Mutagenetix > Incidental Mutation

Incidental Mutation 'R3787:Or8g32'

272320

Institutional Source

Beutler Lab

Gene Symbol

Or8g32

Ensembl Gene

ENSMUSG00000094269

Gene Name

olfactory receptor family 8 subfamily G member 32

Synonyms

GA_x6K02T2PVTD-33090395-33091330, MOR171-33P, MOR171-49, Olfr951

MMRRC Submission

040754-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R3787 (G1)

Quality Score

153

Status

Not validated

Chromosome

Chromosomal Location

39305089-39306033 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 39305678 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 197 (V197A)

Ref Sequence

ENSEMBL: ENSMUSP00000077615 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078531] [ENSMUST00000216107]

AlphaFold

Q9EQ94

Predicted Effect

probably benign
Transcript: ENSMUST00000078531
AA Change: V197A

PolyPhen 2 Score 0.066 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000077615
Gene: ENSMUSG00000094269
AA Change: V197A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	311	2.5e-52	PFAM
Pfam:7tm_1	44	293	5e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216107
AA Change: V194A

PolyPhen 2 Score 0.031 (Sensitivity: 0.95; Specificity: 0.82)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110082I17Rik	G	T	5: 139,441,197 (GRCm39)	P35Q	probably damaging	Het
Aoc1	T	C	6: 48,882,589 (GRCm39)	L177P	probably damaging	Het
Aprt	T	C	8: 123,302,268 (GRCm39)	D65G	probably benign	Het
Auh	C	A	13: 53,083,493 (GRCm39)	R62L	possibly damaging	Het
Bmp4	C	T	14: 46,623,171 (GRCm39)		probably null	Het
Bptf	C	A	11: 106,964,653 (GRCm39)	D1514Y	probably damaging	Het
Carmil3	T	C	14: 55,734,433 (GRCm39)	F418S	probably damaging	Het
Ccdc112	C	T	18: 46,432,365 (GRCm39)	R72H	probably benign	Het
Ccdc138	T	C	10: 58,374,092 (GRCm39)	Y371H	probably damaging	Het
Chsy3	T	C	18: 59,542,070 (GRCm39)	Y403H	probably damaging	Het
Cul4a	T	C	8: 13,183,668 (GRCm39)	V352A	probably damaging	Het
Dennd3	T	C	15: 73,419,426 (GRCm39)	V739A	possibly damaging	Het
Dmxl1	G	A	18: 49,998,189 (GRCm39)	S763N	probably damaging	Het
Dmxl2	T	C	9: 54,277,162 (GRCm39)	D2893G	probably damaging	Het
Dnah8	A	G	17: 30,974,015 (GRCm39)	D2800G	probably damaging	Het
Dnaja2	C	T	8: 86,267,015 (GRCm39)	G281R	probably damaging	Het
Exo1	A	G	1: 175,727,035 (GRCm39)	T449A	probably benign	Het
Fancd2	T	G	6: 113,542,165 (GRCm39)	S770A	probably damaging	Het
Fmo1	A	T	1: 162,657,583 (GRCm39)	S519R	possibly damaging	Het
Fryl	T	C	5: 73,258,819 (GRCm39)	Y655C	probably benign	Het
Gpt2	G	T	8: 86,252,202 (GRCm39)	V506L	probably benign	Het
Heatr1	G	T	13: 12,449,341 (GRCm39)	L1946F	probably damaging	Het
Inpp5k	T	C	11: 75,538,512 (GRCm39)	L461P	probably damaging	Het
Magi2	C	T	5: 20,670,907 (GRCm39)	T580M	probably damaging	Het
Mcm9	A	G	10: 53,492,076 (GRCm39)	V415A	possibly damaging	Het
Mki67	A	T	7: 135,302,012 (GRCm39)	N1007K	possibly damaging	Het
Mpped1	A	T	15: 83,680,784 (GRCm39)		probably benign	Het
Mtpap	T	C	18: 4,380,670 (GRCm39)	V116A	probably damaging	Het
Myo15a	A	T	11: 60,368,398 (GRCm39)	Y386F	probably damaging	Het
Neurod1	T	A	2: 79,284,939 (GRCm39)	N148I	probably damaging	Het
Nfs1	A	G	2: 155,970,503 (GRCm39)	I270T	possibly damaging	Het
Nr1i3	A	G	1: 171,041,994 (GRCm39)	D26G	probably damaging	Het
Nsa2	G	T	13: 97,272,042 (GRCm39)	Q60K	possibly damaging	Het
Or13a28	G	A	7: 140,217,748 (GRCm39)	V45I	probably benign	Het
Pde4dip	G	T	3: 97,622,868 (GRCm39)	P1447Q	possibly damaging	Het
Plxna2	A	G	1: 194,326,242 (GRCm39)	T59A	probably benign	Het
Pmepa1	G	A	2: 173,069,926 (GRCm39)	R210W	probably damaging	Het
Ppp1r12c	G	A	7: 4,489,583 (GRCm39)	A193V	probably damaging	Het
Pramel48	T	A	5: 95,630,756 (GRCm39)	L211Q	probably damaging	Het
Prdm15	G	T	16: 97,598,945 (GRCm39)	H904Q	probably benign	Het
Rala	T	A	13: 18,057,031 (GRCm39)	E185V	probably benign	Het
Reep1	T	A	6: 71,772,199 (GRCm39)	D162E	probably damaging	Het
Rev3l	T	A	10: 39,722,206 (GRCm39)	L2528Q	probably damaging	Het
Rfc1	A	T	5: 65,453,357 (GRCm39)	S264T	probably benign	Het
Sall4	A	G	2: 168,598,043 (GRCm39)	S266P	probably damaging	Het
Sipa1l2	C	T	8: 126,149,944 (GRCm39)	A1602T	probably benign	Het
Sipa1l2	C	A	8: 126,177,122 (GRCm39)	C1164F	possibly damaging	Het
Slc4a1ap	T	A	5: 31,685,483 (GRCm39)	L254I	possibly damaging	Het
Slc5a3	A	G	16: 91,874,816 (GRCm39)	N291S	possibly damaging	Het
Stab2	T	A	10: 86,805,141 (GRCm39)	D279V	possibly damaging	Het
Synrg	T	C	11: 83,892,746 (GRCm39)	F613S	probably damaging	Het
Tekt1	A	G	11: 72,235,720 (GRCm39)	I376T	probably damaging	Het
Thbs4	T	C	13: 92,909,672 (GRCm39)	N375S	probably benign	Het
Tro	G	A	X: 149,438,048 (GRCm39)	T203I	possibly damaging	Het
Txnl4b	C	T	8: 110,299,409 (GRCm39)	A123V	probably damaging	Het
Vmn1r63	T	A	7: 5,805,751 (GRCm39)	M294L	probably benign	Het
Vmn2r58	C	T	7: 41,513,498 (GRCm39)	D382N	probably benign	Het
Wap	G	A	11: 6,588,550 (GRCm39)	Q25*	probably null	Het

Other mutations in Or8g32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01527:Or8g32	APN	9	39,305,114 (GRCm39)	missense	probably benign	0.01
IGL01650:Or8g32	APN	9	39,305,252 (GRCm39)	missense	probably damaging	0.99
IGL02134:Or8g32	APN	9	39,305,830 (GRCm39)	missense	probably damaging	0.99
IGL03113:Or8g32	APN	9	39,305,981 (GRCm39)	missense	probably damaging	1.00
R0127:Or8g32	UTSW	9	39,305,238 (GRCm39)	missense	probably benign	0.16
R1730:Or8g32	UTSW	9	39,305,518 (GRCm39)	missense	probably benign	0.01
R1783:Or8g32	UTSW	9	39,305,518 (GRCm39)	missense	probably benign	0.01
R1924:Or8g32	UTSW	9	39,305,163 (GRCm39)	missense	possibly damaging	0.93
R3785:Or8g32	UTSW	9	39,305,678 (GRCm39)	missense	probably benign	0.07
R4607:Or8g32	UTSW	9	39,306,031 (GRCm39)	makesense	probably null
R4803:Or8g32	UTSW	9	39,305,932 (GRCm39)	missense	probably benign	0.26
R5314:Or8g32	UTSW	9	39,305,785 (GRCm39)	missense	probably damaging	1.00
R5338:Or8g32	UTSW	9	39,305,371 (GRCm39)	missense	probably damaging	1.00
R5360:Or8g32	UTSW	9	39,305,698 (GRCm39)	missense	probably benign	0.00
R5468:Or8g32	UTSW	9	39,305,257 (GRCm39)	missense	probably benign	0.33
R6590:Or8g32	UTSW	9	39,305,845 (GRCm39)	missense	probably benign	0.00
R6690:Or8g32	UTSW	9	39,305,845 (GRCm39)	missense	probably benign	0.00
R6925:Or8g32	UTSW	9	39,305,157 (GRCm39)	missense	probably benign	0.01
R6925:Or8g32	UTSW	9	39,305,156 (GRCm39)	missense	probably benign	0.32
R6982:Or8g32	UTSW	9	39,305,618 (GRCm39)	missense	probably damaging	1.00
R7662:Or8g32	UTSW	9	39,305,389 (GRCm39)	missense	probably benign	0.01
R8074:Or8g32	UTSW	9	39,305,242 (GRCm39)	missense	probably damaging	1.00
R8389:Or8g32	UTSW	9	39,305,912 (GRCm39)	missense	probably damaging	1.00
R9444:Or8g32	UTSW	9	39,305,365 (GRCm39)	missense	probably benign	0.19
R9642:Or8g32	UTSW	9	39,305,857 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCCATGTCCTCTCAGATTTAC -3'
(R):5'- CCATTGAACTCACTTGTGATGGC -3'

Sequencing Primer
(F):5'- CAGATTTACTGTTCTCTGATTTCAGG -3'
(R):5'- CTCACTTGTGATGGCTGTAAGTACAC -3'

Posted On

2015-03-25