Mutagenetix > Incidental Mutation

Incidental Mutation 'R3739:Zfp362'

270277

Institutional Source

Beutler Lab

Gene Symbol

Zfp362

Ensembl Gene

ENSMUSG00000028799

Gene Name

zinc finger protein 362

Synonyms

MMRRC Submission

040725-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.296) question?

Stock #

R3739 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

128773088-128806045 bp(-) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

C to G at 128786889 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000128499 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071108] [ENSMUST00000106072] [ENSMUST00000170934]

AlphaFold

B1ASA5

Predicted Effect

probably benign
Transcript: ENSMUST00000071108

SMART Domains

Protein: ENSMUSP00000065733
Gene: ENSMUSG00000028799

Domain	Start	End	E-Value	Type
low complexity region	1	14	N/A	INTRINSIC
low complexity region	128	150	N/A	INTRINSIC
low complexity region	200	211	N/A	INTRINSIC
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.98e-4	SMART
ZnF_C2H2	281	303	1.38e-3	SMART
ZnF_C2H2	309	333	3.49e-5	SMART
ZnF_C2H2	339	361	1.08e-1	SMART
ZnF_C2H2	369	391	7.26e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000106072

SMART Domains

Protein: ENSMUSP00000101686
Gene: ENSMUSG00000028799

Domain	Start	End	E-Value	Type
low complexity region	1	14	N/A	INTRINSIC
low complexity region	128	150	N/A	INTRINSIC
low complexity region	200	211	N/A	INTRINSIC
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.98e-4	SMART
ZnF_C2H2	281	303	1.38e-3	SMART
ZnF_C2H2	309	333	3.49e-5	SMART
ZnF_C2H2	339	361	1.08e-1	SMART
ZnF_C2H2	369	391	7.26e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138371

Predicted Effect

probably benign
Transcript: ENSMUST00000170934

SMART Domains

Protein: ENSMUSP00000128499
Gene: ENSMUSG00000028799

Domain	Start	End	E-Value	Type
low complexity region	115	137	N/A	INTRINSIC
low complexity region	187	198	N/A	INTRINSIC
ZnF_C2H2	212	234	2.75e-3	SMART
ZnF_C2H2	240	262	1.98e-4	SMART
ZnF_C2H2	268	290	1.38e-3	SMART
ZnF_C2H2	296	320	3.49e-5	SMART
ZnF_C2H2	326	348	1.08e-1	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

98% (49/50)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930111J21Rik2	A	T	11: 49,019,281	L775*	probably null	Het
Abcb8	A	G	5: 24,400,621	S168G	probably benign	Het
Ahnak2	T	C	12: 112,774,558	I1027V	probably benign	Het
Alox12e	A	G	11: 70,319,842	L318P	probably damaging	Het
Ankrd11	A	G	8: 122,896,715		probably benign	Het
Anks1b	T	A	10: 90,033,216	I46N	probably damaging	Het
Apoa5	G	C	9: 46,269,117	W7S	probably damaging	Het
Arnt2	T	C	7: 84,343,801	H177R	probably null	Het
Cacna1c	T	C	6: 118,741,952	D220G	probably benign	Het
Dst	C	T	1: 34,268,894		probably benign	Het
Eml6	C	A	11: 29,803,137	V925L	probably benign	Het
Fbll1	G	A	11: 35,797,678	H253Y	possibly damaging	Het
Galnt1	A	G	18: 24,271,655	T350A	probably benign	Het
Gbp10	T	A	5: 105,224,458	E145D	possibly damaging	Het
Gfm1	T	C	3: 67,456,700	I503T	probably damaging	Het
Hmcn2	A	T	2: 31,336,612	K200*	probably null	Het
Ifi203	T	A	1: 173,929,474		probably benign	Het
Itgbl1	T	C	14: 123,966,678	F394L	probably damaging	Het
Itpkc	T	A	7: 27,227,604	D295V	possibly damaging	Het
Klra17	T	A	6: 129,873,365	I41F	probably benign	Het
Lcorl	A	T	5: 45,734,041	N323K	possibly damaging	Het
Ltbp2	A	T	12: 84,804,474	C836S	probably damaging	Het
Mfsd2b	A	G	12: 4,870,578	S80P	probably damaging	Het
Ms4a18	A	T	19: 11,011,499	H164Q	probably damaging	Het
Mybbp1a	A	G	11: 72,448,737	H882R	possibly damaging	Het
Myh9	G	A	15: 77,766,812	R1612C	probably damaging	Het
Myo18a	A	G	11: 77,845,615	D1514G	probably damaging	Het
Nsun2	T	C	13: 69,629,638	I441T	probably benign	Het
Ntng1	A	T	3: 109,934,691	D255E	probably damaging	Het
Olfr1385	A	G	11: 49,495,460	D309G	possibly damaging	Het
Osbpl2	G	A	2: 180,161,560	R475H	probably damaging	Het
Pclo	A	C	5: 14,680,899	K3138N	unknown	Het
Pcsk7	A	G	9: 45,926,759	T572A	possibly damaging	Het
Pex11a	C	T	7: 79,740,170	R56H	possibly damaging	Het
Pnmal2	C	T	7: 16,946,596	H502Y	probably benign	Het
Saa4	T	A	7: 46,729,629	N96Y	possibly damaging	Het
Serpinb6d	T	A	13: 33,667,680	V140E	probably damaging	Het
Srsf4	C	T	4: 131,900,102		probably benign	Het
St18	G	A	1: 6,855,473		probably null	Het
Taf3	T	C	2: 9,951,658	E566G	possibly damaging	Het
Tnr	T	C	1: 159,923,413	S1315P	possibly damaging	Het
Trappc11	T	C	8: 47,514,103	E412G	probably damaging	Het
Trpc2	G	A	7: 102,084,504	S220N	probably damaging	Het
Trpm7	A	G	2: 126,851,521	V48A	probably damaging	Het
Tubb6	A	G	18: 67,402,051	Y340C	probably damaging	Het
Vmn1r197	C	A	13: 22,328,576	Y222*	probably null	Het
Vmn2r97	T	C	17: 18,928,151	S103P	probably damaging	Het
Zfp423	A	G	8: 87,781,344	C666R	probably damaging	Het

Other mutations in Zfp362

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01667:Zfp362	APN	4	128787109	missense	probably damaging	1.00
IGL01939:Zfp362	APN	4	128787053	missense	probably benign
trek	UTSW	4	128785976	missense	probably damaging	1.00
Zax	UTSW	4	128790260	missense	possibly damaging	0.71
Zebulon	UTSW	4	128786015	missense	probably damaging	1.00
R1471:Zfp362	UTSW	4	128787200	missense	probably benign
R1892:Zfp362	UTSW	4	128790264	missense	probably benign	0.17
R1901:Zfp362	UTSW	4	128790276	missense	probably damaging	1.00
R2058:Zfp362	UTSW	4	128786987	missense	possibly damaging	0.71
R6772:Zfp362	UTSW	4	128790260	missense	possibly damaging	0.71
R6900:Zfp362	UTSW	4	128786015	missense	probably damaging	1.00
R6901:Zfp362	UTSW	4	128786015	missense	probably damaging	1.00
R7105:Zfp362	UTSW	4	128774526	missense	probably damaging	0.99
R7348:Zfp362	UTSW	4	128777217	missense	possibly damaging	0.88
R7629:Zfp362	UTSW	4	128786055	missense	probably damaging	1.00
R7712:Zfp362	UTSW	4	128777410	missense	probably benign	0.14
R7763:Zfp362	UTSW	4	128787031	missense	probably benign
R8434:Zfp362	UTSW	4	128785976	missense	probably damaging	1.00
R8508:Zfp362	UTSW	4	128774606	missense	probably damaging	1.00
R9432:Zfp362	UTSW	4	128777187	missense	probably damaging	1.00
R9567:Zfp362	UTSW	4	128794888	start codon destroyed	unknown
Z1177:Zfp362	UTSW	4	128774550	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GTTTTAACACGGCTCAGGAGGAC -3'
(R):5'- CACTCCTGGACTCCATCAAG -3'

Sequencing Primer
(F):5'- AGAGTCCTGCCTGCCATG -3'
(R):5'- TGGACTCCATCAAGACAATCCAGG -3'

Posted On

2015-03-18