Mutagenetix > Incidental Mutation

Incidental Mutation 'R3745:Acot9'

271191

Institutional Source

Beutler Lab

Gene Symbol

Acot9

Ensembl Gene

ENSMUSG00000025287

Gene Name

acyl-CoA thioesterase 9

Synonyms

p48, MT-ACT48, Acate2, 0610041P13Rik, U8

MMRRC Submission

040731-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.179) question?

Stock #

R3745 (G1)

Quality Score

222

Status

Validated

Chromosome

Chromosomal Location

154045439-154080650 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

G to A at 154054941 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000138519 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026324] [ENSMUST00000131332]

AlphaFold

Q9R0X4

Predicted Effect

probably benign
Transcript: ENSMUST00000026324

SMART Domains

Protein: ENSMUSP00000026324
Gene: ENSMUSG00000025287

Domain	Start	End	E-Value	Type
SCOP:d1lo7a_	108	222	1e-3	SMART
PDB:4IEN\|D	277	400	8e-7	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000131332

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	G	T	11: 109,684,980 (GRCm39)	C172*	probably null	Het
Akap10	A	G	11: 61,806,131 (GRCm39)	V199A	probably benign	Het
Aox4	C	T	1: 58,285,029 (GRCm39)	H594Y	probably damaging	Het
Arhgap35	A	T	7: 16,297,647 (GRCm39)	Y473N	probably damaging	Het
Aspn	G	A	13: 49,720,036 (GRCm39)	E351K	probably damaging	Het
Astn1	G	T	1: 158,329,630 (GRCm39)	A162S	probably damaging	Het
Atosa	T	C	9: 74,917,144 (GRCm39)	V581A	probably benign	Het
Auts2	A	G	5: 131,505,425 (GRCm39)		probably benign	Het
Cog6	A	T	3: 52,900,240 (GRCm39)	M507K	probably benign	Het
Crct1	C	A	3: 92,922,014 (GRCm39)		probably benign	Het
Cyp2d11	A	C	15: 82,276,056 (GRCm39)	I175S	probably benign	Het
Dclk1	A	G	3: 55,154,863 (GRCm39)	N98D	possibly damaging	Het
Erich5	T	A	15: 34,470,878 (GRCm39)	C36S	probably damaging	Het
F5	A	G	1: 164,014,348 (GRCm39)	I540V	possibly damaging	Het
Fam20a	A	T	11: 109,568,616 (GRCm39)	S303R	probably benign	Het
Fat3	G	A	9: 15,909,567 (GRCm39)	S2145F	probably damaging	Het
Gbp9	C	A	5: 105,253,724 (GRCm39)		probably benign	Het
Gm6408	G	T	5: 146,421,246 (GRCm39)	V292F	probably damaging	Het
Kmt2a	A	G	9: 44,742,637 (GRCm39)		probably benign	Het
Lrriq1	T	C	10: 103,006,717 (GRCm39)	D1136G	probably damaging	Het
Macrod2	C	A	2: 141,652,549 (GRCm39)	T204K	probably damaging	Het
Mlkl	A	G	8: 112,042,199 (GRCm39)		probably benign	Het
Msantd1	T	A	5: 35,080,811 (GRCm39)	V155E	possibly damaging	Het
Myo3b	A	G	2: 70,064,829 (GRCm39)		probably benign	Het
Nbn	T	A	4: 15,976,163 (GRCm39)	C375S	possibly damaging	Het
Nell2	A	C	15: 95,330,554 (GRCm39)	C231W	probably damaging	Het
Nipbl	A	G	15: 8,388,358 (GRCm39)	S421P	probably benign	Het
Npr3	A	T	15: 11,905,577 (GRCm39)	V50E	probably damaging	Het
Or4d10	A	G	19: 12,051,744 (GRCm39)	L84P	probably damaging	Het
Pclo	T	C	5: 14,728,435 (GRCm39)		probably benign	Het
Pkn3	A	G	2: 29,980,353 (GRCm39)	K785R	probably damaging	Het
Ppef2	T	A	5: 92,387,010 (GRCm39)		probably benign	Het
Prdm10	T	C	9: 31,251,703 (GRCm39)	I357T	possibly damaging	Het
Prrc2c	G	A	1: 162,525,754 (GRCm39)	T284I	unknown	Het
Psma3	T	C	12: 71,025,522 (GRCm39)	S13P	possibly damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Rpl6l	T	C	10: 110,962,226 (GRCm39)		noncoding transcript	Het
Tex11	A	G	X: 99,960,178 (GRCm39)	V522A	probably benign	Het
Thsd7b	A	G	1: 129,605,978 (GRCm39)	E573G	probably benign	Het
Tom1l1	A	G	11: 90,548,567 (GRCm39)	S259P	probably benign	Het
Trpm8	A	G	1: 88,276,049 (GRCm39)	E549G	probably benign	Het
Vmn1r66	C	T	7: 10,008,248 (GRCm39)	A262T	possibly damaging	Het
Zc3h13	T	A	14: 75,568,101 (GRCm39)	D1131E	probably benign	Het
Zfp445	A	C	9: 122,683,791 (GRCm39)	D289E	probably benign	Het

Other mutations in Acot9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00589:Acot9	APN	X	154,078,236 (GRCm39)	missense	possibly damaging	0.50
IGL00987:Acot9	APN	X	154,078,177 (GRCm39)	missense	probably benign	0.00
IGL03218:Acot9	APN	X	154,078,207 (GRCm39)	missense	possibly damaging	0.48
R4498:Acot9	UTSW	X	154,047,064 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATTTGCACTGAGGAAGCTGC -3'
(R):5'- CTGATCACTACACTAAGACGCG -3'

Sequencing Primer
(F):5'- TTGCACTGAGGAAGCTGCATAATTG -3'
(R):5'- ACACTAAGACGCGGCTCTTTTC -3'

Posted On

2015-03-18