Incidental Mutation 'R3723:Kctd16'
ID270649
Institutional Source Beutler Lab
Gene Symbol Kctd16
Ensembl Gene ENSMUSG00000051401
Gene Namepotassium channel tetramerisation domain containing 16
Synonyms4930434H12Rik, LOC383347
MMRRC Submission 040714-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.118) question?
Stock #R3723 (G1)
Quality Score225
Status Validated
Chromosome18
Chromosomal Location40258361-40531168 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 40258859 bp
ZygosityHeterozygous
Amino Acid Change Threonine to Alanine at position 167 (T167A)
Ref Sequence ENSEMBL: ENSMUSP00000089547 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000091927] [ENSMUST00000096572]
Predicted Effect possibly damaging
Transcript: ENSMUST00000091927
AA Change: T167A

PolyPhen 2 Score 0.790 (Sensitivity: 0.85; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000089547
Gene: ENSMUSG00000051401
AA Change: T167A

DomainStartEndE-ValueType
BTB 25 130 8.7e-7 SMART
low complexity region 288 300 N/A INTRINSIC
low complexity region 374 396 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000096572
SMART Domains Protein: ENSMUSP00000094323
Gene: ENSMUSG00000071860

DomainStartEndE-ValueType
transmembrane domain 99 121 N/A INTRINSIC
Meta Mutation Damage Score 0.2196 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.4%
  • 20x: 95.4%
Validation Efficiency 95% (38/40)
MGI Phenotype PHENOTYPE: Homozygous knockout leads to reduced extinction or increase of fear memory in cued or contextual conditioning behavior tests, respectively. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 40 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ahnak T C 19: 9,016,853 V5167A possibly damaging Het
Ano5 A G 7: 51,576,528 Y510C probably damaging Het
Api5 C T 2: 94,425,613 R243Q possibly damaging Het
Apob A T 12: 8,006,327 Q1570L probably damaging Het
Apob A G 12: 8,011,763 N3415S possibly damaging Het
Arsk T A 13: 76,066,653 I361F probably damaging Het
C9 G A 15: 6,483,080 E228K possibly damaging Het
Celsr2 A G 3: 108,397,415 probably benign Het
Cfap53 A G 18: 74,359,569 I455V probably benign Het
Ckap4 A G 10: 84,528,392 L269P probably damaging Het
Dnah7a T A 1: 53,447,346 D3352V probably benign Het
Dync2h1 A T 9: 7,041,658 M3335K probably benign Het
Fam171a1 A G 2: 3,220,375 probably benign Het
Glis3 A T 19: 28,262,591 C97* probably null Het
Gm13101 T C 4: 143,966,681 T76A probably benign Het
Gm14221 C G 2: 160,568,427 noncoding transcript Het
Gm8603 A C 17: 13,516,813 probably null Het
Gm9767 G T 10: 26,078,571 probably benign Het
Kif18b A G 11: 102,916,276 F78L probably damaging Het
Mefv A G 16: 3,708,194 probably null Het
Mipol1 C A 12: 57,457,092 L349I probably damaging Het
Myo10 T C 15: 25,803,288 V1527A probably damaging Het
Naip5 G T 13: 100,223,014 Y571* probably null Het
Npl A G 1: 153,515,464 F182L probably benign Het
Pan3 T A 5: 147,503,208 probably benign Het
Pcdh15 A T 10: 74,645,848 T342S probably benign Het
Pcdhga1 A G 18: 37,662,992 T350A possibly damaging Het
Prdm4 A G 10: 85,899,281 S666P probably damaging Het
Rere A G 4: 150,468,795 E148G probably damaging Het
Scube2 T C 7: 109,808,406 probably benign Het
Sptbn1 A G 11: 30,137,335 S1035P possibly damaging Het
Srsf4 C T 4: 131,900,102 probably benign Het
Supt3 A G 17: 44,994,387 D108G probably damaging Het
Tnfsf13b A G 8: 10,031,545 I236V possibly damaging Het
Tns1 T C 1: 73,924,940 S1511G probably damaging Het
Tpm1 G A 9: 67,031,945 probably benign Het
Uba6 T C 5: 86,135,047 D559G probably damaging Het
Vmn1r117 T A 7: 20,883,455 I223F probably damaging Het
Vmn2r91 G A 17: 18,085,278 probably null Het
Zfp30 A G 7: 29,793,353 E344G probably damaging Het
Other mutations in Kctd16
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00954:Kctd16 APN 18 40258800 missense probably benign 0.01
IGL02001:Kctd16 APN 18 40258680 missense possibly damaging 0.48
IGL02173:Kctd16 APN 18 40530853 missense probably benign 0.01
IGL03112:Kctd16 APN 18 40258800 missense probably benign 0.08
R0109:Kctd16 UTSW 18 40259151 missense probably benign 0.06
R0109:Kctd16 UTSW 18 40259151 missense probably benign 0.06
R0267:Kctd16 UTSW 18 40530877 missense probably benign 0.02
R0554:Kctd16 UTSW 18 40258439 missense probably benign
R0732:Kctd16 UTSW 18 40258563 missense probably damaging 1.00
R0883:Kctd16 UTSW 18 40530775 missense probably damaging 0.98
R2140:Kctd16 UTSW 18 40259178 missense possibly damaging 0.89
R2141:Kctd16 UTSW 18 40259178 missense possibly damaging 0.89
R2142:Kctd16 UTSW 18 40259178 missense possibly damaging 0.89
R2160:Kctd16 UTSW 18 40259085 missense probably damaging 1.00
R3724:Kctd16 UTSW 18 40258859 missense possibly damaging 0.79
R5483:Kctd16 UTSW 18 40530876 missense probably benign
R5589:Kctd16 UTSW 18 40259008 missense probably damaging 1.00
R5767:Kctd16 UTSW 18 40258869 missense probably benign 0.13
R5811:Kctd16 UTSW 18 40258452 missense probably damaging 1.00
R5911:Kctd16 UTSW 18 40530852 missense probably benign 0.11
R5930:Kctd16 UTSW 18 40530829 missense probably benign 0.10
R6017:Kctd16 UTSW 18 40258943 missense probably damaging 1.00
R6603:Kctd16 UTSW 18 40258491 missense probably benign
R7404:Kctd16 UTSW 18 40258773 missense probably damaging 1.00
R7597:Kctd16 UTSW 18 40530795 missense possibly damaging 0.70
Predicted Primers PCR Primer
(F):5'- AAGCTGAGTACTTCCAGCTCCC -3'
(R):5'- GAGTTACAGGCCACCATGTG -3'

Sequencing Primer
(F):5'- CTGACCTCGTCAAACTCCTGG -3'
(R):5'- GCTCTTTCCAGATGTTTAAACTTGAG -3'
Posted On2015-03-18