Incidental Mutation 'R3725:Vmn2r54'
ID 270730
Institutional Source Beutler Lab
Gene Symbol Vmn2r54
Ensembl Gene ENSMUSG00000096593
Gene Name vomeronasal 2, receptor 54
Synonyms
MMRRC Submission 040716-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.135) question?
Stock # R3725 (G1)
Quality Score 225
Status Validated
Chromosome 7
Chromosomal Location 12349160-12374167 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 12366223 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Isoleucine at position 237 (T237I)
Ref Sequence ENSEMBL: ENSMUSP00000083386 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000086210]
AlphaFold A0A3B2W422
Predicted Effect probably benign
Transcript: ENSMUST00000086210
AA Change: T237I

PolyPhen 2 Score 0.418 (Sensitivity: 0.89; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000083386
Gene: ENSMUSG00000096593
AA Change: T237I

DomainStartEndE-ValueType
Pfam:ANF_receptor 5 397 4.3e-58 PFAM
Pfam:NCD3G 442 495 2.2e-19 PFAM
Pfam:7tm_3 526 763 1.2e-54 PFAM
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.1%
  • 20x: 94.5%
Validation Efficiency 96% (55/57)
Allele List at MGI
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam8 T A 7: 139,563,781 (GRCm39) R740S possibly damaging Het
Adamtsl3 A C 7: 82,261,612 (GRCm39) D1676A possibly damaging Het
Atp6v1a A G 16: 43,922,120 (GRCm39) probably benign Het
Camsap3 T C 8: 3,653,785 (GRCm39) L485P probably damaging Het
Ccdc81 A T 7: 89,515,838 (GRCm39) F614I possibly damaging Het
Cdk5rap2 C A 4: 70,153,674 (GRCm39) K1716N possibly damaging Het
Cfap47 T A X: 78,553,621 (GRCm39) T285S probably damaging Het
Cfh T C 1: 140,014,234 (GRCm39) M1197V probably damaging Het
Cyp3a11 A G 5: 145,802,810 (GRCm39) F228L probably benign Het
Ddx24 A G 12: 103,383,864 (GRCm39) M575T probably benign Het
Dhx36 A T 3: 62,395,643 (GRCm39) probably benign Het
Dmxl2 T A 9: 54,301,053 (GRCm39) I1554L probably damaging Het
Dsp A G 13: 38,378,665 (GRCm39) probably null Het
Dsp A G 13: 38,381,594 (GRCm39) S2181G probably benign Het
Epg5 T C 18: 78,060,894 (GRCm39) I1959T probably benign Het
Fam135a T A 1: 24,096,515 (GRCm39) K77* probably null Het
Fam209 T C 2: 172,315,915 (GRCm39) S97P probably benign Het
Fbxo11 A G 17: 88,316,714 (GRCm39) V323A probably benign Het
Fzd5 A G 1: 64,775,498 (GRCm39) S88P probably damaging Het
Galnt12 A T 4: 47,104,140 (GRCm39) T133S probably damaging Het
Gja8 A G 3: 96,827,161 (GRCm39) L167P probably damaging Het
Gm8730 T C 8: 103,591,664 (GRCm39) noncoding transcript Het
Gsdmd A G 15: 75,737,939 (GRCm39) D247G probably benign Het
Iqcg A G 16: 32,840,909 (GRCm39) probably null Het
Lamb1 C T 12: 31,371,074 (GRCm39) A1375V probably null Het
Mlip A G 9: 77,097,662 (GRCm39) S282P probably damaging Het
Nfxl1 A T 5: 72,674,405 (GRCm39) D831E probably damaging Het
Nipbl T C 15: 8,325,145 (GRCm39) D2506G probably damaging Het
Or6c212 A T 10: 129,558,984 (GRCm39) V143D probably damaging Het
Or7a39 T A 10: 78,715,766 (GRCm39) Y253* probably null Het
Pcdhb9 T C 18: 37,534,654 (GRCm39) L216P possibly damaging Het
Pigc G A 1: 161,798,860 (GRCm39) G281R possibly damaging Het
Polr3g C T 13: 81,842,754 (GRCm39) R87H probably damaging Het
Ppfia4 T C 1: 134,241,449 (GRCm39) D502G probably benign Het
Psg18 T A 7: 18,088,748 (GRCm39) probably benign Het
Rad9a G A 19: 4,247,694 (GRCm39) R179C probably damaging Het
Rxra A G 2: 27,644,289 (GRCm39) D327G probably damaging Het
Samd7 T C 3: 30,805,283 (GRCm39) V22A possibly damaging Het
Slc22a29 A T 19: 8,195,973 (GRCm39) V22D possibly damaging Het
Slmap C A 14: 26,148,397 (GRCm39) R671S probably damaging Het
Smarcal1 T C 1: 72,665,755 (GRCm39) F751S possibly damaging Het
Smarcb1 T A 10: 75,752,620 (GRCm39) K73N probably benign Het
Sptssa T C 12: 54,703,180 (GRCm39) E30G probably damaging Het
Stpg2 A G 3: 139,023,238 (GRCm39) K418R probably benign Het
Tasor2 T C 13: 3,640,538 (GRCm39) I200V probably benign Het
Tmem19 A G 10: 115,195,675 (GRCm39) probably benign Het
Tmem59l A G 8: 70,939,951 (GRCm39) L6S unknown Het
Tmod1 T C 4: 46,097,026 (GRCm39) V273A probably benign Het
Tnrc6c C T 11: 117,614,355 (GRCm39) R838W probably damaging Het
Uggt1 A T 1: 36,221,588 (GRCm39) L43* probably null Het
Vmn1r205 A T 13: 22,776,671 (GRCm39) F144I probably damaging Het
Vmn2r7 A T 3: 64,632,412 (GRCm39) F17I possibly damaging Het
Vpreb3 G A 10: 75,779,125 (GRCm39) probably null Het
Vps13d T A 4: 144,842,218 (GRCm39) probably benign Het
Zkscan5 A T 5: 145,157,723 (GRCm39) R742W probably damaging Het
Other mutations in Vmn2r54
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00155:Vmn2r54 APN 7 12,365,840 (GRCm39) splice site probably benign
IGL01778:Vmn2r54 APN 7 12,366,009 (GRCm39) missense probably benign 0.07
IGL01998:Vmn2r54 APN 7 12,349,227 (GRCm39) missense probably benign
IGL02028:Vmn2r54 APN 7 12,366,088 (GRCm39) missense probably damaging 1.00
IGL02064:Vmn2r54 APN 7 12,349,533 (GRCm39) missense probably benign 0.02
IGL02238:Vmn2r54 APN 7 12,369,910 (GRCm39) missense probably damaging 1.00
IGL03062:Vmn2r54 APN 7 12,366,355 (GRCm39) missense probably damaging 0.98
IGL03120:Vmn2r54 APN 7 12,349,314 (GRCm39) missense probably damaging 1.00
PIT4453001:Vmn2r54 UTSW 7 12,363,669 (GRCm39) missense probably benign 0.06
R0212:Vmn2r54 UTSW 7 12,366,424 (GRCm39) missense probably benign
R0360:Vmn2r54 UTSW 7 12,349,576 (GRCm39) missense probably damaging 1.00
R1646:Vmn2r54 UTSW 7 12,366,434 (GRCm39) missense probably damaging 1.00
R1673:Vmn2r54 UTSW 7 12,350,138 (GRCm39) critical splice acceptor site probably null
R1738:Vmn2r54 UTSW 7 12,369,815 (GRCm39) missense probably benign 0.00
R1856:Vmn2r54 UTSW 7 12,366,238 (GRCm39) missense probably benign
R2012:Vmn2r54 UTSW 7 12,349,804 (GRCm39) missense probably damaging 1.00
R2038:Vmn2r54 UTSW 7 12,363,637 (GRCm39) missense possibly damaging 0.94
R2160:Vmn2r54 UTSW 7 12,349,420 (GRCm39) missense probably benign 0.29
R2397:Vmn2r54 UTSW 7 12,349,578 (GRCm39) missense probably damaging 0.98
R2430:Vmn2r54 UTSW 7 12,365,933 (GRCm39) missense probably damaging 0.99
R2829:Vmn2r54 UTSW 7 12,349,617 (GRCm39) missense possibly damaging 0.62
R2975:Vmn2r54 UTSW 7 12,369,919 (GRCm39) missense possibly damaging 0.92
R3005:Vmn2r54 UTSW 7 12,349,221 (GRCm39) missense probably benign 0.28
R4486:Vmn2r54 UTSW 7 12,366,199 (GRCm39) nonsense probably null
R4881:Vmn2r54 UTSW 7 12,363,598 (GRCm39) missense probably benign 0.00
R4907:Vmn2r54 UTSW 7 12,350,150 (GRCm39) splice site probably null
R5536:Vmn2r54 UTSW 7 12,366,343 (GRCm39) missense probably benign 0.03
R5637:Vmn2r54 UTSW 7 12,349,296 (GRCm39) missense probably benign 0.41
R5703:Vmn2r54 UTSW 7 12,363,594 (GRCm39) missense probably benign 0.22
R5769:Vmn2r54 UTSW 7 12,349,209 (GRCm39) missense possibly damaging 0.73
R5972:Vmn2r54 UTSW 7 12,369,874 (GRCm39) missense probably damaging 1.00
R5972:Vmn2r54 UTSW 7 12,349,279 (GRCm39) missense probably damaging 1.00
R5977:Vmn2r54 UTSW 7 12,366,143 (GRCm39) missense probably damaging 1.00
R6084:Vmn2r54 UTSW 7 12,366,205 (GRCm39) missense probably damaging 0.98
R6176:Vmn2r54 UTSW 7 12,349,908 (GRCm39) missense probably damaging 1.00
R6229:Vmn2r54 UTSW 7 12,365,883 (GRCm39) missense probably benign 0.00
R6371:Vmn2r54 UTSW 7 12,349,362 (GRCm39) missense probably damaging 1.00
R6374:Vmn2r54 UTSW 7 12,349,420 (GRCm39) missense probably damaging 1.00
R6804:Vmn2r54 UTSW 7 12,363,792 (GRCm39) missense probably benign
R6886:Vmn2r54 UTSW 7 12,366,080 (GRCm39) missense probably benign 0.02
R7041:Vmn2r54 UTSW 7 12,363,751 (GRCm39) missense probably damaging 0.99
R7058:Vmn2r54 UTSW 7 12,349,722 (GRCm39) missense possibly damaging 0.70
R7113:Vmn2r54 UTSW 7 12,350,001 (GRCm39) missense probably damaging 1.00
R7124:Vmn2r54 UTSW 7 12,356,078 (GRCm39) missense probably benign 0.00
R7126:Vmn2r54 UTSW 7 12,366,088 (GRCm39) missense possibly damaging 0.91
R7236:Vmn2r54 UTSW 7 12,365,917 (GRCm39) missense possibly damaging 0.84
R7337:Vmn2r54 UTSW 7 12,356,044 (GRCm39) missense probably benign 0.00
R7406:Vmn2r54 UTSW 7 12,350,150 (GRCm39) splice site probably null
R7634:Vmn2r54 UTSW 7 12,349,630 (GRCm39) missense probably damaging 1.00
R7793:Vmn2r54 UTSW 7 12,366,196 (GRCm39) missense probably damaging 0.98
R8139:Vmn2r54 UTSW 7 12,349,743 (GRCm39) missense possibly damaging 0.92
R8158:Vmn2r54 UTSW 7 12,349,888 (GRCm39) missense probably damaging 1.00
R8179:Vmn2r54 UTSW 7 12,366,018 (GRCm39) nonsense probably null
R8440:Vmn2r54 UTSW 7 12,350,013 (GRCm39) missense possibly damaging 0.72
R8712:Vmn2r54 UTSW 7 12,369,877 (GRCm39) missense probably benign 0.22
R8853:Vmn2r54 UTSW 7 12,349,782 (GRCm39) missense probably damaging 1.00
R8859:Vmn2r54 UTSW 7 12,363,702 (GRCm39) missense possibly damaging 0.70
R9146:Vmn2r54 UTSW 7 12,366,647 (GRCm39) missense probably benign 0.05
R9157:Vmn2r54 UTSW 7 12,366,055 (GRCm39) missense possibly damaging 0.93
R9344:Vmn2r54 UTSW 7 12,366,283 (GRCm39) missense probably benign
R9423:Vmn2r54 UTSW 7 12,349,441 (GRCm39) missense probably damaging 1.00
R9534:Vmn2r54 UTSW 7 12,366,093 (GRCm39) missense probably benign 0.03
R9632:Vmn2r54 UTSW 7 12,363,753 (GRCm39) missense possibly damaging 0.74
R9661:Vmn2r54 UTSW 7 12,349,166 (GRCm39) missense probably benign
R9710:Vmn2r54 UTSW 7 12,363,753 (GRCm39) missense possibly damaging 0.74
U24488:Vmn2r54 UTSW 7 12,349,356 (GRCm39) missense possibly damaging 0.84
X0066:Vmn2r54 UTSW 7 12,349,297 (GRCm39) missense probably damaging 1.00
Z1177:Vmn2r54 UTSW 7 12,366,035 (GRCm39) nonsense probably null
Predicted Primers PCR Primer
(F):5'- CTCATTTCCTGTGCAGAACTG -3'
(R):5'- TCCTATCAGTCCTTGGGGAAG -3'

Sequencing Primer
(F):5'- TGCAGAACTGGACACCCTCTG -3'
(R):5'- CTATCAGTCCTTGGGGAAGATTGAAG -3'
Posted On 2015-03-18