Mutagenetix > Incidental Mutations

Incidental Mutation 'R3150:Vmn2r32'

271623

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r32

Ensembl Gene

ENSMUSG00000096743

Gene Name

vomeronasal 2, receptor 32

Synonyms

V2r5

MMRRC Submission

040602-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.144) question?

Stock #

R3150 (G1)

Quality Score

116

Status

Not validated

Chromosome

Chromosomal Location

7463015-7479973 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 7472555 bp

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 443 (Y443C)

Ref Sequence

ENSEMBL: ENSMUSP00000092462 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094866]

Predicted Effect

probably benign
Transcript: ENSMUST00000094866
AA Change: Y443C

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000092462
Gene: ENSMUSG00000096743
AA Change: Y443C

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	3.6e-33	PFAM
Pfam:NCD3G	512	565	8.1e-21	PFAM
Pfam:7tm_3	598	833	9.5e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9530053A07Rik	C	A	7: 28,154,195	T1528N	probably benign	Het
Akna	A	T	4: 63,395,353	S178T	possibly damaging	Het
BC067074	A	T	13: 113,351,760	Q105H	probably damaging	Het
Cabin1	A	G	10: 75,656,911	L1850P	probably damaging	Het
Ccdc178	G	T	18: 22,067,652	A416E	possibly damaging	Het
Ces1g	C	T	8: 93,325,816	V282I	probably benign	Het
Col4a3	T	G	1: 82,657,137		probably null	Het
Crat	C	T	2: 30,413,859		probably null	Het
Csf2ra	C	A	19: 61,227,320	A16S	possibly damaging	Het
Cyp4f18	T	C	8: 71,993,200	D317G	possibly damaging	Het
Ddb1	T	A	19: 10,612,982	M291K	probably benign	Het
Gfod2	C	T	8: 105,717,221	G230D	probably benign	Het
Git2	A	G	5: 114,730,349	S257P	probably damaging	Het
Gm5592	A	G	7: 41,288,380	E362G	probably benign	Het
Gpatch2l	A	G	12: 86,244,315	T91A	possibly damaging	Het
Hjurp	A	G	1: 88,266,561		probably benign	Het
Hnrnph1	T	A	11: 50,385,792	V439E	probably benign	Het
Itgad	C	A	7: 128,190,981	H651N	possibly damaging	Het
Map3k20	C	T	2: 72,371,992	T189M	probably damaging	Het
Mapk11	T	C	15: 89,145,450		probably null	Het
Mrc2	G	A	11: 105,348,431		probably null	Het
Nmral1	G	A	16: 4,716,469	T36I	probably damaging	Het
Olfr1231	C	T	2: 89,303,218	V125M	possibly damaging	Het
Olfr1475	A	G	19: 13,479,460	V246A	probably damaging	Het
Olfr860	A	G	9: 19,846,214	I135T	possibly damaging	Het
Padi6	A	G	4: 140,735,389	L307P	probably damaging	Het
Pkd1	G	T	17: 24,579,791	R2691L	probably benign	Het
Ppp2r2a	G	A	14: 67,023,765	R169W	probably damaging	Het
Prdm1	A	T	10: 44,458,492		probably null	Het
Robo1	C	T	16: 72,970,269	P443L	possibly damaging	Het
Rtn4	CGAGGAGGAGGAGGAGGA	CGAGGAGGAGGAGGA	11: 29,693,308		probably benign	Het
Shprh	A	G	10: 11,170,030	H865R	probably damaging	Het
Spats1	A	T	17: 45,464,554	S15T	probably damaging	Het
Srgap2	T	C	1: 131,292,589	T216A	probably benign	Het
Sry	ACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTG	ACTGCTGCTGCTGCTGCTGCTGCTGCTGCTG	Y: 2,662,944		probably benign	Het
Tie1	G	A	4: 118,475,825	A902V	probably damaging	Het
Usp22	T	C	11: 61,160,581	Q312R	probably damaging	Het
Vps13d	A	C	4: 145,086,790	D3274E	probably damaging	Het
Wdr62	A	T	7: 30,271,670	N167K	possibly damaging	Het
Xpo5	A	G	17: 46,242,247		probably null	Het
Zswim7	A	T	11: 62,273,785	I43N	possibly damaging	Het
Zswim9	T	C	7: 13,277,270	T51A	possibly damaging	Het

Other mutations in Vmn2r32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02140:Vmn2r32	APN	7	7476697	missense	probably damaging	0.97
IGL02333:Vmn2r32	APN	7	7464144	missense	probably damaging	1.00
IGL02406:Vmn2r32	APN	7	7476710	missense	probably benign
IGL02428:Vmn2r32	APN	7	7474284	missense	probably benign	0.02
IGL02484:Vmn2r32	APN	7	7464117	missense	probably damaging	1.00
IGL03277:Vmn2r32	APN	7	7474252	missense	probably benign	0.23
IGL03366:Vmn2r32	APN	7	7464030	missense	probably damaging	0.99
R1055:Vmn2r32	UTSW	7	7474327	nonsense	probably null
R1695:Vmn2r32	UTSW	7	7463992	missense	probably benign	0.01
R2172:Vmn2r32	UTSW	7	7474615	missense	probably damaging	0.99
R2262:Vmn2r32	UTSW	7	7474619	missense	probably benign
R4362:Vmn2r32	UTSW	7	7479858	nonsense	probably null
R4432:Vmn2r32	UTSW	7	7479919	missense	probably damaging	0.98
R4851:Vmn2r32	UTSW	7	7479954	missense	possibly damaging	0.59
R4949:Vmn2r32	UTSW	7	7464084	missense	probably benign	0.22
R5990:Vmn2r32	UTSW	7	7479810	missense	probably damaging	0.97
R6083:Vmn2r32	UTSW	7	7464210	missense	probably benign	0.15
R6084:Vmn2r32	UTSW	7	7464210	missense	probably benign	0.15
R6116:Vmn2r32	UTSW	7	7464093	missense	probably damaging	1.00
R6263:Vmn2r32	UTSW	7	7476692	missense	possibly damaging	0.90
R6889:Vmn2r32	UTSW	7	7472574	missense	possibly damaging	0.82
R7286:Vmn2r32	UTSW	7	7479808	missense	probably benign	0.21
R7390:Vmn2r32	UTSW	7	7479852	missense	probably benign	0.00
R7412:Vmn2r32	UTSW	7	7474213	missense	possibly damaging	0.53
R7508:Vmn2r32	UTSW	7	7467374	missense	possibly damaging	0.87
Z1177:Vmn2r32	UTSW	7	7474161	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- ACGTATAAGTGAGAGTGTCGAC -3'
(R):5'- AAAAGATGTGAGCCTCTCTCC -3'

Sequencing Primer
(F):5'- CCATGTGGTAAATATGGGCTGAAC -3'
(R):5'- ATGTGAGCCTCTCTCCGTGTATG -3'

Posted On

2015-03-25