTAGAACCTTGAGTCCTTCC -3'

Posted On

2015-03-12