Mutagenetix > Incidental Mutation

Incidental Mutation 'R3752:Lama3'

271282

Institutional Source

Beutler Lab

Gene Symbol

Lama3

Ensembl Gene

ENSMUSG00000024421

Gene Name

laminin, alpha 3

Synonyms

[a]3B, nicein, 150kDa

MMRRC Submission

040737-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R3752 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12466876-12716070 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 12640086 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 1700 (C1700S)

Ref Sequence

ENSEMBL: ENSMUSP00000089703 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092070] [ENSMUST00000188815]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000092070
AA Change: C1700S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000089703
Gene: ENSMUSG00000024421
AA Change: C1700S

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
LamNT	38	294	1.46e-153	SMART
EGF_Lam	296	350	1.39e-4	SMART
EGF_Lam	353	420	2.66e-10	SMART
EGF_Lam	423	464	3.51e-10	SMART
EGF_Lam	488	530	1.73e-9	SMART
EGF_Lam	533	576	3.81e-11	SMART
EGF_like	579	625	1.82e-1	SMART
EGF_Lam	628	678	5.15e-8	SMART
EGF_Lam	681	725	3.54e-6	SMART
low complexity region	768	781	N/A	INTRINSIC
EGF_Lam	1263	1306	3.15e-12	SMART
EGF_Lam	1309	1350	6.3e-3	SMART
EGF_Lam	1353	1399	1.49e-13	SMART
EGF_Lam	1402	1450	8.18e-11	SMART
LamB	1509	1638	4.34e-55	SMART
Pfam:Laminin_EGF	1647	1681	7.9e-5	PFAM
EGF_Lam	1684	1728	2.66e-10	SMART
EGF_Lam	1731	1781	7.81e-8	SMART
Pfam:Laminin_I	1836	2102	2.7e-93	PFAM
low complexity region	2185	2200	N/A	INTRINSIC
coiled coil region	2211	2238	N/A	INTRINSIC
LamG	2406	2566	1.67e-2	SMART
LamG	2614	2742	1.72e-17	SMART
LamG	2785	2900	3.96e-17	SMART
LamG	3005	3133	1.12e-34	SMART
LamG	3175	3308	3.41e-30	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000188118

Predicted Effect

possibly damaging
Transcript: ENSMUST00000188815
AA Change: C94S

PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000140104
Gene: ENSMUSG00000024421
AA Change: C94S

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_Lam	78	122	2.66e-10	SMART
EGF_Lam	125	175	7.81e-8	SMART
Pfam:Laminin_I	230	496	1e-90	PFAM
low complexity region	579	594	N/A	INTRINSIC
coiled coil region	605	632	N/A	INTRINSIC
LamG	800	960	1.67e-2	SMART
LamG	1008	1136	1.72e-17	SMART
LamG	1179	1294	3.96e-17	SMART
LamG	1399	1527	1.12e-34	SMART
LamG	1569	1702	3.41e-30	SMART

Meta Mutation Damage Score

0.8575

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

100% (49/49)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	T	A	1: 11,588,904 (GRCm39)	I107N	probably damaging	Het
Adh1	G	A	3: 137,994,555 (GRCm39)	V292I	probably benign	Het
Brd1	C	T	15: 88,573,821 (GRCm39)	V1093I	possibly damaging	Het
Cep170	A	G	1: 176,610,061 (GRCm39)		probably benign	Het
Col4a4	G	A	1: 82,458,215 (GRCm39)	P1120S	probably damaging	Het
Cramp1	A	T	17: 25,190,532 (GRCm39)	N1067K	probably damaging	Het
Dhrs7c	A	G	11: 67,702,281 (GRCm39)	T90A	probably damaging	Het
E2f1	C	G	2: 154,405,942 (GRCm39)	G144R	probably damaging	Het
Eml6	C	A	11: 29,759,360 (GRCm39)	V798F	probably benign	Het
Ext1	A	T	15: 52,939,306 (GRCm39)	V581E	probably damaging	Het
Fbf1	C	T	11: 116,038,622 (GRCm39)	R833Q	probably benign	Het
Fbln1	T	A	15: 85,111,279 (GRCm39)	C144*	probably null	Het
Gm10521	A	G	1: 171,723,712 (GRCm39)	T8A	unknown	Het
Hapln4	A	T	8: 70,539,615 (GRCm39)	L215F	probably damaging	Het
Hmgcr	T	C	13: 96,799,624 (GRCm39)	I157V	probably damaging	Het
Irak4	T	C	15: 94,459,476 (GRCm39)	I364T	probably damaging	Het
Lama5	C	T	2: 179,829,015 (GRCm39)	C2042Y	probably damaging	Het
Lrrc9	T	G	12: 72,507,580 (GRCm39)	Y360*	probably null	Het
Lrrd1	T	A	5: 3,900,282 (GRCm39)	S196T	probably benign	Het
Mdc1	C	T	17: 36,156,821 (GRCm39)	A76V	probably damaging	Het
Mei1	C	T	15: 81,970,383 (GRCm39)	T428M	probably damaging	Het
Mib2	A	G	4: 155,739,741 (GRCm39)	F810S	probably damaging	Het
Nobox	T	G	6: 43,284,167 (GRCm39)	K126N	probably damaging	Het
Ogn	C	T	13: 49,776,307 (GRCm39)	L249F	probably benign	Het
Or10ag59	A	G	2: 87,406,057 (GRCm39)	I210V	probably benign	Het
Or6a2	A	T	7: 106,600,682 (GRCm39)	Y128*	probably null	Het
Paqr8	A	G	1: 21,005,856 (GRCm39)	T337A	probably benign	Het
Pcdhb15	T	A	18: 37,606,810 (GRCm39)	V14E	probably damaging	Het
Perm1	A	T	4: 156,302,403 (GRCm39)	I316L	probably benign	Het
Plxnb2	G	A	15: 89,041,458 (GRCm39)		probably benign	Het
Rab3il1	A	T	19: 10,007,841 (GRCm39)	T227S	probably benign	Het
Rag2	A	T	2: 101,461,121 (GRCm39)	Y477F	probably damaging	Het
Ralgapa2	A	T	2: 146,263,551 (GRCm39)	V722E	possibly damaging	Het
Rbp3	A	T	14: 33,677,969 (GRCm39)	E639V	probably damaging	Het
Sh2b1	A	G	7: 126,067,959 (GRCm39)	V565A	probably damaging	Het
Skint6	T	C	4: 112,700,096 (GRCm39)		probably benign	Het
Slc47a1	C	T	11: 61,235,207 (GRCm39)	R542Q	possibly damaging	Het
Slc6a5	T	C	7: 49,586,062 (GRCm39)		probably null	Het
Slc9a8	C	A	2: 167,299,272 (GRCm39)	H215N	probably benign	Het
Slit1	C	T	19: 41,635,406 (GRCm39)		probably null	Het
Snx1	A	T	9: 66,012,933 (GRCm39)		probably null	Het
Tbc1d30	T	A	10: 121,108,073 (GRCm39)	N443I	probably damaging	Het
Tex15	T	A	8: 34,061,443 (GRCm39)	M565K	probably benign	Het
Traf3ip1	T	C	1: 91,428,639 (GRCm39)		probably benign	Het
Traf3ip1	A	G	1: 91,446,019 (GRCm39)	D384G	probably damaging	Het
Vldlr	G	A	19: 27,215,731 (GRCm39)	E243K	probably damaging	Het
Vps35	A	G	8: 86,001,460 (GRCm39)	S453P	probably benign	Het
Zfp644	A	G	5: 106,784,249 (GRCm39)	V766A	probably benign	Het

Other mutations in Lama3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Lama3	APN	18	12,713,349 (GRCm39)	missense	probably benign
IGL00272:Lama3	APN	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
IGL00335:Lama3	APN	18	12,582,645 (GRCm39)	splice site	probably benign
IGL00836:Lama3	APN	18	12,605,285 (GRCm39)	missense	probably benign	0.01
IGL01017:Lama3	APN	18	12,574,200 (GRCm39)	critical splice donor site	probably null
IGL01025:Lama3	APN	18	12,614,094 (GRCm39)	missense	probably benign	0.09
IGL01394:Lama3	APN	18	12,664,983 (GRCm39)	missense	probably null	0.39
IGL01545:Lama3	APN	18	12,574,188 (GRCm39)	missense	probably benign	0.01
IGL01685:Lama3	APN	18	12,586,937 (GRCm39)	splice site	probably benign
IGL01863:Lama3	APN	18	12,552,993 (GRCm39)	splice site	probably benign
IGL01869:Lama3	APN	18	12,657,820 (GRCm39)	missense	possibly damaging	0.94
IGL01894:Lama3	APN	18	12,705,121 (GRCm39)	missense	probably benign	0.09
IGL02027:Lama3	APN	18	12,649,570 (GRCm39)	missense	probably damaging	1.00
IGL02106:Lama3	APN	18	12,601,371 (GRCm39)	missense	probably damaging	0.98
IGL02307:Lama3	APN	18	12,714,840 (GRCm39)	missense	probably benign	0.09
IGL02342:Lama3	APN	18	12,624,533 (GRCm39)	missense	probably damaging	1.00
IGL02377:Lama3	APN	18	12,689,807 (GRCm39)	missense	possibly damaging	0.49
IGL02401:Lama3	APN	18	12,690,784 (GRCm39)	missense	probably benign	0.02
IGL02517:Lama3	APN	18	12,670,915 (GRCm39)	critical splice donor site	probably null
IGL02644:Lama3	APN	18	12,658,910 (GRCm39)	missense	probably benign	0.12
IGL02733:Lama3	APN	18	12,711,184 (GRCm39)	missense	probably damaging	0.99
IGL02932:Lama3	APN	18	12,661,858 (GRCm39)	missense	probably damaging	1.00
IGL03006:Lama3	APN	18	12,601,425 (GRCm39)	splice site	probably benign
IGL03038:Lama3	APN	18	12,552,307 (GRCm39)	missense	probably damaging	0.99
IGL03064:Lama3	APN	18	12,572,406 (GRCm39)	missense	possibly damaging	0.72
IGL03146:Lama3	APN	18	12,660,681 (GRCm39)	missense	possibly damaging	0.66
IGL03233:Lama3	APN	18	12,614,095 (GRCm39)	missense	probably damaging	1.00
IGL03255:Lama3	APN	18	12,672,760 (GRCm39)	missense	probably damaging	1.00
IGL03369:Lama3	APN	18	12,686,340 (GRCm39)	missense	probably benign	0.05
IGL03412:Lama3	APN	18	12,552,239 (GRCm39)	missense	probably damaging	0.99
IGL02980:Lama3	UTSW	18	12,686,288 (GRCm39)	missense	probably benign	0.01
IGL03014:Lama3	UTSW	18	12,673,024 (GRCm39)	missense	possibly damaging	0.95
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0106:Lama3	UTSW	18	12,537,039 (GRCm39)	missense	probably damaging	0.96
R0148:Lama3	UTSW	18	12,581,329 (GRCm39)	missense	probably damaging	1.00
R0165:Lama3	UTSW	18	12,657,867 (GRCm39)	missense	probably damaging	0.99
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0316:Lama3	UTSW	18	12,652,934 (GRCm39)	missense	probably benign	0.09
R0325:Lama3	UTSW	18	12,615,183 (GRCm39)	missense	probably damaging	1.00
R0365:Lama3	UTSW	18	12,640,064 (GRCm39)	missense	probably damaging	0.96
R0390:Lama3	UTSW	18	12,540,620 (GRCm39)	missense	probably benign	0.10
R0408:Lama3	UTSW	18	12,589,894 (GRCm39)	missense	probably benign
R0449:Lama3	UTSW	18	12,633,569 (GRCm39)	splice site	probably null
R0453:Lama3	UTSW	18	12,598,535 (GRCm39)	missense	possibly damaging	0.63
R0480:Lama3	UTSW	18	12,583,481 (GRCm39)	missense	possibly damaging	0.81
R0536:Lama3	UTSW	18	12,658,951 (GRCm39)	missense	probably damaging	1.00
R0545:Lama3	UTSW	18	12,694,758 (GRCm39)	missense	possibly damaging	0.90
R0567:Lama3	UTSW	18	12,682,309 (GRCm39)	missense	probably benign
R0605:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R0617:Lama3	UTSW	18	12,552,315 (GRCm39)	critical splice donor site	probably null
R0629:Lama3	UTSW	18	12,552,302 (GRCm39)	missense	possibly damaging	0.79
R0671:Lama3	UTSW	18	12,610,647 (GRCm39)	missense	possibly damaging	0.80
R0730:Lama3	UTSW	18	12,589,907 (GRCm39)	splice site	probably benign
R1216:Lama3	UTSW	18	12,554,191 (GRCm39)	splice site	probably benign
R1356:Lama3	UTSW	18	12,633,634 (GRCm39)	unclassified	probably benign
R1386:Lama3	UTSW	18	12,610,427 (GRCm39)	missense	probably benign	0.04
R1424:Lama3	UTSW	18	12,653,048 (GRCm39)	missense	probably benign	0.13
R1426:Lama3	UTSW	18	12,614,155 (GRCm39)	critical splice donor site	probably null
R1437:Lama3	UTSW	18	12,682,284 (GRCm39)	missense	possibly damaging	0.46
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1472:Lama3	UTSW	18	12,615,102 (GRCm39)	missense	probably benign	0.23
R1557:Lama3	UTSW	18	12,646,788 (GRCm39)	splice site	probably benign
R1571:Lama3	UTSW	18	12,672,774 (GRCm39)	missense	probably damaging	0.98
R1599:Lama3	UTSW	18	12,583,457 (GRCm39)	nonsense	probably null
R1631:Lama3	UTSW	18	12,540,551 (GRCm39)	missense	probably damaging	1.00
R1647:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1648:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1719:Lama3	UTSW	18	12,612,929 (GRCm39)	critical splice donor site	probably null
R1757:Lama3	UTSW	18	12,598,556 (GRCm39)	missense	probably benign	0.10
R1766:Lama3	UTSW	18	12,535,119 (GRCm39)	missense	probably damaging	1.00
R1853:Lama3	UTSW	18	12,646,762 (GRCm39)	missense	possibly damaging	0.75
R1856:Lama3	UTSW	18	12,670,838 (GRCm39)	nonsense	probably null
R1909:Lama3	UTSW	18	12,714,855 (GRCm39)	missense	probably benign	0.19
R1913:Lama3	UTSW	18	12,628,336 (GRCm39)	missense	probably benign	0.15
R1975:Lama3	UTSW	18	12,586,920 (GRCm39)	missense	probably damaging	1.00
R2014:Lama3	UTSW	18	12,657,778 (GRCm39)	splice site	probably benign
R2059:Lama3	UTSW	18	12,661,390 (GRCm39)	missense	probably damaging	0.98
R2060:Lama3	UTSW	18	12,661,783 (GRCm39)	missense	probably benign	0.30
R2086:Lama3	UTSW	18	12,657,887 (GRCm39)	missense	probably benign	0.39
R2115:Lama3	UTSW	18	12,535,906 (GRCm39)	missense	possibly damaging	0.94
R2291:Lama3	UTSW	18	12,658,136 (GRCm39)	missense	probably damaging	0.98
R2860:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2861:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2862:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R3410:Lama3	UTSW	18	12,546,915 (GRCm39)	critical splice donor site	probably null
R3614:Lama3	UTSW	18	12,581,345 (GRCm39)	missense	probably benign	0.03
R3696:Lama3	UTSW	18	12,572,532 (GRCm39)	splice site	probably benign
R3967:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3968:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3969:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3970:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R4088:Lama3	UTSW	18	12,637,365 (GRCm39)	nonsense	probably null
R4118:Lama3	UTSW	18	12,583,488 (GRCm39)	missense	probably benign	0.01
R4222:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4223:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4224:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4225:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4367:Lama3	UTSW	18	12,646,747 (GRCm39)	missense	probably damaging	1.00
R4404:Lama3	UTSW	18	12,715,588 (GRCm39)	missense	probably benign	0.01
R4424:Lama3	UTSW	18	12,652,929 (GRCm39)	nonsense	probably null
R4483:Lama3	UTSW	18	12,682,310 (GRCm39)	missense	probably benign	0.32
R4484:Lama3	UTSW	18	12,614,145 (GRCm39)	missense	probably benign
R4516:Lama3	UTSW	18	12,628,415 (GRCm39)	missense	probably damaging	1.00
R4556:Lama3	UTSW	18	12,612,816 (GRCm39)	missense	possibly damaging	0.63
R4616:Lama3	UTSW	18	12,637,454 (GRCm39)	critical splice donor site	probably null
R4702:Lama3	UTSW	18	12,711,086 (GRCm39)	nonsense	probably null
R4704:Lama3	UTSW	18	12,686,280 (GRCm39)	missense	probably benign	0.08
R4750:Lama3	UTSW	18	12,637,416 (GRCm39)	missense	probably benign	0.25
R4753:Lama3	UTSW	18	12,615,141 (GRCm39)	missense	probably damaging	1.00
R4767:Lama3	UTSW	18	12,633,620 (GRCm39)	missense	probably benign	0.32
R4777:Lama3	UTSW	18	12,546,828 (GRCm39)	missense	probably damaging	1.00
R4782:Lama3	UTSW	18	12,544,627 (GRCm39)	nonsense	probably null
R4784:Lama3	UTSW	18	12,582,601 (GRCm39)	missense	probably benign	0.20
R4816:Lama3	UTSW	18	12,610,661 (GRCm39)	missense	possibly damaging	0.93
R4833:Lama3	UTSW	18	12,574,188 (GRCm39)	missense	probably benign	0.01
R4854:Lama3	UTSW	18	12,544,599 (GRCm39)	missense	probably benign	0.00
R4863:Lama3	UTSW	18	12,631,735 (GRCm39)	intron	probably benign
R4863:Lama3	UTSW	18	12,672,850 (GRCm39)	missense	probably damaging	0.99
R4953:Lama3	UTSW	18	12,581,362 (GRCm39)	missense	probably damaging	1.00
R4974:Lama3	UTSW	18	12,685,883 (GRCm39)	missense	probably damaging	0.98
R4996:Lama3	UTSW	18	12,651,800 (GRCm39)	missense	probably benign	0.24
R5049:Lama3	UTSW	18	12,715,668 (GRCm39)	missense	probably benign	0.19
R5057:Lama3	UTSW	18	12,665,005 (GRCm39)	missense	probably null	0.82
R5090:Lama3	UTSW	18	12,675,459 (GRCm39)	missense	possibly damaging	0.94
R5122:Lama3	UTSW	18	12,672,823 (GRCm39)	missense	possibly damaging	0.53
R5215:Lama3	UTSW	18	12,710,957 (GRCm39)	missense	probably damaging	1.00
R5245:Lama3	UTSW	18	12,552,950 (GRCm39)	missense	probably damaging	1.00
R5259:Lama3	UTSW	18	12,598,565 (GRCm39)	missense	probably damaging	1.00
R5320:Lama3	UTSW	18	12,685,912 (GRCm39)	missense	probably damaging	0.99
R5377:Lama3	UTSW	18	12,586,803 (GRCm39)	missense	probably damaging	0.99
R5432:Lama3	UTSW	18	12,705,123 (GRCm39)	missense	probably damaging	1.00
R5500:Lama3	UTSW	18	12,589,821 (GRCm39)	missense	possibly damaging	0.93
R5534:Lama3	UTSW	18	12,686,267 (GRCm39)	missense	probably benign	0.00
R5589:Lama3	UTSW	18	12,605,277 (GRCm39)	missense	possibly damaging	0.46
R5604:Lama3	UTSW	18	12,572,405 (GRCm39)	missense	probably benign
R5617:Lama3	UTSW	18	12,631,993 (GRCm39)	intron	probably benign
R5709:Lama3	UTSW	18	12,672,856 (GRCm39)	missense	probably damaging	1.00
R5965:Lama3	UTSW	18	12,562,944 (GRCm39)	missense	possibly damaging	0.67
R6042:Lama3	UTSW	18	12,707,311 (GRCm39)	missense	probably damaging	1.00
R6065:Lama3	UTSW	18	12,602,985 (GRCm39)	missense	possibly damaging	0.53
R6085:Lama3	UTSW	18	12,615,156 (GRCm39)	missense	probably benign	0.01
R6212:Lama3	UTSW	18	12,646,702 (GRCm39)	missense	probably damaging	1.00
R6268:Lama3	UTSW	18	12,657,794 (GRCm39)	missense	probably damaging	0.98
R6276:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R6366:Lama3	UTSW	18	12,615,194 (GRCm39)	missense	probably damaging	1.00
R6393:Lama3	UTSW	18	12,612,813 (GRCm39)	missense	probably benign	0.44
R6493:Lama3	UTSW	18	12,615,205 (GRCm39)	critical splice donor site	probably null
R6505:Lama3	UTSW	18	12,628,405 (GRCm39)	missense	probably benign	0.02
R6563:Lama3	UTSW	18	12,670,823 (GRCm39)	missense	probably damaging	1.00
R6582:Lama3	UTSW	18	12,710,897 (GRCm39)	missense	probably damaging	1.00
R6585:Lama3	UTSW	18	12,552,314 (GRCm39)	critical splice donor site	probably null
R6609:Lama3	UTSW	18	12,646,735 (GRCm39)	missense	probably damaging	0.99
R6656:Lama3	UTSW	18	12,682,283 (GRCm39)	missense	possibly damaging	0.66
R6833:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R6834:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R7019:Lama3	UTSW	18	12,661,475 (GRCm39)	missense	probably damaging	0.97
R7026:Lama3	UTSW	18	12,649,605 (GRCm39)	missense	probably damaging	0.98
R7088:Lama3	UTSW	18	12,715,602 (GRCm39)	missense	possibly damaging	0.90
R7100:Lama3	UTSW	18	12,715,701 (GRCm39)	missense	possibly damaging	0.80
R7102:Lama3	UTSW	18	12,685,870 (GRCm39)	missense	possibly damaging	0.66
R7103:Lama3	UTSW	18	12,664,936 (GRCm39)	missense	probably benign	0.00
R7121:Lama3	UTSW	18	12,595,839 (GRCm39)	missense	probably benign	0.06
R7133:Lama3	UTSW	18	12,672,843 (GRCm39)	missense	probably benign	0.05
R7150:Lama3	UTSW	18	12,601,346 (GRCm39)	missense	probably damaging	1.00
R7158:Lama3	UTSW	18	12,589,869 (GRCm39)	missense	probably benign	0.20
R7170:Lama3	UTSW	18	12,537,133 (GRCm39)	missense	probably benign	0.26
R7216:Lama3	UTSW	18	12,563,057 (GRCm39)	missense	probably damaging	1.00
R7223:Lama3	UTSW	18	12,715,665 (GRCm39)	missense	possibly damaging	0.53
R7243:Lama3	UTSW	18	12,552,902 (GRCm39)	missense	probably damaging	1.00
R7282:Lama3	UTSW	18	12,572,449 (GRCm39)	missense	probably damaging	0.99
R7337:Lama3	UTSW	18	12,640,097 (GRCm39)	splice site	probably null
R7442:Lama3	UTSW	18	12,605,238 (GRCm39)	critical splice acceptor site	probably null
R7487:Lama3	UTSW	18	12,552,294 (GRCm39)	missense	probably benign
R7604:Lama3	UTSW	18	12,633,550 (GRCm39)	missense	possibly damaging	0.93
R7609:Lama3	UTSW	18	12,664,891 (GRCm39)	critical splice acceptor site	probably null
R7650:Lama3	UTSW	18	12,670,895 (GRCm39)	missense	probably benign	0.01
R7894:Lama3	UTSW	18	12,595,864 (GRCm39)	missense	probably benign	0.07
R7975:Lama3	UTSW	18	12,670,796 (GRCm39)	missense	probably damaging	1.00
R8099:Lama3	UTSW	18	12,667,120 (GRCm39)	missense	probably damaging	0.97
R8168:Lama3	UTSW	18	12,639,999 (GRCm39)	missense	probably null
R8219:Lama3	UTSW	18	12,572,417 (GRCm39)	missense	probably benign	0.07
R8227:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8229:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8298:Lama3	UTSW	18	12,658,910 (GRCm39)	missense	probably benign	0.12
R8351:Lama3	UTSW	18	12,673,670 (GRCm39)	missense	probably damaging	1.00
R8364:Lama3	UTSW	18	12,661,404 (GRCm39)	missense	probably damaging	0.99
R8463:Lama3	UTSW	18	12,582,896 (GRCm39)	missense	probably damaging	0.96
R8515:Lama3	UTSW	18	12,544,688 (GRCm39)	missense	probably null	0.01
R8784:Lama3	UTSW	18	12,554,212 (GRCm39)	missense	probably benign
R8799:Lama3	UTSW	18	12,624,000 (GRCm39)	missense	probably damaging	0.96
R8874:Lama3	UTSW	18	12,582,643 (GRCm39)	critical splice donor site	probably null
R8938:Lama3	UTSW	18	12,689,762 (GRCm39)	missense	probably damaging	1.00
R8967:Lama3	UTSW	18	12,665,096 (GRCm39)	missense	possibly damaging	0.46
R9039:Lama3	UTSW	18	12,614,120 (GRCm39)	nonsense	probably null
R9126:Lama3	UTSW	18	12,583,527 (GRCm39)	missense	probably damaging	1.00
R9200:Lama3	UTSW	18	12,605,297 (GRCm39)	missense	probably benign	0.00
R9203:Lama3	UTSW	18	12,595,869 (GRCm39)	missense	probably benign	0.04
R9246:Lama3	UTSW	18	12,710,959 (GRCm39)	missense	probably damaging	0.99
R9284:Lama3	UTSW	18	12,583,541 (GRCm39)	nonsense	probably null
R9553:Lama3	UTSW	18	12,563,019 (GRCm39)	missense	probably damaging	1.00
R9716:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R9734:Lama3	UTSW	18	12,682,320 (GRCm39)	missense	possibly damaging	0.94
X0019:Lama3	UTSW	18	12,715,631 (GRCm39)	missense	possibly damaging	0.94
Z1177:Lama3	UTSW	18	12,562,936 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- AACCCTGACTTCTTCACAGTCAG -3'
(R):5'- CCTCTGAGGGCGTATGTTAC -3'

Sequencing Primer
(F):5'- CTGACTTCTTCACAGTCAGAATTGTG -3'
(R):5'- CTCTGAGGGCGTATGTTACAACAC -3'

Posted On

2015-03-18