Mutagenetix > Incidental Mutations

Incidental Mutation 'R3781:Zfp658'

272048

Institutional Source

Beutler Lab

Gene Symbol

Zfp658

Ensembl Gene

ENSMUSG00000056592

Gene Name

zinc finger protein 658

Synonyms

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3781 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

43562256-43575461 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 43573846 bp

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 515 (R515H)

Ref Sequence

ENSEMBL: ENSMUSP00000103606 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000005597] [ENSMUST00000107972]

Predicted Effect

probably benign
Transcript: ENSMUST00000005597
AA Change: R515H

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000005597
Gene: ENSMUSG00000056592
AA Change: R515H

Domain	Start	End	E-Value	Type
KRAB	23	83	3.7e-27	SMART
ZnF_C2H2	276	295	8.84e1	SMART
ZnF_C2H2	301	323	5.81e-2	SMART
ZnF_C2H2	328	350	1.5e-4	SMART
ZnF_C2H2	356	378	1.84e-4	SMART
ZnF_C2H2	384	406	3.16e-3	SMART
ZnF_C2H2	412	434	1.45e-2	SMART
ZnF_C2H2	440	462	4.87e-4	SMART
ZnF_C2H2	468	490	4.87e-4	SMART
ZnF_C2H2	496	518	6.88e-4	SMART
ZnF_C2H2	524	546	1.69e-3	SMART
ZnF_C2H2	552	574	9.73e-4	SMART
ZnF_C2H2	580	602	1.82e-3	SMART
ZnF_C2H2	608	630	3.16e-3	SMART
ZnF_C2H2	636	658	8.94e-3	SMART
ZnF_C2H2	664	686	3.74e-5	SMART
ZnF_C2H2	692	714	2.99e-4	SMART
ZnF_C2H2	720	742	5.21e-4	SMART
ZnF_C2H2	748	770	2.79e-4	SMART
ZnF_C2H2	776	798	1.58e-3	SMART
ZnF_C2H2	804	826	3.44e-4	SMART
ZnF_C2H2	832	854	5.59e-4	SMART
ZnF_C2H2	860	882	5.81e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107972
AA Change: R515H

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000103606
Gene: ENSMUSG00000056592
AA Change: R515H

Domain	Start	End	E-Value	Type
KRAB	23	83	3.7e-27	SMART
ZnF_C2H2	276	295	8.84e1	SMART
ZnF_C2H2	301	323	5.81e-2	SMART
ZnF_C2H2	328	350	1.5e-4	SMART
ZnF_C2H2	356	378	1.84e-4	SMART
ZnF_C2H2	384	406	3.16e-3	SMART
ZnF_C2H2	412	434	1.45e-2	SMART
ZnF_C2H2	440	462	4.87e-4	SMART
ZnF_C2H2	468	490	4.87e-4	SMART
ZnF_C2H2	496	518	6.88e-4	SMART
ZnF_C2H2	524	546	1.69e-3	SMART
ZnF_C2H2	552	574	9.73e-4	SMART
ZnF_C2H2	580	602	1.82e-3	SMART
ZnF_C2H2	608	630	3.16e-3	SMART
ZnF_C2H2	636	658	8.94e-3	SMART
ZnF_C2H2	664	686	3.74e-5	SMART
ZnF_C2H2	692	714	2.99e-4	SMART
ZnF_C2H2	720	742	5.21e-4	SMART
ZnF_C2H2	748	770	2.79e-4	SMART
ZnF_C2H2	776	798	1.58e-3	SMART
ZnF_C2H2	804	826	3.44e-4	SMART
ZnF_C2H2	832	854	5.59e-4	SMART
ZnF_C2H2	860	882	5.81e-2	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 23 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Bap1	T	C	14: 31,257,618	I526T	possibly damaging	Het
Calcr	T	C	6: 3,700,193	T263A	possibly damaging	Het
Cdh7	T	A	1: 110,049,004	V133E	probably benign	Het
Ddx5	T	C	11: 106,784,520	I330V	probably benign	Het
Dysf	G	A	6: 84,186,509		probably null	Het
Gnl2	T	C	4: 125,037,606	V110A	probably damaging	Het
H2-Q10	A	G	17: 35,471,018	Y179C	possibly damaging	Het
Hlx	G	T	1: 184,731,987	A52D	probably damaging	Het
Map3k20	A	G	2: 72,402,355		probably benign	Het
Mcm7	G	A	5: 138,164,736	R385W	probably damaging	Het
Mgat4c	A	G	10: 102,388,921	E332G	probably benign	Het
Nudt3	A	G	17: 27,580,808	S134P	possibly damaging	Het
Olfr1186	A	T	2: 88,526,365	T261S	probably benign	Het
Olfr1212	G	T	2: 88,958,747	E94*	probably null	Het
Olfr385	C	A	11: 73,589,013	G242C	probably damaging	Het
Olfr385	A	T	11: 73,589,368	Y123*	probably null	Het
Olfr573-ps1	T	C	7: 102,942,071	I169V	probably benign	Het
Pcnx	A	G	12: 81,996,118	T2325A	probably benign	Het
Plekha6	A	G	1: 133,294,655	E993G	probably damaging	Het
Psmd4	T	A	3: 95,036,728	Y15F	probably benign	Het
Rad23b	C	T	4: 55,382,586	T263M	probably damaging	Het
Stpg3	T	A	2: 25,213,863	M154L	probably benign	Het
Vmn1r45	C	A	6: 89,933,817	R57L	probably benign	Het

Other mutations in Zfp658

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Zfp658	APN	7	43567356	missense	probably benign	0.03
IGL00475:Zfp658	APN	7	43574076	missense	possibly damaging	0.68
IGL01972:Zfp658	APN	7	43572710	nonsense	probably null
IGL03223:Zfp658	APN	7	43567311	missense	possibly damaging	0.85
R0135:Zfp658	UTSW	7	43573595	nonsense	probably null
R1863:Zfp658	UTSW	7	43573899	missense	possibly damaging	0.78
R1962:Zfp658	UTSW	7	43573821	missense	possibly damaging	0.93
R2698:Zfp658	UTSW	7	43573545	missense	possibly damaging	0.53
R4791:Zfp658	UTSW	7	43574466	missense	possibly damaging	0.93
R5392:Zfp658	UTSW	7	43572931	missense	probably benign	0.07
R6092:Zfp658	UTSW	7	43574527	missense	possibly damaging	0.73
R6594:Zfp658	UTSW	7	43567277	missense	possibly damaging	0.86
R7003:Zfp658	UTSW	7	43574748	missense	possibly damaging	0.85
R7008:Zfp658	UTSW	7	43573912	missense	possibly damaging	0.95
R7077:Zfp658	UTSW	7	43573989	missense	probably benign	0.32
R7689:Zfp658	UTSW	7	43574678	missense	probably benign	0.00
R7793:Zfp658	UTSW	7	43574684	missense	possibly damaging	0.74
R7939:Zfp658	UTSW	7	43574877	missense	possibly damaging	0.73
Z1176:Zfp658	UTSW	7	43573217	missense	possibly damaging	0.72

Predicted Primers

PCR Primer
(F):5'- AGCCTCACCGTTCACCAG -3'
(R):5'- GACCGTCAGCTGGGATGTG -3'

Sequencing Primer
(F):5'- CGTTCACCAGAAGATCCATATCAG -3'
(R):5'- TCAGCTGTGACCTCTGAAAG -3'

Posted On

2015-03-25