Incidental Mutation 'R0543:Nkiras2'
ID271569
Institutional Source Beutler Lab
Gene Symbol Nkiras2
Ensembl Gene ENSMUSG00000017837
Gene NameNFKB inhibitor interacting Ras-like protein 2
SynonymsKBRAS2, 4930527H08Rik, 2410003M04Rik
MMRRC Submission 038735-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R0543 (G1)
Quality Score225
Status Validated
Chromosome11
Chromosomal Location100619244-100627607 bp(+) (GRCm38)
Type of Mutationunclassified
DNA Base Change (assembly) G to A at 100624192 bp
ZygosityHeterozygous
Amino Acid Change
Gene Model predicted gene model for transcript(s): [ENSMUST00000014339] [ENSMUST00000017981] [ENSMUST00000051947] [ENSMUST00000103119] [ENSMUST00000107376] [ENSMUST00000142993]
Predicted Effect probably benign
Transcript: ENSMUST00000014339
SMART Domains Protein: ENSMUSP00000014339
Gene: ENSMUSG00000014195

DomainStartEndE-ValueType
TPR 28 61 3.39e-7 SMART
TPR 62 95 1.11e-2 SMART
TPR 96 129 4.09e-1 SMART
Blast:TPR 142 175 4e-13 BLAST
Blast:TPR 176 209 1e-13 BLAST
TPR 210 243 2.29e-4 SMART
TPR 256 289 1.11e-2 SMART
TPR 294 327 2.87e-5 SMART
TPR 328 361 1.83e-3 SMART
DnaJ 380 443 2.93e-29 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000017981
SMART Domains Protein: ENSMUSP00000017981
Gene: ENSMUSG00000017837

DomainStartEndE-ValueType
Pfam:Arf 1 168 4.3e-9 PFAM
Pfam:Roc 6 124 2.2e-13 PFAM
Pfam:MMR_HSR1 6 165 3.1e-6 PFAM
Pfam:Ras 6 170 2.9e-27 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000051947
SMART Domains Protein: ENSMUSP00000059559
Gene: ENSMUSG00000017837

DomainStartEndE-ValueType
Pfam:Arf 1 168 5.6e-9 PFAM
Pfam:Miro 6 123 2.2e-21 PFAM
Pfam:Ras 6 170 4.5e-27 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000103119
SMART Domains Protein: ENSMUSP00000099408
Gene: ENSMUSG00000014198

DomainStartEndE-ValueType
low complexity region 2 24 N/A INTRINSIC
ZnF_U1 72 108 4.36e-2 SMART
ZnF_C2H2 77 99 1.51e0 SMART
low complexity region 125 141 N/A INTRINSIC
low complexity region 143 161 N/A INTRINSIC
low complexity region 181 200 N/A INTRINSIC
ZnF_U1 225 259 5.72e-4 SMART
ZnF_C2H2 228 252 7.11e0 SMART
ZnF_U1 294 328 7.44e-3 SMART
ZnF_C2H2 297 321 4.34e0 SMART
low complexity region 347 365 N/A INTRINSIC
low complexity region 382 405 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000107376
SMART Domains Protein: ENSMUSP00000102999
Gene: ENSMUSG00000017837

DomainStartEndE-ValueType
Pfam:Arf 1 168 5.6e-9 PFAM
Pfam:Miro 6 123 2.2e-21 PFAM
Pfam:Ras 6 170 4.5e-27 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000127547
Predicted Effect noncoding transcript
Transcript: ENSMUST00000132886
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137688
Predicted Effect probably benign
Transcript: ENSMUST00000142993
SMART Domains Protein: ENSMUSP00000114456
Gene: ENSMUSG00000017837

DomainStartEndE-ValueType
Pfam:Arf 1 151 1.3e-8 PFAM
Pfam:Miro 6 123 1.4e-21 PFAM
Pfam:MMR_HSR1 6 145 4.5e-6 PFAM
Pfam:Ras 6 153 2.9e-25 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000154972
Predicted Effect probably benign
Transcript: ENSMUST00000155152
SMART Domains Protein: ENSMUSP00000116793
Gene: ENSMUSG00000014195

DomainStartEndE-ValueType
TPR 19 52 3.39e-7 SMART
TPR 53 86 1.11e-2 SMART
Blast:TPR 95 128 1e-13 BLAST
Blast:TPR 129 162 3e-14 BLAST
TPR 163 196 2.29e-4 SMART
TPR 209 242 1.11e-2 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000155840
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.4%
  • 10x: 96.5%
  • 20x: 93.3%
Validation Efficiency 99% (69/70)
Allele List at MGI
Other mutations in this stock
Total: 70 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1500015O10Rik C A 1: 43,742,289 N110K possibly damaging Het
4930444G20Rik T C 10: 22,066,924 S386G possibly damaging Het
4932438A13Rik G A 3: 36,996,458 S2981N probably benign Het
Alox5 T A 6: 116,454,317 probably null Het
Apol9b A G 15: 77,735,640 N212S probably damaging Het
Ash1l T A 3: 89,063,778 probably null Het
Ccdc180 A T 4: 45,900,041 K200* probably null Het
Ccser2 A T 14: 36,940,192 M345K probably benign Het
Cdcp2 A T 4: 107,097,676 probably null Het
Clca3a1 T C 3: 144,748,394 probably benign Het
Cntn3 G A 6: 102,269,090 probably benign Het
Col28a1 T A 6: 8,075,326 probably benign Het
Dock2 A G 11: 34,294,325 F1035S probably damaging Het
Dsg1a A T 18: 20,340,863 S998C probably damaging Het
Enox1 T C 14: 77,506,959 probably benign Het
Fgfr3 A G 5: 33,729,710 M1V probably null Het
Fuca2 T A 10: 13,503,126 Y5N probably damaging Het
Git2 G T 5: 114,745,531 H42Q probably damaging Het
Gm7964 G A 7: 83,756,394 noncoding transcript Het
Hars2 G A 18: 36,789,424 E337K probably damaging Het
Hells A G 19: 38,967,750 R797G probably benign Het
Hnf1a G A 5: 114,950,744 S571L probably benign Het
Hoxa5 T C 6: 52,204,340 Y4C probably damaging Het
Inpp4a G A 1: 37,369,492 probably benign Het
Ints6 T C 14: 62,696,611 I816V probably damaging Het
Itpr1 T C 6: 108,515,748 probably benign Het
Kcnt2 C A 1: 140,609,614 P1037T probably damaging Het
Lyg2 T A 1: 37,911,107 M47L possibly damaging Het
Macf1 G T 4: 123,376,378 A4648D probably damaging Het
Mcf2l T C 8: 12,996,728 probably null Het
Mcm9 C T 10: 53,541,598 R3H probably damaging Het
Met T A 6: 17,491,970 Y244N probably damaging Het
Mettl14 A T 3: 123,374,762 C210S possibly damaging Het
Mrgpra4 T C 7: 47,981,310 Y181C probably benign Het
Mtch2 T C 2: 90,849,682 V86A possibly damaging Het
Mttp A T 3: 138,111,696 I446N possibly damaging Het
Muc4 T A 16: 32,756,746 S2207T unknown Het
Muc5b A G 7: 141,851,785 T944A unknown Het
Myo15 A T 11: 60,479,051 H879L probably benign Het
Nostrin T G 2: 69,189,131 *507E probably null Het
Nup205 T C 6: 35,198,969 V589A probably benign Het
Olfr1141 C A 2: 87,753,650 L114F probably damaging Het
Olfr1444 G T 19: 12,861,888 V38F probably benign Het
Olfr530 A G 7: 140,373,394 I72T probably benign Het
Oxct2b T A 4: 123,116,989 M234K possibly damaging Het
Park2 G A 17: 11,067,179 D20N probably damaging Het
Pcdha1 A T 18: 37,185,068 I945F probably damaging Het
Pdzd3 A C 9: 44,248,934 H324Q probably damaging Het
Pik3ca G A 3: 32,450,261 probably null Het
Pkhd1l1 A G 15: 44,523,491 probably null Het
Plscr1 A T 9: 92,258,046 probably null Het
Psd T C 19: 46,319,517 E684G possibly damaging Het
Rab11fip3 T C 17: 25,994,225 E870G probably damaging Het
Rpl22l1 C A 3: 28,807,274 Y103* probably null Het
Slc38a4 A T 15: 97,016,839 N44K possibly damaging Het
Slco6c1 T A 1: 97,127,898 I93F probably damaging Het
Ssfa2 T A 2: 79,644,506 S270T possibly damaging Het
Strip1 G A 3: 107,626,775 T181M possibly damaging Het
Stxbp5l G A 16: 37,208,096 A535V probably damaging Het
Tg A T 15: 66,729,597 Q152L probably benign Het
Thada T C 17: 84,423,163 T1036A probably damaging Het
Tnfrsf21 C T 17: 43,038,213 H239Y probably benign Het
Tns1 T A 1: 73,952,697 T941S probably benign Het
Tppp3 T C 8: 105,468,208 D97G probably benign Het
Trp53bp1 C A 2: 121,251,868 A317S probably null Het
Trpm7 T C 2: 126,848,529 I210V probably damaging Het
Ubr1 A G 2: 120,881,093 L1440P probably damaging Het
Utp18 A T 11: 93,875,835 Y317N probably damaging Het
Zdhhc5 T A 2: 84,692,480 probably benign Het
Zfp719 A G 7: 43,589,253 probably null Het
Other mutations in Nkiras2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00434:Nkiras2 APN 11 100624982 missense probably damaging 0.97
IGL03080:Nkiras2 APN 11 100624279 unclassified probably null
R0733:Nkiras2 UTSW 11 100624932 unclassified probably null
R1192:Nkiras2 UTSW 11 100625980 unclassified probably null
R1698:Nkiras2 UTSW 11 100625163 missense probably damaging 1.00
R5727:Nkiras2 UTSW 11 100625027 missense probably damaging 1.00
R5973:Nkiras2 UTSW 11 100626040 missense probably damaging 0.99
R6427:Nkiras2 UTSW 11 100625035 missense probably damaging 1.00
Predicted Primers
Posted On2015-03-23