Mutagenetix > Incidental Mutation

Incidental Mutation 'R3729:Mslnl'

270900

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

040719-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3729 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	A	G	5: 114,207,348 (GRCm38)	D954G	probably damaging	Het
Acvr1	T	C	2: 58,462,913 (GRCm38)	K345R	probably null	Het
Aldoart2	A	G	12: 55,566,319 (GRCm38)	Y343C	probably damaging	Het
Arpp21	T	A	9: 112,065,979 (GRCm38)	Y739F	possibly damaging	Het
Cachd1	C	A	4: 100,974,880 (GRCm38)	Y746*	probably null	Het
Card14	T	C	11: 119,333,932 (GRCm38)	V595A	probably damaging	Het
Dhx33	T	C	11: 70,989,152 (GRCm38)	D344G	probably benign	Het
Dnah12	T	A	14: 26,706,065 (GRCm38)	D171E	probably benign	Het
Fat3	A	G	9: 16,247,041 (GRCm38)		probably benign	Het
Fmnl3	A	G	15: 99,321,864 (GRCm38)	F668L	probably damaging	Het
Frmpd4	G	A	X: 167,486,807 (GRCm38)	T493M	probably damaging	Het
Gcn1l1	A	T	5: 115,583,394 (GRCm38)		probably benign	Het
Hspbp1	G	T	7: 4,677,809 (GRCm38)	Q223K	probably damaging	Het
Ighv1-19	C	A	12: 114,708,877 (GRCm38)	C40F	probably damaging	Het
Igkv4-90	A	G	6: 68,807,681 (GRCm38)	F10L	probably benign	Het
Kctd5	T	C	17: 24,059,238 (GRCm38)	D146G	probably benign	Het
Kif4-ps	A	G	12: 101,146,110 (GRCm38)	E166G	probably damaging	Het
Lemd3	T	C	10: 120,928,015 (GRCm38)	D780G	probably damaging	Het
Manba	G	A	3: 135,554,850 (GRCm38)	V599I	probably benign	Het
Map2	A	G	1: 66,412,446 (GRCm38)	E247G	possibly damaging	Het
Mfsd1	T	C	3: 67,582,965 (GRCm38)	F50L	probably benign	Het
Mier2	T	C	10: 79,545,042 (GRCm38)		probably benign	Het
Nol10	A	T	12: 17,424,673 (GRCm38)	K622I	probably benign	Het
Olfr486	A	T	7: 108,172,309 (GRCm38)	I145N	probably benign	Het
Olfr926	T	C	9: 38,877,251 (GRCm38)	F25S	probably damaging	Het
Phtf1	A	G	3: 103,985,779 (GRCm38)	M120V	probably benign	Het
Prrx1	A	G	1: 163,261,877 (GRCm38)	L127P	probably damaging	Het
Rbsn	T	C	6: 92,191,335 (GRCm38)	M373V	possibly damaging	Het
Sec24b	T	C	3: 130,033,833 (GRCm38)	K203R	possibly damaging	Het
Shroom3	G	T	5: 92,943,086 (GRCm38)	V1151F	probably damaging	Het
Slc16a10	G	C	10: 40,056,624 (GRCm38)	H314D	possibly damaging	Het
Slc4a7	A	T	14: 14,729,276 (GRCm38)	E47D	probably damaging	Het
Taf12	C	A	4: 132,282,954 (GRCm38)	P78Q	probably damaging	Het
Tnn	A	G	1: 160,146,240 (GRCm38)	C186R	probably damaging	Het
Trim46	T	A	3: 89,234,949 (GRCm38)	T721S	probably benign	Het
Trip4	T	C	9: 65,880,942 (GRCm38)	N74D	possibly damaging	Het
Tssk5	C	T	15: 76,372,896 (GRCm38)	R262H	probably benign	Het
Vegfa	T	C	17: 46,024,520 (GRCm38)	I105V	possibly damaging	Het
Vmn2r14	T	C	5: 109,216,229 (GRCm38)	Y607C	probably damaging	Het
Wdr83	G	T	8: 85,080,339 (GRCm38)	H66Q	probably damaging	Het
Zfp280b	A	G	10: 76,039,102 (GRCm38)	T272A	probably benign	Het
Zfp960	T	A	17: 17,088,371 (GRCm38)	L449H	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,743,667 (GRCm38)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,744,775 (GRCm38)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,746,151 (GRCm38)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,747,998 (GRCm38)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,744,103 (GRCm38)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,744,077 (GRCm38)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,743,203 (GRCm38)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,742,965 (GRCm38)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,744,517 (GRCm38)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,744,969 (GRCm38)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,738,978 (GRCm38)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,738,968 (GRCm38)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,746,165 (GRCm38)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,743,159 (GRCm38)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,737,842 (GRCm38)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,746,775 (GRCm38)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,737,902 (GRCm38)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,744,557 (GRCm38)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,746,073 (GRCm38)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,743,212 (GRCm38)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,743,210 (GRCm38)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,736,921 (GRCm38)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,743,183 (GRCm38)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,746,777 (GRCm38)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,746,988 (GRCm38)	missense	probably benign
R8735:Mslnl	UTSW	17	25,745,088 (GRCm38)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,745,073 (GRCm38)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,742,720 (GRCm38)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,742,532 (GRCm38)	intron	probably benign
RF007:Mslnl	UTSW	17	25,743,228 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CTGCCATCAGAAGGGAGGTTTG -3'
(R):5'- CCAAGAAGCCACAGGTCTGAAG -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- CCACAGGTCTGAAGCACTGAG -3'

Posted On

2015-03-18