Mutagenetix > Incidental Mutation

Incidental Mutation 'R3730:Mslnl'

270950

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3730 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.2%
20x: 90.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	A	G	1: 11,545,226 (GRCm38)	N141S	probably damaging	Het
Acbd6	T	A	1: 155,558,725 (GRCm38)	S30T	probably benign	Het
Acot12	T	A	13: 91,760,026 (GRCm38)	F109Y	possibly damaging	Het
Adamts19	G	A	18: 58,900,910 (GRCm38)	R319Q	probably damaging	Het
Akap1	T	C	11: 88,845,182 (GRCm38)	E218G	possibly damaging	Het
Atg4b	T	A	1: 93,768,275 (GRCm38)	D45E	probably damaging	Het
Atoh1	A	G	6: 64,729,573 (GRCm38)	E84G	probably benign	Het
Cfap54	A	T	10: 93,011,473 (GRCm38)	Y951*	probably null	Het
Col4a4	T	A	1: 82,455,751 (GRCm38)		probably null	Het
Crlf1	A	G	8: 70,499,442 (GRCm38)	T95A	probably benign	Het
Cyp3a25	G	A	5: 146,003,081 (GRCm38)	P39S	probably damaging	Het
Dhx9	C	A	1: 153,478,120 (GRCm38)	A186S	probably benign	Het
Dusp16	A	G	6: 134,718,861 (GRCm38)	S336P	probably benign	Het
Fcgbp	T	C	7: 28,085,457 (GRCm38)	V314A	possibly damaging	Het
Focad	T	C	4: 88,408,925 (GRCm38)	I157T	possibly damaging	Het
Frem3	A	T	8: 80,615,916 (GRCm38)	T1613S	probably damaging	Het
Fshr	C	T	17: 89,001,715 (GRCm38)	V222I	probably benign	Het
Galnt13	A	G	2: 54,933,507 (GRCm38)	N365S	possibly damaging	Het
Hjurp	GT	GTT	1: 88,266,524 (GRCm38)		probably null	Het
Ice1	A	T	13: 70,603,240 (GRCm38)	S1576T	probably damaging	Het
Ighv1-19	C	A	12: 114,708,877 (GRCm38)	C40F	probably damaging	Het
Itga8	T	C	2: 12,193,510 (GRCm38)	T555A	possibly damaging	Het
Kctd5	T	C	17: 24,059,238 (GRCm38)	D146G	probably benign	Het
Ktn1	A	G	14: 47,701,149 (GRCm38)	E766G	probably damaging	Het
Ldlr	C	G	9: 21,731,801 (GRCm38)	A41G	probably benign	Het
Lrp2	G	A	2: 69,464,579 (GRCm38)	P3465L	probably damaging	Het
Lrp2	A	T	2: 69,534,907 (GRCm38)		probably null	Het
Mapk11	G	A	15: 89,145,115 (GRCm38)	A248V	probably benign	Het
Mroh2a	GCCC	GC	1: 88,232,257 (GRCm38)		probably null	Het
Npr2	T	C	4: 43,640,999 (GRCm38)	S402P	possibly damaging	Het
Olfm2	T	C	9: 20,672,767 (GRCm38)	N76D	probably damaging	Het
Olfr1047	A	G	2: 86,228,851 (GRCm38)	I40T	probably benign	Het
Olfr853	T	A	9: 19,537,151 (GRCm38)	I260F	probably benign	Het
Pias4	A	T	10: 81,164,054 (GRCm38)	F55Y	probably damaging	Het
Rgs12	G	A	5: 35,032,251 (GRCm38)	E658K	probably damaging	Het
Ripor3	C	G	2: 167,992,819 (GRCm38)	E251Q	probably damaging	Het
Shroom3	G	T	5: 92,943,086 (GRCm38)	V1151F	probably damaging	Het
Slc16a10	G	C	10: 40,056,624 (GRCm38)	H314D	possibly damaging	Het
Slc35e4	A	G	11: 3,912,577 (GRCm38)	V204A	possibly damaging	Het
Syt4	T	C	18: 31,444,136 (GRCm38)	H55R	probably damaging	Het
Tmem2	A	G	19: 21,826,117 (GRCm38)	Y838C	probably damaging	Het
Trim46	T	A	3: 89,234,949 (GRCm38)	T721S	probably benign	Het
Usf3	G	T	16: 44,218,575 (GRCm38)	L1139F	probably benign	Het
Wdr66	A	T	5: 123,326,568 (GRCm38)	I1280L	possibly damaging	Het
Xrn2	A	T	2: 147,024,809 (GRCm38)	M100L	probably benign	Het
Zbtb33	C	A	X: 38,192,945 (GRCm38)	N243K	probably benign	Het
Zfp960	T	A	17: 17,088,371 (GRCm38)	L449H	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,743,667 (GRCm38)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,744,775 (GRCm38)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,746,151 (GRCm38)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,747,998 (GRCm38)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,744,103 (GRCm38)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,744,077 (GRCm38)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,743,203 (GRCm38)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,742,965 (GRCm38)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,744,517 (GRCm38)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,744,969 (GRCm38)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,738,978 (GRCm38)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,738,968 (GRCm38)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,746,165 (GRCm38)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,743,159 (GRCm38)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,737,842 (GRCm38)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,746,775 (GRCm38)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,737,902 (GRCm38)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,744,557 (GRCm38)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,746,073 (GRCm38)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,743,212 (GRCm38)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,743,210 (GRCm38)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,736,921 (GRCm38)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,743,183 (GRCm38)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,746,777 (GRCm38)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,746,988 (GRCm38)	missense	probably benign
R8735:Mslnl	UTSW	17	25,745,088 (GRCm38)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,745,073 (GRCm38)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,742,720 (GRCm38)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,742,532 (GRCm38)	intron	probably benign
RF007:Mslnl	UTSW	17	25,743,228 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GCCATCAGAAGGGAGGTTTG -3'
(R):5'- ACAGGTCTGAAGCACTGAGC -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2015-03-18